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Effect of Electro-stimulation on Stability of Alcohol Dehydrogenase from Horse Liver  

Lee Kang-Min (Division of Biology, College of Natural Science, Chonbuk National University)
Kim Chunl-Sang (Division of electronics, College of Engineering, Chonbuk National University)
Lee Hye-Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Hahm Dae-Hyun (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Park Chung-Ung (Division of Biology, College of Natural Science, Chonbuk National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1723-1727 More about this Journal
Abstract
We investigated the activity and stability of alcohol dehydrogenase from horse liver (HLADH) under the electric stimulation. The activity and stability of alcohol dehydrogenase depended on electric output voltaqe, stimulation time, pulse duration and pulse interval, and temperature. HLADH retained about 23% of its activity in buffer but 78% in 10% trehalose solution under electric stimulation with 10V, 10min, The stabilizing of enzymes against electric stimulation by stabilizing additives showed a great potential use of enzymes in biotechnology and medical engineering fields.
Keywords
Electro-stimulation; enzyme stability; alcohol dehydrogenase; horse liver;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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