• 한국자원식물학회지
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• 제36권3호
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• pp.225-236
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• 2023

Stomatal traits such as stomata density (SD), aperture length (APL) and width (APW), guard cell length (GCL) and width (GCW), and distance between stomata (DIS) were investigated to identify correlation with agronomic traits for 35 Korean wheat cultivars. Flag leaf width (FLW) of Korean wheat cultivars was the widest in Ol-mil, and the narrowest in Keumkang. SD tended to be higher on the adaxial side than on the abaxial side in Korean wheat cultivars. SD of adaxial and abaxial sides was classified into a cultivar with a significantly different or not. In APL, 18 wheat cultivars showed significant differences according to leaf side, and APL of adaxial was longer than APL of abaxial in 13 wheat cultivars. In APW, 15 wheat cultivars showed a significant difference, and APW of abaxial was wider than APW of adaxial among them. In GCL, 14 wheat cultivars showed a significant difference, and the GCL of abaxial was longer than the GCL of adaxial in 10 wheat cultivars. In GCW, 10 wheat cultivars showed a significant difference, GCW of adaxial was wider than GCW of abaxial and in 6 wheat cultivars. FLW of adaxial and abaxial showed a negative correlation with GCL and a positive correlation with grain number per panicle. FLW of only abaxial showed a positive correlation with DIS. The SD of the adaxial showed a negative correlation with GCL, while the SD of the abaxial showed a negative correlation with APL. APL of both sides of the leaf showed a positive correlation with GCL, and APW of only abaxial showed a negative correlation with GLC. DIS of adaxial showed a negative correlation with tiller number (TN), while DIS of abaxial showed a positive correlation with GNP.

• Applied Microscopy
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• 제43권2호
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• pp.47-53
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• 2013

The trichomes on leaves of three species of Mentha such as M. spicata, M. suaveolens, and M. piperita var were examined by scanning electron microscopy. Simple non-glandular trichomes and peltate glandular trichomes were distinctively occurred on these leaves. In M. spicata, short and sharp non-glandular trichomes were dominantly appeared on adaxial surface, whereas capitate glandular trichomes were commonly localized on abaxial surface. In M. suaveolens, non-glandular trichomes were identified with simple unbranched and branched. Unbranched trichomes which were sharp and pointed in shape occurred on adaxial surface, however, branched and v-shaped trichomes appeared abundantly on abaxial surface. Peltate trichomes consisted of a large eight-celled head. Small capitates trichomes consisted of a cylindrical head with an one-celled uniseriate stalk. In M. piperita var, single non-glandular trichomes and peltate trichomes were present on adaxial surface. Small capitate trichomes consisted of a globose unicellular head with a two- or three-celled uniseriate stalk. Peltate trichomes were distinctly present on abaxial surface, whereas they were not observed on adaxial surface. The trichomes were less dense in this species. Peltate trichomes consisted of a large eight-celled head, with an enlarged secretory cavity, attached to an one-celled short stalk.

• 한국작물학회지
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• 제29권4호
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• pp.307-314
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• 1984

소맥 품종들에 대한 기공수의 품종간 차이, 엽각부위의 기공분석 및 기공수와 출수기, 간장및 타 광합성 관련형질들과의 관계를 구명코져 조광외 100품종을 공시 조사하였던 바 그 결과는 다음과 같다. 1. 지엽표면의 기공수는 최저 51(/$ extrm{mm}^2$), 최고90, 이면의 최저는 35, 최고는 65개로서 표면은 이면비해 1.5배가 많았으며, 가장 빈도가 높은 것은 기공수가 표면은 61~75, 이면은 41~50개인 품종들이었다. 2. 기공의 크기는 표면은 38.5~46.6$\mu$, 이면은39.4$\mu$48.0$\mu$으로 이면의 기공이 다소컸으며 기공수와 기공의 크기 간에는 표면 -0.63＊＊, 이면 -0.48＊의 상관이 인정되었다. 3. 상위엽의 기공수는 하위엽보다 많고 각 분얼 동신엽의 기공수는 지엽은 고위분얼이 그 하위엽에서는 저위분얼에서 발생한 엽에서 많아지는 경향이었다. 4. 동일엽신내에서는 표면은 선단부나 중앙부가 많고 기부가 적은 반면 이면은 반대의 경향을 나타내었다. 5. 지엽 및 제2엽의 엽초에도 기공수는 상당히 존재하여 제3엽이나 제4엽이면의 기공수보다도 많았다. 6. 지엽표면의 기공수가 많은 품종은 타엽신이나 엽초에도 많았다. 7. 지엽표면의 기공수와 간장, 출수기와는 각각 0.53＊, 0.51＊의 상관이 있었고 엽면적당 건물중(SLW)과는 -0.48＊, 엽록소함량 0.53＊, 엽면적 -0.59＊＊, 기공개도와는 -0.53＊ 의 상관이 인정되었으며, 엽맥수와는 상관이 인정되지 않았다. 따라서 기공수가 많은 품종은 간장이 크고, 출수기는 늦어지는 경향이며, 엽유의 두께나 엽면적은 작고 엽록소함량은 높아지는 경향이었다.

• 한국작물학회지
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• 제26권1호
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• pp.45-50
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• 1981

Diurnal changes in leaf stomatal resistances were measured on leaf positions and both surfaces to investigate the stomatal response to irradiance in wheat, var. Chokwang and barley, var. Dongbori 1. Stomatal frequency and size were also determined to explain the control mechanism of gas exchanges in two species. The leaf diffusive resistances of two species decreased, as the sun rose, to minimum at 10 to 11 o'clock a.m. and increased gradually in the afternoon, even faster at sunset. As the adaxial irradiance increased, stomatal resistances decreased sensitively in the range of 30uEm$^{-2}$ㆍsec$^{-1}$ to 150uEm$^{-2}$ㆍsec$^{-1}$ quantum flux density. The stomatal opening of the abaxial surface began at lower irradiance and was completed earlier than the adaxial surface. The adaxial irradiances decreased in order of leaf position, flag, the 2nd, the 3rd leaf, and the stomatal resistances increased in the same order. Even under the same irradiance, the stomatal resistance of lower leaves were higher than those of upper leaves. The stomatal frequencies of lower leaves were less, but the stomatal size was greater than those of upper leaves. Consequently, the relative leaf area occupied by stomatal pores were constant among leaf positions in two species.

• 원예과학기술지
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• 제31권5호
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• pp.590-597
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• 2013

장미 꽃의 향기정도에 따른 주요성분과 향기발산 위치를 구명하기 위하여 본 연구를 수행하였다. 향기정도는 약한 품종('필립'과 '비너스베리'), 중간 선발계통('GR07-135') 및 강한 품종('허니블루') 3수준으로 구분되었다. 품종과 선발계통 별 주요 향기성분은 '필립'은 3,5-dimethoxytoluene(DMT)와 benzene,1,3,5-trimethoxy 성분이었으며, '비너스베리'는 megastigma-4,6(Z),8(E)-triene 성분이었고, 'GR07-135'는 DMT, benzene,1-ethenyl-4-methoxy 및 phenylethylalcohol 성분이었으며, '허니블루'는 germacrene-D와 DMT 성분이었다. 향축면 표피세포는 원추형의 유두상 돌기 모양이 있었고 배축면 표피세포는 편평한 모양이었다. 3품종과 1선발계통 모두 두꺼운 세포벽이 있었고, '허니블루'를 제외한 2품종과 1선발계통은 왁스로 덮인 큐티클층이 있었다. 향축면 표피세포에 '필립'은 전분립이, '비너스베리'는 오스미움 소체가, 'GR07-135'은 전분립, 색소체 및 액포가, '허니블루'는 색소체낭, 색소체 및 액포가 있었다. 결과적으로 방향성 장미 꽃 향기의 주요성분은 품종에 따라 달랐으며, 생성과 발산은 유두돌기가 있는 향축면 표피 세포내의 색소체와 액포가 관련되어 있었다.

• 한국자원식물학회지
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• 제29권4호
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• pp.376-384
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• 2016

쉬땅나무족 15분류군의 화판의 미세형태학적 형질을 주사 전자현미경으로 관찰하여 화판 향축면, 배축면의 세포 외형, 배열, 수층벽, 큐티클의 미세형태를 자세히 기재하였으며, 분류학적, 계통학적 유용성을 검토하였다. 쉬땅나무족의 화판은 유두상 또는 원추형 유두상(PCS), 직사각형의 주름판상(TRS)로 확인되었으며, 향축면, 배축면의 화판 구조에 따라 크게 세 유형으로 구분하였다. Type I. PCS-TRS, Type II. PCS-PCS, Type III. TRS-TRS (향축면-배축면). 구분된 이들 유형과 수층벽, 큐티클 표면의 형태는 족 내 속간/종간 진단 형질로 유용하였을 뿐 아니라, 계통학적으로도 그 유용성이 확인되었다. 또한, 화판 표면의 세포의 미세형태와 수분매개자(곤충을 통한 충매)와의 연관성을 나타내는 추가적인 사례로 제시하였다.

• Journal of Plant Biotechnology
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• 제29권1호
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• pp.31-36
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• 2002

대두의 자엽 절편을 이용한 부정근 형성과 품종 반응을 비교하였다. 품종별 부정근 형성 양상은 직접적인 부정근 형성 품종과 callus형성이 동시에 이루어지는 품종들로 구분되었으며, callus 형성이 많은 품종에서는 부정근 형성수가 적었다. 자엽 절편에서의 부정근 형성은 절편의 향축면 치상시에만 부정근이 형성되었고, 배축면과 향축면이 동시에 치상될 경우에도 향축면에서만 부정근이 형성되며, 절편의 배축이 존재하였던 방향에서만 부정근이 형성되는 방향성이 존재하였다. 부정근 형성시 자엽 절편 내 탄수화물은 형성초기보다 형성이 가장 많은 유기 4일에 가장 많이 관찰되었으며 ,형성된 부정근의 생장이 이루어지는 7일 이후에는 관찰되지 않았다.

• 한국환경과학회지
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• 제11권11호
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• pp.1165-1172
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• 2002

The effects of salicylic acid(SA) and water deficit on growth and proline accumulation were investigated in cucumber(Cucurmis sativus L.) seedlings. Exogenous application of SA(100 $\mu$M-1 mM) led to a noticeable decrease in root and shoot growth, and dry weight of seedlings. Anatomical observation on leaf of cucumber revealed that the thickness of all leaf tissue components decreased in SA-treated plants. The effect was most pronounced on the width of the adaxial epidermis. In the separate effects of SA(0, 100, 500 and 1000 $\mu$M) and water deficit induced by PEG(0, 4.4, 7.0 and 9.6 %) on growth, the water deficit treatments had greater effects on growth traits than SA. Combinations of SA and PEG(SA+PEG) decreased shoot and root dry matter, and root length. Proline increased slightly in SA-treated seedlings, but exhibited a marked increase in water deficit application. Combinations of SA+PEG induced higher proline in both shoots and roots than SA stress alone. Shoots had higher proline than roots. Our data support a role of SA potentiating the osmotic stress response of germinating cucumber seedling.

• The Plant Pathology Journal
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• 제32권6호
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• pp.563-569
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• 2016

The spatial patterns for temperature distribution on crape myrtle leaves infested with sooty mold were investigated using a digital infrared thermal imaging camera. The mean temperatures of the control and sooty regions were $26.98^{\circ}C$ and $28.44^{\circ}C$, respectively. In the thermal images, the sooty regions appeared as distinct spots, indicating that the temperatures in these areas were higher than those in the control regions on the same leaves. This suggests that the sooty regions became warmer than their control regions on the adaxial leaf surface. Neither epidermal penetration nor cell wall dissolution by the fungus was observed on the adaxial leaf surface. It is likely that the high temperature of black leaves have an increased cooling load. To our knowledge, this is the first report on elevated temperatures in sooty regions, and the results show spatial heterogeneity in temperature distribution across the leaf surface.

• Applied Microscopy
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• 제44권4호
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• pp.111-116
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• 2014

This study investigates the developmental pattern of adhesive discs (ADs) to highlight the ontogeny and structural changes that occur during the growth of Boston ivy. Initiation to postmortem features of ADs were examined through light and scanning electron microscopy. The study also reveals a new finding of the dislocation of peripheral tissues of adaxial origin. Four phases of attachment are suggested with regards to its climbing behavior: 1) pre-attachment, 2) upon attachment, 3) after attachment, and 4) final attachment. During initiation, several ADs originate from tendril primordia without epidermal differentiation. However, different growth rates in the epidermis results in completely different ADs. ADs were discerned by size, shape, and color during expansion, but cells in the adaxial surface remained alive longer than the other side. Upon contact, the ADs demonstrate simultaneous growth and deterioration, but once attachment is established the latter process subdues to final stages. Epidermal transformation, adhesive secretion, cellular disruption, and mechanical stress were essential for the self-clinging nature of Boston ivy. The post-attachment sequence is also believed to be critical in achieving maximum mechanical strength to provide extensive support. The developmental process of ADs is prompted by tactile stimulation but in a highly organized and systematic manner.