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Antioxidant and Antibacterial Effects of Carnosic Acid on White Sausage (카르노스산에 의한 화이트소시지의 항산화 및 항균 효과)

  • Lee, Jo-Won;Choe, Il-Shin;Kim, Woan-Sub
    • Korean Journal of Organic Agriculture
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    • v.21 no.2
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    • pp.219-232
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    • 2013
  • The relevant main constituents of rosemary are compound of a vast number of polyphenolics, including carnosic acid, carnosol, rosmarinic acid, and ursolic acid. Recently, phenolic compounds of plant origin have attracted considerable attention due to their beneficial functional and nutritional effects including antioxidant and antibacterial activity. This study was carried out to investigated effect of carnosic acid on pH, thiobarbituric acid reactive substance (TBARS), volatile basic nitrogen (VBN), 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity, and total bacteria counts in white sausage during the storage at room temperature. Addition of carnosic acid was decreased of pH, TBARS, VBN, and enhanced antioxidant and antibacterial activity in white sausage. These activities increased with increasing concentrations of carnosic acid. Furthermore, the potent antibacterial activities of carnosic acid against pathogenic bacteria (Escherichia coli KCCM 11234 and Salmonella enteritidis KCCM 12021) were measured. Carnosic acid showed noticeable inhibitory effects on E. coli and S. enteritidis. In conclusion, carnosic acid might be used as a natural preservative in white sausage.

Screening for DPPH Free Radical Scavenging Activities of Autogenous Seaweeds in Jeju Island Using a Electron Spin Resonance (ESR) Spectroscopy (Electron Spin Resonance을 이용한 제주 자생 해조류의 DPPH Free Radical 소거활성 검색)

  • Cha, Seon-Heui;Heo, Soo-Jin;Jeon, You-Jin
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.4
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    • pp.252-259
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    • 2006
  • Extracts which were prepared by four different extractions - 80% methanol extracts (ME) at high ($70^{\circ}C$) and a room temperature ($20^{\circ}C$), respectively and aqueous extracts (AE) at both temperatures with the residue after the methanol extracts - of 10 green, 19 brown and 25 red seaweeds collected in Jeju Island coast were examined for their DPPH free radical scavenging activity using a ESR (electron spin resonance) spectroscopy. A variety of the extracts showed positive scavenging effect against DPPH free radical (except the green seaweeds). Among the extracts, the brown seaweed extracts exhibited the highest scavenging activity. Especially, Sargassum spp. of the brown seaweeds have remarkable scavenging activities - both methanolic and aqueous at the both temperatures ($20^{\circ}C$ and $70^{\circ}C$). On the other hand, ME showed better scavenging activity than AE in the red seaweed extracts. These results indicate that autogenous seaweeds in Jeju will be potential natural antioxidants for functional food compounds.

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Antimicrobial Activity and Food Storage of LDPE Silica Film Containing Antimicrodial Compounds (항균성물질이 함유된 Silica LDPE필름의 항균효과 및 식품 저장성)

  • 김현수;성림식;이인선
    • KSBB Journal
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    • v.17 no.4
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    • pp.350-356
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    • 2002
  • Low density polyethylene(LDPE) film was fabricated with the addition of benzoic acid, or/and JP which contained water-soluble silica adsorbed a natural antimicrobial compounds. The used antimicrobial compounds were isolated from culture broths of methylotropic actinomycetes strains MO-16 and MO-17 which was newly isolated from soils. An antimicrobial compound retained antimicrobial activity after heat treatment at 121$^{\circ}C$ for 5 min. JP, which obtained from Japan as a antimicrobial agent, showed antimicrobial activity in the concentration of 100 mg/mL. The silica LDPE film revealed the growth inhibition of total aerobic bacteria in packaged minced pork compared with commercial film and of E. coil on a contained agar plate. In the storage testing of various packaged foods at room temperature for 30 days, the film showed excellent preservation compared with commercial film in case of small tomato and Agaricus bisporus.

Antifungal Activity of Anemarrhena asphodeloides, Coptis japonica and Phellodendron amurense Extracts against Phytophthora Blight (지모, 일황련 및 황백나무 추출액의 항균활성)

  • EunSooDoh
    • Korean Journal of Plant Resources
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    • v.10 no.4
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    • pp.351-359
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    • 1997
  • Antifungal activities of the crude extracts of Anemarrhena asphodeloides. Coptis japonica and Phellodendron amurense were tested against Phvtophthora capsici. and the control effect on red-pepper phytophthora hlight and phytotoxicities of red-pepper were investigated. The results were summarized as follows; Mycelial growth and zoosporangial germination of the red-peppcr phytophthora hlight organism P. capsici were inhihited hy thc crude extracts of plant materials. Methanol extracts or plant materials had hctter antifungal activity than water extracts at hoth a room temperature and a hoiling condition. Antifungal activities of three crude extracts were gradually decreased with prolonged storage period. Red-pepper phytophthora hlight was effectively controlled hy the crude extracts of three plant materials. Of these. the crude extract of C. japonica was marvelously effective. Phytotoxic symptom to red-pepper seedling showed hy water cultural method hut not by pot test. Seed germination and radicle growth of red-pepper were inhihited hy the crude extracts of three plant materials. Phytotoxic symptoms in the leaves and fruits of red-pepper were not ohserved with exogenous foliage application of the three crude extracts.

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Preparation and Antimicrobial of Zinc Calcium Alginate Films according to Concentration (농도에 따른 알긴산 아연칼슘 필름의 제조 및 항균성)

  • Seo, Hye-Jin;Jun, So-Yoon;Lee, Woo-Seung;Park, Jae-Hoon;Son, Tae-Won
    • Polymer(Korea)
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    • v.38 no.5
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    • pp.580-587
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    • 2014
  • Composite films of zinc calcium alginate were prepared by a film maker from 7 wt% sodium alginate solution and then they solidified into 3, 5 wt% content $ZnCl_2$, $CaCl_2$ solution followed by washing and drying at room temperature. The characteristics were measured with several methods (antimicrobial activity, water solubility, swelling ratio and viscosity, SEM, EDS) and the film properties were investigated. Composite films of zinc calcium alginate showed an increase in the water resistance by increasing $ZnCl_2$ and $CaCl_2$ content and the antimicrobial test showed that the calcium alginate as well as zinc alginate films result in excellent antimicrobial activity in the two strains, Staphylococcus and Escherichia coli. The results show the possible improvement of the physical properties of composite films.

Reevaluation of Photon Activation Yields of 11C, 13N, and 15O for the Estimation of Activity in Gas and Water Induced by the Operation of Electron Accelerators for Medical Use

  • Masumoto, Kazuyoshi;Matsumura, Hiroshi;Kosako, Kazuaki;Bessho, Kotaro;Toyoda, Akihiro
    • Journal of Radiation Protection and Research
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    • v.41 no.3
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    • pp.286-290
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    • 2016
  • Background: Activation of air and water in the electron linear accelerator for medical use has not been considered severely. By the new Japanese regulation for protection of radiation hazard, it became indispensable to evaluate of activation of air and water in the accelerator room. The measurement of induced activity in air and water components in the electron energy region of 10 to 20 MeV is very difficult, because this energy region is close to the threshold energy region of photonuclear reactions. Then, we measured the photonuclear reaction yields of $^{13}N$, $^{15}O$, and $^{11}C$ by using the electron linear accelerator. Obtained data were compared with the data calculated by the Monte Carlo method. Materials and Methods: An activation experiment was performed at the Research Center for Electron Photon Science, Tohoku University. Highly purified $SiO_2$, $Si_3N_4$, and carbon disks were irradiated for 10 minutes by bremsstrahlung converted by a tungsten plate. Induced activity from C, N, and O was obtained. Monte Carlo calculation was performed using MCNP5 and AERY (DCHAIN-SP) to simulate the experimental condition. Cross section data were adopted the KAERI dataset. Results and Discussion: In our experiment in hospital, calculated values were not agreed with experimental values. It might be three possible reasons as the cause of this deference, such as irradiation energy, calculation procedure and cross section data. Obtained data of this work, calculated and experimental values were good agreement with each other within one order. In this work, we used KAERI dataset of photonuclear reaction instead of JENDL. Therefore, it was found that the photonuclear cross section data of light elements are most important for yield calculation in these reactions. Conclusion: Further improvement for calculation using a new dataset JENDL/PD-2015 and considering electron energy spreading will be needed.

Antimicrobial Activity and Food Storage of LDPE Ceramic Film Containing Antimicrobial Agents (항균성물질이 함유된 세라믹 LDPE필름의 항균효과 및 식품의 저장성)

  • 김현수;성림식;유대식
    • KSBB Journal
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    • v.15 no.6
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    • pp.600-604
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    • 2000
  • Low density polyethylene(LDPE) film was fabricated with the addition of synthetic ceramic which contained a natural antimicrobial agents. The antimicrobial agents used were isolated from culture broths of methylotropic actinomycetes strains MO-16 and MO-17, and Streptomyces sp. No. 31, which was newly isolated from soils as an antifungal agent. Four-day old culture broth of Streptomyces sp. No. 31 showed strong antifungal activity against Aspergilus niger, a test strain, and retained antimicrobial activity after heat treatment at $121^{\circ}C$ for 15 min. The ceramic LDPE film reduced the growth of total aerobic bacteria in packaged minced pork compared with commercial film. The film revealed a 40 to 50% growth inhibition of E. coli on a contained agar plate. In the storage testing of various packaged foods at room temperature for 30 days, the ceramic LDPE film showed excellent preservation compared with commercial film.

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Studies on the Utilization of Arkshell 1. Preparation and Quality Stability during Storage of Powdered Dried Arkshell for Instant Soup (피조개의 이용에 관한 연구 1. 피조개 분말수우프의 제조 및 저장중의 품질안정성)

  • Kim, Hee-Yun
    • Journal of Food Hygiene and Safety
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    • v.3 no.4
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    • pp.217-223
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    • 1988
  • For the Effective utilization of the fish resources in coastal regions, investigations on preparation of powdered dried arkshell instant soup., quality stability of the products during storage and utilization as a food material were carried out with arkshell, Anadara broughtonii. Three kinds of powdered instant soup were prespared as 0% table salt(A), 5% table salt (B), 15% table salt(C) and packed with vacuum in laminated film bag. (polyester/nylon: 85${mu}ell$/15${mu}ell$, 18$\times$27cm) Their processing conditions and quality stability during storage at room temperature for 90 days were examined. Powdered instant soup was made by washing raw arkshell to remove visceral, clay, sand and blood, hot air drying(60$\pm$1$^{\circ}C$, 20 hrs) after draining and pulverizing dried arkshell to 35 mesh. Powdered instant soup was made by adding 2% sugar 0% table salt (5% and 15% table salt), 10.5% monosodium glutamate, 0.3% black pepper and 0.3% garlic powder to the pulverized dried arkshell. The condition of moisture and water activity of the products were 5.9-6.9% and 0.42-0.43, respectively. The moisture content s , water activity and pH of the products were showed little change and volatile basic nitrogen of them increased slightly during storage. Thiobarbituric acid value increased up to 60 days of storage and then decreased slightly. In solubility, powdered instant soup were showed no remarkable difference comparing with goods on the market. The color value of th products were showed little change during storage, In sensory evaluation, product B were scored slightly higher, in most cased, in flaver, color. taste and overall acceptability comparing with product A or product C during storage. Judiging form the sensory evaluation, powdered instant soup of 5% table salt (B) were the most desirable, and the quality of the products was stable for 90 days at 20$\pm$3$^{\circ}C$.

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Changes in th Chemical Components and Antioxidant Activity of Strawberry by Slow-released ClO2 Gas Gel-Pack during Storage (서방형 이산화염소 가스 젤팩을 이용한 딸기저장 중 주요 성분 및 항산화 활성의 변화)

  • Lee, Kyung-Haeng;Yoon, Ye-Ji;Lee, Bom;Bong, So-Jung;Kim, Hyun-Kyoung;Kwak, Il-Hwan;Kim, Hong-Gil
    • The Korean Journal of Food And Nutrition
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    • v.30 no.4
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    • pp.823-829
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    • 2017
  • To increase the shelf-life of strawberry, samples were treated with two gel packs containing slow-released chlorine dioxide ($ClO_2$) gas at 3~5 ppm for 6 days at room temperature and the changes in the major chemical components (ascorbic acid, polyphenols, flavonoids and anthocyanin) contents and antioxidative activities (DPPH, ABTS radical scavenging and metal chelating activity) were investigated. The content of ascorbic acid of control was 40.38 mg% and contained 35.67~44.75 mg% during 6 days. There was no tendency to increase or decrease during storage period. The contents of ascorbic acid of control and 3~5 ppm $ClO_2$ gas treated samples were not significantly different during storage period. The content of polyphenol compounds of initial stage was 111.23 mg% and contained 117.78~132.40 mg% during 6 days. The contents of polyphenol compounds of 3~5 ppm $ClO_2$ gas treated samples were 103.51~130.25 mg%. There were no significant different between them during storage. The flavonoids and anthocyanin contents were not different from the control during storage period regardless of 3~5 ppm $ClO_2$ gas treatment. Furthermore, antioxidative activities were not different among the control and $ClO_2$ gas treatments during storage.

Preparation of Bi/Bi2MoO6 Plasmonic Photocatalyst with High Photocatalytic Activity Under Visible Light Irradiation

  • Zou, Chentao;Yang, Zhiyuan;Liang, Mengjun;He, Yunpeng;Yang, Yun;Yang, Shuijin
    • Nano
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    • v.13 no.11
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    • pp.1850127.1-1850127.13
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    • 2018
  • Bi metal deposited on $Bi_2MoO_6$ composite photocatalysts have been successfully synthesized via a simple reduction method at room temperature with using $NaBH_4$ as the reducing agent. The photocatalytic activity of the composite was evaluated by degradation of rhodamine B (RhB) and bisphenol A (BPA) solution under visible light. The rate constant of $Bi/Bi_2MoO_6$ composite to RhB is 10.8 times that of $Bi_2MoO_6$, and the degradation rate constant of BPA is 6.9 times of that of $Bi_2MoO_6$. Nitrogen absorption-desorption isotherm proved that the increase of specific surface area is one of the reasons for the improvement of photocatalytic degradation activity of $Bi/Bi_2MoO_6$ composites. The higher charge transfer efficiency of $Bi/Bi_2MoO_6$ is found through the characterization of the photocurrent and impedance, which are attributed to the surface plasmon resonance (SPR) effect produced by the introduction of the metal Bi monomer in the composite. Free radical capture experiments proved that cavitation is the main active species. Based on the above conclusions, a possible mechanism of photocatalytic degradation is proposed.