• KSII Transactions on Internet and Information Systems (TIIS)
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• 제7권11호
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• pp.2839-2852
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• 2013

Context-awareness is an essential part of ubiquitous computing, and over the past decade video based activity recognition (VAR) has emerged as an important component to identify user's context for automatic service delivery in context-aware applications. The accuracy of VAR significantly depends on the performance of the employed human body segmentation algorithm. Previous human body segmentation algorithms often engage modeling of the human body that normally requires bulky amount of training data and cannot competently handle changes over time. Recently, active contours have emerged as a successful segmentation technique in still images. In this paper, an active contour model with the integration of Chan Vese (CV) energy and Bhattacharya distance functions are adapted for automatic human body segmentation using depth cameras for VAR. The proposed technique not only outperforms existing segmentation methods in normal scenarios but it is also more robust to noise. Moreover, it is unsupervised, i.e., no prior human body model is needed. The performance of the proposed segmentation technique is compared against conventional CV Active Contour (AC) model using a depth-camera and obtained much better performance over it.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1325-1329
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• 2006

Structural modeling of $\beta$-galactosidase from L. lactis ssp. lactis 7962 has shown that the residues Cys-472 and His-509 are located in the wall of the active-site cavity. To examine the functions of Cys-472 and His-509, we generated five site-specific mutants: Cys-472-Ser, Cys-472-Thr, Cys-472-Met, His-509-Asn, and His-509-Phe. $\beta$-Galactosidase substituted at Cys-472 with Met or His-509 with Phe had <3% of the activity of the native enzyme when assayed using ONPG as substrate. The other mutants Cys-472-Ser, Cys-472-Thr, and His-509-Asn had ca. 10-15% of the native enzyme activity. The V$_max$ values of the five mutated enzymes were lower (60-7,000-fold) than that of native enzyme. These results show that the catalytic ability of $\beta$-galactosidase is significantly affected by mutations at Cys-472 or His-509.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1229-1243
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• 2013

In this paper, an air isolate (NITT6L) has been screened based on hemolytic activity, emulsification activity, drop collapsing test, and oil displacement test, as well as lipase activity. It was found that strain NITT6L was able to reduce the surface tension of the medium from 61.5 to 39.83 mN/m and could form stable emulsions with tested vegetable oils. Morphological, biochemical, 16S rRNA sequencing analyses, and fatty acid methyl ester analysis using gas chromatography confirmed that the air isolate under study was Pseudomonas aeruginosa. Characterization of the biosurfactant using agar double diffusion assay revealed that the biosurfactant was anionic in nature, and CTAB-methylene blue assay and Molisch test revealed its glycolipid nature. The FT-IR spectrum confirmed that the crude biosurfactant was a rhamnolipid. Using unoptimized medium containing sucrose as the carbon source, the isolate was found to produce 0.3 mg/ml of rhamnolipid in batch cultivation (shake flask) at $37^{\circ}C$ and pH 7. Optimization of the medium components was carried out using design of experiments and the yield of rhamnolipid has been enhanced to 4.6 mg/ml in 72 h of fermentation.

• 공학교육연구
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• 제10권3호
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• pp.93-108
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• 2007

연구의 목적은 효율적인 기술적 문제해결과 관련 있는 특성을 구명하는 것이다. 연구의 목적을 달성위하여 대전 소재 C대학교 공과대학 재학생을 대상으로 기술적 문제해결 활동을 분석하고 효율적인 문제해결자와 비효율적인 문제해결자의 문제해결 활동을 비교하였다. 그 결과, 다음과 같은 결론을 도출하였다. 기술적 문제의 효율적인 문제해결자와 비효율적인 문제해결자는 문제해결 활동 중에서 해결 방안 모형화하기 활동과 문제점 확인하기 활동의 소요 시간, 결과 평가하기 활동의 빈도와 소요 시간에서 차이를 보인다. 효율적인 문제해결자가 비효율적인 문제해결자보다 해결방안 모형화하기 활동과 문제점 확인하기 활동에 더 많은 시간을 소요하며 결과 평가하기 활동은 낮은 빈도와 적은 시간을 소비한다.

• Molecules and Cells
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• 제19권1호
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• pp.124-130
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• 2005

Protein kinase CKII is composed of two catalytic (${\alpha}$ or ${\alpha}^{\prime}$) subunits and two regulatory (${\beta}$) subunits. The ${\beta}$ subunit mediates tetramer formation through ${\beta}-{\beta}$ homodimerization and ${\alpha}-{\beta}$ heterodimerization. In a previous study R26 and R75, point mutants of $CKII{\beta}$ defective in ${\beta}-{\beta}$ dimerization, were isolated. In the present work we characterized these $CKII{\beta}$ mutants in vitro. Purified R26 and R75 bound to $CKII{\alpha}$ but were defective in binding to $CKII{\beta}$. R75 stimulated the catalytic activity of CKII whereas R26 gave little stimulation, and poly-L-lysine increased the stimulation of catalytic activity by R26 or R75. Circular dichroism and intrinsic fluorescence data pointed to different conformational changes in R26 and R75. Molecular modeling of these mutants provides an explanation of the difference in their ability to interact with $CKII{\beta}$ and to activate $CKII{\alpha}$.

• 한국IT서비스학회지
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• 제21권1호
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• pp.103-125
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• 2022

This study empirically examines the correlation between the data, system, service quality, collective intelligence, innovation activity, and work performance of the KMS for employees of state-run enterprises. This study also confirmed that collective intelligence and innovation activity have a mediating effect on the relationship between KMS system quality and work performance. For this study, employees of state-run enterprises completed a 5-point Likert scale survey, and the data obtained were analyzed with descriptive statistics. The reliability and validity of variables were also verified through a measurement model analysis. Structural equation modeling of IBM SPSS 26 and AMOS 26 were used to prove the hypothesis. Through this study, For the improvement of sustainable work performance of state-run enterprises, which is a difficult issue to solve, not only should the quality of the KMS utilized in the enterprises be improved and managed, but also the design for various incentive schemes tailored to the enterprises' job characteristics, such as rewards, HR bonus, work incentives, job allowance, and encouragement from the head of the enterprise, should be reviewed and continuously developed in reflection of the active collective intelligence of the employees and the effect of innovation activities. Then, the work performance of public institutions will increase.

• Journal of Dairy Science and Biotechnology
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• 제41권2호
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• pp.45-56
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• 2023

This review serves two purposes: first, to promote the use of improved optimization techniques in response surface methodology (RSM); and second, to enhance the optimum conditions for the fermentation of probiotics. According to research in dairy science, Lactiplantibacillus plantarum K79 is a candidate probiotic that has beneficial health effects, such as lowering blood pressure. The optimum conditions for L. plantarumK79 to produce peptides with angiotensin-converting enzyme (ACE) inhibitory activity were proposed, through modeling each of ACE inhibitory activity and pH as a function of the four factors that are skim milk concentration (%), incubation temperature (℃), incubation time (hours), and starter added amount (%). To estimate optimum conditions, the researchers employed a desirability-based multi-response optimization approach, utilizing third-order models with a nonsignificant lack of fit. The estimated optimum fermentation conditions for L. plantarum K79 were as follows: a skim milk concentration of 10.76%, an incubation temperature of 36.9℃, an incubation time of 23.76 hours, and a starter added amount of 0.098%. Under these conditions, the predicted ACE inhibitory activity was 91.047%, and the predicted pH was 4.6. These predicted values achieved the objectives of the multi-response optimization in this study.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2022년도 봄 학술논문 발표대회
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• pp.204-205
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• 2022

Recently, the application of BIM has been continuously expanded according to various government policies, and the application performance is being analyzed in BIM projects such as roads and railways. In line with this trend, the government is making additional efforts to introduce full-scale design BIM. The BIM design in the public sector is directly related to the estimated price. However, looking at the current status of BIM introduction, there is no support system in place for calculating the estimated price. Therefore, this study intends to analyze the priority activity that is easy to develop BIM unit price among the construction standard unit Price of construction work. In this study, in order to derive the BIM-based priority activity, BIM application statements and application guidelines were analyzed. As a result of the analysis, cast-in-place concrete construction (civil engineering) and reinforced concrete construction (architecture) were deduced as suitable construction activities for the preferential application of BIM. Since the results presented in this study are a rough analysis, it is judged that additional detailed analysis is necessary for the future.

• 국제학술발표논문집
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• The 9th International Conference on Construction Engineering and Project Management
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• pp.975-983
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• 2022

This paper presents an innovative way of integrating scheduling and project controls with the environmental impact of a construction project to track, monitor, and manage environmental emissions at the activity level. As a starting point, scheduling and project controls help monitor the status of a project to provide an assessment of the duration and sequence of activities. Additionally, project schedules can also reflect resource allocation and costs associated with various phases of a construction project. Owners, contractors and construction managers closely monitor tasks or activities on the critical path(s) and/or longest path(s) calculated through network based scheduling techniques. However, existing industry practices do not take into account environmental impact associated with each activity during the life cycle of a project. Although the environmental impact of a project may be tracked in various ways, that tracking is not tied to the project schedule and, as such, generally is not updated when schedules are revised. In this research, a Cradle to Gate approach is used to estimate environmental emissions associated with each activity of a sample project schedule. The research group has also investigated the potential determination of scenarios of lowest environmental emissions, just as project managers currently determine scenarios with lowest cost or time. This methodology can be scaled up for future work to develop a library of unit emissions associated with commonly used construction materials and equipment. This will be helpful for project owners, contractors, and construction managers to monitor, manage, and reduce the carbon footprint associated with various projects.

• 정보처리학회논문지D
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• 제10D권3호
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• pp.471-488
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• 2003

최근의 대부분의 정보시스템은 웹 기반 정보시스템이며 이의 개발과 유지보수시에 “웹 위기” 현상이 발생하고 있다 이를 해결하기 위해, 웹 엔지니어링 기술 중 웹 기반 비즈니스 프로세스로의 리엔지니어링 기술이 필요하다. 본 논문에서는 웹 기반 정보시스템으로의 이주와 웹 기반 비즈니스 프로세스 모형화를 위한, BPN(Business Process Net) 모형과 이를 이용한 리엔지니어링 휴리스틱스를 적용 예와 함께 제시한다. BPN모형은 베타분포형 확률 패트리넷이며 수행가능형 Activity diagram이라 할 수 있다. BPN을 모형화 할 때, Use Case 분석을 이용하며 비즈니스 프로세스의 수행시간 및 비용적 불확실성은 베타분포를 이용하고 있다. BPN 모형은 XML 기반의 비즈니스 프로세스의 명세언어를 위한 공통 명세모형으로 이용될 수 있으며, 제시한 리엔지니어링 방법 및 지침은 웹 기반 정보시스템의 분석 및 설계단계에서 적용할 수 있다.