• Asian-Australasian Journal of Animal Sciences
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• 제29권7호
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• pp.965-970
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• 2016

This study was conducted to determine the effects of freezing and supplementation of molasses (M), lactic acid bacteria (LAB) and LAB+enzyme mixture on chemical and nutritional composition of sunflower silage (SF). Sunflower crops were harvested (at about $29.2%{\pm}1.2%$ dry matter) and half of fresh sunflower was ensiled alone and half was frozen (F) at $-20^{\circ}C$ for 7 days. Silage additives were admixed into frozen SF material. All samples were ensiled in glass jars with six replicates for 90 days. The treatments were as follows: i) positive control (non-frozen and no additives, NF), ii) negative control (frozen, no additives, F), iii) F+5% molasses (FM), iv) F+LAB (1.5 g/tons, Lactobacillus plantarum and Enterococcus faecium, FLAB); v) F+LAB+enzyme (2 g/tons Lactobacillus plantarum and Enterococcus faecium and cellulase and amylase enzymes, FLEN). Freezing silage increased dry matter, crude ash, neutral detergent fiber, and acid detergent lignin. The organic matter, total digestible nutrient, non-fiber carbohydrate, metabolizable energy and in vitro dry matter digestibility were negatively influenced by freezing treatments (p<0.05). In conclusion, freezing sunflower plants prior to ensiling may negatively affect silage quality, while molasses supplementation improved some quality traits of frozen silage. Lactic acid bacteria and LAB+enzyme inoculations did not effectively compensate the negative impacts of freezing on sunflower silage.

• Animal Bioscience
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• 제37권2호
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• pp.240-252
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• 2024

Objective: The aim of this study was to investigate the impact of dietary nicotinic acid (NA) on apparent nutrient digestibility, rumen fermentation, and rumen microbiota in uncastrated Xiangzhong black cattle. Methods: Twenty-one uncastrated Xiangzhong black cattle (385.08±15.20 kg) aged 1.5 years were randomly assigned to the control group (CL, 0 mg/kg NA in concentrate diet), NA1 group (800 mg/kg NA in concentrate diet) and NA2 group (1,200 mg/kg NA in concentrate diet). All animals were fed a 60% concentrate diet and 40% dried rice straw for a 120-day feeding experiment. Results: Supplemental NA not only enhanced the apparent nutrient digestibility of acid detergent fiber (p<0.01), but also elevated the rumen acetate and total volatile fatty acid concentrations (p<0.05). 16S rRNA gene sequencing analysis of rumen microbiota revealed that dietary NA changed the diversity of rumen microbiota (p<0.05) and the abundance of bacterial taxa in the rumen. The relative abundances of eight Erysipelotrichales taxa, five Ruminococcaceae taxa, and five Sphaerochaetales taxa were decreased by dietary NA (p<0.05). However, the relative abundances of two taxa belonging to Roseburia faecis were increased by supplemental 800 mg/kg NA, and the abundances of seven Prevotella taxa, three Paraprevotellaceae taxa, three Bifidobacteriaceae taxa, and two operational taxonomic units annotated to Fibrobacter succinogenes were increased by 1,200 mg/kg NA in diets. Furthermore, the correlation analysis found significant correlations between the concentrations of volatile fatty acids in the rumen and the abundances of bacterial taxa, especially Prevotella. Conclusion: The results from this study suggest that dietary NA plays an important role in regulating apparent digestibility of acid detergent fiber, acetate, total volatile fatty acid concentrations, and the composition of rumen microbiota.

• BMB Reports
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• 제30권2호
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• pp.138-143
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• 1997

In order to assess controversial' proposals concerning the fatty acid-induced uncoupling of mitochondrial oxidative phosphorylation, we investigated the interaction of stearic acid with key mitochondrial proteins and measured the effect of stearic acid on the respiration of cytochrome c oxidase vesicles. Electron paramagnetic resonance spectra of spin-labeled stearic acid clearly demonstrated that cytochrome c oxidase interacts strongly with stearic acid. However, the respiration of detergent-solubilized cytochrome c oxidase was not altered significantly by stearic acid. Surprisingly, adenine nucleotide carrier, which was assumed to bind and translocate fatty acid anions in the Skulachev model of uncoupling, did not bind stearic acid at all. The respiration rate of cytochrome c oxidase vesicles was increased by ~70% in the presence of $20{\mu}m$ stearic acid and this uncoupling was attributed to a simple protonophoric effect of stearic acid.

• Asian-Australasian Journal of Animal Sciences
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• 제33권4호
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• pp.615-622
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• 2020

Objective: The objectives were to investigate correlations between energy digestibility (digestible energy [DE]:gross energy [GE]) and various fiber types including crude fiber (CF), total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF), neutral detergent fiber (NDF), and acid detergent fiber (ADF), and to develop prediction equations for estimating DE in feed ingredients and diets for growing pigs. Methods: A total of 289 data with DE values and chemical composition of feeds from 39 studies were used to develop prediction equations for DE. The equations were validated using values provided by the National Research Council. Results: The DE values in feed ingredients ranged from 2,011 to 4,590 kcal/kg dry matter (DM) and those in diets ranged from 2,801 to 4,203 kcal/kg DM. In feed ingredients, DE:GE was negatively correlated (p<0.001) with NDF (r = -0.84), IDF (r = -0.83), TDF (r = -0.82), ADF (r = -0.78), and CF (r = -0.72). A best-fitting model for DE (kcal/kg) in feed ingredients was: 1,356 + (0.704 × GE, kcal/kg) - (60.3 × ash, %) - (27.7 × NDF, %) with R² = 0.80 and p<0.001. In diets, DE:GE was negatively correlated (p<0.01) with NDF (r = -0.72), IDF (r = -0.61), TDF (r = -0.52), CF (r = -0.45), and ADF (r = -0.34). A best-fitting model for DE (kcal/kg) in diets was: 1,551 + (0.606 × GE, kcal/kg) - (22.1 × ash, %) - (25.6 × NDF, %) with R² = 0.62 and p<0.001. All variables are expressed as DM basis. The equation developed for DE in feed ingredients had greater accuracy than a published equation for DE. Conclusion: All fiber types are reasonably good independent variables for predicting DE of swine feeds. The best-fitting model for predicting DE of feeds employed neutral detergent fiber as an independent variable.

• 공업화학
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• 제29권6호
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• pp.753-758
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• 2018

미생물 발효 생계면활성제인 람노리피드의 계면활성(CMC, 표면장력, 기포력 등)을 측정하였다. 람노리피드는 우수한 표면활성을 나타내었다. 람노리피드가 처방된 세정제도 우수한 표면활성을 나타내었다. 액체세정제의 성능평가 결과 지방산 대비 람노리피드가 사용된 제품의 성능이 미세하게 우세하였다. 그렇지만 기포 프로화일에서는 람노리피드가 처방된 제품의 기포량이 아주 많았으며, 헹굼과정에서 지방산의 억포작용과 같은 헹굼성 보조 작용은 나타나지 않았다. 람노리피드는 생계면활성제로써 주계면활성제 또는 보조계면활성제로써 사용될 수 있을 것이다. 그러나 지방산과 같은 억포제 또는 헹굼 보조 기능은 가지지 않은 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.195-202
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• 2023

Protease is a widely used enzyme particularly in the detergent industry. In this research, we aimed to isolate alkaline protease-producing bacteria for characterization as a laundry detergent additive. The screening of alkaline protease production was investigated on basal medium agar plus 1% skim milk at pH 11, with incubation at 30℃. The highest alkaline protease-producing bacterium was 6BS15-4 strain, identified as Bacillus gibsonii by 16S rRNA gene sequencing. While the optimum pH was 12.0, the strain was stable at pH range 7.0-12.0 when incubated at 45℃ for 60 min. The alkaline protease produced by B. gibsonii 6BS15-4 using dairy effluent was characterized. The optimum temperature was 60℃ and the enzyme was stable at 55℃ when incubated at pH 11.0 for 60 min. Metal ions K⁺, Mg²⁺, Cu²⁺, Na⁺, and Zn²⁺ exhibited a slightly stimulatory effect on enzyme activity. The enzyme retained over 80% of its activity in the presence of Ca²⁺, Ba²⁺, and Mn²⁺. Thiol reagent and ethylenediaminetetraacetic acid did not inhibit the enzyme activity, whereas phenylmethylsulfonyl fluoride significantly inhibited the protease activity. The alkaline protease from B. gibsonii 6BS15-4 demonstrated efficiency in blood stain removal and could therefore be used as a detergent additive, with potential for various other industrial applications.

• 한국초지조사료학회지
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• 제40권3호
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• pp.182-189
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• 2020

This study was conducted to evaluate the effect of lactic acid bacteria (LAB) inoculation to domestically-cultivated Italian ryegrass (IRG) on silage fermentation and in vitro ruminal fermentation. There were six treatments based on the LAB inoculants: 1) no addition of LAB (negative control: NC), additions of 2) commercially-available LAB (positive control: PC), 3) Lactobacillus plantarum (LPL), 4) L. paracasei (LPA), 5) L. acidophilus (LA), and 6) L. pentosus (LPT). All treatments were inoculated at a concentration of 10⁶ CFU/g and ensiled for 3, 7, 21, and 42 days in triplicate and analyzed for nutritive values when ensiling was terminated. Day 42 silage from all treatments were also examined for in vitro ruminal fermentation. After 42 days, LAB-inoculated silages had higher (P<0.05) lactic acid concentration compared to the NC. In terms of nutritive values, the silages treated with LPA, LA, and LPT showed higher (P<0.05) crude protein and lower (P<0.05) neutral detergent fiber and acid detergent fiber content compared to the rest of the treatment. In vitro ruminal dry matter degradability was not affected by LAB addition. However, LAB-treated IRG had shown higher (P<0.05) ammonia-N compared with that of the NC. LPA had shown the highest (P<0.05) volatile fatty acid concentration among the LAB examined. In conclusion, the addition of a single strain of LAB appeared to produce a quality IRG silage compared with the NC and the PC. Among the strains examined, LPA seemed to be superior to the others.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.283-283
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• 2017

The objective of the present study is to analyze the lactic acid bacteria (LAB) effects on rye silage fermentation at different stages. Different stages (Booting, Heading, Flowering, and Late flowering stage) of rye were collected from the National livestock farm, National Institute of Animal Science, South Korea. Rye sample was inculcated with lactic acid bacteria and incubated at the anaerobic condition for three months. The nutrient profile such as crude protein (CP), Acid detergent fibre, Neutral detergent fibre and total digestibility nutrients were increased in both control and LAB inculcated samples at all the stages of rye forage. The pH of rye silage was reduced at both stages by LAB inoculation as compared with control. The lactate content was increased in all stages of rye sample by LAB. The acetate concentration and butyrate was reduced in LAB inoculated rye sample. However, acetate concentration was slightly high in LAB inculcated rye at heading and late flowering stage. The LAB population was greater in LAB inoculated rye sample as compared with control sample. However, the massive population was noted in booting stage of rye than the other stages. It indicates the inoculated LAB is the main reason for increasing fermentation quality in the sample through pH reduction by lactate production. Overall results suggest that the isolated lactic acid bacterium is the potent strain that could be suitable for rye forage fermentation at different stages.

• Asian-Australasian Journal of Animal Sciences
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• 제5권2호
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• pp.249-256
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• 1992

Italian ryegrass (Lolium multiflorum Lam.) was fractionated into leaf nutrient concentrate, fiber and deproteinised juice (DPJ). The fresh and fermented DPJs were concentrated and referred to as fresh deproteinised juice concentrate (FDPJC) and fermented deprotenised juice concentrate (FMTD DPJC). The FDPJC and FMTD DPJC were separately mixed with dried fiber and ensiled. Wilted crop silage and fresh fiber silage were also prepared from the same material crop. The nutritive value of these four silages were compared using four goats by $4{\times}4$ Latin square design. Green crop fractionation resulted lesser amount of crude protein and ash, and higher amount of neurtal detergent fiber, acid detergent fiber, cellulose and hemicellulose in fresh fiber. The pH of fresh fiber silage was lower than that of the other silages. Addition of FDPJC or FMTD DPJC to the dried fiber at ensiling did not improve the silage qualities; but all the silages were satisfactorily preserved. Goats fed these silages showed similar ruminal pH and total volatile fatty acid concentrations. But addition of FMTD DPJC was effective on increasing ruminal acetic acid concentration reducing propionic acid concentration. Ruminal n- and iso- butyric acid concentrations were proportional to that of propionic acid. High ammonia content of the silage containing FMTD DPJC was reflected to the ruminal ammonia concentration, urinary nitrogen excretion and serum urea level of goats. Inclusion of FDPJC or FMTD DPJC added 15 to 25% dry matter to the fiber silages with a little reduction in the digestibilities of most components of the silages.

• 한국유기농업학회지
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• 제19권spc호
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• pp.144-147
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• 2011

The main nutritional problem of sorghum${\times}$sudangrass hybrid (Sorghum bicolor${\times}$Sorghum sudanese) silage is low quality and palatability. This experiment was conducted to evaluate whether organic com grain and crushed rice addition of sorghum${\times}$sudangrass hybrid silage increases forage quality of the silage. The sorghum${\times}$sudangrass hybrid silages with added com grain and crushed rice were similar to conventional com silage in moisture content. However organic sorghum${\times}$sudangrass hybrid silage had low pH values. Silage added with gain and byproduct had higher crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control com silages, while its non-fiber carbohydrate (NFC) and total digestible nutrients (TDN) showed the opposite results. Lactic acid, butyric acid and lactic percentage of total organic acid (L/T) of sorghum${\times}$sudangrass hybrid silages were higher than those of com silage, but acetic acid was higher than control. In vitro dry matter digestibility (IVDMD) of com silage was higher than other sorghum silages. Feed intake of sorghum${\times}$sudangrass hybrid silage added with crushed rice was highest among silages. Therefore, these data indicate that crushed rice and com grain added sorghum${\times}$sudangrass hybrid silage could be recommended as the most effective treatment for increasing silage quality and palatability.