• 공업화학
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• 제7권1호
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• pp.83-90
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• 1996

AO-AOT계내에서 S/D의 증가에 따라 흡수 및 형광 spectra의 변화 양상이 감소하다가 증가함을 알 수 있으며 회합 및 해리가 일어남을 알 수 있다. 그리고 온도 상승에 따라 순수한 AO계에서는 흡광도가 증가하나 premicellar영역(S/D=10, 20, 50 및 80)내에서는 흡광도가 오히려 점차 감소한다. 한편 AO-AOT계에 첨가한 염의 농도가 증가함에 따라 metachromasy 소멸도는 증가하나 $NaNO_3$의 경우는 이와 반대로 나타난다. 이러한 염의 양이온에 대한 metachromasy 소멸도의 증가 순서는 $Li^+$, $Na^+$ > $Mg^{2+}$ > $Ca^{2+}$이며, 염의 음이온에 대해서는 $Cl^-$ > $SO_4{^{2-}}$ > $NO_3{^-}$로 나타남을 알 수 있었다.

• 대한치과보철학회지
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• 제44권4호
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• pp.466-476
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• 2006

Purpose : Colonization of denture soft lining materials by Candida albicans can result in clinical problem, and deterioration of the materials. This study aimed to compare the retention and penetration of C. albicans into four denture soft lining materials commonly used. Materials and methods : Four denture soft lining materials (Coe-comfort$^{(R)}$, Coe-soft$^{(R)}$, GC soft liner$^{(R)}$, and Tissue conditioner$^{(R)}$) discs were prepared to glass slide and dental stone. Adherence of yeast to surfaces was monitored after one hour incubation of standardized washed cell suspension with test disc surfaces. Adherent cells stained with acridine orange were counted fluorescence microscopy. Penetration of yeast into materials bonded with acrylic resin after 1, 2, 3,4, 5, 6 and 7 days incubation was observed through sections stained using acridine orange and estimated to quantitative analysis using radioisotope. Results : There was statistical significance in cell numbers between smooth and rough surfaces(p<0.05). Higher numbers of cells were observed on rough surfaces. There was statistical significance in adherent cell numbers into smooth and rough surfaces individually(p<0.05). According to the increase of incubation periods, the cells penetrated into denture soft lining materials were shown to increase. The differences among all kinds of soft liner were statistically significant(p<0.05),and the largest number of cells penetrated into soft liners was observed in the Coe-soft$^{(R)}$. Conclusion : Initial adherence and penetration of yeast into denture soft lining materials has been influenced by surface roughness and chemical composition of them. The selection of appropriate materials and their fabrication may promote clinical performance.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1993년도 제27차 학술대회 초록집
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• pp.98-98
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• 1993

편도선의 염증반응은 세균이 편도상피세포에 부착되는 것으로부터 시작되며 부착된 세균은 증식하여 집락을 형성한 후 독소를 분비하여 점막 장벽을 뚫고 조직에 손상을 일으킨다. 저자들은 급성편도선염의 병태를 이해하기 위하여 생체내에서 급성편도선염 환자군과 정상인에서 상피세포의 세균부착성에 대한 차이를 알아보고자 본 실험을 시행하였다. 급욍편도선염 환자군 20례와 정상인 대조군 20례를 대상으로 편도선부위를 면봉으로 문지른후 세포혼합물을 Acridine orange로 염색하여 형광현미경하에서 관찰하였다. 50개의 편도상피세포에서 부착된 세균수를 계산하였다. 또한 동시에 근배양을 시행하였다. 급성편도선염군은 대조군보다 상피세포에서 10% 이상 부착된 세균수가 많았으며 (p<0.05), 상피세포에 부착된 세균수는 연령과 유의한 상관관계를 보여주었다.

• 대한수의학회지
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• 제33권4호
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• pp.665-671
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• 1993

The rate of 67 neonatal calves's infection of Theileria sergenti was investigated in random samples on the farms located in Kyeongki, Chonbuk and Jeju districts of Korea. The criteria used in verifying infection with T sergenti included the detection of parasites by giemsa's stain and acridine orange stain in the blood smear slides. Further evidence of current or previous exposure to T sergenti was based on the demonstration of T sergenti-specific antibody and antigen by the western immunoblot and the indirect immunofluorescence antibody test in the peripheral blood of the calves. The prevalence rates were 35%, 50% and 100% in Kyeongki, Chonbuk and Jeju provinces respectively and the overall prevalence in all the farms was 43.2% by means of acridine orange stain. The parasites that were observed in the peripheral blood of calves was shown surely by the western immunoblot to the characteristic 34KD antigen among the proteins of T sergenti (Korean Isolate). And the antibody of the neonatal calves reacted at the very highest titer(1 : >2,520). These data highlight the significance of T sergenti in the neonatal calf disease in Korea.

• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.255-258
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• 2002

In order to determine the safety of chemicals and pharmaceutical products, various methods can be used to evaluate the toxicity. In this study the genotoxic effect of the widely used industrial chemical, cadmium chloride, was assessed using the micronucleus test in peripheral blood of mice. The presence of micronucleated reticulocytes by microscopic observation following acridine orange staining indicated a potential genotoxic effect. The genotoxicity of intraperitoneally (i.p.) administered cadmium chloride (0.5, 1, 2 mg/kg) appeared to be dose dependent, with the maximum tolerated dose (MTD) found to be 2 mg/kg. Compared to the negative control (saline), cadmium chloride (2 mg/kg) exhibited statistically significant genotoxic potential (P<0.05) but was found to be less than the positive control of mitomycin C (0.5 mg/kg) and was not statistically significant compared to historical negative controls (P>0.05).

• Journal of Microbiology and Biotechnology
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• 제19권8호
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• pp.787-791
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• 2009

In the course of screening for substances that inhibit etoposide (10 ${\mu}g$/ml)-induced apoptosis in human leukemia U937 cells, fungal strain F000120, which exhibits potent inhibitory activity, was selected. The active compound was purified from an ethyl acetate extract of the microorganism by Sep-pak $C_{18}$ column chromatography and HPLC, and was identified as heptelidic acid (koningic acid) by spectroscopic methods. This compound inhibited caspase-3 induction in U937 cells with an $IC_{50}$ value of 40 ${\mu}M$ after 8 h of etoposide treatment. Fluorescent dye staining with acridine orange and ethidium bromide showed that heptelidic acid inhibited apoptosis. Furthermore, it was found that DNA fragmentation and caspase-3 activation, the biological hallmarks of apoptosis, were inhibited by the compound in a dose-dependent manner, suggesting that heptelidic acid inhibits etoposide-induced apoptosis via downregulation of caspases.

• 대한수의학회지
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• 제34권2호
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• pp.417-419
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• 1994

최근 우리나라는 anaplasmosis 유행 지역으로부터 사슴을 비롯한 반추수를 수입하고 있다. 이처럼 리켓치아성 질환을 비롯한 원충성 질병의 국내 유입 기회가 높아지고 있다. 그러나 사슴에서의 anaplasmosis에 대한 연구보고 예는 접할 수 없었다. 1993년 전라북도의 한 사슴 목장에서는 호주 등지로부터 수입한 250 여두의 사슴에서 빈혈을 수반한 심한 쇄약증세를 나타내는 20마리 사슴의 혈액도말 표본을 Giemsa stain 과 acridine orange stain 방법으로 진단하였던 바, 이 중 8 마리에서 Anaplasma spp가 관찰되었기에 anaplasmosis에 의한 경제적 손실을 최소화하는데 일익을 도모하고자 사슴에서의 anaplasmosis 발병을 보고하는 바이다.

• Journal of Microbiology
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• 제34권1호
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• pp.1-6
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• 1996

The nucleic acid of Synechococcus sp. cyanophage was identified as double-stranded DNA by the result of digestion with enzymes such as exonucleases, DNase, and S1 nuclease, and by acridine orange staining. The cyanophage DNA was cleaved with several restriciton ehdonucleases such as ApaI, BamHI, Bg/II, HaeIII, Eco RI, HindIII, PstI, AND aPAI gave the clearest sets of bands on agarose gels and the fragment numbers for each were 12, 20, 29, 20, and 7, respectively. The sums of the size from Bam HI and PstI digestions were estimated approximately 227$\pm$4 kb, which are in agreement with the result of the pulsed field gel electrphoresis. This virus is thought to have the largest genosome among those of known cyanophages, which corresponds to the largest haed ot 90 nm when compared with the head sizes of cyanophages discovered since 1963.

• 한국치위생학회지
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• 제14권2호
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• pp.287-292
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• 2014

Objectives : The purpose of the study is to examine the cell growth effect and autophagy effect of Scutellariae Radix by ethanol extract in SCC 25 cells. Methods : Cell growth inhibitory effect and autophagy induced by Scutellariae Radix were confirmed by WST-1 assay, monodansylcadaverine(MDC) stain, and flow cytometry by acridine orange(AO) stain. Results : The Scutellariae Radix treatment decreased the cell proliferation in a dose and time dependent manner. Scutellariae Radix has anticancer effects that autophagic vacuoles were apparent by MDC and AO staining in SCC 25 cells. Conclusions : Scutellariae Radix showed anticancer activity against SCC 25 cells via autophagy. The data provided the possibility that Scutellariae Radix may potentially contribute to oral cancer treatment.

• Archives of Pharmacal Research
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• 제10권3호
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• pp.153-157
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• 1987

Reaction of oxazolin-5-ones 1 with p-amino-diphenylamine was resulted in the formation of the corresponding imidazoline-5-ones 2 which on treatment with sulphur afforded phenothizaines 3. Acridine derivatives 5 were obtained from acetylaminodiphenyl amines derivatives 6 on heating with AnCl$_2$ at $200^{\circ}C.$3 reacted with chloroacetyl chloride to give 7 which reacted in turn with different amines to give 8. Antibacterial activity of the obtained products was studied.