• 대한본초학회지
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• 제32권5호
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• pp.47-55
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• 2017

Objectives : Obesity is caused by the excess accumulation of fat in the body due to energy imbalance, and it causes various diseases. The aim of this study was to investigate an anti-obesity efficacy and an antioxidant activity of water from herbal mixture extract (SM17). Methods : The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. To evaluated anti-obesity effect of SM17, we used a high fat diet fed mouse model. The SM17 (150 mg/kg body weight/day, p.o.) was treated every day for 6 weeks to C57BL/6 mice. Body weight and food intake were measured every day. The changes of reactive oxygen species (ROS), alanine aminotransferanse (ALT), aspartate aminotransferase (AST), triglycerids (TG) and total cholesterol (TC) in serum were analyzed after experiment. Also, expression of lipid metabolism related proteins were investigated by western blot analysis. Results : It was effective in antioxidant measurements, SM17 administration inhibited the biomarkers of lipid metaboism in serum and tissues. The administration of SM17 showed a significant reduction of body and tissue weight. Morever, it decreased ROS, ALT, AST, TG and TC in serum, compared with those of the obese mice. Adipogenesis-related protein expressions increased in obese mice compared to normal mice. However, SM17 group exhibited the down-expression of these proteins. Conclusion : A SM17 aqueous extract has a great effect on the stimulation (AMPK) activation, and may have a benefit to reduce a fatty acid metabolism through inhibition of lipid accumulation.

• 대한본초학회지
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• 제35권1호
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• pp.57-68
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• 2020

Objective : The aim of present study was to clarify the effect of Areca Semen and Toosendan Fructus Mixture (AT-mix) on chronic reflux esophagitis (CRE) in rats. Methods : The antioxidant activity of AT-mix was measured through DPPH and ABTS radical scavenging activities in vitro. CRE was induced in SD rats (5 weeks, male) by ligating the border forestomach and granular portion with 2-0 silk and the duodenum near the pyloric portion was covered with 2-mm wide piece of 18-Fr Nélaton catheter. And then rats were treated AT-mix 200 mg/kg one daily for 14 days. The anti-oxidant and inflammatory protein levels were evaluated using western blotting. Results : Gross lesion of esophageal mucosa after AT-mix treatment showed a superior enhancement compared with that of CRE control rats. AT-mix treatment strongly reduced both DPPH and ABTS radical scavenging activities (DPPH, IC₅₀ 8.15±0.14 ㎍/mL; ABTS, IC₅₀ 24.69±0.03 ㎍/mL, repspectively). Levels of the NADPH oxidase subunit including NOX4 and p22^phox increased in CRE control rats. Otherwise, AT-mix treatment significantly reduced. The activation of Nuclear factor-erythroid 2-related factor 2 (Nrf2) led to significantly the up-regulation of HO-1. The inhibition of IκBα phosphorylation led to NF-κB inactivation. Subsequently, NF-κB inactivation significantly induced the decrease of COX-2, iNOS, TNF-α, and IL-6 protein expressions. Conclusion : Taken together, these results suggest that AT-mix treatment can attenuate the esophageal mucosal ulcer though inhibiting NF-κB pathway and enhancing Nrf2/HO-1 pathway. Thus, the additional mechanism study about AT-mix would need for the development as a safe herbal therapy for CRE.

• Nutrition Research and Practice
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• 제16권5호
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• pp.589-603
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• 2022

BACKGROUND/OBJECTIVES: Previous studies have reported that protein supplementation contributes to the attenuation of inflammation. Serious trauma such as burn injury usually results in the excessive release of inflammatory factors and organs dysfunction. However, a few reports continued to focus on the function of protein ingestion in regulating burn-induced inflammation and organ dysfunction. MATERIALS/METHODS: This study established the rat model of 30% total body surface area burn injury, and evaluated the function of blended protein (mixture of whey and soybean proteins). Blood routine examination, inflammatory factors, blood biochemistry, and immunohistochemical assays were employed to analyze the samples from different treatment groups. RESULTS: Our results indicated a decrease in the numbers of white blood cells, monocytes, and neutrophils in the burn injury group administered with the blended protein nutritional support (Burn+BP), as compared to the burn injury group administered normal saline supplementation (Burn+S). Expressions of the pro-inflammatory factors (tumor necrosis factor-α and interleukin-6 [IL-6]) and chemokines (macrophage chemoattractant protein-1, regulated upon activation normal T cell expressed and secreted factor, and C-C motif chemokine 11) were dramatically decreased, whereas anti-inflammatory factors (IL-4, IL-10, and IL-13) were significantly increased in the Burn+BP group. Kidney function related markers blood urea nitrogen and serum creatinine, and the liver function related markers alanine transaminase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase were remarkably reduced, whereas albumin levels were elevated in the Burn+BP group as compared to levels obtained in the Burn+S group. Furthermore, inflammatory cells infiltration of the kidney and liver was also attenuated after burn injury administered with blended protein supplementation. CONCLUSIONS: In summary, nutritional support with blended proteins dramatically attenuates the burn-induced inflammatory reaction and protects organ functions. We believe this is a new insight into a potential therapeutic strategy for nutritional support of burn patients.

• 한국심리학회지:법
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• 제11권3호
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• pp.329-347
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• 2020

사람들은 불확실한 상황에서 판단을 내릴 때 외부에서 주어진 정보의 영향을 받아 그 정보의 근사치로 판단을 내리는 경향이 있으며 이를 정박효과(anchoring effect)라고 한다. 본 연구에서는 배심원들이 사법적 의사결정을 내릴 때 나타나는 정박효과를 연구 문제로 설정하였다. 사법적 의사결정 상황에서는 검사구형량이 정박점으로 작용하여 최종 판결되는 양형이 검사구형량과 유사해지는 현상이 나타난다. 본 연구는 배심원 자격이 있는 만 20세 이상의 대학생들을 대상으로 진행되었다. 연구 1에서는 범죄사건의 경중과 정박크기에 따른 정박효과의 차이를 검증하였다. 그 결과 중범죄사건에서 경범죄사건에 비해 정박효과가 더 강하게 나타났다. 또한 경범죄사건의 저정박 조건에서 고정박 조건에 비해 정박효과가 더 강하게 나타났다. 연구 2에서는 정서상태에 따라 정박효과가 달라짐을 검증하였다. 그 결과 분노정서를 느낄 때 슬픔정서를 느낄 때보다 정박효과가 강하게 나타났다. 마지막으로 정박효과를 약화시키는 방안에 대한 연구 3에서는, 정박효과의 접근가능성 모델이 활성화되는 것을 방지할 때 정박효과가 유의미하게 감소하는 것으로 나타났다. 본 연구의 결과들은 배심원의 사법적 의사결정과정에서 나타나는 정박효과와 관련된 문제들을 파악하고 해결하는 데 기여할 수 있을 것이다.

• 대한한의학회지
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• 제44권2호
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• pp.119-131
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• 2023

Objectives: Betula Platyphylla(BP) has been used as a analgesic, anti-microbial, anti-oxidant drug in Eastern Asia. However, it is still unknown whether BP ethanol extract could exhibit the inhibitory activities against ultraviolet B(UVB)-induced skin injury on human keratinocytes, HaCaT cells. This study was aimed to investigate the protective activity of BP ethanol extract on UVB-irradiated skin injury in HaCaT cells. Methods: The skin injury model of HaCaT cells was established under UVB stimulation. HaCaT keratinocyte cells were pre-treated with BP ethanol extract for 1 h, and then stimulated with UVB. Then, the cells were harvested to measure the cell viability, production of reactive oxygen species(ROS), pro-inflammatory cytokines such as interleukin(IL) 1-beta, IL-6, and tumor necrosis factor(TNF)-𝛼, hyaluronidase, type 1 collagen, matrix metalloproteinase(MMP)s. In addition, we examined the mitogen activated protein kinases(MAPKs) and inhibitory kappa B alpha(I𝜅;-B𝛼) as inhibitory mechanisms of BP ethanol extract. Results: The treatment of BP ethanol extract inhibited the UVBinduced cell death and ROS production in HaCaT cells. BP ethanol extract treatment inhibited the UVB-induced increase of IL-1beta, IL-6, and TNF-𝛼. BP ethanol extract treatment inhibited the increase of hyaluronidase, MMP and decrease of collagen. BP ethanol extract treatment inhibited the activation of MAPKs and the degradation of I𝜅-B𝛼. Conclusions: Our result suggest that treatment of BP ethanol extract could inhibit the UVB-induced skin injury via deactivation of MAPKs and nuclear factor kappa B(NF-𝜅B) in HaCaT cells. This study could suggest that BP ethanol extract could be a beneficial agent to prevent skin damage or inflammation.

• Journal of Ginseng Research
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• 제47권4호
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• pp.561-571
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• 2023

Background: Escalating evidence shows that ginseng possesses an antiaging potential with cognitive enhancing activity. As mountain cultivated ginseng (MCG) is cultivated without agricultural chemicals, MCG has emerged as a popular herb medicine. However, little is known about the MCG-mediated pharmacological mechanism on brain aging. Methods: As we demonstrated that glutathione peroxidase (GPx) is important for enhancing memory function in the animal model of aging, we investigated the role of MCG as a GPx inducer using GPx-1 (a major type of GPx) knockout (KO) mice. We assessed whether MCG modulates redox and cholinergic parameters, and memory function in aged GPx-1 knockout KOmice. Results: Redox burden of aged GPx-1 KO mice was more evident than that of aged wild-type (WT) mice. Alteration of Nrf2 DNA binding activity appeared to be more evident than that of NFκB DNA binding activity in aged GPx-1 KO mice. Alteration in choline acetyltransferase (ChAT) activity was more evident than that in acetylcholine esterase activity. MCG significantly attenuated reductions in Nrf2 system and ChAT level. MCG significantly enhanced the co-localization of Nrf2-immunoreactivity and ChAT-immunoreactivity in the same cell population. Nrf2 inhibitor brusatol significantly counteracted MCG-mediated up-regulation in ChAT level and ChAT inhibition (by k252a) significantly reduced ERK phosphorylation by MCG, suggesting that MCG might require signal cascade of Nrf2/ChAT/ERK to enhance cognition. Conclusion: GPx-1 depletion might be a prerequisite for cognitive impairment in aged animals. MCG-mediated cognition enhancement might be associated with the activations of Nrf2, ChAT, and ERK signaling cascade.

• Journal of Veterinary Science
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• 제24권4호
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• pp.52.1-52.13
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• 2023

Background: Mesenchymal stem cells (MSCs) have been investigated as therapeutic agents for inflammatory bowel disease (IBD). Stimulation of MSCs with pro-inflammatory cytokines is an approach to enhance their immunomodulatory effects. However, further investigation is required to support their application in immune-mediated disorders and companion animals. Objectives: This study aimed to assess the therapeutic effect of tumor necrosis factor (TNF)-α-stimulated feline adipose tissue-derived MSCs (fAT-MSCs) in a dextran sulfate sodium (DSS)-induced colitis mouse model. Methods: Colitis mice was made by drinking water with 3% DSS and fAT-MSCs were injected intraperitoneally. Colons were collected on day 10. The severity of the disease was evaluated and compared. Raw 264.7 cells were cultured with the conditioned medium to determine the mechanism, using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Results: TNF-α-stimulated fAT-MSCs more improved severity of DSS-induced colitis in disease activity, colon length, histologic score, and inflammatory cytokine. In sectionized colon tissues, the group comprising TNF-α-stimulated fAT-MSCs had higher proportion of CD11b⁺CD206⁺ macrophages than in the other groups. In vitro, TNF-α-stimulation increased cyclooxygenase-2 (COX-2) expression and prostaglandin E₂ (PGE₂) secretion from fAT-MSCs. The conditioned medium from TNF-α-stimulated fAT-MSCs enhanced the expression of interleukin-10 and arginase-1 in LPS-activated Raw 264.7 cells. Conclusions: These results represent that TNF-α-stimulated fat-mscs ameliorate the inflamed colon more effectively. Furthermore, we demonstrated that the effectiveness was interlinked with the COX-2/PGE₂ pathway.

• Asia Marketing Journal
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• 제7권1호
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• pp.21-42
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• 2005

고객관계관리(customer relationship management: 이하 CRM)는 고객에 대한 정보를 수집하고 수집된 정보를 효과적으로 활용하여 신규고객획득, 우수고객 유지, 고객가치 증진, 잠재고객 활성화, 평생 고객화의 순환을 통하여 고객을 적극적으로 관리하고 유지하며 고객의 가치를 극대화시키기 위한 기업 마케팅 전략의 일환이다. 특히 경쟁 환경이 급변하고 치열해 짐에 따라 기업의 수익 극대화를 위한 고객가치 증대 및 고객과의 관계 형성을 위한 CRM활동 중 고객의 이탈방지를 통한 유지관리의 중요성이 점차 커지고 있으며, 이러한 움직임은 고객 세분화를 통한 이탈고객 관리분석으로 주로 금융시장에서 다루어져왔다. 한편, 금융시장뿐만 아니라 모든 사업 분야에서 고객 유지 및 이탈방지를 위한 분석의 필요성은 높아지고 있다. 그 이유는 자사가 보유하고 있는 고객의 특성을 파악함으로써 기존의 고객을 효과적으로 유지·관리하여 고객이탈을 막는 것이 고객관리에서 점차 그 중요성을 더하기 때문이다. 그러나 아직까지 필요성만 대두될 뿐 어떠한 속성을 보유하고 있는 고객이 쉽게 이탈하는지를 판별할 수 있는 이탈고객에 대한 체계적인 연구가 진행되지 않았다는데 한계점이 있다. 이에 본 연구에서는 TV 홈쇼핑사의 실제 고객자료를 통하여 고객의 유지 및 이탈방지를 위한 CRM전개방안, 이탈고객과 유지고객간의 인구통계적 속성 및 거래 행동의 특성 차이를 분석, 이탈에 미치는 영향력이 높은 변수를 밝혀내고 이탈고객예측 모형을 통하여 개별고객의 이탈확률을 예측하고자 했다. 더 나아가 실증 분석 결과를 바탕으로 이탈예측고객을 대상으로 고객 이탈을 방지하고 거래유지 및 활성화를 위한 CRM전개 방안을 도출, 이를 바탕으로 TV 홈쇼핑사가 수립해야할 마케팅 전략을 제시한다.

• 실천공학교육논문지
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• 제15권2호
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• pp.381-388
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• 2023

스프레드시트를 활용한 인공신경망 교육을 통해, 비전공자 학부생들은 인공신경망의 동작 원리을 이해하며 자신만의 인공신경망 SW를 개발할 수 있다. 여기서, 인공신경망의 동작 원리 교육은 훈련데이터의 생성과 정답 라벨의 할당부터 시작한다. 이후, 인공 뉴런의 발화 및 활성화 함수, 입력층과 은닉층 그리고 출력층의 매개변수들로부터 계산되는 출력값을 학습한다. 마지막으로, 최초 정의된 각 훈련데이터의 정답 라벨과 인공신경망이 계산한 출력값 간 오차를 계산하는 과정을 학습하고 오차제곱의 총합을 최소화하는 입력층과 은닉층 그리고 출력층의 매개변수들이 계산되는 과정을 학습한다. 스프레드시트를 활용한 인공신경망 동작 원리 교육을 비전공자 학부생 대상으로 실시하였다. 그리고 이미지 훈련데이터와 기초 인공신경망 개발 결과를 수집하였다. 본 논문에서는 12화소 크기의 소용량 이미지로 두 가지 훈련데이터와 해당 인공신경망 SW를 수집한 결과를 분석하고, 수집한 훈련데이터를 Orange 머신러닝 모델 학습 및 분석 도구에 활용하는 방법과 실행 결과를 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1213-1227
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• 2023

Fetal growth restriction (FGR) is a prevalent obstetric condition. This study aimed to investigate the role of Toll-like receptor 9 (TLR9) in regulating the inflammatory response and gut microbiota structure in FGR. An FGR animal model was established in rats, and ODN1668 and hydroxychloroquine (HCQ) were administered. Changes in gut microbiota structure were assessed using 16S rRNA sequencing, and fecal microbiota transplantation (FMT) was conducted. HTR-8/Svneo cells were treated with ODN1668 and HCQ to evaluate cell growth. Histopathological analysis was performed, and relative factor levels were measured. The results showed that FGR rats exhibited elevated levels of TLR9 and myeloid differentiating primary response gene 88 (MyD88). In vitro experiments demonstrated that TLR9 inhibited trophoblast cell proliferation and invasion. TLR9 upregulated lipopolysaccharide (LPS), LPS-binding protein (LBP), interleukin (IL)-1β and tumor necrosis factor (TNF)-α while downregulating IL-10. TLR9 activated the TARF3-TBK1-IRF3 signaling pathway. In vivo experiments showed HCQ reduced inflammation in FGR rats, and the relative cytokine expression followed a similar trend to that observed in vitro. TLR9 stimulated neutrophil activation. HCQ in FGR rats resulted in changes in the abundance of Eubacterium_coprostanoligenes_group at the family level and the abundance of Eubacterium_coprostanoligenes_group and Bacteroides at the genus level. TLR9 and associated inflammatory factors were correlated with Bacteroides, Prevotella, Streptococcus, and Prevotellaceae_Ga6A1_group. FMT from FGR rats interfered with the therapeutic effects of HCQ. In conclusion, our findings suggest that TLR9 regulates the inflammatory response and gut microbiota structure in FGR, providing new insights into the pathogenesis of FGR and suggesting potential therapeutic interventions.