• 한국식품영양학회지
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• 제7권2호
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• pp.128-136
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• 1994

Mackerel muscle protein hydrolysates, which were prepared from defatted mackerel meal by proteases such as complex enzyme, alcalase, bromelain, pancrease, pepsin, w-chymotrypsin, trypsin and papain, were tested for the antioxidative action against linoleic acid. Among proteases tested, the hydrolysates obtained from the treatment of complex enzyme, bromelain and alcalase showed higher antioxidative effects. Also, the hydrolysates showed the synergistic effects with o-tocopherol and the inhibitory effects for peroxidation of metal ions(Fe3+, Cua+) From the profiles of fractionation of the hydrolysates with Bio-gel P-2 column, the most active fractions, part I(complex enzyme-derived) and part e(bromelain-derived), had below MW 1,400 and the antioxidative effects were closely related to the binding capacity with metal ion(Cua+). Amno acid composition of the part I was abundant in histidine, arginine, phenylalanine and lysine, and the part e was abundant in lysine, glutamic acid and leucine.

• 동아시아식생활학회지
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• 제19권1호
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• pp.45-51
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• 2009

In this study, food protein hydrolysates were prepared from six types of food protein: purified meat protein, whole egg protein, casein, isolated soy protein, concentrated rice protein, and gluten. Food proteins were hydrolyzed with pepsin and ethanol (80%)-soluble fractions of pepsin hydrolysates were employed for analysis. The products were colorless and odorless powders with low fat content and good solubility. The MW (molecular weight) of the protein hydrolysates was confirmed to be $200{\sim}1,800$ via gel filtration. Free amino acid contents accounted for less than 5% of the samples. The results of our amino acid analysis revealed that all food protein hydrolysates preserved their original amino acid compositions and nutritional values of their source proteins with highly pure oligopeptide mixtures. These results show that the food protein hydrolysates prepared in these investigations should prove excellent dietary nitrogen sources for a variety of applications.

• 생명과학회지
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• 제10권5호
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• pp.436-446
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• 2000

This study was conducted to investigate the effects of different dietary enzymatic hydrolysates using squid and mackerel by-product on feed efficiency, growth and chemical composition in Korean rockfish (Sebastes schlegeli) after a eight weeks feeding experiment. Effects on the weight gain and feed efficiency in the enzymatic hydrolysates added groups were very effective. Weight gain were significantly higher in the squid hydrolysates whole added group than in the other groups. Moisture and crude ash contents of muscle were not significantly affected by hydrolysates supplementation in all groups. Crude protein and lipid contents of muscle were increased after eight weeks feeding trial. Amino acid contents of muscle were higher in squid hydrolysates whole added group than in the other groups. Amino acids were not affected by hydrolysates supplementation in all groups. Fatty acid contents of muscle were increased after eight weeks growth trial. Fatty acids were not significantly different in the dietary groups.

• 한국식품영양과학회지
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• 제30권5호
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• pp.1004-1009
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• 2001

누에고치를 2N HCl 용액으로 11$0^{\circ}C$, 48시간 가수분해한후 중화하여 냉동건조하여 얻는 누에고치 산가수분해물의 아미노산 조성은 glycin이 43.25%, alanine이 34.39%, serine 10.05%, valine이 2.44%였고 필수 아미노산은 7.12% 차지하였다. 누에고치 산가수분해물의 사료이용효율은 카제인과 같았다. 1~5% 실크분말의 첨가에 의한 간의 무게, 간효소활성(GOT, GPT), 혈청알부민, 혈청 총단백질의 함량은 영향이 없어 단백질의 질이 낮은 것으로 평가되었다. 흰주에 고콜레스테롤, 고지질, 고설탕사료를 각각 섭취시키면서 5% 누에고치 산가수분해물을 첨가하였을 때 혈장 지질농도에 미치는 영향은 중성지질의 감소, 인지질과 HDL의 증가 등 혈액지질조정에 바람직한 결과를 보였으나, LDL과 총콜레스테롤을 높이는 좋지 못한 효과도 나타냈다. 그러므로 누에고치 산가수분해물은 단백질 급원 식품으로보다는 이를 이용한 펩타이드의 생리기능 또는 식용 이외의 화장품재료, 의약용재료, 포장제 등과 기타 물리 화학적 특성을 기초로 한 다양한 범위의 응용이 더욱 적합하다고 생각되며, 혈액지질 개선효과에 대해서는 심층적인 연구가 필요하다.

• 농업생명과학연구
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• 제50권1호
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• pp.155-165
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• 2016

The study investigated the effects of adding bovine plasma protein(PP) hydrolysates on the quality properties of cooked pork patties. Pork patties were prepared as follows: manufactured with pork back-fat(control); replacement of back-fat with 40% olive oil(T1), 40% olive oil and 2% PP hydrolysates(T2), and 40% olive oil and 4% PP hydrolysates(T3). The olive oil modified the fatty acid profiles of the pork patties by lowering the saturated fatty acids(SFAs) percentage. Olive oil and 4% PP hydrolysates addition reduced the level of 2-thiobarbituric acid-reactive substance(TBARS) values in pork patties, compared to the controls. Furthermore, the pork patties with added PP hydrolysates had higher pH values than the control. All samples containing olive oil and PP hydrolysates had increased levels of DPPH radical scavenging activity. In particular, added PP hydrolysates were more effective in increasing antioxidant activity than were the other treatments. Therefore, PP hydrolysates could be used as a natural antioxidative in cooked pork patties.

• 한국축산식품학회지
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• 제34권2호
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• pp.192-199
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• 2014

The effects of adding mechanically deboned chicken (MDC) hydrolysates on the quality characteristics of imitation crab stick (ICS) during storage were investigated. ICS was prepared from Alaska Pollack, chicken breast surimi, and protein hydrolysates enzymatically extracted from MDC. ICS samples were divided into 4 groups: without protein hydrolysate (control), added with 0.5% protein hydrolysate (T1), added with 1.0% protein hydrolysate (T2), and added with 1.5% protein hydrolysate (T3). Results showed that crude protein content did not differ significantly among the ICS samples (p>0.05). ICS sample added with MDC hydrolysates had higher crude fat and ash content but lower moisture content than the control (p<0.05). Lightness was significantly lower in T2 and T3 than in the other groups at 0 and 4 wk of storage. Also, whiteness decreased in the groups contained MDC hydrolysates. Breaking force and jelly strength were higher in samples containing MDC hydrolysates compared to control samples (p<0.05). Additionally, saturated fatty acid contents were lower in the groups containing MDC hydrolysates than in control sample groups (p<0.05). Polyunsaturated fatty acid (PUFA) and essential fatty acids (EFA) were significantly higher in T2 and T3 than the control samples. In particular, all samples containing MDC hydrolysates had reduced thiobarbituric acid-reactive substances (TBARS) values at 4 wk. Free radical scavenging activity also was increased with addition of MDC hydrolysates.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1479-1484
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• 2022

Bacterial cellulose (BC) is gaining attention as a carbon-neutral alternative to plant cellulose, and as a means to prevent deforestation and achieve a carbon-neutral society. However, the high cost of fermentation media for BC production is a barrier to its industrialization. In this study, chestnut shell (CS) hydrolysates were used as a carbon source for the BC-producing bacteria strain, Gluconacetobacter xylinus ATCC 53524. To evaluate the suitability of the CS hydrolysates, major inhibitors in the hydrolysates were analyzed, and BC production was profiled during fermentation. CS hydrolysates (40 g glucose/l) contained 1.9 g/l acetic acid when applied directly to the main medium. As a result, the BC concentration at 96 h using the control group and CS hydrolysates was 12.5 g/l and 16.7 g/l, respectively (1.3-fold improved). In addition, the surface morphology of BC derived from CS hydrolysates revealed more densely packed nanofibrils than the control group. In the microbial BC production using CS, the hydrolysate had no inhibitory effect during fermentation, suggesting it is a suitable feedstock for a sustainable and eco-friendly biorefinery. To the best of our knowledge, this is the first study to valorize CS by utilizing it in BC production.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.256-267
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• 2019

The use of low-cost substrates represents one key issue to make single cell oil production sustainable. Among low-input crops, Arundo donax L. is a perennial herbaceous rhizomatous grass containing both C5 and C6 carbohydrates. The scope of the present work was to investigate and optimize the production of lipids by the oleaginous yeast Cutaneotrichosporon curvatus from undetoxified lignocellulosic hydrolysates of steam-pretreated A. donax. The growth of C. curvatus was first optimized in synthetic media, similar in terms of sugar concentration to hydrolysates, by applying the response surface methodology (RSM) analysis. Then the bioconversion of undetoxified hydrolysates was investigated. A fed-batch process for the fermentation of A. donax hydrolysates was finally implemented in a 2-L bioreactor. Under optimized conditions, the total lipid content was 64% of the dry cell weight and the lipid yield was 63% of the theoretical. The fatty acid profile of C. curvatus triglycerides contained 27% palmitic acid, 33% oleic acid and 32% linoleic acid. These results proved the potential of lipid production from A. donax, which is particularly important for their consideration as substitutes for vegetable oils in many applications such as biodiesel or bioplastics.

• 한국가금학회지
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• 제36권4호
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• pp.311-316
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• 2009

본 시험은 하여 산란계 사료내 누에고치 산가수분해물 첨가시 생산성, 계란 품질, 혈액 성상에 미치는 영향을 조사하기 위하여 수행하였다. 41주령 Hy-Line Brown 갈색계 240수를 공시하여, 4주간 사양 시험을 실시하였다. 일반 무첨가 사료를 대조구로 하여 SP1(일반 사료+누에고치 산가수분해물 0.05%), SP2(일반 사료+누에고치 산가수분해물 0.1%), SP3 (일반 사료+누에고치 산가수분해물 0.2%)으로 총 4개 처리구였으며, 처리 당 5반복, 반복 당 12수씩 완전 임의 배치하였다. 산란율과 난중에 있어서 전체 시험 기간 동안 누에고치 산가수분해물 첨가 수준에 따라 처리구간의 차이를 보이지 않았다. 난각 특성을 나타내는 난각 강도와 두께는 처리구간에 유의적인 차이를 보이지 않았으나, 난황고와 Haugh unit에 있어서는 누에고치 산가수분해물을 첨가 급여한 처리구가 유의적으로 높게 나타났다(P<0.05). 면역 물질인 IgG 함량과 WBC 함량은 누에고치산가수분해물을 첨가한 SP1, SP2, SP3 처리구가 대조구와 비교하여 유의적으로 높게 나타났다(P<0.05). 누에고치 산가수분해물 첨가 급여는 산란계에서 혈액 내 총 단백질, 알부민 함량에 영향을 미치지 않았다. 그러나 혈청 내 인지질, HDL-cholesterol, LDL-cholesterol, total cholesterol 함량을 높여주었다. 사료 내 누에고치 산가수분해물 0.05% 이상 첨가 급여 시 계란의 신선도를 증가시키고, 산란계의 면역과 지질 대사에도 영향을 미치는 것으로 사료된다.