• BMB Reports
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• v.28 no.6
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• pp.484-489
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• 1995

The product of bacteriophage T7 gene 2.5 is a single-stranded DNA binding protein and plays an important role in T7 DNA replication, recombination, and repair. Genetic analysis of T7 phage defective in gene 2.5 shows that the gene 2.5 protein is essential for T7 DNA replication and growth (Kim and Richardson, 1993). The C-terminal truncated gene 2.5 protein ($GP2.5-{\Delta}21C$) cannot substitute for wild-type gene 2.5 protein in vivo; suggesting that the C-terminal domain of gene 2.5 protein is essential for protein-protein interactions (Kim and Richardson, 1994; J. Biol. Chem. 269, 5070-5078). Truncated gene 2.5 proteins lacking 19 residues ($GP2.5-{\Delta}19N$) and 39 residues ($GP2.5-{\Delta}39N$) from the amino-terminal domain were constructed by in vitro mutagenesis. $GP2.5-{\Delta}19N$ can support the growth of T7 phage lacking gene 2.5 while $GP2.5-{\Delta}39N$ cannot substitute for wild-type gene 2.5 protein in vivo; however, its ability to bind to single-stranded DNA is not affected. These results clearly demonstrate that the 20~39 amino-terminal region of gene 2.5 protein is required for T7 growth in vivo but may not be involved in DNA binding activity.

• Fashion & Textile Research Journal
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• v.7 no.5
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• pp.535-541
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• 2005

The health of feet is connected with individual's health and affects a man's activity. Shoes need to be designed to protect feet and to absorb the impact of land. In order to choose suitable shoes for feet, the foot size and shape must be considered, so it is essential to grasp the exact size and shape of the foot. This study aims to present fundamental data on shoes' easy order prototype development for choosing shoes of good wearing comfort, by classifying feet size and shape junior high school boys in the early adolescent period. The subject were 234 Korean junior high school boys age from 14 to 16 years old. The subjects were directly measured anthropometrically and indirectly analyzed photographically. 6 factors were extracted through factor analysis and those factors comprised 79.42% of total variance. The factors characterizing foot girth and width, foot length, foot height, foot shape around the fifth toes, angle of foot breadth and foot shape around the first toes. 3 clusters as their foot shape were categorized using 6 factor scores by cluster analysis. Type 1 was characterized by long large foot with deformed first toe. Type 2 had smaller in foot girth, width and length than other types and with deformed fifth toe. Type 3 had average size and high foot shape. Shoes prototype which is to be developed later on will be able to generate 2D flattening in the foot sole form. Therefore, it would be a great support in producing and choosing appropriate shoes if forms are classified by subdividing foot form classification and extract a factor which shows only the foot sole shape.

• Fashion & Textile Research Journal
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• v.9 no.3
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• pp.319-326
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• 2007

The health of feet is connected with individual's health and affects a man's activity. Shoes need to be designed to protect feet and to absorb the impact of land. In order to choose suitable shoes for feet, the foot size and shape must be considered, so it is essential to grasp the exact size and shape of the foot. This study aims to present fundamental data on shoes' easy order prototype development for choosing shoes of good wearing comfort, by classifying feet size and shape junior high school boys in the early adolescent period. The subject were 217 Korean junior high school girls in age from 14 to 16 years old. The subjects were directly measured anthropometrically and indirectly analyzed photographically. 7 factors were extracted through factor analysis and those factors comprised 78.59% of total variance. The factors were characterized foot length, foot girth and width, foot shape around the fifth toes, foot shape around the first toes, angle of foot breadth, foot height, and foot length of upper foot. 3 clusters as their foot shape were categorized using 7 factor scores by cluster analysis. Type 1 had smaller in foot girth, width and length than other types and with deformed fifth toe. Type 2 had average size and high foot shape. Type 3 was characterized by long large foot with deformed first toe. The results would be a great support in producing and choosing appropriate shoes if forms are classified by subdividing foot form classification and extract a factor which shows only the foot sole shape.

• Journal of Korea Port Economic Association
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• v.26 no.2
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• pp.99-118
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• 2010

This objective of this study is to thoroughly analyze the policies of international organizations and major advanced countries relevant to the realization of Green port To construct Green ports, there first must be competition to build such ports in sustainable, fixed quantities. Second, there is a great need for cooperation and support networks made binding by mutual agreements on ship recycling. Third, there is a need for scientific research on responses to changes in environmental regulations and on environmental issues. Today, the majority of the world's ports use gasoline or electric energy, and improving capacities for self-sufficiency through development of new and renewable energy is judged to be a pressing task. The conditions for an eco-friendly port is that it must be an important center for economic and industrial activity, and valuable as a site where people live and work, with residences and work places existing in close proximity.

• International Journal of Oral Biology
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• v.30 no.3
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• pp.85-90
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• 2005

Homeostatic pH is very important for various cellular processes, including metabolism, survival, and death. An imbalanced-pH might induce cellular acidosis, which is involved in many abnormal events such as apoptosis and malignancy. One of several factors contributing to the onset of metabolic acidosis is the production of lactate and protons by lactate dehydrogenase (LDH) in anaerobic glycolysis. LDH is an important enzyme that catalyzes the reversible conversion of pyruvate to lactate. This study sought to examine whether decreases in extracellular pH induce apoptosis of CHO cells, and to elucidate the role of mitogen-activated protein kinases (MAPKs) in acidification-induced apoptosis. To test apoptotic signaling by acidification we used CHO dhfr cells that were sensitive to acidification, and CHO/anti-LDH cells that are resistant to acidification-induced apoptosis and have reduced LDH activity by stable LDH antisense mRNA expression. In the present study, cellular lactic acid-induced acidification and the role of MAPKs signaling in acidification-induced apoptosis were investigated. Acidification, which is caused by $HCO{_3}^-$-free conditions, induced apoptosis and MAPKs (ERK, JNK, and p38) activation. However, MAPKs were slightly activated in acidic conditions in the CHO/anti-LDH cells, indicating that lactic acid-induced acidification induces activation of MAPKs. Treatment with a p38 inhibitor, PD169316, increased acidification-induced apoptosis but apoptosis was not affected by inhibitors for ERK (U0126) or JNK (SP600125). Thus, these data support the hypothesis that activation of the p38 MAPK during acidification-induced apoptosis contributes to cell survival.

• International Journal of Oral Biology
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• v.39 no.2
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• pp.65-73
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• 2014

Chios Gum Mastic (CGM) is a natural resin extracted from the leaves of Pistacia lentiscus, a plant endemic to the Greek island of Chios. It has been used by traditional healers, and it has antibacterial, antifungal properties, and therapeutic benefits for the skin. The CGM reduces the formation of dental plaque and bacterial growth in oral saliva, and recent studies have demonstrated the role of antioxidant activity of CGM. Although CGM has been widely investigated, its protective effect against oxidative-damage to keratinocytes, as well as the relationship between CGM and autophagy, has not been investigated. The aim of this study was to assess the protective effect of CGM against $H_2O_2$-induced oxidative stress and to evaluate the autophagic features induced by CGM in human keratinocytes. The pretreatment with CGM significantly reduced apoptosis in $H_2O_2$-exposed HaCaT cells. It promoted the degradation of caspase-3, caspase-8, and caspase-9; and it induced the formation of the processed PARP. The treatment with CGM caused an increase in vesicle formation compared to control group. The level of p62 was reduced and the conversion of LC3-I to LC3-II was increased in CGM treated HaCaT cells. Also, the treatment with CGM increased cleavage of ATG5-ATG12 complex. In summary, CGM helps the cells to survive under stressful conditions by preventing apoptosis and enhancing autophagy. Besides, the present investigation provides evidence to support the antioxidant potential of CGM in vitro and opens up a new horizon for future experiments.

• Journal of Digital Convergence
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• v.15 no.2
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• pp.233-238
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• 2017

The purpose of this study is to investigate the necessity of policy approach in terms of complex combination of tourists and fishing villages. In order to achieve the purpose of this study, the policies related to the marine tourism industry were investigated and the laws related to the development of the coastal area and fishing port area were investigated. As a convergence approach to marine tourism policy, First, the development of marine tourism resources should be based on the increase of hydrophilic activities and the characteristics of the four seasons. In particular, the need to dig through support of projects such as the successful model that made aquarium associations etc. that are currently in progress, such as "fishing village experience".

• Korean Journal of Childcare and Education
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• v.9 no.3
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• pp.291-308
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• 2013

This study focuses on variables affecting role performance of infant care teachers and aims at developing quality care service by means of role performance of infant care teachers. The research participants were 350 infant care teachers in nurseries in Seoul, Incheon and Gyeonggi area. The results are as follows. First, there was no difference by age on teachers' emotional intelligence, organizational culture and role performance. As for educational level, there was a difference in 'counseling and emotional support' a subordinated factor in role performance. As for career, there was a difference in 'education and teaching activity' a subordinate factor of role performance. As for working facility type, there was a difference in emotional intelligence and organizational culture. Second, there was correlation between infant care teachers' emotional intelligence and organizational culture, and their role performance. Third, infant care teachers' emotional intelligence was the best predictor of their role performance. Therefore, more studies should be done to raise the emotional intelligence of infant care teachers.

• Speech Sciences
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• v.1
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• pp.153-200
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• 1997

In languages such as Japanese, it is very common to observe that short peripheral vowel are completely voiceless when surrounded by voiceless consonants. This phenomenon has been known as Montreal French, Shanghai Chinese, Greek, and Korean. Traditionally this phenomenon has been described as a phonological rule that either categorically deletes the vowel or changes the [+voice] feature of the vowel to [-voice]. This analysis was supported by Sawashima (1971) and Hirose (1971)'s observation that there are two distinct EMG patterns for voiced and devoiced vowel in Japanese. Close examination of the phonetic evidence based on acoustic data, however, shows that these phonological characterizations are not tenable (Jun & Beckman 1993, 1994). In this paper, we examined the vowel devoicing phenomenon in Korean using data from ENG fiberscopic and acoustic recorders of 100 sentences produced by one Korean speaker. The results show that there is variability in the 'degree of devoicing' in both acoustic and EMG signals, and in the patterns of glottal closing and opening across different devoiced tokens. There seems to be no categorical difference between devoiced and voiced tokens, for either EMG activity events or glottal patterns. All of these observations support the notion that vowel devoicing in Korean can not be described as the result of the application of a phonological rule. Rather, devoicing seems to be a highly variable 'phonetic' process, a more or less subtle variation in the specification of such phonetic metrics as degree and timing of glottal opening, or of associated subglottal pressure or intra-oral airflow associated with concurrent tone and stricture specifications. Some of token-pair comparisons are amenable to an explanation in terms of gestural overlap and undershoot. However, the effect of gestural timing on vocal fold state seems to be a highly nonlinear function of the interaction among specifications for the relative timing of glottal adduction and abduction gestures, of the amplitudes of the overlapped gestures, of aerodynamic conditions created by concurrent oral tonal gestures, and so on. In summary, to understand devoicing, it will be necessary to examine its effect on phonetic representation of events in many parts of the vocal tracts, and at many stages of the speech chain between the motor intent and the acoustic signal that reaches the hearer's ear.

• The Journal of Advanced Prosthodontics
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• v.1 no.1
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• pp.56-61
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• 2009

STATEMENT OF PROBLEM. The success of titanium implants is due to osseointegration or the direct contact of the implant surface and bone without a fibrous connective tissue interface. PURPOSE. The purpose of this study was to evaluate the osteoblast precursor response to titanium-10 tantalum-10 niobium(Ti-Ta-Nb) alloy and its sputtered coating. MATERIAL AND METHODS. Ti-Ta-Nb coatings were sputtered onto the Ti-Ta-Nb disks. Ti6-Al-4V alloy disks were used as controls. An osteoblast precursor cell line, were used to evaluate the cell responses to the 3 groups. Cell attachment was measured using coulter counter and the cell morphology during attachment period was observed using fluorescent microscopy. Cell culture was performed at 4, 8, 12 and 16 days. RESULTS. The sputtered Ti-Ta-Nb coatings consisted of dense nanoscale grains in the range of 30 to 100 nm with alpha-Ti crystal structure. The Ti-Ta-Nb disks and its sputtered nanoscale coatings exhibited greater hydrophilicity and rougher surfaces compared to the Ti-6Al-4V disks. The sputtered nanoscale Ti-Ta-Nb coatings exhibited significantly greater cell attachment compared to Ti-6Al-4V and Ti-Ta-Nb disks. Nanoscale Ti-Ta-Nb coatings exhibited significantly greater ALP specific activity and total protein production compared to the other 2 groups CONCLUSIONS. It was concluded that nanoscale Ti-Ta-Nb coatings enhance cell adhesion. In addition, Ti-Ta-Nb alloy and its nanoscale coatings enhanced osteoblast differentiation, but did not support osteoblast precursor proliferation compared to Ti-6Al-4V. These results indicate that the new developed Ti-Ta-Nb alloy and its nanoscale Ti-Ta-Nb coatings may be useful as an implant material.