• Asian-Australasian Journal of Animal Sciences
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• 제26권10호
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• pp.1406-1415
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• 2013

The study was conducted to correlate the periparturient immune status in terms of neutrophil functions and cytokine expression in peripheral blood mononuclear cell culture with impending postpartum reproductive disorders in buffaloes. Forty pregnant buffaloes were observed for occurrence of postpartum reproductive disorders (PRD), i.e., metritis, endometritis and delayed uterine involution etc., during one week prepartum to four weeks postpartum period. A representative number (n = 6) of buffaloes that did not develop any PRD were included in group I (healthy, control), while the animals which experienced PRD were assigned into group II (PRD, n = 8). The blood samples were collected at weekly interval from one week prepartum to four weeks postpartum period considering the day of calving as 'd 0'. Differential leucocytes counts, superoxide and hydrogen peroxide production activity in isolated neutrophils and the mRNA expression profile of cytokines i.e., IL-2, IL-4 and IFN-${\gamma}$ in PBMC culture were studied in all the samples. A higher total leucocytes, neutrophil and band cells count along with impaired neutrophil functions i.e., lowered level of production of superoxide and hydrogen peroxide before parturition and during early postpartum period were observed in buffaloes developing PRD. Further, a lower expression of IL-2, IFN-${\gamma}$ and IL-4 mRNA in PBMC culture was observed at calving in buffaloes that subsequently developed PRD at later postpartum. Thus, suppression in neutrophil function and cytokine expression at prepartum to early postpartum period predisposes the buffaloes to develop postpartum reproductive disorders. Hence, monitoring of neutrophils function and cytokine expression profile would be effective to predict certain reproductive disorders at late pregnancy or immediately after parturition in buffaloes. In future, this may be a novel approach for determining suitable management and therapeutic decisions for prevention of commonly occurring reproductive disorders in farm animals.

• 한국수자원학회논문집
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• 제41권8호
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• pp.785-795
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• 2008

너울은 항만활동 및 선박운행을 방해하며, 연안지역의 인명사고와 지형 변화 등을 야기키는 주요 원인이다. 이러한 너울의 관측 및 예측은 장기간에 걸친 파랑 및 바람 관측자료와 주변외해 기상자료의 거시적인 검토가 필요하기 때문에 자료 수집 및 분석에 많은 시간과 노력을 필요로 한다. 이러한 어려움 때문에 지금까지는 너울에 관한 연구가 활발히 이루어지기 어려웠다. 이에 본 연구는 인터넷 기반의 실시간 모니터링 시스템인 필드박스를 개발하고 이로부터 수집한 파랑자료, 선형파이론과 국내 기상청과 NASA의 기상자료 등을 이용하여 우리나라에서 발생하는 너울의 특성과 원인을 분석하였다. 2004년부터 2006년 동안 광안타워에서 관측된 자료를 이용하여 너울의 빈도와 총발생시간을 월별로 통계 분석하였다. 따라서 우리나라의 분기별 너울 발생의 특징을 규명할 수 있었으며 국내에 발생하는 너울의 원인과 위치적 특징 등을 도출하였다.

• 한국지리정보학회지
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• 제11권2호
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• pp.28-37
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• 2008

본 연구에서는 원격탐사자료를 이용하여 현장관측이 불가능한 북한 지역에 대한 NDVI (Normalized Difference Vegetation Index) 산출을 통하여 1999년부터 2005년까지 7년간의 지표 상태 모니터링 하였다. 북한의 산림 지역은 소위 한반도의 폐라고 불리며, 한반도 내에서 가장 풍부한 산림지역이 위치하고 있다. NDVI는 식생의 활력 지수를 나타내며, 유사한 많은 연구에서 사용되었다. 본 연구에서는 북한 지역 식생의 변화를 탐지하고 그 원인을 분석하였다. NDVI의 변동은 북한 지역에서 가뭄이나 태풍의 영향을 잘 반영하고 있으며, 북한 지역이 태풍이나 가뭄을 겪고 있을 때 평년보다 낮은 NDVI 수치가 나타났다. 특히, 최근 정치적, 경제적으로 큰 이슈가 되고 있는 북한 지역 식량 생산 정보이 간접적인 파악을 위해 본 연구에서 분석한 결과를 토대로 식량 생산 동향을 유추하였다.

• Biomolecules & Therapeutics
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• 제25권6호
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• pp.618-624
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• 2017

Betulinic acid (BA), a natural pentacyclic triterpene found in many medicinal plants is known to have various biological activity including tumor suppression and anti-inflammatory effects. In this study, the cell-death induction effect of BA was investigated in BV-2 microglia cells. BA was cytotoxic to BV-2 cells with $IC_{50}$ of approximately $2.0{\mu}M$. Treatment of BA resulted in a dose-dependent chromosomal DNA degradation, suggesting that these cells underwent apoptosis. Flow cytometric analysis further confirmed that BA-treated BV-2 cells showed hypodiploid DNA content. BA treatment triggered apoptosis by decreasing Bcl-2 levels, activation of capase-3 protease and cleavage of PARP. In addition, BA treatment induced the accumulation of p62 and the increase in conversion of LC3-I to LC3-II, which are important autophagic flux monitoring markers. The increase in LC3-II indicates that BA treatment induced autophagosome formation, however, accumulation of p62 represents that the downstream autophagy pathway is blocked. It is demonstrated that BA induced cell death of BV-2 cells by inducing apoptosis and inhibiting autophagic flux. These data may provide important new information towards understanding the mechanisms by which BA induce cell death in microglia BV-2 cells.

• 인터넷정보학회논문지
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• 제3권3호
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• pp.11-17
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• 2002

웹을 통한 원격교육은 시간과 공간의 문제를 극복할 수 있다는 큰 장점에도 불구하고 강의실 및 집합교육에 비해 연수생 관리가 취약하다는 문제점을 지니고 있다. 이러한 문제는 원격교육활동의 성취수준 및 목표도달에 대한 커다란 장애로 부담이 되고 있고 원격교육담당자에게 많은 고민을 안겨주고 있다. 원격교육담당자는 원격교육활동에 대한 연수생의 참여와 반응을 계속적으로 모니터링 해야 하고 이에 대한 적절한 피드백을 연수생에게 제공하여야 함에도 불구하고 기존의 원격교육시스템은 교수학습활동에 중점을 둔 나머지 원격교육활동에 대한 효율적 관리기능을 제공하지 못하고 있다. 따라서 본 논문에서는 원격교육의 일환으로 실시되고 있는 원격교원연수의 효율적 운영이 가능하며 교원연수가 목표로 하는 성취수준의 효과적 달성을 위해 원격교원연수의 적합한 관리요소를 추출하고 이를 시스템 또는 연수 관리자가 연수과정에 적용하여 연수생에 대한 적절한 피드백의 제공이 가능한 원격교원연수 관리시스템을 효율적으로 구현하였으며 교육과정과 컴퓨팅 환경에 따라 시스템의 유연한 변경도 가능하게 되었다.

• 한국컴퓨터정보학회논문지
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• 제12권2호
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• pp.151-159
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• 2007

반도체 제조 과정에서 생산과 관련된 모든 현상 데이터를 수집하는 것은 매우 중요한 작업이다. 수집된 자료의 분석을 통해 장비의 가동률, 고장 진단, 공정 제어 및 예측되는 장애 요소 제거 등에 활용할 수 있으며, 이는 궁극적으로 생산 효율 향상에 기여할 수 있기 때문이다. 많은 장비제조업체들이 이러한 목적으로 EES (Equipment Engineering System)을 도입했으며, 최근에는 웹 사용이 일상화되면서 HTTP/SOAP 프로토콜을 활용하여 장비 모니터링의 범위를 확대하는 방안이 제안되고 있다. 이러한 웹 기반 EES를 실현하기 위해서는 여러 반도체 장비에서 생성되는 다양한 메시지의 형식을 통합, 표준화함으로써 EDA(Equipment Data Aquisition)를 용이하게 하는 작업이 선행되어야 한다. 본 논문에서는 반도체 장비 간 통신에 사용되는 다양한 프로토콜의 분석을 통하여 상이한 형식의 정보를 통합하기 위한 방안을 제안하고 이를 지원하기 위한 소프트웨어 구조를 설계한다.

• The Journal of Korean Physical Therapy
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• 제21권2호
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• pp.109-116
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• 2009

Purpose: $17\beta$-estradiol is the most active endogenous estrogen, which is related to favorable changes in the plasma lipid profile, to relaxation of the coronary vessels, and to a decrease in platelet aggregation and vascular smooth muscle cell migration. However, although the beneficial effect of estrogens on plasma lipoproteins (ie, lowering low-density lipoprotein and increasing high-density lipoprotein cholesterol) contributes to cardiovascular protection, it does not fully account for the protective effect, particularly in the application of physical therapy, including low frequency electrical stimulation. Methods: The aim of this study was to demonstrate the inhibition of stressors, such as endothelin-1 (ET-1), serotonin (5-hydroxytryptamine, 5-HT), prostaglandin $F2\alpha$ ($PGF2\alpha$), and a protein kinase C (PKC) activator 12-deoxyphorbol 13-isobutyrate (DPB), induced isometric tension by $17\beta$-estradiol in vascular smooth muscle strips, respectively. In addition, the effects of low frequency electrical stimulation at the meridian points (CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52) on the indirect antihypertensive effect were examined by monitoring the changes in the serum $17\beta$-estradiol concentration in healthy volunteers. Results: Isometric tension analysis showed that the responses of inhibited tension by $17\beta$-estradiol were similar to the same stressors in rat aortic smooth muscle strips. Furthermore, although the continued amplitude modulation (AM) type of electrical stimulation was not increased significantly by electrical stimulation, the current of the frequency modulation (FM) type of low frequency electrical stimulation increased the serum $17\beta$-estradiol concentration in normal volunteers. Conclusion: These results, in part, suggest that $17\beta$-estradiol has the capacity to supress stressor-induced muscle tension, and electrical stimulation, particularly current of the FM type, has a modulatory effect on the sex steroid hormones, particularly $17\beta$-estradiol, in healthy volunteers.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2007년도 학술발표회 논문집
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• pp.15-21
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• 2007

Due to a decreasing tendency of river runoff in the Laohahe River basin in North China, quantitative analysis was made with the aid of the conceptual Xinanjiang model under the background of nature climate variability as well as human-induced climate change according to the long-term observational hydrometeorological data. In the past, the human effect on surface water resources was estimated by investigating the impact of human activities on each item in the equation of water balance, so as to calculate water quantity of each item in the original natural status. It seems to be clear conceptually. It is appropriate just for the case of direct impact, such as water transfer from one basin to another, water storage by various scales of hydraulic projects, besides a huge amount of investigation and indeterminate statistics data when applied in practice. It is difficult for us to compute directly water consumption due to the implementation of measures for soil conservation, the improvement of farming techniques in agriculture, the growth of population in towns and villages, and the change of socioeconomic structure. In view of such situation, the Xinanjiang model was used to separate human impact from the climatic impact on water resources. Quantitatively human activity made river runoff decrease by 1.02, 50.67, 58.06 mm in 1960's, 1970's, 1980's, respectively, while by 97.2 mm in 1990's in the sense of annual average in the Laohahe River basin.

• 한국지하수토양환경학회지:지하수토양환경
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• 제15권6호
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• pp.114-121
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• 2010

Oxygen sensitivity and substrate requirement have been known as possible reasons for the intricate growth of Dehalococcoides spp. and limiting factors of for routinely applying bioaugmentation using anaerobic Dehalococcoides-containing microbes for remediating chlorinated organic compounds. To explore the effect of the short-term exposure of the short-term exposure of oxygen on Dehalococcoides capability, dechlorination performance, and hydrogen production fermentation from formate, an anaerobic reductive dechlorination mixed-culture (Evanite culture) including dehalococcoides spp. was in this study. In the results, once the mixed-culture were exposed to oxygen, trichloroethylene (TCE) degradation rate decreased and it was not fully recovered even addition of excess formate for 40 days. In contrast, hydrogen was continuously produced by hydrogen-fermentation process even under oxygen presence. The results indicate that although the oxygen-exposed cells cannot completely dechlorinate TCE to ethylene (ETH), hydrogen fermentation process was not affected by oxygen presence. These results suggest that dechlorinating microbes may more sensitive to oxygen than fermenting microbes, and monitoring dechlorinators activity may be critical to achieve an successful remediation of a TCE contaminated-aquifer through bioaugmentation using Dehalococcoides spp..

• 대한미생물학회지
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• 제35권4호
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• pp.299-308
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• 2000

Recently developed cDNA microarray or DNA chip technology allows expression monitoring of expression of hundreds and thousands of genes simultaneously and provides a format for identifying genes as well as changes in their activity. In order to search for changes in gene expression after X irradiation in HL60 cells, cDNA microarray technique was done. In this study, expression of 588 human genes (including oncogenes, tumor suppressor genes, cell cycle regulator genes, intracellular signal transduction modulator genes, apoptosis related genes, transcription factor genes, growth factors and receptor genes, cytokine genes, etc) were analyzed. For cDNA microarray analysis mRNAs were extracted from control and 8 Gy-irradiated HL60 cells. As a result the changes in expression of several genes were observed. This alteration of gene expression was confirmed by reverse transcription-polymerase chain reaction. The expression of heat shock 60 KD protein, c-jun, erythroid differentiation factor, CPP32, myeloid cell nuclear differentiation antigen, MAP kinase-activated protein kinase, interleukin-8, monocyte chemotactic peptide 1 and RANTES genes was increased, but the expression of p55CDC gene was decreased after X irradiation.