• Title/Summary/Keyword: Activity stability

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Studies on Immobilized Alkaline Protease (고정화 Alkaline Protease 에 관한 연구)

  • 전문진;심상국;정동효
    • Microbiology and Biotechnology Letters
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    • v.6 no.1
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    • pp.33-40
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    • 1978
  • Immobilization of alkaline protease was investigated by absorbing the enzyme on adsorbents. Alkaline protease was adsorbed on silica gel selected as a carrier to immobilize the enzyme. In this study, properties of the immobilized enzyme were compared with those of the soluble enzyme. 1) The optimum pH (10.0) of the enzyme was not changed, but the activity was increased at alkaline pH by immobilization. 2) The optimum temperature of the immobilized enzyme was shifted from 50$^{\circ}C$ to 45$^{\circ}C$, while the temperature-activity Profile became broader than those of the soluble enzyme. 3) The pH stability of the immobilized enzyme was significantely increased at pH 4.0, althouth it did not change in the neutral and alkaline pH region. 4) The heat stability of the enzyme was enhanced in the temperature range of 55$^{\circ}C$∼65$^{\circ}C$ by the immobilization. 5) The immobilized enzyme retained 40% of its original activity after repetitive use for 6 times. 6) The enzyme stability was greately improved for a prolonged storage at 4$^{\circ}C$.

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Studies on the Stability of Hen′s Egg Yolk Immunoglobulins (난황 항체의 안정성에 관한 연구)

  • 이경애
    • Korean journal of food and cookery science
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    • v.12 no.1
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    • pp.54-59
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    • 1996
  • Immunoglobulins (IgY) were isolated from egg yolk of hens immunized with bovine serum albumin(BSA). The stability of anti-BSA IgY against heat and pH was investigated. Antibody activity was measured by enzyme linked immunosorbent assay. IgY was relatively heat-stable and most of the antibody activity remained after heating up 65$^{\circ}C$ for 30 minutes. IgY was stable at pH 5-11. However, inactivation of IgY was observed below pH 4, or above pH 12. Inactivation of IgY proceeded rapidly at low pHs(pH 2-3). Most of the antigen binding activity was lost at low pHs probably because of some conformational changes.

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An Analysis on the Management Situation of a Hansalim Consumer Cooperatives (A생협에 대한 경영성과 분석)

  • Kim, Ho
    • Korean Journal of Organic Agriculture
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    • v.28 no.1
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    • pp.59-68
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    • 2020
  • This study analyzed and suggested management situations and improving issues on a consumer cooperatives which has supplied environmentally friendly agricultural products from the year 2002. Indices of management analysis are stability ratio, activity ratio and profitability ratio. Management Stability ratio indices are debt ratio, net worth ratio, fixed ratio and current ratio. Management activity ratio ones include fixed assets turnover and net worth turnover. And profitability ratio is showed through return on investment, net return on sales and return on equity. In order to analyze these indices, financial statements after the closing entires are used each year.

Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven (키토산 섬유를 담체로 이용한 라이소자임 효소의 고정화)

  • Lee, So Hee
    • Textile Coloration and Finishing
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    • v.30 no.4
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    • pp.264-274
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    • 2018
  • Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA) was investigated. For this, 100 % chitosan non-woven was prepared as novel support for the enzyme immobilization. In addition, free lysozyme activity was examined depending on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal immobilization of lysozyme were pH 7.0, $25^{\circ}C$, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial activity at $4^{\circ}C$ over 30 days of storage. The lysozyme was immobilized effectively on chitosan non-woven by observation of surface morphology.

A Study on the Characteristics of Convective Activities related to Atmospheric Stability Index and Thunderstorms over the Naro Space Center (나로우주센터 상공의 대기 안정도지수 및 뇌운관련 대류활동 특성 연구)

  • Kim, Hong-Il;Choi, Eun-Ho;Seo, Seong-Gyu
    • Journal of Environmental Science International
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    • v.28 no.12
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    • pp.1133-1145
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    • 2019
  • Successful launch requires state-of-the-art launch vehicle technology and constant test operations, However, the meteorological threat to the launch vehicle flight trajectory is also an important factor for launch success. Atmospheric stability above the Naro Space Center at the this time is very important, especially because the initial flight operation can determine the success of the launch. Moreover, during the flight of launch vehicle with rapid pressure and thrust into the atmosphere, convection activity in the atmosphere may create environmental conditions that cause severe weather threats such as thunderstorms. Hence, studies of atmospheric instability characteristics over the Naro Space Center are a necessary part of successful launch missions. Therefore, the main aims of this study were to (1) verify the atmospheric stability index and convection activity characteristics over the Naro Space Center using radiosonde data observed from 2007 to 2018 by the Naro Space Center, (2) analyze changes in the atmospheric stability index according to monthly and seasonal changes, and (3) assess how the calculated atmospheric stability index is related to actual thunderstorm occurrence using statistical analysis. Additionally, we aimed to investigate the atmospheric characteristics above the Naro Space Center through the distribution chart of the atmospheric stability index during summer, when convection activity is highest. Finally, we assessed the relationship between lightning occurrence and unstable atmospheric conditions, through predictability analysis performed using the lightning observation data of the Korea Meteorological Administration.

Correlation between Balance and Lower Extremity Muscle Activity in Stroke Patients (뇌졸중 환자의 균형과 하지 근활성도와의 상관관계 연구)

  • Yang, Dae-Jung;Kim, Je-Ho;Jung, Yong-Sik;Uhm, Yo-Han;Park, Seung-Kyu
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.10 no.1
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    • pp.1-5
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    • 2012
  • Purpose : In this study, stroke patients' limits of stability and functional reach test and tibialis anterior, gastrocnemius muscle of lower extremity muscle activities to evaluate the correlation. Methods : 30 adult stroke patients to participate in this study. Limits of stability were measured using biorescue, tibialis anterior and gastrocnemius muscle of muscles activities were measured with functional reach test when there was movement. Results : Limits of stability and functional reach test (r=0.753, p<0.01), RMS value of the limits of stability and tibialis anterior muscle (r=0.706, p<0.01), RMS value of the limits of stability and gastrocnemius muscle (r=0.766, p<0.01), RMS value of the functional reach test and tibialis anterior muscle (r=0.835, p<0.01), RMS value of the functional reach test and gastrocnemius muscle (r=0.663, p<0.01), RMS value of the tibialis anterior and gastrocnemius muscle (r=0.816, p<0.01) correlations are shown as statistically significant. Conclusion : The balance and lower extremity muscle activities of stroke patients were studied, and were positively correlated with the RMS value of the limits of stability and functional reach test, tibialis anterior muscle, and gastrocnemius muscle. This study has shown that stroke patients' ankle joint muscle activity can greatly.

The Effects of Task Oriented Training with Suspension Device on Trunk Stability and Gross Motor Function of Children with Spastic Diplegia Cerebral Palsy (현수보조장치를 이용한 과제 지향적 훈련이 경직형 양하지 뇌성마비아동의 체간 안정성과 대동작기능에 미치는 영향)

  • Lee, Mi-Seon;Choi, Jong-Duk
    • Journal of the Korean Society of Physical Medicine
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    • v.8 no.4
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    • pp.637-645
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    • 2013
  • PURPOSE: The purpose of this study was to examine the effect of using a suspension device for arm reaching activity on trunk stability and gross motor function of children with spastic diplegia cerebral palsy. METHODS: The subject in this study consisted of 11, GMFCS(Gross Motor Function Classification System) III~IV children with spastic diplegia cerebral palsy, all of whom agreed to participate in the study. All subjects were divided into two groups: the experimental group using a suspension device, and the control group using no suspension device. For each group, a thirty-minute intervention was done twice per week during 8 weeks. Before and after intervention, each test was measured using TIS(Trunk Impairment Scale), GMFM (Gross Motor Function Measure) and PRT(Pediatric Reaching Test) to change trunk stability, gross motor function and arm reaching activity. The data were analyzed with the Wilcoxon signed rank test. RESULT: All two groups had a meaningful increase in GMFM-Sit data measured before and after intervention. The experimental group had a significant increase from an average of 78.83 to an average of 84.83 in GMFM-Crawling. For both groups, there was a substantial increase in the change in sitting position and arm reaching. CONCLUSION: According to the results of this study, the arm reaching activity using suspension device had an effect on trunk stability and gross motor function and it changed arm reaching activity.

The Relationship Between Color & Clothing Color Preference And Personality Traits Among High School Girls (여고생의 복색 선호와 성격특성과의 상관연구)

  • Im Nha Soo;Myeong Sook Han
    • Journal of the Korean Society of Clothing and Textiles
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    • v.10 no.1
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    • pp.37-45
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    • 1986
  • The purpose of this study was to investigate the relationship between color & clothing color preferences and three personality traits. Personality traits were measured with chung's Personality Scale. The questionare measuring Color & Clothing color preferences consisted of items designed to assess color preference, color repugnance clothing color preferences. These questionaire were administered to 302 senior high school girls in Seoul. Statistical analysis consisted of corelation and chi-square-test. The result were; 1) Activity, stability, sociability was no significant related to color preference. 2) Activity, sociability was positively related to color repugnance in Value but stability was no significant. 3) Sociability and stability was positively related to color preference, but activity and stability was no significant. 4) There was a positive relationship between color preference and clothing color preference. (r=0.8867) 5) The most preferred colors were white, light blue, and light green. The most repugnated colors were light red, dark red purple, and dark yellow. 6) The most preferred clothing colors were yellow, pink, and green in Spring, white and blue in Summer all kinds of colors, colors in Autumn, and Black and Grey in Winter.

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Effect of Electro-stimulation on Stability of Alcohol Dehydrogenase from Horse Liver (알콜 탈수소효소의 안정도에 미치는 전기 자극의 효과)

  • Lee Kang-Min;Kim Chunl-Sang;Lee Hye-Jung;Hahm Dae-Hyun;Park Chung-Ung
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.6
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    • pp.1723-1727
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    • 2004
  • We investigated the activity and stability of alcohol dehydrogenase from horse liver (HLADH) under the electric stimulation. The activity and stability of alcohol dehydrogenase depended on electric output voltaqe, stimulation time, pulse duration and pulse interval, and temperature. HLADH retained about 23% of its activity in buffer but 78% in 10% trehalose solution under electric stimulation with 10V, 10min, The stabilizing of enzymes against electric stimulation by stabilizing additives showed a great potential use of enzymes in biotechnology and medical engineering fields.

A Study on the Relationship between Food Habits, Food Preferences and Personality Traits of Children (아동의 식습관 및 식품기호와 성격특성과의 관계연구)

  • 이향자
    • Journal of the Korean Home Economics Association
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    • v.27 no.2
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    • pp.85-95
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    • 1989
  • To examine closely the relations between food habits, food preferences and personality traits, 141 boys and 146 girls in the 5th and the 6th grade of primary school in Incheon were studied by questionaires. The results of this study were as follows: 1. In food habit scores, girls got 2.28/3.00 and boys got 2.24/3.00 which meant boys and girls had the similar food habits. 2. The higher were their activity, sociability, responsibility, reflectiveness, and superiority, the higher score they showed in food habits. 3. Meats, fats and processed foods had much to do with emotional stability, and those who belonged to high group in emotional stability high preference for them. But girls who had high preference for meats were low in emotional stability. 4. Fishes of which bones we can eat, and potatoes had relation to activity, and those who belonged to high group in activity showed high preference for them. 5. Vegetables and processed foods had relation to sociability. Those who belonged to high group in sociability showed high preference for vegetables, but those who belonged to middle group in sociability showed highest preference for processed foods. 6. Meats and potatoes had much to do with responsibility. Those who belonged to low group in responsibility showed high preference for them. 7. Those who belonged to the high group in emotional stability, and superiority showed the high preferene for foods respectively, and superiority showed the high preference for foods respectively.

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