• 한국연초학회지
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• 제19권1호
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• pp.33-39
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• 1997

Recently the method of basal compound fertilizer (N : P : K = 18.2 : 9.8 : 35.0) Placement has been changed from banding to broadcasting in tobacco cultivation. The effects of the compound fertilizer placement, level of fertilizer and additional urea application on the agronomic characteristics, two chemical compositions and physical Properties of burley tobacco(Nicotiana tabacum L. cv. KB 108) were investigated at Chonju Experiment Station, Korea Ginseng and Tobacco Research Institute in 1995-1996. Six treatments consisted of (1) band (method of fertilizer Placement) + In kg/10a(compound fertilizer) + 0(additional urea applied), (2) broadcast+140+0, (3) broadcast+140+25, (4) band+180+0, (5) broadcast+180+0, and (6) broadcast +180+25. The additional urea was applied at hilling. No significant differences were detected between banding and broadcasting method of compound fertilizer Placement in field 9rowth, wield, organoleptic qualify(price Per kilogram), chemical composition and Physical properties of cured leaf, The increased compound fertilizer by 30%(40k/10a) or the additional urea application by 25kg per 10a produced slightly higher yield than the recommended amount of basal compound fertilizer without additional urea application did. It also increased the total alkaloid content of cured leaf. It is recommended that no more basal fertilizer above the recommended amount and no additional urea application are needed in burley tobacco fertilization, even though the method of basal fertilizer placement being chanced from banding to broadcasting. Key words : Nicotiana tabacum, fertilizer placement, additional urea.

• 농업과학연구
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• 제42권4호
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• pp.423-429
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• 2015

This study was conducted to evaluate the effects of reduced nitrogen (N) and potassium (K) fertigation as additional fertilizer on tomato yield and nutrient contents in excessively nutrients-accumulated soil. Shoot and root dry weights (DW), dry matter rate for shoot, root and fruit and number of fruit in both AF50 and AF100 (50 and 100% levels of additional fertilizer) treatments were increased in comparison with those in AF0 (0% level of additional fertilizer) treatment. In case of nutrient uptake by tomato, nitrogen, phosphorous (P) and potassium contents in all tomato parts (leaf, stem, root and fruit) in AF50 and AF100 treatment were lower than those in AF0 treatment. On the contrary, soluble sugar and starch contents in all tomato parts in AF50 and AF100 were higher than those in AF0 treatment. There were differences between AF0 and AF50 or AF100 in tomato growth, yield, nutrient level and contents of soluble sugar and starch. In contrast, the level and initiation point of fertigation did not significantly affect the parameters. Based on our results, the application of properly reduced level of additional fertilizer is possible to maintain the productivity of tomato and alleviate the nutrient accumulation in plastic film house soils.

• 한국토양비료학회지
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• 제48권1호
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• pp.30-35
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• 2015

This study was conducted to investigate investigated the effect of nitrogen fertilizer on nitrate concentration in soil solution and to determine the relationship between yield and nitrate concentration in soil solution for cucumber cultivation under plastic film house. Nitrogen as urea was applied at rates of 0, 120, 240, 360, and $480kg\;N\;ha^{-1}$ as an additional fertilizer by trickle irrigation during cucumber cultivation. Monitoring of nitrate concentration in soil solution was investigated using porous cups at 25 cm depth under soil surface. Nitrate concentration in soil solution increased with increasing the rate of additional nitrogen. Correlation coefficient between EC value and nitrate concentration was positive in soil and soil solution (p<0.05). An additional nitrogen of about $300kg\;ha^{-1}$ was shown the highest yield of cucumber, and improved yield by 5% compared to N recommendation of $240kg\;N\;ha^{-1}$. The highest yield was determined at nitrate concentration of $82mg\;L^{-1}$ in soil solution by regression equation ($Y=74.2+0.73X+0.000504X^2$, $R^2=0.629^*$). These results means indicate that nitrate concentration in soil solution would be useful method to rapid determination for additional nitrogen during cucumber cultivation under plastic film house.

• 한국토양비료학회지
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• 제49권6호
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• pp.699-704
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• 2016

An estimation of optimal requirement of additional N by cropping pattern and growth stage is very important for greenhouse watermelon. The objectives of this study were to estimate an amount of optimal additional N based on growth, N uptake and yield of watermelon. In order to achieve these goals, we performed the study at farmer's greenhouse with a fertigation system and watermelon was cultivated three times (spring, summer and autumn) in 2015. The levels of additional N were set up with x0.5, x0.75, x1.0 and x1.5 of the $NO_3$-N-based soil-testing N supply for watermelon cultivation. The trends of growth and N uptake of watermelon markedly differed from cropping pattern; spring (sigmoid), summer and autumn (linear). The yield of watermelon was the highest at summer season and followed by autumn and spring. Also, the x1.5N showed a significantly higher yield compared to other N treatments. On the basis of growth, N uptake and yield of watermelon, we estimated an optimal level of additional N by cropping pattern and growth stage as follows; 1) spring (transplanting ~ 6 WAT : 6 ~ 14 WAT : 14 ~ harvest = 5 : 90 : 5%), summer (transplanting ~ 4 WAT : 4 ~ 8 WAT : 8 ~ harvest = 25 : 50 : 25%) and autumn (transplanting ~ 4 WAT : 4 ~ harvesting : 50 : 50%). In conclusion, nutrient management, especially N, based on cropping pattern and growth stage was effective for favorable growth and yield of watermelon.

• 한국자원식물학회지
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• 제20권2호
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• pp.129-132
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• 2007

삼백초 1회 수확 후 질소 추비량에 따른 생육 및 수량을 검토하여 재배법 확립의 기초자료를 제공하고자, 무시용 등 4처리를 두어 2002년부터 3년간 시험한 결과, 초장, 엽의 크기, 경태, 절수, 상품엽수, 분얼수 등 경엽과 근경의 생육은 1차 수확 후 질소 추비량이 많을수록 길거나, 굵거나, 많은 경향으로 양호하였다. 경엽수량은 질소 추비량이 많을수록 증가되어 무시용 158kg/10a 대비 3, 6, 9kg/10a 사용 시 각각 20%, 42%, 60% 증수되었고, 성분함량 중 rutin은 질소 추비 사용량이 많을수록 증가되는 경향이었으며, quercitrin은 질소 추비 3kg/10a 시용에서 다소 높았다.

• 한국토양비료학회지
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• 제44권5호
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• pp.752-761
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• 2011

Recently, upland crops cultivation instead of paddy crops are more popular and highlighted by increase of social demand in agricultural land use. Especially, wheat cultivation for replacing of import food grain are more interested by government, and it is urgently needed that possibility of wheat cultivation is evaluated in the reclaimed tidal land. Crop cultivation is closely related with soil salinity and cultivation method in the reclaimed tidal land. In order to evaluate possibility of wheat cultivation, effect of different application level of compost and nitrogen additional fertilizer, also soil salinity on the grain yield and yield components of three wheat cultivars was studied at the newly reclaimed Saemangeum and Hwanong tidal lands in Korea. $270-300kg\;10a^{-1}$ of grain yield were obtained at the experimental site in the Saemangeum reclaimed tidal land where soil salinity was less than $4dS\;m^{-1}$ during growing periods from December, 2009 to June, 2010. However, almost no grain yield was obtained at the experimental site in the Hwaong reclaimed tidal land, where soil salinity was more than average $8dS\;m^{-1}$ ranged from 2.0 to $25.9dS\;m^{-1}$ during growing period and then salt demage was severe. Yield was significantly different among application level of compost and nitrogen additional fertilizer in the newly reclaimed Saemangeum tidal land. However, it is considered that three cultivars such as Chopum, Chogyung and Geumgang, have similar sensibility to soil salinity and fertilizer level, because there is statistically no difference among ciltivars in Hwaong and Saemangeum, and also among cultivars in the different levels of compost and fertilizer. Finally, it is concluded that wheat can be possibly produced by reasonable fertilizer application in the Saemangeum reclaimed tidal land, but wheat cultivation is impossible because of high soil salinity in the Hwaong reclaimed tidal land.

• 한국약용작물학회지
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• 제27권1호
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• pp.1-8
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• 2019

Background: This study was conducted to investigate the effects of fertilization with the application of mixed organic matter after cultivating green manure crops on the growth and saponin content of Codonopsis lanceolata Trautv. Methods and Results: Five type of cultivation of green manure crops were done: hairy vetch as a single seedling crop (HV), hairy vetch and rye as a mixture of seedling (HV/R), additional fertilization with mixed organic matter after cultivation of a seedling mixture (HV/R/MO), chemical fertilizer (CF), and a non-treated control (NT). The total nitrogen content was the highest in the HV/R/MO treatment, followed by the HV, CF, HV/R and NT treatments, respectively. Nitrogen fixation was higher in the single seedling treatment with hairy vetch (HV) than in the treatment with a mixture of hairy vetch and rye seedling (HV/R). Moreover, the growth of C. lanceolata was greatly increased by the application of additional fertilizer after treatment with the mixture of hairy vetch and rye seedlings. The HV/R/MO treatment produced the highest total lancemaside content among the treatments, followed by HV, HV/R, CF, and NT, respectively. Conclusions: It was verified that C. lanceolata experienced superior growth with the application of mixed organic matter as additional fertilizer after the cultivation of green manure crops, and the content of its major saponins, lancemasides were also increased by this treatment.

• 한국잠사곤충학회지
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• 제35권1호
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• pp.17-20
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• 1993

강원도 지방에서의 철늦은 뽕밭 덧거름이 뽕잎 수량과 봄철 가지끝마름에 미치는 영향을 구명하기 위하여 관행시비시기인 6월 20일을 대조로 8월 10일과 30일에 덧거름을 주고 수량과 가지끝마름비율을 조사한 결과 다음과 같았다. 1. 뽕잎수량은 8월 10일 시비구는 가장 높아 관행대비 10~30%, 잎뽕수확구에서는 10~17%의 증수를 보였으나, 통계적인 유의차는 인정되지 않았으며, 8월 30일구에서는 관행구와 큰 차를 보이지 않았다. 2. 봄철 가지끝마름비율을 비교하면, 관행구가 가장 높아 중간벌채구에서는 13.1%를 보인 반면 다른 구에서는 이보다 낮은 7.7~12.8%를, 잎뽕 수확구에서는 18.3%를 보인 반면 다른 구에서는 이보다 적은 11.0~13.3%를 보여서 덧거름에 의한 가지끝마름비율의 증가는 인정되지 않았다.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1717-1722
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• 2016

A novel phytase of Acidobacteria was identified from a soil metagenome, cloned, overexpressed, and purified. It has low sequence similarity (<44%) to all the known phytases. At the optimum pH (2.5), the phytase shows an activity level of 1,792 μmol/min/mg at physiological temperature (37℃) and could retain 92% residual activity after 30 min, indicating the phytase is acidophilic and acidostable. However the phytase shows poor stability at high temperatures. To improve its thermal resistance, the enzyme was redesigned using Disulfide by Design 2.0, introducing four additional disulfide bridges. The half-life time of the engineered phytase at 60℃ and 80℃, respectively, is 3.0× and 2.8× longer than the wild-type, and its activity and acidostability are not significantly affected.

• 생물환경조절학회지
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• 제29권2호
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• pp.181-188
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• 2020

본 연구는 국내 특산식물이며 관상소재로 개발이 가능한 산꼬리풀의 효과적인 육묘조건을 구명하기 위해 수행되었다. 육묘는 토양 종류와 비료 농도, 차광정도, 추비 농도, 종자의 광원 전처리 및 채종 시기별로 각 요인을 달리하여 수행하였다. 연구 결과, 산꼬리풀은 원예상토에서 생육이 우수하였으며, 혼용토에서는 비료 첨가 시 생육이 억제되었다. 차광은 55% 차광조건에서 무차광에 비해 전체적인 생육이 향상되었다. 고농도(1000mg·L^-1)의 Hyponex는 육묘 시 생육을 촉진하였으며, 파종 전 종자에 대한 적색광 조사는 추후 초장과 생체중 향상에 효과적이었다. 채종 시기는 2018년도 종자가 2017년 종자보다 생육이 우수하였다.