• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 춘계학술대회 논문집
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• pp.2271-2302
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• 2008

The purpose of this research is to develop a tourism product that connects Northeast Asia and Korea by maximizing the geographical advantages in North Korea. Although Northeast Asia has enjoyed the tremendous amounts of potentials such as the natural, historical and cultural resources, some political and social limitations have prohibited its wider development of Tourism.

• The Korean Journal of Physiology and Pharmacology
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• 제20권1호
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• pp.91-99
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• 2016

(E)-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone (MPP) is an aldol condensation product resulting from pyrrole-2-carbaldehyde and m- and p- substituted acetophenones. However, its biological activity has not yet been evaluated. Since it has been reported that some propenone-type compounds display anti-inflammatory activity, we investigated whether MPP could negatively modulate inflammatory responses. To do this, we employed lipopolysaccharide (LPS)-stimulated macrophage-like RAW264.7 cells and examined the inhibitory levels of nitric oxide (NO) production and transcriptional activation, as well as the target proteins involved in the inflammatory signaling cascade. Interestingly, MPP was found to reduce the production of NO in LPS-treated RAW264.7 cells, without causing cytotoxicity. Moreover, this compound suppressed the mRNA levels of inflammatory genes, such as inducible NO synthase (iNOS) and tumor necrosis factor (TNF)-${\alpha}$. Using luciferase reporter gene assays performed in HEK293 cells and immunoblotting analysis with nuclear protein fractions, we determined that MPP reduced the transcriptional activation of nuclear factor (NF)-${\kappa}B$. Furthermore, the activation of a series of upstream signals for NF-${\kappa}B$ activation, composed of Src, Syk, Akt, and $I{\kappa}B{\alpha}$, were also blocked by this compound. It was confirmed that MPP was able to suppress autophosphorylation of overexpressed Src and Syk in HEK293 cells. Therefore, these results suggest that MPP can function as an anti-inflammatory drug with NF-${\kappa}B$ inhibitory properties via the suppression of Src and Syk.

• Archives of Pharmacal Research
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• 제29권10호
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• pp.840-844
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• 2006

For the exploration of pharmacophoric moiety of malloapelta B (1) possessing the inhibitory activity of $NF-{\kappa}B$ activation, structural variation of ${\alpha},{\beta}-unsaturated$ carbonyl motif was attempted. 1 was reduced by catalytic hydrogenation, sodium borohydride, and lithium aluminumhydride. Catalytic hydrogenation with 30 psi or 15 psi of $H_2$ gas of 1 generated 8-butyl-5,7-dimethoxy-2,2-dimethylchroman (2) and 1-(5,7-dimethoxy-2,2-dimethylchroman-8-yl)butan-1-one (3), respectively. Reduction with sodium borohydride occurred at the double bond of ${\alpha},{\beta}-unsaturated$ ketone of 1 to give 1-(5,7-dimethoxy-2,2-dimethyl-2H-chromen-8-yl)butan-1-one (4). Reduction of 1 with lithium aluminumhydride and then quenched with methanol and water produced unexpected products, 1-(5,7-dimethoxy-2,2-dimethyl-2H-chromen-8-yl)-3-methoxy-1-butene (5) and 1-(5,7-dimethoxy-2,2-dimethyl-2H-chromen-8-yl)-3-hydroxy-1-butene (6). These are formed from the isomerization of initial product 9 through the continuous conjugate carbocation intermediate 11. Addition of ethylmagnesium bromide and dimethyl malonate anion to 1 gave the conjugate adducts 7 and 8. Ethylmagesium bromide and sodium borohydride reduction unusually gave the conjugate addition due to steric congestion around carbonyl group of 1. Compound 2 exhibits the reduced inhibitory activity against $NF-{\kappa}B$ activation and the others do not show the activity. Therefore ${\alpha},{\beta}-unsaturated$ carbonyl group of 1 should be important for its inhibitory activity.

• Toxicological Research
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• 제33권4호
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• pp.283-290
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• 2017

The host immune system is the first line of host defense, consisting mainly of innate and adaptive immunity. Immunity must be maintained, orchestrated, and harmonized, since overactivation of immune responses can lead to inflammation and autoimmune diseases, while immune deficiency can lead to infectious diseases. We investigated the regulation of innate and adaptive immune cell activation by Artemisia capillaris and its components (ursolic acid, hyperoside, scopoletin, and scopolin). Macrophage phagocytic activity was determined using fluorescently labeled Escherichia coli, as an indicator of innate immune activation. Concanavalin A (ConA)- and lipopolysaccharide (LPS)-induced splenocyte proliferation was analyzed as surrogate markers for cellular and humoral adaptive immunity, respectively. Neither A. capillaris water extract (WAC) nor ethanol extract (EAC) greatly inhibited macrophage phagocytic activity. In contrast, WAC suppressed ConA- and LPS-induced proliferation of primary mouse splenocytes in a dose-dependent manner. Similarly, EAC inhibited ConA- and LPS-induced splenocyte proliferation. Oral administration of WAC in mice decreased ConA- and LPS-induced splenocyte proliferation, while that of EAC suppressed LPS-induced splenocyte proliferation. Repeated administration of WAC in mice inhibited ConA- and LPS-induced splenocyte proliferation. Ursolic acid, scopoletin, and scopolin reduced ConA- and LPS-induced primary mouse splenocyte proliferation, while hyperoside did not show such activity. These results indicate that A. capillaris and its components, ursolic acid, scopoletin, and scopolin, suppress ConA- and LPS-induced adaptive immune cell activation. The results suggest that A. capillaris is useful as a regulator of adaptive immunity for diseases involving excessive immune response activation.

• Nutrition Research and Practice
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• 제11권4호
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• pp.275-280
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• 2017

BACKGROUND/OBJECTIVES: Re-epithelialization has an important role in skin wound healing. Astaxanthin (ASX), a carotenoid found in crustaceans including shrimp, crab, and salmon, has been widely used for skin protection. Therefore, we investigated the effects of ASX on proliferation and migration of human skin keratinocyte cells and explored the mechanism associated with that migration. MATERIAL/METHOD: HaCaT keratinocyte cells were exposed to $0.25-1{\mu}g/mL$ of ASX. Proliferation of keratinocytes was analyzed by using MTT assays and flow cytometry. Keratinocyte migration was determined by using a scratch wound-healing assay. A mechanism for regulation of migration was explored via immunocytochemistry and western blot analysis. RESULTS: Our results suggest that ASX produces no significant toxicity in human keratinocyte cells. Cell-cycle analysis on ASX-treated keratinocytes demonstrated a significant increase in keratinocyte cell proliferation at the S phase. In addition, ASX increased keratinocyte motility across the wound space in a time-dependent manner. The mechanism by which ASX increased keratinocyte migration was associated with induction of filopodia and formation of lamellipodia, as well as with increased Cdc42 and Rac1 activation and decreased RhoA activation. CONCLUSIONS: ASX stimulates the migration of keratinocytes through Cdc42, Rac1 activation and RhoA inhibition. ASX has a positive role in the re-epithelialization of wounds. Our results may encourage further in vivo and clinical study into the development of ASX as a potential agent for wound repair.

• 한국콘텐츠학회논문지
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• 제18권2호
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• pp.73-80
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• 2018

본 연구는 농촌관광객을 대상으로 농촌문화관광요소가 농산물브랜드 이미지에 미치는 영향을 알아보고자 하였으며, 연구결과는 다음과 같다. 첫째, 하드웨어 요소는 부분적으로 농산물 브랜드 이미지에 정(+)의 영향을 미치는 것으로 나타났다. 둘째, 소프트웨어 요소는 부분적으로 농산물 브랜드 이미지에 정(+)의 영향을 미치는 것으로 나타났다. 셋째, 인적자원 요소는 부분적으로 농산물 브랜드 이미지에 정(+)의 영향을 미치는 것으로 나타났다. 이러한 결과를 통해 농산물 브랜드 이미지에 영향을 미치는 각각의 농촌문화관광요소에 대해 다양한 전략을 개발하여 농촌문화관광의 활성화 전략개발 등이 이루어져야 할 것이다.

• 한국환경과학회지
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• 제17권3호
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• pp.351-357
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• 2008

In order to develope an efficient way for the synthesis of highly pure 1,4-sorbitan solution from sorbitol, some experimental studies were performed. The reaction showed first order reaction with activation energy of 118.3 KJ/mol. Color of the product solutions changed to brown with reaction temperature and reaction time. The equilibrium contents of 1,4-sorbitan increased with decrease in reaction pressure, but the content of major impurity, sorbide, showed maximum about 550 torr vacuum with $H_3PO_4$ catalyst. The reasonable catalyst configuration was 0.26 wt% PTSA and 1 wt% $H_3PO_2$ and optimum reaction temperature and pressure range was $110\sim120^{\circ}C$ and $700\sim720$ torr vacuum, respectively. At optimum reaction conditions, we could obtain white product solutions of highly pure 1,4-sorbitan with sorbide less than 10 wt%. This white product solution is advantageous for preparation of high quality span, anti-fogging agent.

• Natural Product Sciences
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• 제27권2호
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• pp.115-121
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• 2021

Lentinus edodes contains functional metabolites such as polysaccharopeptides, lectins, and secondary metabolites. Fermented soybean paste is representative fermented materials in Korea, and is gradually increasing due to various biological activities. In the present study, ethanol extracts of fermented products with/without L. edodes were designated as SPL and SP, and prepared to develop safer and therapeutic functional foods with antioxidant and anti-inflammatory activities for treatment of inflammatory disorders. SP and SPL extracts exhibited antioxidant effects via inhibiting radical activities. Inflammatory mediators, nitric oxide (NO), prostaglandin E₂ (PGE₂), interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) production and nuclear factor-kappa B (NF-κB) activation were down-regulated by two extracts. SPL extract more strongly enhanced the antioxidant and anti-inflammatory activities than SP extract. Its' activities shown more longer fermentation period and more strong inhibitory effects. Taken together, our results suggested that fermented product with medicinal plant has synergic effect and SPL can be a potential candidate for treatment of inflammatory bowel diseases.

• 한국정보처리학회논문지
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• 제7권5S호
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• pp.1726-1735
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• 2000

전자상거래가 활성화되면서 인터넷을 이용한 상품의 구매가 점점 증가하고 있다. 현재 전자상거래에 이용되는 대부분의 상품은 하드웨어가 주를 이루고 있다. 전자파일 형태의 상품 즉, 디지털 상품의 온라인 판매는 물류비용이 없고, 상품의 검색이용이하며, 한시적으로 사용해 본 후 구입할 수 있는등의 많은 장점을 가지고 있지만 불법 복제 문제 때문에 혀재 전자상거래에서는기피 상품으로 취급받고 있다. 디지털 소프트웨어 상품의 불법 복제 문제는 상용권 관리 기술의 부재가 가장 큰 이유중이 하나니 . 현재 이루어지고 있는 전자상거래는 사용권 부여 대상의 식별 방법이 없고, 대상의 이동 방지가 불가능하고, 해킹 방지가 미비하며, 기타 전자상거래 구조가 미완성되어 사용권 관리상에 많은 문제젬을 드러내고 있다. 본 논문에서는 전자상거래 상에서 소프트웨어 상품의 유통을 활성화 할 수 있는 새로운 사용권 관리 기술인 동적 사용권 관리(DLCC: Dynamic License Control)기술을 이용하여 디지털 상품의 유통을 활성화 할 뿐 아니라 불법 복제를 막을 수 있는 디지털 소프트웨어 상품의 상품 작성 시스템의 설계 및 구현을 제안한다.을 제안한다.

• 한국사회복지학
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• 제56권1호
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• pp.155-179
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• 2004

본 연구는 현행 노동부와 한국장애인고용촉진공단이 주도하는 직업훈련사업의 효과성을 분석하고자 실시되었으며 장애인직업훈련시설수료생을 대상으로 설문조사를 실시하였다. 장애인 직업훈련의 효과성은 '생산성 효과'와 '취업효과'로 개념화하였고 '생산성 효과'는 자격증 취득, 자아효능감, 교육성취도 변수로 측정되었으며 분석결과 훈련시설유형, 훈련직종, 훈련기간이 주요한 요인으로 나타났다. '취업효과' 즉 훈련수료 후 취업가능성에 영향을 미치는 요인은 훈련시설로 나타나 훈련생산성보다 훈련시설의 취업노하우와 훈련생관리가 취업에 더 중요한 요인임을 예측할 수 있었다. 따라서 장애인직업 훈련사업의 효과성을 높이기 위해 프로그램과 교육자원을 공유할 수 있도록 공공, 민간, 보건복지부산하 훈련시설의 기능을 재정립하여 훈련시설 간 직업훈련 효과의 격차를 줄이고, 지역별 훈련사업을 특성화시키며, 장애인고용촉진기금의 효율적 운용을 위해 실적에 따른 지원차등화가 필요한 것으로 나타났다.