• 대한화학회지
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• 제18권2호
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• pp.132-137
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• 1974

酸性 mucopolysaccharide는 鯨胎兒의 nucleus pulposus에서 分離하였다. Gel electophoresis에 있어서 酸性 mucopolysaccharide의 分離는 $0.03{\%}$의 hexamine cobaltic chloride 를 含有하는 0.05M 醋酸소-다 緩衝溶液(pH4.8)中에서 가장 좋았다. Spacer gel 上의 mucopolysaccharide 溶液을 $40{\%}$ sucrose 溶液으로 덮었을 때 mucopolysaccharide의 移動度에 變化가 나타나는 것을 觀察하였으며 이 효과는 醋酸소-다 緩衝溶液에 hexamine cobaltic chloride 을 加하면 消失하는 것을 觀察하였다.

• 한국패류학회지
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• 제12권2호
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• pp.109-121
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• 1996

Histochemical experiment was carry out respectively to confirm the properties of the salis (Achatina fulica and Incilaria fruhstorferi). SDS-PAGE was carried out to compare and invertigate the distribution aspects of protein patterns between the two species. Five types(A, B, F, H and I)of gland cells with four neutral mucopolysaccharide cells and one acid mucopolysaccharide cells and one acid mucopolysaccharide cell were observed in acinous of Achatina fulica, while six types were observed in acinous of Incilaria fruhstorferi: ond acid mucopolysaccharide cell(type-A) and four neutral mucopolysaccharide cells(type-B, C, D and F) and one cell that acid mucopolysaccharide is only mimbrane that surrounded granule(type-E). The results are follows:The thpe-A fland cell is commonly observed between the two species. The type-A gland cell in Achatina fulica possesses a nucleus with a developed heterdchromatin, and the cytoplasm was filled with round granules. The granules were surrounded with an uncertain boundary mimbrane and confirmed with neutral mucopolysaccharides, but is confirmed acid mucopolysaccharide in Incilaria fruhstorferi.The type-B gland cell is obwerved in the two species, too. The type-B gland cell in Achatina fulica was round shaped, and included an evenly alrge nucleus. The uncleoplasm included granules that were confirmed in the neutral mucopolysaccharides of the two species. The type-C and D gland cells exist only in Incilaria fruhstorferi, nucleoplasm was well developed heterochromatins. The type-E gland cell appears in the acinous surrounded the salivary gland of Incilaria fruhstorferi. Thdse granules appear irregular irregular shape and size and the cytoplasm is formed in alveolar. The type-F gland cells are commonly observed in the salivary glands of the two species. They are similar with the type-B gland cell, but the granular shape is comparatively small and irregular, and possess the neutral mucos granules. The type-H gland cells are mainly seen in only Achatina, and in nucleus is a well developed heterochromatin. The cytoplasm is filled with round small granules with acid mucopolysaccharide for alcianophilia observed. The type-I cell was small cell with an irregular shape and only observed in the gland cells of Achatina fulica. The heterochromatins were developed in the nucleus and the granules are not observed in cytoplasm.Secretory ducts of saliva are composed of the interlobular duct and interlobar secretory duct. In Achatina fulica the interlobular duct consists of a simple cuboidal epithelium, while the endothelium of intralobar secretory duct of Incilaria fruhstorferi consists of a simple squamous epithelium and in the cytoplasm is filled with granules(type-G secretory cell). A SDS-PAGE was carried out to confirm that the protein band pattern consist of salivary gland. In conclusions, five more bands in Achatina fulica and three bands in Incilaria fruhstorferi were confirmed in MW<29 kDa. one main band coincides comparatively with both and is between 29-45 kDa. There are four main bands in Achatina fulica and two main bands in Incilaria fruhstorferi between 45-66.5 kDa respectively. The bands in Achatina fulica seem more complex than in incilaria fruhstorferi.

• 대한수의학회지
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• 제26권2호
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• pp.211-219
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• 1986

In order to know the comparative histological structure of the tongue of some domestic and laboratory animals, the root of the tongue has been investigated by light microscopy. The filiform papillae were mainly distributed in the root of the tongue of most animals and many foliate papillae were also found in the dog and rabbit. On the other hand, only filiform papillae were found in the fowl. The very well-developed mucosa was found in the cattle and in the dog, fowl, rat and mouse was fair. The lamina propria was well developed in the cattle, dog and pig, The muscle layer was well developed in the cattle. The adipose tissue which distributed between the muscle fibers was mainly found in the dog. The lingual glands were well developed in the pig, fowl and rabbit. The properties of these glands were shown strung acid mucopolysaccharide in the dog and pig, weak acid mucopolysaccharide in the fowl, rabbit and rat and neutral or acid mucopolysaccharide in the mouse. The species differences on the histological structure of the tongue were also ascertained by light microscopical observations.

• 한국패류학회지
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• 제15권2호
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• pp.93-104
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• 1999

A histochemical and ultrastructural study on the salivary gland of a freshwater snail Radix auricularia coreana was conducted .The epithelial cells of the salivary gland are composed of 9 types of cells. Two types out of them work as frame cells supporting the epithelium and the secretory cells embedded within the epithelium Seven types of secretory cells are classifiable depend on their histochemical reactions and ultrastructures. The materials secreted by the secretory cells are neutral mucopolysaccharide, acid mucopolysaccharide, and glycogen.

• 한국어류학회지
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• 제11권2호
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• pp.109-116
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• 1999

한국산 미꾸리 Misgurnus anguillicaudatus의 피부 구조 및 조직화학적 특정을 조사하기 위해 등, 측면, 복부, 머리부분의 피부를 조사하였다. 미꾸리의 epidermis는 elongated mucous cell과 club cell의 2개의 gland로 구성되었으며 두께는 아주 두꺼웠다. mucous cell은 acid mucopolysaccharides의 물질을 분비하였으나 club cell은 mucopolysaccharides에 어떠한 반응도 보이지 않았다. 표피의 기저층은 림파구를 가지는 lymphocytic space가 발달하였으며 기저막과 상피세포사이에 배모양의 감각세포인 pit organ이 존재하였다. Dermis의 stratum laxum에는 acid mucopolysaccharides를 나타내는 부분(a definite area)이 존재하며, 이러한 물질은 비늘에도 분포하고 있다. 작은 비늘이 dermis속에 파 묻혀 있으나 머리부분에는 없다. 또한 많은 양의 모세혈관들이 기저막 바로 아래에 존재하였다. 피부의 이러한 특징들은 미꾸리가 공기를 이용하는 피부호흡과 밀접한 관계가 있다.

• 대한치과의사협회지
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• 제13권1호
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• pp.37-45
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• 1975

• Nutrition Research and Practice
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• 제13권6호
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• pp.480-487
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• 2019

BACKGROUND/OBJECTIVES: Osteoarthritis (OA) is a major public health issue in Japan and other countries, and foods that prevent or treat OA are in strong demand. Proteins and peptides in chicken meat and bones are known for being rich in functional and nutritional ingredients for the improvement of osteoporosis. We speculated that chicken legs, a food consumed in many regions of the world, may also contain such ingredients. In this study, we aim to (i) evaluate the effect of chicken leg extract (CLE) on the promotion of cartilage matrix production and (ii) identify the active ingredient in CLE that contributes to this function. MATERIALS/METHODS: Artificial CLE digest was prepared, and the acid mucopolysaccharide production-promoting activity of the CLE digest was evaluated by alcian blue staining of ATDC5 cells. CLE was orally administered to rabbits with burr holes in the knee joint of the femur, and the degree of regeneration of cartilage matrix was evaluated. Furthermore, we investigated orally administered CLE-derived peptides in human plasma using LC-MS. From measuring the acid mucopolysaccharide production-promotion activity of these peptides, a molecule considered to be an active ingredient in the CLE digest was identified. RESULTS: CLE digest promoted acid mucopolysaccharide production and facilitated regeneration of cartilage matrix in in vitro and in vivo experiments. Four peptides including phenylalanyl-hydroxyproline (Phe-Hyp) were detected as CLE-derived peptides in human plasma. The effect of CLE was inferred to be due to Phe-Hyp, which was confirmed to be present in the CLE digest. CONCLUSIONS: It was shown that CLE stimulated the production of articular cartilage matrix both in vitro and in vivo, and that CLE could be an effective food for preventing or treating OA. Furthermore, only Phe-Hyp was confirmed as the active compound in the CLE digest, suggesting that the activity of CLE was due to Phe-Hyp.

• 한국패류학회지
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• 제20권1호
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• pp.7-16
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• 2004

Histochemical and ultrastructural studies on the salivary gland and salivary duct of a land snail Nesiohelix samarangae were conducted to observe structural characteristics and function. The salivary gland consisted of one type of epithelial cell, one type of supporting cell, and six types of gland cells. Four out of six gland cell types were histochemically identified on these secretions. The one secreted acid mucopolysaccharide and the other three secreted neutral mucopolysaccharide. The salivary duct epithelium had only one type of columnar cell with microvilli on its luminal surface. The basal protoplasmic membranes of the epithelial cells were deeply infolded so many times all along the cell bases.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1992년도 제1회 신약개발 연구발표회 초록집
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• pp.21-21
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• 1992

녹용은 한방에서 신경조절 약화, 신체발육부진, 허약체질 등에 사용되는 강장제로서 인삼과 더불어 중요한 약재 중의 하나이지만 아직까지 유효 성분이 밝혀져 있지 않다. 알려진 녹용의 성분으로 collagen, acid mucopolysaccharide, 중성지질, 당지질, ganglioside, 인지질, proteolipid, prostaglandins, estrone, estradiol, cholesterol, its ester, 7-keto-cholesterol, 7$\alpha$-hydroxycholesterol , 7$\beta$-hydroxycholesterol , p-hydroxy-benzaldehyde, uracil, uridine, hypoxanthine, nicotinic acid, creatinine urea 등이 보고되었다. 이와 같은 성분들은 동물조직에서 공통적으로 발견되는 것들로서 녹용의 특이성분이라 부르기 어렵다. 그러나 uracil, uridine, hypoxanthine과 같은 핵산염기 관련 성분은 녹용에 비교적 함량이 많고, 이중에서 hypoxanthine은 monoamine oxidase-B에 대한 저해작용이 있으므로 주목할 필요성이 있다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2007년도 Proceedings of The Convention
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• pp.140-152
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• 2007

Hyaluronic acid(HA) is a naturally occurring mucopolysaccharide composed of the repeating disaccharide unit of D-glucuronic acid and N-acetyl-D-glucosamine. HA exists as a high molecular weight polymer in the extracellular matrix of many tissues in the body. HA has been widely used not only for osteoarthritis and ophthalmology but also for cosmetics for skin care. Recently HA has drawin much attention for use in health foods. Bioland Corporation started production of HA t1y biotechnological process in 1990 for cosmetical use. At present Bioland produces HA for cosmetic and food use. We have done many researches to evaluate the helpful effect for skin and safety of HA as food. In order to evaluate the effect of HA on the skin after its oral intake, we measured its bioavailability, skin-care effects on anti-wrinkle, moisturization elasticity and desquamation by clinical trial. The results showed that HA is very safe and could bs helpful to improve anti-wrinkling, moisturization, elasticity, and desquamation on the skin.