• Asian-Australasian Journal of Animal Sciences
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• v.25 no.4
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• pp.577-583
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• 2012

This study was carried out to investigate the supplemental effects of dietary garlic extract (GE) on growth performance of juvenile sterlet sturgeon (Acipenser ruthenus). The first experiment was designed to determine the optimum levels of garlic extract as growth promoter during 10 weeks. Three groups (two replicates/group) of 240 fish with mean body weight of 85 g were fed with diets containing 0 (control), 0.5 and 1.0% of GE. The highest weight gain (%) and feed efficiency (%) were found in fish groups fed with diet containing 0.5% GE. Subsequently, the supplemental effects of dietary GE was studied on growth of juvenile sterlet sturgeon (Acipenser ruthenus) with an average body weight of 59.6 g. Fish cultured in freshwater were randomly allotted to each of 10 tanks (two groups of five replicates, 20 fish/tank) and fed diets with 0.5% GE or without GE (control), respectively, at the level of 2.0% of fish body weight per day for 5 weeks. Weight gain (51.1%), feed efficiency (79.1%), specific growth rate (1.18%) and protein efficiency ratio (1.50) of fish fed 0.5% GE were significantly (p<0.05) higher than those fish fed the control diet. Significantly higher protein (PRE 20.4%) and lipid retention efficiencies (LRE, 74.5%) were also found in 0.5% GE group (p<0.05). The present results suggested that dietary GE could improve growth and feed utilization of juvenile sterlet sturgeons.

• Journal of fish pathology
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• v.25 no.1
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• pp.59-65
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• 2012

Overinflation of cardiac stomach occurred in farmed sterlet sturgeon (Acipenser ruthenus) fingerlings. Affected fish congregated and swam upside-down near the edges of the tanks with abdominal distension. When pressed the dilated abdomen, the fish often exhibited a normal swimming pattern for some time, then resumed their characteristic upside-down swimming patterns. Internally, the fish showed abdominal dropsy and dilated stomach filled with yellowish granular contents. When the contents in the stomach, fish feed, water and excrement in the farm inoculated on Sabouraud destrose agar (SDA) at $25^{\circ}C$ for 48 hr. Candida sp. were isolated except from the feed. Candida sp. in excrement or water in the farm may enter into the stomach with feed and then produce gases in the metabolic process, which in turn may cause the stomach overinflation.

• Korean Journal of Ichthyology
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• v.28 no.3
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• pp.179-185
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• 2016

To develop cultivation technique for Acipenser ruthenus and secure basic taxonomic materials, this study examined egg development and larva morphological development. This study used a couple of male and female broodstork matured in a PVC circle water tank (Ø 5 m) with the water temperature of $16.0{\pm}1.0^{\circ}C$ in June 2005. They were oval opaque and sinking cohesive eggs and the sizes of them were between $3.83{\sim}3.85(3.84{\pm}0.01)mm$ (n=10). As for the egg development, the dry process was adopted with artificial insemination in this study, and an hour after the insemination, the embryo disks were developed, 31 hours after the insemination, they reached the end of gastrula stage, and 82 hours (50%) after the insemination, they were hatched. Right after the hatch, the total lengths of larvae were $10.1{\sim}10.3(10.2{\pm}0.11)mm$ (n=10) with big yolks in the venter. 17 days after the hatch, the total lengths of them were $26.2{\sim}32.4(29.3{\pm}4.39)mm$ (n=10) and in the front of a dorsal fin there were 11 scute scales that started to make tracing development. As 50 days after the hatch, the total lengths were $86.4{\sim}93.1(89.8{\pm}4.71)mm$ (n=10), and fin rays all reached integer, they were moved to the larva apparatus.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.9
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• pp.1303-1310
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• 2014

A 12 week growth study was carried out to investigate the supplemental effects of dietary garlic powder (GP) on growth, feed utilization and whole body composition changes of fingerling sterlet sturgeon Acipenser ruthenus (averaging weight, 5.5 g). Following a 24-h fasting, 540 fish were randomly distributed to each of 18 tanks (30 fish/tank) under a semi-recirculation freshwater system. The GP of 0.5% (GP0.5), 1% (GP1), 1.5% (GP1.5), 2% (GP2) and 3% (GP3) was added to the control diet (GP0) containing 43% protein and 16% lipid. After the feeding trial, weight gain (WG) of fish fed GP1.5, GP2 and GP3 were significantly higher (p<0.05) than those of fish fed GP0, GP0.5 and GP1. Feed efficiency and specific growth rate (SGR) showed a similar trend to WG. Protein efficiency ratio of fish fed GP1.5, GP2, and GP3 were significantly higher (p<0.05) than those of fish groups fed the other diets. A significant difference (p<0.05) was found in whole body composition (moisture, crude protein, crude lipid, ash, and fiber) of fish at the end of the experiment. Significantly higher (p<0.05) protein and lipid retention efficiencies (PRE and LRE) were also found in GP1.5, GP2, and GP3 groups. Broken-line regression model analysis and second order polynomial regression model analysis relation on the basis of SGR and WG indicated that the dietary optimal GP level could be greater than 1.77% and 1.79%, but less than 2.95% and 3.18% in fingerling sterlet sturgeon. The present study suggested that dietary GP for fingerling sterlet sturgeon could positively affect growth performance and protein retention.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.314-315
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• 2006

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.10
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• pp.1419-1429
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• 2012

A series of studies were carried out to investigate the supplemental effects of dietary garlic extracts (GE) on whole body amino acids, whole body and muscle free amino acids, fatty acid composition and blood plasma changes in 6 month old juvenile sterlet sturgeon (Acipenser ruthenus). In the first experiment, fish with an average body weight of 59.6 g were randomly allotted to each of 10 tanks (two groups of five replicates, 20 fish/tank) and fed diets with (0.5%) or without (control) GE respectively, at the level of 2% of fish body weight per day for 5 wks. Whole body amino acid composition between the GE and control groups were not different (p>0.05). Among free amino acids in muscle, L-glutamic acid, L-alanine, L-valine, L-leucine and L-phenylalanine were significantly (p<0.05) higher in GE than in control. However, total whole body free amino acids were significantly lower in GE than in control (p<0.05). GE group showed higher EPA (C22:6n3) and DHA (C22:5n3) in their whole body than the other group (p<0.05). In the second experiment, the effects of dietary garlic extracts on blood plasma changes were investigated using 6 month old juvenile sterlet sturgeon averaging 56.5 g. Fish were randomly allotted to each of 2 tanks (300 fish/tank) and fed diets with (0.5%) or without (control) GE respectively, at the rate of 2% of body weight per day for 23 d. At the end of the feeding trial, blood was taken from the tail vein (n = 5, per group) at 1, 12, and 24 h after feeding, respectively. Blood plasma glucose, insulin and the other serological characteristics were also measured to assess postprandial status of the fish. Plasma glucose concentrations (mg/dl) between two groups (GE vs control) were significantly (p< 0.05) different at 1 (50.8 vs 62.4) and 24 h (57.6 vs 73.6) after feeding, respectively, while no significant difference (p>0.05) were noticed at 12 h (74.6 vs 73.0). Plasma insulin concentrations (${\mu}IU$/ml) between the two groups were significantly (p<0.05) different at 1 (10.56 vs 5.06) and 24 h (32.56 vs 2.96) after feeding. The present results suggested that dietary garlic extracts could increase dietary glucose utilization through the insulin secretion, which result in improved fish body quality and feed utilization by juvenile sterlet sturgeon.