Park, Bong-Wook;Choi, Mun-Jeong;Ryu, Young-Mo;Lee, Sung-Gyoon;Hah, Young-Sool;Kim, Deok-Ryong;Cho, Yeong-Cheol;Kim, Jong-Ryoul;Byun, June-Ho
Maxillofacial Plastic and Reconstructive Surgery
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v.30
no.3
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pp.217-224
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2008
Angiogenesis plays an important role in bone development and postnatal bone fracture repair. Vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptors (VEGFRs) have been thought to be primarily involved in promoting angiogenesis. It is well known that VEGF and its receptors have been reported to play an important role in the regulation of the interaction between angiogenesis and osteogenesis during bone repair processes. Dexamethasone, a potent synthetic glucocorticoid, promotes phenotype markers of osteoblast differentiation, such as ALP and osteocalcin. It stimulates in vitro osteogenesis of human bone marrow osteogenic stromal cells. Dexamethasone has been reported to suppress VEGF gene expression in some cells. However, our previous study demonstrated VEGF quantification increased in a time-dependent manner in periosteal-derived osteogenesis under dexamethasone. So, the purpose of this study was to examine the angiogenic phenotypes in cultured human periosteal-derived cells under high-dose dexamethasone. Periosteal-derived cells were cultured using a technique previously described. After passage 3, the periosteal-derived cells were further cultured for 28 days in an osteogenic inductive culture medium containing ascorbic acid, ${\beta}$-glycerophosphate and high-dose dexamethasone, We evaluated the expression of VEGF isoforms, VEGFR-1, VEGFR-2, and neuropilin-1, ALL VEGF isoforms ($VEGF_{121},\;VEGF_{165},\;VEGF_{189}$, and $VEGF_{206}$) expression was observed by RT-PCR analysis. VEGFR-1, VEGFR-2 and neuropilin-1 expression increased up to day 14, particularly during the early stage of mineralization. Our results suggest the involvement of direct VEGFs/VEGFRs system on periosteal-derived cells during early mineralization phase under high-dose of dexamethasone. These also suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
The effects of Ginseng saponins on the in vitro biosynthesis of prostaglandins were examined in order to identify the role of some Ginseng components on the regulation of arachidonic arid metabolism. The productions of prostaglandin $E_2$ (PG$E_2$), $F_2$ (PGF2), thromboxane $B_2$(TX$B_2$) and 6-ketoprostaglandin Fl (6-Keto-PGF1) from [3Hl-arachidonic acid were evaluatpf by radiochromatographic analysis with rabbit kidney microtome, human platelet homogenate and bovine aortic microsome. The amounts of the total prostaglandins produced by cyclooxygenase activity and malondialdehyde from arachidonic acid didn't show significant changes in the presence of Ginseng saponins. Both of panaxadiol and panaxatriol didn't affect the production of PG$E_2$ while the formations of PG$F_2$( and TX$B_2$( were nearkedly reduced and the production of prostacyclin was increased. The formation of TXBE was reduced by ginsenoside $Rb_2$, Rc, and Re, however the production of 6-Keto-PGF1 was increased dose dependently up to 1 mg/ml. Moreover, platelet aggregations induced by arachidonic acid and U46619 (9.11-methanepoxy PG$H_2$), TX$A_2$ mimetics, were also inhibited by three ginsenosides. The effect of G-Re on prostacyclin synthetase was inhibited by tranylcypromine, prostacyclin synthetase inhibitor. These results suggest that Ginseng saponins may not directly act on cyclooxygenase but affect on the divergent pathway from endoperoxide.
The only law related to airblast and ground vibration control in Korea is the Noise and Vibration Control Act enforced by the Ministry of Environment. But this law mainly deals with the annoyance aspects of noises and vibrations in ordinary human life. Hence, the law defines the safety criteria of ground vibration as the vibration level (VL) of dB(V) unit. The ground vibrations produced from blasting, however, have the unique characteristics that can be shown in shock vibrations, and the duration is also very short compared to the vibrations from machinery, tools or facilities. Hence, vibration regulations for blasting operations usually define the safety criterion as the peak particle velocity (PPV) considering the effect of ground vibrations to structural damage. Notwithstanding, there are several attempts that predict VL from PPV or estimate VL based on the scaled distances (SD; in unit of $m/kg^{1/2}$ or $m/kg^{1/3}$) without considering their frequency spectra. It appears that these attempts are conducted mainly for the purpose of satisfying the law in blasting contracts. But, in principle there could no correlation between peaks of velocity and acceleration over entire frequency spectrum. Therefore, such correlations or estimations should be conducted only for the waves with the same or very similar frequency spectra.
Heat shock proteins (HSPs) are a family of highly conserved proteins playing an important role in the functioning of unstressed and stressed cells. The HSP70 family, the most widely studied of the hsps, is constitutively expressed (hsc70) in unstressed cells and is also induced in response to stressors (hsp70), especially those affecting the protein machinery. The HSP/HSC70 proteins act as molecular chaperones and are crucial for protein functioning, including folding, intracellular localization, regulation, secretion, and protein degradation. Here, we report the identification and characterization of the putative amino acid sequence deduced from one cDNA clone identified as heat shock protein 70. The alignment showed that the putative sequence is 100% identical to the heat shock protein 70 cognate (HSC 70) of olive flounder. The 5'-flanking region sequence (approximately 1 kb) ahead of the hsc70 gene was cloned by genome walking and a putative core promoter region and transcription elements were identified. We characterized the promoter of the olive flounder hsc70 gene by examining the ability of 5'-upstream fragments to drive expression of green fluorescent protein (GFP) in live embryos.
Journal of Fisheries and Marine Sciences Education
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v.13
no.1
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pp.99-116
/
2001
Testing is a procedure used to measure a sample of behaviour in order to discover how well a seafarer performs, usually in comparison with others, or compared with identified performance criteria. In this context It is important that the test, in whatever form it is being used, yields consistent results by being valid, reliable and practical. Whilst we can only take a sample of a person's knowledge or comprehension about topic in this way, testing methods do provide a more reliable estimate of performance than most other observational techniques; unsystematic or irregular observation being too unreliable. The method of examination has been specified in the provisions of Regulation 12 of the Decree of the ship officers act as followings. Other necessary matters for conducting oral and written examinations have been set out by the Minister. But written examination is too shortage of period and small number of question to cover the qualification of each level and oral test is just simple and namely Traditionally, written examinations have been provided as the only means for determining the acquisition of knowledge by the mariner. Typically, the examination formats have taken the format of either an essay or multiple choice examinations. Essay items, used in the vast majority of subject examination(not in Korea), consisted of three basic types: situational, descriptive and computational. The level of certificate being examined determined the number and mix of the type of essay questions selected. Oral question has again been used by assessors of seafarer in a wide variety of contexts. Also, oral questioning is often used when observation of performance is undertaken to ask why a certain action has been taken, or to be broaden the scope of what has been observed. At end, Each techniques have their own advantage and disadvantage, so we have to choose some or all of the following techniques, depending upon the certificate, qualification or job for which the trainee is aiming. But in high lank, we have to use both of essay type and multiple choice and with enough time of oral test at least 30 minutes. Who would be the assessor? According to the STCW Code Section A-I/6, instructors, supervisor and assessors are appropriately qualified for particular types and levels of training or assessment of competence of seafarers either on board or ashore, as required under the Convention, in accordance with the provisions of this section.
Marker pens belong to school things that are controlled by the regulation system called safety confirmation under special act on the safety of products for children with the formaldehyde criteria of 20 mg/kg. With nine marker pens available commercially, formaldehyde in marker pen ink was analyzed by present test standard where marking on a fabric swatch with a pen and extracting the swatch in water and derivatization with Nash reagent followed by UV/Vis spectrophotometeric measurement (Nash-UV/Vis method), giving not detected results or a false positive result in case of a colored water extract. However, the contents of formaldehyde in ink of nine marker pens were determinded to range between 3.2 ~ 93.2 mg/kg with three results above the safety criteria of 20 mg/kg by HPLC/DAD measurements on DNPH derivatives of formaldehyde (DNPH-HPLC/DAD method) in ink dissolved directly in water using an ultrasonic bath. Therefore, the DNPH-HPLC/DAD method with the extraction of ultrasonic dissolving ink in water is proposed as a proper method for analyzing formaldehyde in ink. The proposed method has advantages of lower detection limit and accuracy with colored extracts as well as a simple and fast extraction. The accuracy and precision of this method was estimated to be 90.1 ~ 105.4 % and 0.6 ~ 3.3 %, respectively by spiking tests in the ranges of 20 mg/kg and 40 mg/kg using matrixes such as highlighter pen ink, board marker ink, chalk marker pen ink and painter marker ink.
As part of an effort to leap smart cadastre system by doing rearrangement of various mismatches in the land register, the cadastre renovation project is being recently conducted. In response to this demand, this paper proposes an image-based rapid parcel boundary demarcation plan using the high resolution aerial image with a GSD (Ground Sample Distance) of 5cm that matches to real ground boundary situation in residential area. To review the feasibility and accuracy of this proposed methodology, we compared the accuracy of parcel boundary point and parcel area extracted from the digital stereo plotting on the basis of results of cadastral boundary surveying and land register over the selected two test areas. The comparative accuracy result of all boundary points by digital stereo plotting is satisfied with accuracy requirement according to the criteria of the enforcement regulation of cadastral surveying, whereas it exceeded allowable error of ${\pm}0.07m$, more strictly specified in the Special Act on Cadastral Renovation. And about 20% of the total 70 parcels extracted by digital plotting are out of area tolerance in Jecheon study area, and 10% of the total 19 parcels in Suwon study area. The parcels exceeding accuracy limit are mostly due to the occlusion caused by building roof or eaves, and the obstacles such as trees existing on the boundary. Furthermore, an object identification is impossible in image because of vague boundary reference in case of nonexistence of man-made structures or natural features. Therefore, the utilization of boundary identification stickers is recommended as a solution for these types of land parcel.
Journal of the Korea Institute of Building Construction
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v.17
no.2
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pp.191-197
/
2017
The safety nature of construction industry differs from that of manufacturing sector. For instance, accident risk level dramatically varies at each phase of construction process. Korean Occupational and Health Safety Act has been regulated OHS expense and it contributed reducing accident risk and enhancing safety culture for many years. However, although current regulation guides to use OHS expense proportionate to construction progress, it still allows late use of the expense. This study was conducted for the purpose of presenting methods of estimating a step-by-step OHS expense rate required at each construction phase. In order to do provide proper OHS expense schedule, it analysed accident risk of each construction phase by sorting out 1439 cases of construction site fatality reports, and proposed a method of generating appropriate OHS expense scheme according to its construction work progress characteristics. Both linear and sigmoidal S-curve model were used for the analysis, and the latter generally requires earlier use of OHS expense. By comparing the estimated OHS expense use schedule with current criteria, more than 27%p early use of OHS expense is required for the prevention of accident.
Background: A previous study has shown that Euonymus alatus (EA) has an antidotic activities against inflammation, suggesting possibility that EA can exert this beneficial effects to liver injury by an initial protection against drug-induced hepatocyte demage. The present study was undertaken to evaluate the protective effect of EA-extract on experimentally induced hepatitis in ICR mice and to investigate some mechanisms responsible for its action. Methods: Water EA extract was used in this experiments. The mice received i.p. a dose of 700 mg/kg galactosamine (GalN) together with $5{\mu}g/kg$ of endotoxin (LPS), or received i.v. 12 mg/kg of concanavalin A (Con A). EA (4 mg/mouse) was administrated on day -2, -1 and 0 before induction of liver injury. Liver injury was assessed by measurement of serum alanin amino-transferase (SGPT) levels on 9 hr after GaIN.LPS, or 8 hr after con A administration. Results: Treatment with either GaIN or LPS alone did not cause hepatitis. However, simultaneous administration of GalN and LPS to mice resulted in LPS-dose dependent fulminant hepatitis. GaLN/LPS-induced liver injury was reduced when mice were given EA for 3 days before induction. This preventive effect of Ea was more prominent when EA was given by intraperitoneal route rather then by oral route. Pretreatment of EA or dexamethasone inhibited significantly $TNF{\alpha}$ production in GalL/LPS-injured mice. However, EA-treatment did not influence $TNF{\alpha}$-induced hepatitis in GalN-sensitized mice, suggesting that $TNF{\alpha}$ is likely to act as one of final mediators of endotoxin action and the protective effect of EA might be manifested chiefly by inhibition of endotoxin-induced $TNF{\alpha}$ production, not by blocking the $TNF{\alpha}$-action. Injection of Con A into mice evoked remarkable liver injury in a dose dependent fashion. This liver damage was reduced by EA-pretreatment. Dexamethasone significantly reduced both GalL/LPS-induced and Con A-induced liver damages, showing synergism with EA. However, indomethacin reduced only GalN/ LPS-induced hepatitis, not for Con A-induced hepatitis. Conclusion: These results led to the conclusion that EA may be able to contribute at least in part to prevent the drug-induced hepatotoxicity, and that its anti-hepatitis effects might be manifested directly by modulation of endogenous mediators, such as leukotriese D4, $TNF{\alpha}$ and free radical, and indirectly by regulation of immune mediated responses. Also these results suggested that EA could be developed as a potential antidotic agent.
Park, Sangchan;Cho, Byeongreal;Jeong, Jihye;Lee, Minhee;Kim, Taeyoung;Park, Jongho
Journal of the Korean GEO-environmental Society
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v.9
no.6
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pp.13-19
/
2008
The objectives of this paper are to investigate states of these open dumping landfills for managing, utilizing and stabilization of a municipal solid waste landfill site in environmentally secure conditions. The result of the physical analysis of the landfill showed that it is composed of between 29.72-63.84% organic matter, 32.88% vinyl plastic. The environmental assessment of the landfill site confirmed that the landfill is at a maturation phase due to 0.18 of $BOD_5$/CODcr of leachate. VS and FS of TS Was respectively 30.37%, 32.34% and C/N ratio was 21.8. Surface water around landfill was BOD 10.7 mg/g, SS 37.8 mg/g, E-Coli 31,157(MPN/$100m{\ell}$) and Ground water was $COD_{Mn}$ 1.13-1.38 mg/g, $NO_3-N$ 1.025-4.075 mg/g. Leachat indicated T-P 0.002-0.028 mg/g, $NH_3-N$ 4.0-21.0 mg/g. The soil contamination of around landfill didn't appear as below of the regulation of Soil Environment Conservation Act. The Landfill Gas was $CH_4$ 13.25%, $CO_2$ 6.17%, H2S and CO was not detected. Also Surface Water was not detected $CO_2$, $CH_4$, $H_2S$, CO.
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