• Title/Summary/Keyword: Activated Sludge

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Confirmation of Trichloroethylene-Degrading Enzyme from a Phenol-Degrading Bacterium, Pseudomonas sp. EL-04J (페놀분해세균인 Pseudomonas sp. EL-04J로부터 Trichloroethylene 분해효소의 확인)

  • Park, Geun-Tae;Kim, Ho-Sung;Son, Hong-Ju;Lee, Gun;Park, Sung-Hoon;Lee, Sang-Jun
    • Journal of Life Science
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    • v.12 no.5
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    • pp.561-565
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    • 2002
  • Pseudomonas sp. EL-041 was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). In this study, we report the identification of trichloroethylene- degrading enzyme in Pseudomonas sp. EL-041 by the investigation of enzyme activity and DNA sequencing of specific phenol oxygenase gene. As the results of experiment, trichloroethylene-degrading enzyme in Pseudomonas sp. EL-041 was monooxygenase and suspected to phenol hydroxylase.

A NEW TREATMENT SYSTEM FOR ANIMAL WASTE WATER USING MICROORGANISM, SOIL AND VEGETATION

  • Oshida, T.;Fukuyasu, T.;Kohzaki, K.;Izumikawa, Y.;Kawanabe, S.;Konishi, S.;Oikawa, N.;Matsumoto, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.2
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    • pp.205-209
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    • 1993
  • A new treatment system for animal waste water has been developed as an alternative to the activated sludge process. It consists of two treatments; one is operated with 7 tanks, and the other is soil and plant cultivation bed. Aerobic microorganisms are added to the influent water in the tanks where the water is aerated so that the microbes utilize the pollutants, while sedimentation removes the indigestible solids. In the secondary treatment the water, which has already received a primary treatment, is filtered through soil where it also receives treatment by soil organisms. In addition there is transpiration of water and absorption of minerals by plants. In the primary treatment BOD, SS, coliforms (E. coli), TP and total bacteria were removed 79-99%, but COD and TN were removed only 58% and 36%, respectively. In the secondary treatment removal of nutrients proceeded further, and 93-99% of pollutants were removed. The treated waters met the quality standard of discharge water in Japan except for TN, which was in too great a concentration to meet discharge standards. This problem requires further study.

Behavior of Soluble Microbial Products by the Internal Recycle Rate in MBR Process (MBR공정에서 내부 반송비에 따른 생물대사성분의 거동)

  • Lee, Won-Bae;Cha, Gi-Cheol;Jeong, Tae-Young;Kim, Dong-Jin;Yoo, Ik-Keun
    • Journal of Korean Society on Water Environment
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    • v.21 no.6
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    • pp.602-608
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    • 2005
  • A laboratory-scale experiment was conducted to investigate control of soluble microbial products (SMP) by the internal recycle rate in the submerged membrane separation activated sludge process. The internal recycle rate of the reactor RUN 1 and RUN 2 were 100 % and 200 %, respectively. SMP concentration was rapidly accumulated in the reactor (RUN 1). The variation of accumulated SMP concentration was related to the denitrification rate at the beginning experiment however SMP concentration decreased without correlatively to the denitrification rate during long operation time. The microbial kinetic model was rapidly presented in the both microbial growth and extinction in the reactor (RUN 1). In the SMP kinetic model, Internal recycle rate is the lower, value of UAP and BAP which SMP matter were presented low. The study about development of kinetic model is relatively well adjusted to the experiment exception SMP. In the future, SMP formation equation must be thought that continually research is necessary.

A Study of Optimum Operational Methods for Domestic Wastewater Treatment Plants in Korea (국내 하수종말처리장 최적 운전 방안에 관한 연구)

  • Lee, Byong-hi;Lee, Woung-Woon
    • Journal of Korean Society of Water and Wastewater
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    • v.14 no.1
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    • pp.45-53
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    • 2000
  • The optimum operation of domestic wastewater treatment plant can give influence on operational cost and future expansion in Korea where has 93 operational domestic wastewater treatment plants. Also, the study on optimum operation can reduce operation cost and budget of municipalities whom have responsibility of wastewater treatment plant operation. In this study, 9 domestic wastewater treatment plants which can represent 93 plants in Korea have been selected and operational data are gathered. The collected data are inputted to computer simulator based on IAWQ's Activated Sludge Model No.1 and optimum operation methods are developed. Also, the electric power for aeration which has large portion of overall operation cost are calculated based on design and operation conditions and these are compared. In this study, it was found that design wastewater characteristics are 1.6 times higher than those of daily maximum except for E plant and that estimation methods for design wastewater characteristics have to be improved. Based on computer simulation, we found there is no need to operate all aeration tanks due to weak influent overall power for aeration can be reduced to about 43% of design one.

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Analysis of Microbial Community Structure in River Ecosystem Using Quinone Profiles (Quinone profile를 이용한 하천생태계의 미생물군집구조 해석)

  • Lim, Byung-Ran;Lee, Kisay;Ahn, Kyu-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.5
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    • pp.685-690
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    • 2006
  • The differences in microbial community structures between planktonic microorganism and biofilm in rivers were investigated using respiratory quinone profiles. The compositions of microbial quinone for 4 tributaries of the Kyongan Stream located in/flowing through Yongin City, Gyeonggi-Do were analyzed. Ubiquinone(UQ)-8, UQ-9, menaquinone(MK)-6 and Plastoquinone(PQ)-9 were observed in all samples of planktonic microorganism and biofilm for the sites investigated, Most planktonic microorganism and biofilm had UQ-8(15 to 30%) and PQ-9(over 30%) as the dominant quinone type. These results indicated that oxygenic phototrophic microbes(cyanobacteria and/or eukaryotic phytoplankton) and UQ-8 containing proteobacteria constituted major microbial populations in the river. The quinone concentration in the river waters tested, which reflects the concentration of planktonic microorganisms, increases with increasing DOC. Further research into this is required. The microbial diversities of planktonic microorganism and biofilm calculated based on the composition of all quinones were in the range from 4.2 to 7.5, which was lower than those for activated sludge(ranging from 11 to 14.8) and soils(ranging from 13.4 to 16.8). The use of quinone profile appears to be a useful tool for the analysis of microbial community structure in river.

A Study on the Treatment of Wastewater from the Weight-Reduction Process of Polyester (폴리에스테르 감량가공(減量加工) 폐수(廢水)의 최적(最適) 처리방안(處理方案)에 관한 연구(硏究))

  • Chung, Yoon Jin;Yang, Tae Du;Kim, Woong Ha
    • Journal of Korean Society of Water and Wastewater
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    • v.7 no.1
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    • pp.20-28
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    • 1993
  • The Wastewater from the weight reduction process of polyester is more difficult to be treated biologically than the general wastewater from dyeing and finishing processes in textile industries. Above wastewater shows high pH, high organic strength and wide variation of organic loading. These characteristics are due to TPA and EG resulting from alkaline weight-reduction process and make trouble in the operation of activated sludge process. Therefore, the objective of this study is to develop the pretreatment method for the successful operation of treatment process. For the successful pretreatment process, the wastewater from weight-reduction process should be segregated from other wastewater stream and then acidified with concentrated sulfuric acid to precipitate out TPA from DST solution. At the optimum pH of 2. 2, the initial $COD_{cr}$ 60,000mg/l is reduced to 11,500mg/l and the removal efficiency of $COD_{cr}$ is 81.1%. The required amount of sulfuric acid for pretreatment is not greater than the amount for the the existing neutralization process. Moreover, the supernatant of pretreatment process can be reused in acidification of wastewater.

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The Basic Design Parameters and Effluent Characteristics for Aerobic Fixed Biofilm Reactor (호기성 고정생물막 반응기에서 기초 설계인자와 유출수의 성상)

  • 박태주;송승구
    • KSBB Journal
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    • v.7 no.3
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    • pp.235-245
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    • 1992
  • A number of experiments to treat wastewater of a dye plant were conducted to investigate the basic design parameters and effluent characteristics for aerobic fixed biofilm reactor upon the variation of organic loading rate. The media used for this study were SARAN 4000 D with specific surface area $153m^2/m^3$, and 1000 D with specific surface area $307m^2/m^3$. The influent COD concentration ranged from 1250 to 4080 mg/L. Substrate removal and variation of biomass concentration were observed. At the beginning, the effluent quality was poor but improved as the time passed. However the effluent quality became seriously deteriorated when the media clogged. At influent COD of 1250mg/L, the effluent COD varied little, even if the organic loading rate increased from 0.63 to 2.5kg COD/$m^3$/day. It was also noted that the design parameters for activated sludge process could be applied to an aerobic fixed biofilm process under the experimental conditions.

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Insight into influence of iron addition in membrane bioreactor on gel layer fouling

  • Zhang, Haifeng;Lu, Xin;Yu, Haihuan;Song, Lianfa
    • Membrane and Water Treatment
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    • v.8 no.6
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    • pp.543-551
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    • 2017
  • Membrane fouling in membrane bioreactor (MBR) remains a primary challenge for its wider application. The focus of this study to investigate the influence of iron distribution in activated sludge on gel layer fouling in MBR. Significant reduction in the transmembrane pressure (TMP) rise rates was observed in the presence of iron as result of retarding the gel layer formation time. The spatial distribution of iron had a significant impact on the stratification structure of extracellular polymeric substances (EPS) fractions, such as proteins (PN) and polysaccharides (PS). A mitigation of PN or PS from the supernatant to the EPS inner layers was observed in the presence of iron. Compared with the control reactor, the reduction in PN and PS of the supernatant and lower PN/PS rates of the LB-EPS were beneficial to decrease the membrane fouling potential during the gel layer formation. Consequently, the iron addition managed to control gel layer fouling could be a useful strategy in MBR.

제약폐수 활성슬러지 공정에서 DO농도에 따른 미생물의 활성 변화 측정

  • Mun, Sun-Sik;Lee, Sang-Hun;Sin, Jong-Cheol;Choe, Gwang-Geun;Lee, Sang-Hun;Mun, Heung-Man;Lee, Jin-Won
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.355-358
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    • 2002
  • In this study, we have tried to find the maximum microbial efficiency at the various dissolved oxygen (DO) concentration conditions in the treatment of pharmaceutical wastewater. Experiments of activated sludge process were performed to examine the relation between microbial activity and DO concentration in a continuous bioreactor. The reduction rate of COD (chemical oxygen demand) was low at the DO concentration between 0 ppm and 1.0 ppm, but it went higher and showed maximum between 1.5 ppm and 3.0 ppm. Then the COD reduction rate was quickly decreased above the DO concentration of 3.0 ppm.

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Hydrophilicity and Biodegradability of Polyesteramides (폴리에스테르아미드의 친수성과 생분해성)

  • 김은영;박정희
    • Journal of the Korean Society of Clothing and Textiles
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    • v.25 no.7
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    • pp.1270-1280
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    • 2001
  • In this research of biodegradable polymers, it is essential to investigate the relation between biodegradability and molecular structure such as chemical constitution, hydrophilicity, molecular weight, crystallinity, chain orientation, and so on. It is also expected that hydrophilicity of polymer can affect biodegradability because biodegradation occurs with the help of enzymes and microorganisms. This study is to investigate the effect of hydrophilicity on biodegradability of polyesters. Hydrophilicity was varied by adding 5~30 mol% of amide groups, since amide groups are hydrophilic and used for improving thermal and mechanical properties. Surface energies and nitrogen contents by ESCA were measured to determine their hydrophilicity. The biodegradation was examined in activated sludge, enzyme and natural soil by $CO_2$evolution, TOC, weight loss, and observation through microscopy. The results showed that hydrophilicity of polyesteramide films increased with the addition of amide, PBAD series of shorter methylene units showed maximum hydrophilicity at 15~20 mol% of amide contents, but PBSE exhibited maximum values at 5~15 mol% of amide contents. The biodegradability increased as the hydrophilicty on surface increased. The biodegradation rate of PBAD series was higher than that of PBSE series. Therefore, it can be concluded that the addition of appropriate contents of hydrophile enhanced the biodegradability of aliphatic polyesters as well as their physical properties. Also, the experimental results revealed the relation between hydrophilicity and biodegradability of polyesteramides.

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