• Proceedings of the KIPE Conference
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• 2012.11a
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• pp.249-250
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• 2012

해상 풍력 발전은 가장 유망한 재생 에너지원의 하나이며, 육상 풍력 발전보다 풍력이 강력하고 일정하여 장시간 고출력 발생이 가능하다. 또한 소음, 공간적 한계, 경관훼손 등 기존 육상 풍력 발전의 단점을 보완하고 초대형 단지조성이 가능한 장점이 있다. 초대형 해상풍력단지에는 일반적으로 MW급의 해상풍력발전기가 사용된다. 본 논문에서는 MW급의 해상 풍력발전기에 ANPC(Active Neutral-Point-Clamped) Multi-Level VSC(Voltage Source Converter)를 적용하여 Back-to-Back으로 구성한 시스템을 제안하고 계통연계형 풍력 발전 시스템을 모의한 시뮬레이션을 통하여 제안된 시스템의 성능을 검증한다.

• Molecules and Cells
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• v.43 no.12
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• pp.1011-1022
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• 2020

Cell type specification is a delicate biological event in which every step is under tight regulation. From a molecular point of view, cell fate commitment begins with chromatin alteration, which kickstarts lineage-determining factors to initiate a series of genes required for cell specification. Several important neuronal differentiation factors have been identified from ectopic over-expression studies. However, there is scarce information on which DNA regions are modified during induced pluripotent stem cell (iPSC) to neuronal progenitor cell (NPC) differentiation, the cis regulatory factors that attach to these accessible regions, or the genes that are initially expressed. In this study, we identified the DNA accessible regions of iPSCs and NPCs via the Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq). We identified which chromatin regions were modified after neuronal differentiation and found that the enhancer regions had more active histone modification changes than the promoters. Through motif enrichment analysis, we found that NEUROD1 controls iPSC differentiation to NPC by binding to the accessible regions of enhancers in cooperation with other factors such as the Hox proteins. Finally, by using Hi-C data, we categorized the genes that directly interacted with the enhancers under the control of NEUROD1 during iPSC to NPC differentiation.

• Journal of Power Electronics
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• v.14 no.1
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• pp.175-182
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• 2014

Tracking harmonic current quickly and precisely is one of the keys to designing active power filters (APF). In the past, the current state feedback decoupling PI control was an effective means for three-phase systems in the current control of constant voltage constant frequency inverters and high frequency PWM reversible rectifiers. This paper analyzes in detail the limitation of the conventional PI conditioner in the APF application field and presents a novel PI control method. Canceling the delay of one sampling period and the misadjustment for tracking the harmonic current is the key problem of this PI control. In this PI control, the predictive output current value is obtained by a state observer. The delay of one sampling period is remedied in this digital control system by the state observer. The predictive harmonic command current value is obtained by a repetitive predictor synchronously. The repetitive predictor can achieve better predictions of the harmonic current. By this means, the misadjustment of the conventional PI control for tracking the harmonic current is cancelled. The experiment results with a three-level NPC APF indicate that the steady-state accuracy and dynamic response of this method are satisfying when the proposed control scheme is implemented.

• The Transactions of the Korean Institute of Power Electronics
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• v.26 no.5
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• pp.315-324
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• 2021

A solid state transformer (SST) that can interface an MVAC of three-phase 13.2 kV and a 1.5 kV DC distribution. SST consists of an AC/DC converter and a DC/DC converter with a high-frequency isolation transformer (HFIT). The AC/DC converter consists of cascaded NPC full-bridge to cope with the MVAC. The DC/DC converter applies a quad active bridge (QAB) topology to reduce the number of the HFIT. Topology analysis and controller design for this specific structure are discussed. In addition, the insulation of HFIT used in DC/DC converters is considered. The discussion is validated using a 300 kVA three-phase SST prototype.

• Journal of Power Electronics
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• v.16 no.5
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• pp.1950-1963
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• 2016

A novel open-circuit fault diagnosis method for 3-phase 4-wire 3-level active power filters based on voltage error correlation is proposed in this paper. This method is based on observing the output pole voltage error of the active power filter through two kinds of algorithms. One algorithm is a voltage error analytical algorithm, which derives four output voltage error analytic expressions through the pulse state, current value and dc bus voltage, respectively, assuming that all of the IGBTs of a certain phase come to an OC fault. The other algorithm is a current circuit equation algorithm, which calculates the real-time output voltage error through basic circuit theory. A correlation is introduced to measure the similarity of the output voltage errors between the two algorithms, and OC faults are located by the maximum of the correlations. A FPGA has been chosen to implement the proposed method due to its fast prototyping. Simulation and experimental results are presented to show the performance of the proposed OC fault diagnosis method.

• Journal of Korea Multimedia Society
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• v.10 no.12
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• pp.1714-1725
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• 2007

This paper describes a top-down behavior planning framework in a simulation game from personality to real life action selection. The combined behavior creating system is formed by five levels of specification, which are personality definition, motivation extraction, emotion generation, decision making and action execution. Along with the data flowing process in our designed framework, NPC selects actions autonomously to adapt to the dynamic environment information resulting from active agents and human players. Furthermore, we illuminate applying Gaussian probabilistic distribution to realize character's behavior changeability like human performance. To elucidate the mechanism of the framework, we situated it in a restaurant simulation game.

• Proceedings of the KIPE Conference
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• 2004.07a
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• pp.475-478
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• 2004

본 논문에서는 부하단에서 발생하는 고조파를 검지하는 방법으로 순시 무효전력 방법(IRPM: Instantaneous Reactive Power Method)을 사용하였다. 이 방식은 DQ변환을 통해 순시 무효 전력을 사용하여 고조파를 검지하는 방식이다. 그러나 순시 무효전력 방식은 3상 인버터를 기반으로 하는 DQ변환 방식을 이용하기 때문에 단상 시스템에는 적용될 수 없다. 본 논문에서는 단상에서도 임의의 가상축을 만들어 DQ변환을 수행할 수 있도록 함으로써 순시무효전력 보상 방식을 단상 능동 전력 필터 시스템에도 적용될 수 있는 방안을 제시하였다. 또한 검지된 고조파를 효과적으로 보상할 수 있는 3레벨 NPC 타입 멀티레벨 인버터를 사용하여 고전력 능동 필터를 구성할 수 있도록 하였다.

• Journal of Power Electronics
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• v.16 no.5
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• pp.1964-1980
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• 2016

This paper presents a new simplified harmonics extraction algorithm based on the synchronous reference frame (SRF) for an indirect current controlled (ICC) three-level neutral point diode clamped (NPC) inverter-based shunt active power filter (SAPF). The shunt APF is widely accepted as one of the most effective current harmonics mitigation tools due to its superior adaptability in dynamic state conditions. In its controller, the SRF algorithm which is derived based on the direct-quadrature (DQ) theory has played a significant role as a harmonics extraction algorithm due to its simple implementation features. However, it suffers from significant delays due to its dependency on a numerical filter and unnecessary computation workloads. Moreover, the algorithm is mostly implemented for the direct current controlled (DCC) based SAPF which operates based on a non-sinusoidal reference current. This degrades the mitigation performances since the DCC based operation does not possess exact information on the actual source current which suffers from switching ripples problems. Therefore, three major improvements are introduced which include the development of a mathematical based fundamental component identifier to replace the numerical filter, the removal of redundant features, and the generation of a sinusoidal reference current. The proposed algorithm is developed and evaluated in MATLAB / Simulink. A laboratory prototype utilizing a TMS320F28335 digital signal processor (DSP) is also implemented to validate effectiveness of the proposed algorithm. Both simulation and experimental results are presented. They show significant improvements in terms of total harmonic distortion (THD) and dynamic response when compared to a conventional SRF algorithm.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.265-270
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• 2010

Two major endoglucanase genes (cel7B and cel5A) were cloned from Penicillium decumbens 114-2 using the method of modified thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR). The result of Southern blotting suggested that P. decumbens has a single copy of the cel5A gene and a single copy of the cel7B gene in its chromosomal DNA. The expression levels of cel5A and cel7B were determined by means of real-time quantitative PCR, suggesting that the two genes were coordinately expressed, and repressed by glucose and induced by cellulose. Both endoglucanase genes were expressed in Saccharomyces cerevisiae and the recombinant proteins were purified. The recombinant Cel7B and Cel5A were both optimally active at $60^{\circ}C$ and pH 4.0. The recombinant Cel7B showed more than 8-fold, 30-fold, and 5-fold higher enzyme activities toward carboxymethyl cellulose, barley $\beta$-glucan, and PASC, respectively, in comparison with that of Cel5A. However, their activities toward pNPC and Avicel showed minor differences. The results suggested that Cel7B is a strict endoglucanase, whereas Cel5A showed processivity because of its relative higher ability to hydrolyze the crystal cellulose.

• Proceedings of the Microbiological Society of Korea Conference
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• 2001.11a
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• pp.16-25
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• 2001

A genomic library of biphenyl-degrading strain Comamonas sp. SMN4 was constructed by using the cosmid vector pWE15 and introduced into Escherichia coli. Of 1,000 recombinant clones tested, two clones that expressed 2,3-dihydroxybiphenyl 1,2-dioxygenase activity were found (named pNB 1 and pNB2). From pNB1 clone, subclone pNA210, demonstrated 2,3-dihydroxybiphenyl 1,2-dioxygenase activity, is isolated. 2,3-Dihydroxybiphenyl 1,2-dioxygenase (23DBDO, BphC) is an extradiol-type dioxygenase that involved in third step of biphenyl degradation pathway. The nucleotide sequence of the Comamonas sp. SMN4 gene bphC, which encodes 23DBDO, was cloned into a plasmid pQE30. The His-tagged 23DBDO produced by a recombinant Escherichia coli, SG 13009 (pREP4)(pNPC), and purified with a Ni-nitrilotriacetic acid resin affinity column using the His-bind Qiagen system. The His-tagged 23DBDO construction was active. SDS-PAGE analysis of the purified active 23DBDO gave a single band of 32 kDa; this is in agreement with the size of the bphC coding region. The 23DBDO exhibited maximum activity at pH 9.0. The CD data for the pHs, showed that this enzyme had a typical a-helical folding structures at neutral pHs ranged from pH 4.5 to pH 9.0. This structure maintained up to pH 10.5. However, this high stable folding strucure was converted to unfolded structure in acidic region (pH 2.5) or in high pH (pH 12.0). The result of CD spectra observed with pH effects on 23DBDO activity, suggested that charge transition by pH change have affected change of conformational structure for 23DBDO catalytic reaction. The $K_m$ for 2,3-dihydroxybiphenyl, 3-metylcatechol, 4-methylcatechol and catechol was 11.7 $\mu$M, 24 $\mu$M, 50 mM and 625 $\mu$M.