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Effects of Short-Term Oxygen Exposure on Anaerobic Reductive Dechlorination and Formate Fermentation by Evanite Culture  

Hong, Ui-Jeon (Department of Environmental Engineering, Korea University)
Park, Sun-Hwa (Research Institute for Environ Technology and Sustainable Department, Korea University)
Lim, Jong-Hwan (Department of Environmental Engineering, Korea University)
Ahn, Hong-Il (SK Energy CO., LTD.)
Kim, Nam-Hee (SK Energy CO., LTD.)
Lee, Suk-Woo (SK Energy CO., LTD.)
Kim, Young (Department of Environmental Engineering, Korea University)
Publication Information
Journal of Soil and Groundwater Environment / v.15, no.6, 2010 , pp. 114-121 More about this Journal
Abstract
Oxygen sensitivity and substrate requirement have been known as possible reasons for the intricate growth of Dehalococcoides spp. and limiting factors of for routinely applying bioaugmentation using anaerobic Dehalococcoides-containing microbes for remediating chlorinated organic compounds. To explore the effect of the short-term exposure of the short-term exposure of oxygen on Dehalococcoides capability, dechlorination performance, and hydrogen production fermentation from formate, an anaerobic reductive dechlorination mixed-culture (Evanite culture) including dehalococcoides spp. was in this study. In the results, once the mixed-culture were exposed to oxygen, trichloroethylene (TCE) degradation rate decreased and it was not fully recovered even addition of excess formate for 40 days. In contrast, hydrogen was continuously produced by hydrogen-fermentation process even under oxygen presence. The results indicate that although the oxygen-exposed cells cannot completely dechlorinate TCE to ethylene (ETH), hydrogen fermentation process was not affected by oxygen presence. These results suggest that dechlorinating microbes may more sensitive to oxygen than fermenting microbes, and monitoring dechlorinators activity may be critical to achieve an successful remediation of a TCE contaminated-aquifer through bioaugmentation using Dehalococcoides spp..
Keywords
Dehalococcoides spp.; Bioaugmentation; Anaerobic reductive dechlorination; Evanite culture; Trichloroethylene (TCE); Oxygen exposure;
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