• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.119-122
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• 2013

Pollens have been known to possess various biological properties. Therefore, pollens have been extensively used in functional food, folk medicine, and beverage industry to improve human health. This study was conducted to establish the optimized protocol for cytoplasm isolation of bee pollen. Data of biochemical parameters and fatty acid profiles were obtained from pollens of Acorn and Darae(Actinidia arguta). Contents of crude protein and crude fat were 24.1% and 11.8% in Acorn pollen, and those of Darae pollen showed 35.8% and 8.7% in crude protein and crude fat respectively. Also after lyophilizing of Acorn pollen, content of crude protein was increased to 26.5%. Main fatty acids were palmitic acid(C16 : 0), oleic acid(C18 : 1), linoleic acid(C18 : 2) and linolenic acid(C18 : 3) in bee pollen. Linoleic acid(37.3%) was dominant fatty acid in Acorn pollen that is one of essential fatty acids. Linolenic acid(48.3%) was dominant fatty acid in Darae pollen that is a polyunsaturated fatty acid. The proportion of unsaturated fatty acids to total acid content was 73.2% in Acorn and 63.2% in Darae pollen, and especially that of polyunsaturated fatty acids was higher than 55%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.2
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• pp.207-215
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• 2014

To investigate the biological benefits of Korean traditional vegetables, anti-oxidative and anti-inflammatory effects of ethanol extracts from blanched and dried sprouts of evening primrose (Oenothera laciniata, OL) and gooseberry (Actinidia arguta, AA) were measured. Total polyphenol and flavonoid contents of OL were higher than those of AA; OL contained 60.4 mg tannic acid/g dry weight and 31.9 mg rutin/g dry weight, while AA contained 33.0 mg tannic acid/g dry weight and 20.3 mg rutin/g dry weight. The $IC_{50}$ value for DPPH radical scavenging activity was $58.2{\mu}g/mL$ for OL ethanol extract and $122.1{\mu}g/mL$ for AA ethanol extract. The reducing power upon $500{\mu}g/mL$ of ethanol extract treatment was as strong as $52.1{\mu}g$ ascorbate eq./mL for OL and $45.3{\mu}g$ ascorbate eq./mL for AA. Regarding anti-inflammatory effects, inhibition rate against 5-lipoxygenase (LOX) and cyclooxygenase (COX)-2 activities were 29.5% and 79.5% for OL, as well as 11.5% and 39.1% for AA, respectively at a concentration of $250{\mu}g/mL$. Lipopolysaccaride ($1{\mu}g/mL$)-treated RAW 264.7 macrophage cells subjected to OL ethanol extract at various concentrations ($0{\sim}25{\mu}g/mL$) showed significantly reduced synthesis of nitrite oxide (NO), prostaglandin (PG) E2, and IL-6 in a dose-dependent manner without cytotoxicity, although TNF-${\alpha}$ synthesis was not affected. In conclusion, both OL and AA sprouts showed strong antioxidative activity, whereas OL showed very strong anti-inflammatory activity via effective reduction of NO, PGE2, and IL-6 synthesis in LPS-activated macrophage cells.

• Microbiology and Biotechnology Letters
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• v.41 no.3
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• pp.327-334
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• 2013

For the development of hardy kiwi wine, we arranged for the post-maturity of hardy kiwi fruit, treated them with calcium carbonate and a pectinase enzyme complex, investigated the resulting physicochemical properties and conducted a sensory evaluation. The period determined for creating post-maturity in the hardy kiwi fruit was determined as 5 days storage at room temperature following maturity. During this time the yield of fruit juice was increased from 22.1% to 53.5% using 0.1% (v/v) cytolase PCL5 for 2 h at room temperature. 0.1% (w/v) calcium carbonate was also added during the process of aging, for the reduction of the sour taste. The fermentation trial of the hardy kiwi wine was prepared using water (25% or 50%), sugar ($24^{\circ}brix$), 0.1% (w/v) $CaCO_3$, 0.1% (v/v) cytolase PCL5, $K_2S_2O_5$ (200 ppm), and yeast ($1.5{\times}10^7$ cell/ml). Fermentation then occurred for 2 weeks at $20^{\circ}C$. The pH value, total acidity, alcohol, and reducing sugar content of the resulting hardy kiwi wines of 25% (v/w) and 50% (v/w) water, were in a range of pH 3.4-3.7, 1.12-1.21%, 14.3-14.4%, and 15-16 g/l, respectively. Citric acid and fructose constituted the major organic acids and the free sugar of the 25% and 50% hardy kiwi wine, respectively. Volatile flavor components, including 10 kinds of esters, 8 kinds of alcohols, 5 kinds of acids, 3 kinds of others and aldehydes, were determined by GC analysis. The results of sensory evaluation demonstrated that 50% hardy kiwi wine is more palatable than 25% hardy kiwi wine.

• The Korean Journal of Community Living Science
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• v.16 no.4
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• pp.95-102
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• 2005

The present study was performed to investigate the 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging and Proly1 endopeptidase (PEP) inhibitory activities of plant extracts. The whole extract of Fragaria yezoensis inhibited the DPPH radical by $90.4\%$ and the stem of Gingko biloba, Gardenia jasminoides for. grandiflora and Rhododendron yedoensa var. poukhanene, the loaves of Rhododendron mucronulatum var. ciliatum, Gardenia jasminoides for. grandiflora and Corylus sieboldiana var. mandshurice, the fruit of Cornus officinalis, and the root of Gingko biloba showed high DPPH radical scavenging activities. In the case of PEP inhibitory activities, high inhibition was observed in the whole Plant of Fragaria ananassa, Fragaria yezoensis and Hypericum erectum, the stem of Actinidia arguta and Rhododendron yedoensa var. poukhanese, the leaves of Rhododendron yedoensa var. poukhanense and Rosa davurica, the fruit of Cornus officinalis. and the root of Acer okamotoanum. There was significant correlation (P=0.000) between DPPH radical scavenging and prolyl endopeptidase inhibitory activities, thus some of plant extracts such as whole Fragaria yezoensis, fruit of Cornus officinalis had high activities in both DPPH-scavenging and prolyl endopeptidase inhibition. Therefore, it is required to examine the mechanical interaction between DPPH-scavenging and prolyl endopeptidase inhibitory activities and further studying plant extracts with both these activities is desired to develop agents for preventing and treating of Alzheimer's disease.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.5
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• pp.727-733
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• 1995

This stduy was attempted to establish the basic data for evaluating chemical components in the sap from birches(Betula platyphylla Sukatschev, Betula costata Trautv, Betula davurica Pallas), bamboos(Phyllostachys pubescens, Phyllostachys bambusoides, Phyllostachys nigra), Darae(Actinidia arguta). Calcium and potassium in five kinds of mineral detected in the sap were dominant mineral, magnesium, sodium and iron in order and calcium, potassium and magnesium are abundant in the sap from bamboo more than the other sample and the contents were 242.0~422.1mg/L, 793.8~ 2504.1mg/L and 72.6~165.9mg/L, respectively. Free sugars of the sap determined were glucose, fructose and sucrose, but maltose was not detected. The contents of glucose and fructose of the sap from Betula platyphylla Sukatschev(#2) were the highest and 42.1g/L and 36.9g/L, respectively. The detectabel nucleotides and their related compounds were CMP, UMP, GMP, IMP, AMP and hypoxanthine. The total contents of composition amino acids detected from eighteen kinds of the sap were in the range of 2.4~30.4mg%. The major amino acids were taurine, glycine, lysine, alanine and threonine in the sap from birch(#1, #2), glutamic acid and lysine in the sap from Betula costata Trauty(#3) and Betula davurica Pallas(#4), lysine, valine, alanine, serine, tyrosine and glutamic acid in the sap from bamboos, and glutamic acid, leucine, alanine in the sap from Darae.

• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.353-358
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• 2014

In order to obtain the basic data that could be used to evaluate the harvest time of new hardy kiwifruit cultivars (Actinidia arguta 'Saehan', 'Daesung' and 'Chilbo'), the seasonal fruit quality and ripening characteristics of hardy kiwifruit were investigated. Fruit sizes of 'Saehan', 'Daesung' and 'Chilbo' were increased from full bloom to 66 days, 85 days and 78 days, respectively. The growth curve of developing fruit of three cultivars showed double sigmoid. As a result of correlation analysis, the seed number per fruit showed a significant positive correlation with fruit weight (r = 0.94~0.97, p<0.01). Fruit length, width, thickness, weight, soluble solid content and titratable acidity were significantly different among the cultivars. Titratable acidity was increased from full bloom to harvest time and the titratable acidity of 'Saehan', 'Daesung' and 'Chilbo' were 1.77%, 1.22% and 1.37% on havest time, respectively. Optimal harvest time of 'Saehan' was 108 days (23 Sep.) after full bloom, those of 'Daesung' and 'Chilbo' were 92 (9 Sep.) days after full bloom.

• Journal of Korean Society of Forest Science
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• v.79 no.1
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• pp.42-55
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• 1990

The forest communities of this area were studied phytosociologically by the method of Z$\ddot{u}$rich-Montpellier school. 1) Forest vegetation in this area divided into mountain forest(Pinus densiflora-Alnus hirsuta community) and plateau(Basin) forest(Weigela subsessilis-Athyrium yokoscense community). 2) Mountain forest(I. Pinus densiflora-Alnus hirsuta community) divided into I-A. Carpinus laxiflora group, I-B. Acer mono group., I-A. Carpinus laxiflora group is further subdivided into I-A-1. Styrax obassia subgroup, I-A-2. Lindera erythrocarpa subgroup, I-A-3. Typical subgroup, I-A-4. Zanthoxylum schinifolium subgroup. 3) Plateau(Basin) forest(II. Weigela subsessilis-Athyrium yokoscense community.) divided into II-A. Carpinus laxiflora group, II-B. Carpinus cordata-Actinidia arguta group, II-C. Typical group. II-A. Carpinus laxiflora group is further subdivided into II-A-1. Symplocos chinensis for. pilosa subgroup, II-A-2. Typical subgroup. II-A-3. Acer mono subgroup. The flora of vasicula plants distributed in Mt. Kasan area was 105 families 350 genera, 515 species, 87 varieties, 2 subspecies and 12 formae in total 616 taxa (This amount to some 64.7% of the whole plant (952 kinds) in Mt. Palgong nature area.).

• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.25-34
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• 2018

This study was performed to discover the comparison of the understory species composition and the growth velocity of the regeneration seedlings in Pinus densiflora forests being managed by the seed-tree method from 2008 year. The investigation site located in Bonghwa, Gyeongsangbuk-do province was established by eighteen quadrats such as six control ones, six slope ones and 6 concave ones, in which we carried out the vegetation survey and measured annual height growth of each seedlings. As a result of vegetation analysis, the understory vegetation of the seed-tree forest area was mainly composed of shrub plants such as Lespedeza bicolor, Zanthoxylum schinifolium, Securinega suffruticosa, Lespedeza maximowiczii and Stephanandra incisa, herb plants such as Miscanthus sinensis var. purpurascens, Carex humilis var. nana, Spodiopogon sibiricus and Artemisia keiskeana, and vine plants such as Pueraria lobata, Smilax sieboldii, Dioscorea batatas, Actinidia arguta, Vitis amurensis and Rubus crataegifolius. Especially, the vine plants were relatively more imported to the concave site than the control site and the slope site. As a result of measurement of growth velocity, the seedlings of the Pinus densiflora appeared to be 3,175 trees/ha for the non-suppressed trees and 7,842 trees/ha for the suppressed trees. In cases of the concave site, individuals of seedlings were much lower than those on the slope site, probably due to the competition effects of vine plants. Consideringly, silvicultural practices for clearing the vine plants should be inevitably accompanied. Also, the growth velocity of the suppressed and non-suppressed trees has been constantly increasing, those of the non-suppressed trees much higher than the suppressed trees. This represents that the seedlings grow rapidly after the suppressed period with competition to the surrounding plants. Therefore, specific practices such as blush control and vine clear should be carried out at the beginning stage of regeneration in the seed-tree stand.

• Korean Journal of Environment and Ecology
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• v.1 no.1
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• pp.46-65
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• 1987

This study was excuted to measure the user's impact area of the Bukhan Mountain National Park. Eighteen sites of the campsite and the picnic area were sample to measure the impact rating of soil and vegetation. And three belt transects were sampled to analyze soil properties and vegetational change by the user's impact. Also user's psychology was investigated through questionnaires with the visitor and 726 answers were collected from May to June in 1987. The regression equation was significant between the distance and the soil hardness. individual number per 100$m^2$, herb coverage and similarity index from the center of picnic area to the non-use site. The tolerant tree against user's impact are Lespedza maximowiczii. Stephanandra insica, Actinidia arguta Symplocos chinensis for. pilosa and the sensitive trees were Fraxinus rhy chophylla Sorbus alnifolia. and Rhodadendron mucronulatun. The facilities factor and social factor are the most affecting factors to User's psychology by the varimax rotated factor analysis. The carrying capacity of the picnic area was 25$m^2$ per man by the degree of psychological satisfaction.

• Korean Journal of Environment and Ecology
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• v.2 no.1
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• pp.19-36
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• 1988

To investigate edge vegetation structure and edge species in Chiak Mountain National Park, field survey was executed from July to September, 1988. Canonpy drip-line type, cantilevered type and advancing type of edge vegetation were observed on site. The relative importance values of major species in each crown layer were changed along distance from edge to forest interior differently by aspect and present tree layer vegetation. Especially light-oriented edge species as codominant species were observed in Pinus densiflora forest interior at south slope and moisture - oriented species as codominant species were observed with light-oriented species in north edge beside torrential stream. No. of species and individuals by crown layer, species diversities and dissimilarities were decreased according to the distance from edge to forest interior, and edge depth was estimated as 15-20m. Dominant species of edge in shrub and ground layer were different by altitude and topographic locations; valley, ridge, summit and edge species at summit were not observed at other area. Floristic similarities between edge vegetations at different environments were affected by altitude, aspect and topographic location. Frequency classes of edge species were different by aspect, altitude and topographic location. Weigela subscssilis showed high frequency class in all environment conditions and Quercus mongolica, Lindera obtusiloba, Symplocos chinensis for, pilosa, weigela subscssilis, Fraxinus rhynchophylla, Actinidia arguta, Rubus crategifolius. Pinus densiflora, Aralia elata etc, were observed as edge species at all environmental conditions, respectively.