• 제목/요약/키워드: Acetaminophen

검색결과 294건 처리시간 0.03초

Acetaminophen에 의해 손상된 마우스 간세포에서 합마유의 간세포보호 효과 (Hepatoprotective Effect of Forest frog's oviduct oil on Acetaminophen-induced Liver Injury in Mice.)

  • 이장천
    • 대한본초학회지
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    • 제22권1호
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    • pp.1-6
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    • 2007
  • Objectives : The purpose of this study is that the protective effects of habmayou on acetaminophen (AP)-induced hepatotoxicity were investigated in mice. Methods : Before administering AP mice supplied with only water were left alone for 18 hours. after concentration and dissolution in poly ethylglycol AP 400mg per 1kg of mouse weight, we injected AP titrated density with a physiological saline solution into the abdominal cavity of mouse to induce hepatotoxicity. we researched mortality rate and the shape of liver tissue of mouse. Results : Treatment with habmayou (250 mg/kg, p.o.) 0.5 h after AP administration significantly prevented an increase in plasma alanine aminotransferase and aspartate aminotransferase activities and AP-induced hepatic necrosis, and also reduced AP-induced mortality from 46% to 0%. In addition, oral treatment with habmayou significantly prevented AP-induced depletion of glutathione (GSH) contents. However, habmayou treatment, by itself, did not affect hepatic GSH contents. Conclusion : These results show that the hepatoprotective effects of habmayou against AP overdose may be due to its ability to block the bioactivation of AP by regeneration of hepatonecrotic cells.

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아세트아미노펜 독성평가를 위한 μCCA-μGI 디바이스의 개발 (The Design and Fabrication of μCCA-μGI Device for Toxicity Evaluation of Acetaminophen)

  • 장정윤
    • Journal of Pharmaceutical Investigation
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    • 제36권4호
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    • pp.263-269
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    • 2006
  • Deficiencies in the early ADMET(absorption, distribution, metabolism, elimination and toxicity) information on drug candidate extract a significant economic penalty on pharmaceutical firms. Microscale cell culture analogue-microscale gastrointestinal(${\mu}CCA-{\mu}GI$) device using Caco 2, L2 and HEp G2/C3A cells, which mimic metabolic process after absorption occurring in humans was used to investigate the toxicity of the model chemical, acetaminophen(AAP). The toxicity of acetaminophen determined after induction of CYP 1A1/2 in Caco 2 cells was not significant. In a coculture system, although no significant reduction in viability of HEp G2/C3A and L2 cells was found, approximately 5 fold increase in the CYP 1A1/2 activity was observed. These results appear to be related to organ-organ interaction. The oral administration of a drug requires addition of the absorption process through small intestine to the current ${\mu}CCA$ device. Therefore, a perfusion coculture system was employed for the evaluation of the absolution across the small intestine and resulting toxicity in the liver and lung. This system give comprehensive and physiologic information on oral uptake and resulting toxicity as in the body. The current ${\mu}CCA$ device can be used to demonstrate the toxic effect due to organ to organ interaction after oral administration,

인진호탕(茵陳蒿湯)의 조합에 따른 간 보호 효과 (Protective effect of injinhotang and its components on acetaminophen-induced hepatoxicity in rats)

  • 최재우;배창욱;박소영;윤현정;박선동
    • 대한한의학방제학회지
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    • 제13권1호
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    • pp.9-33
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    • 2005
  • Acetaminophen, which causes acute liver min in humans and animals, has made useful inducer of hepatoxicity for studying hepatopreventive drugs. Injinhotang is known as one of the hepatopreventive drugs. However, its mechanism of recovery of hepatoxicity treated with acetaminophen is poorly understood. this study was performed to observe the antioxidative effect of injinhotang extract and its several combination groups. The results were obtained as follows:1. In the study on free radical scavenging effect in vitro(the suppressing effect on peroxidation of linoleic acid on concentration, the scavenging effect of DPPH radical, inhibitory effect of superoxide in xanthine-xanthine oxidase system and the inhibitory effect on lipid peroxidation reaction by hydroxy radical in H2O2-Fe2+system, injinhotang have more effect than its components groups relatively. 2. In the study on antioxidants system in vivo(the level of serum LPO, the level of hepatic LPO, catalase, GSH, GST), only injnhotang has a significant effect. 3. In the study on hepatotoxicity(GOT, GPT, $\gamma$-GTP, ALP, LDH, b ilirubin), only injinhotang has a significant effect. These results suggest that injinhotang has the protective effect on acetaminophen-induced hepatoxicity. The mechanisms of these are supposed to be involved in antioxidant and three drugs' cooperative synergy effect.

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Acetaminophen 유도 간 손상에 대한 주적(酒敵)의 보호 효과 (Protective Effect of Joo-Juk on Acetaminophen-induced Liver Damage in Mouse Model)

  • 김성주;강형섭;신재석;설광화;허진;장선일
    • 대한한의학방제학회지
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    • 제17권2호
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    • pp.123-132
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    • 2009
  • Acetaminophen (AP) is widely used as an over-the-counter analgesic and antipyretic drug. AP-induced hepatotoxicity is a common consequence of AP overdose and may lead to acute liver failure. In this study, we investigated the liver damage in mice using single dose (300 mg/kg) of AP and the possible protective effects of administration (50-200 mg/kg body weight) of Joo-Juk on acetaminophen-induced liver damage in mice. The alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities were determined in the plasma of mice. The effect of Joo-Juk on lipid peroxidation product thiobarbituric reacting substances (TBARS) and some antioxidant enzymes superoxide dismutase (SOD), catalase, d-aminolevulinate dehydratase ($\sigma$-ALA-D) activities, and gluthathione peroxidase (GPx), were also evaluated in the mouse liver homogenate. AP caused liver damage as evident by statistically significant increased in plasma activities of AST and ALT. There were statistically significant losses in the activities of SOD, catalase, $\sigma$-ALA-D, and GPx and an increase in TBARS in the liver of AP-treated group compared with the control group. However, Joo-Juk was able to counteract these effects. These results suggest that Joo-juk can act as hepato-protectant against AP toxicity and is a good candidate for further evaluation as an effective chemotherapeutic agent.

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간수, 기문혈의 당귀 약침자극이 acetaminophen으로 유발된 흰쥐의 손상간에 미치는 영향 (Effects of Angelicae gigantis Radix Aqua-acupuncture at Ganshu(BL18) and Qimen(LR14) on Liver Damage induced by Acetaminophen in Rats)

  • 박경미;문진영;안준철;최미정;남경수;임종국
    • 동국한의학연구소논문집
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    • 제5권
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    • pp.1-13
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    • 1996
  • This study was done in order to investigate the protective effects of A.G.R.(Angelicae gigantis Radix) aqua-acupuncture on acetaminophen induced liver damage in rats. The liver damage was induced by acetaminophen (500mg/kg) injection into the peritoneum. The A.G.R. aqua-acupuncture solution was injected into the corresponding loci to Ganshu($BL_{18}$) and Qimen($LR_{14}$) of human body and a blank locus of the root of tail on four consecutive times at 0, 3, 6, and 12 hours after acetaminophen injection. And the serum GOT, GPT, LDH, ALP activities, total bilirubin, direct bilirubin levels were measured in the rats. The serum GOT, GPT, LDH ALP activities and bilirubin level were decreased comparing with that of a control group in case of A.G.R. aqua-acupuncture treated group, specially Ganshu and Qimen aqua-acupuncture treated groups showed an obvious significant decrease.

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Acetaminophen의 감마선 분해에 대한 산화제 영향 (Effect of Oxidants on Decomposition of Acetaminophen by Gamma Irradiation)

  • 이면주;안영덕;이경훤;이오미;김태훈;정인하;유승호
    • 방사선산업학회지
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    • 제5권4호
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    • pp.359-364
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    • 2011
  • This study was aimed to investigate the effect of oxidants on biodegradability and decomposition of acetaminophen (ACT) by gamma ray. Three kinds of chemical, potassium persulfate, hydrogen peroxide and ferrous sulfate were selected as an oxidant. The absorbed dose was ranged from 0.2 to 10 kGy and the concentration of oxidants was from 0.1 to 10 mM and the initial concentration of acetaminophen was $30mg\;l^{-1}$ in this study. The concentration of ACT was gradually decreased corresponding to the increase of the absorbed dose. However, mineralization of ACT was not occurred by the increased of the absorbed dose. When the 10 mM of oxidants applied to the ACT aqueous solution, the concentration of ACT was rapidly decreased according to absorbed dose and the mineralization was observed in potassium persulfate. Biodegradability of ACT with potassium persulfate was higher than that of ACT without potassium persulfate in lower absorbed dose and decreased according to higher absorbed dose.

화간전이 아세트아미노펜에 의한 간독성에 미치는 영향 (Protective Effect of Whagan-Jeon (huaganjian) on Acetaminophen-induced Hepatotoxicity)

  • 박철수;김기열;이채중;안중환;김종대;남경수
    • 대한한의학회지
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    • 제23권3호
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    • pp.33-42
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    • 2002
  • Objective : This study was performed to investigate the activity of Whagan-Jeon (huaganjian) in protection against acetaminophen (AAP)-induced hepatotoxicity and the possible mechanisms in vivo. Methods : The following were performed : Serum ALT, depletion of hepatic glutathione (GSH) levels, the microsomal p. nitrophenol hydroxylation activity, microsomal aniline hydroxylation activity, genomic DNA fragmentation and its reversal, hepatic glutathione-S-transferase (GST) activity, and hepatic NAD(P)H:quinone oxidoreductase (QR) activity Results : Whagan-Jeon (huaganjian) protected against AAP-inducedhepatotoxicity by the increase of GSH levels, inhibition of P450 2E1-specific metabolic activities, attenuation of hepatic DNA damage, and induction of GST and QR activities in vivo. Conclusions : In conclusion, Whagan-Jeon (huaganjian) was effective in protection against AAP-induced hepatoxicity.

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아세트아미노펜 중독 (Acetaminophen Poisoning)

  • 정성필;김승호;이한식
    • 대한임상독성학회지
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    • 제6권1호
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    • pp.1-8
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    • 2008
  • Acetaminophen (AAP) overdose can result in potentially serious hepatotoxicity. The ingested dose and time from ingestion to presentation are important prognostic factors. Toxic dose in adult is thought to be at least 10 g or 200 mg/kg. However, early management of acute overdose should be guided by the plasma AAP concentration. The antidote for AAP poisoning is N-acetylcysteine (NAC). It provides complete protection against hepatotoxicity if given within 8 h of acute overdose. If the concentration is above the possible toxicity line as predicted by the Rumack-Matthew nomogram, either the 72-hr oral or the 20-hr intravenous NAC regimen should be administered. NAC is also effective if started late in patients with established hepatic failure. This article summarizes the current consensus of clinical assessment and management for acute AAP overdose.

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Recent Updates on Acetaminophen Hepatotoxicity: The Role of Nrf2 in Hepatoprotection

  • Gum, Sang Il;Cho, Min Kyung
    • Toxicological Research
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    • 제29권3호
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    • pp.165-172
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    • 2013
  • Acetaminophen (APAP) known as paracetamol is the main ingredient in Tylenol, which has analgesic and anti-pyretic properties. Inappropriate use of APAP causes major morbidity and mortality secondary to hepatic failure. Overdose of APAP depletes the hepatic glutathione (GSH) rapidly, and the metabolic intermediate leads to hepatocellular death. This article reviews the mechanisms of hepatotoxicity and provides an overview of current research studies. Pharmacokinetics including metabolism (activation and detoxification), subsequent transport (efflux)-facilitating excretion, and some other aspects related to toxicity are discussed. Nuclear factor erythroid 2-related factor 2 (Nrf2)-regulated gene battery plays a critical role in the multiple steps associated with the mitigation of APAP toxicity. The role of Nrf2 as a protective target is described, and potential natural products inhibiting APAP toxicity are outlined. This review provides an update on the mechanism of APAP toxicity and highlights the beneficial role of Nrf2 and specific natural products in hepatoprotection.

마두령(馬兜鈴)의 흰쥐 간염(肝炎) 억제(抑制) 효과(效果)에 관(關)한 실험적(實驗的) 연구(硏究) (Inhibitory effects of Maduryung(Aristolochiae Fructus) on alcohol, acetaminophen and galactosamine induced hepatitis in rats)

  • 박호환;정규만
    • 대한한방소아과학회지
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    • 제9권1호
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    • pp.237-256
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    • 1995
  • Maduryung Extract, one of herbal medicine was tested for inhibitory effect to alcohol, acetaminophen and d-galactosamine induced hepatitis in rats. The results were as follows. 1. Increaced serum GOT, GPT levels by alcohol were significantly decreased by Maduryung extract.(p<0.01) 2. Maduryung extract decreaced GOT value in acetaminophen induced hepatitis and this effect may be due to increace of GSH level in liver tissue.(p<0.05) 3. Repeat administration of Maduryung extract showed inhibitory effect on s-GOT, s-GPT levels in d-galactosamine induced hepatitis. (p<0.05) According to the above results, it seems that Maduryung could be use as drug for alcohol or drug induced hepatitis treatment.

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