The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Protective Effect of Whagan-Jeon (huaganjian) on Acetaminophen-induced Hepatotoxicity

화간전이 아세트아미노펜에 의한 간독성에 미치는 영향

  • 박철수 (동국대학교 한의과대학 내과학교실) ;
  • 김기열 (동국대학교 한의과대학 내과학교실) ;
  • 이채중 (동국대학교 한의과대학 내과학교실) ;
  • 안중환 (동국대학교 한의과대학 내과학교실) ;
  • 김종대 (동국대학교 한의과대학 내과학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실)
  • Published : 2002.09.01
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Abstract

Objective : This study was performed to investigate the activity of Whagan-Jeon (huaganjian) in protection against acetaminophen (AAP)-induced hepatotoxicity and the possible mechanisms in vivo. Methods : The following were performed : Serum ALT, depletion of hepatic glutathione (GSH) levels, the microsomal p. nitrophenol hydroxylation activity, microsomal aniline hydroxylation activity, genomic DNA fragmentation and its reversal, hepatic glutathione-S-transferase (GST) activity, and hepatic NAD(P)H:quinone oxidoreductase (QR) activity Results : Whagan-Jeon (huaganjian) protected against AAP-inducedhepatotoxicity by the increase of GSH levels, inhibition of P450 2E1-specific metabolic activities, attenuation of hepatic DNA damage, and induction of GST and QR activities in vivo. Conclusions : In conclusion, Whagan-Jeon (huaganjian) was effective in protection against AAP-induced hepatoxicity.

Keywords

References

  1. 실용중시의결합내과학 陳可冀
  2. J Int Med Res. v.4 History and usage of paracetamol. Spooner JB;Harvey J.
  3. Chem Res Toxicol. v.6 Cytochrome P450 enzymes involved in acetaminophen activation by rat and human microsomes and their kinetics. Patten CJ;Thomas PE;Guy RL;Lee M, Gonzalez FJ;Guengerich FP.
  4. Biochemistry. v.81 N-acetyl-p-benzo-quinone imine: a cytochrome P-450 mediated oxidation product of acetaminophen. Dahlin DC;Miwa GT;Lu AYH;Nelson SD.
  5. Biochem. Pharmacol. v.28 Varying effects of sulfhydryl nucleophiles on acetaminphen oxidation and sulfhydryl adduct formation. Buckpitt AR, Rollins DE;Mitchell JR.
  6. J Biol Chem. v.260 The toxicity of acetaminophen and N-acetyl-p-benzoquinone imine in isolated hepatocytes is associated with thiol depletion and increased cytosolic Ca$^{2+}$. Moore M;Thor H;Moore G;Nelson S;Moldeus P;Orrenius S.
  7. Arch Biochem Biophys. v.295 Role of cytosolic NAD(P)H quinone oxidoreductase and alcohol dehydrogenase in the reduction of p-nitrosophenol following chronic ethanol ingestion. Hajos AKD;Winston GW.
  8. Gut. v.35 Serious paracetamol poisoning and the results of liver transplantation. Multimer DJ;Atyres RCS;Neuberger JM;Davies MH;Holguin J;Buckcls JAC.
  9. Acetaminophen에 의해 유도된 급성 간독성에 미치는 시호의 효과. 강혜경
  10. Acetaminophen의 간독성에 미치는 강활의 효과 정정숙
  11. Acetaminophen에 의한 간독성에 미치는 금은화의 효과. 정규영
  12. 토복령추출물이 Acetaminophen 간독성에 미치는 효과. 김은주
  13. 귀비탕이 acetaminophen으로 유도된 간중독 백서에 미치는 영향. 배창욱
  14. 景岳全書 張介賓
  15. Clin Chem.1978 v.24 Optimization of methods for aspartate amino-transferase and alanine aminotransferase. Bergmeyer HU;Scheibe P;Wahlefeld AW.
  16. Proc Natl Acad Sci (U.S.A). v.77 Increase of NAD(P)H:quinone reductase by dietary antioxidants:Possible role in protection against carcinogenesis and toxicity. Benson AM;Hunkeler MJ;Talalay P.
  17. Mol Phrmacol. v.29 Hydroxylation of p-nitrophenol by rabbit ethanol inducible cytochrome P-450 isozyme 3a. Koop DR.
  18. Toxicol Appl Phamacol. v.120 The induction of cytochrome P45O 2E1 by nitrogen- and sulfur-containing heterocycles: expression and molecular regulation. Kim SG;Novak RF.
  19. J Pharmacol Exp Ther. v.94 The estimation of acetanilide and its metabolic products, aniline. N-acety1-p-aminophenol and p-aminophenol (free and conjugated)in biological fluids and tissues Brodie BB;Axelrod L.
  20. J Pharmacol Exp Ther. v.236 Ray SD;Sorge CL;Kamendulis L;Corcoran GB. Ca2+ activated DNA fragmentation in dimethyl nitrosamine-induced hepatic necrosis. efects of Ca2+ endonuclease and poly(ADP-ribose)polymerase inhibitors in mice.
  21. J Biol Chem. v.262 Direct protective effect of NAD(P)H:quinone reductase against menadione-induced chemiluminescence of postmito-chondrial fractions of mouse liver Prochaska HJ;Talalay P;Sies H.
  22. Biochem Biophys Acts. v.1035 Increased oxidation of p-nitrophenol and aniline by intact hepatocytes isolated from pyrazole rats. Dicker E;MaHugh T;Cederbaum AL.
  23. Pharmacol Ther. v.45 Purification and characterization of hepatic microsomal cytochrome. P-450. Ryan DE;Levin W.
  24. FASEB J. v.6 Oxidative and reductivc metabolism by cytochrome P450 2E1. Koop DR.
  25. 중의간담병학 王伯祥
  26. 동국대 한의학연구소 논문집 v.3 화간전이 carbon tetrachloride로 유도한 간중독 흰쥐에 미치는 영향 유정원;박선동
  27. FASEB J. v.7 Ca$^{2+}$ antagonists inhibit DNA fragmentation and toxic cell death induced by acetaminophen. Ray SD;Kamendulis L;Gurule MW;Yorkin RD;Corcoran GB.
  28. Annu Rev Pharmarol Toxicol. v.30 Covalent and noncovalent interactions in acute lethal cell injury caused by chemicals. Nelson SD;Pearson PG.
  29. Toxicol Appl Pharmacol v.153 Role of CYP1A2 in the hepatotoxicity of acetaminophen: investigations using CYP1A2 null mice. Tonge RP;Kelly EJ;Bruschi SA;Kalhorn T;Eaton DL;Nebert DW.
  30. Toxicol Appl Pharmacol. v.152 Protection against acetaminophentoxicity in CYP1A2 and CYP2E1 double-null mice. Zaher H;Busters JTM;Ward JM;Bruno MK;Lucas AM;Stern ST
  31. Biochem.Pharmacol. v.36 Species differences in the hepatotoxicity of paracetamol are due to differences in the rate of conversion to its toxic metabolite. Tee LBG;Davies DS;Sedden CE;Boobis AR.
  32. J limmunol Methods. v.65 Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Mosman T.
  33. J Biol Chem. v.257 Catalytic activity of cytochrome P-450 isozyme 3a isolated from liver microsomes of ethanol-treated rabbits. Morgan ET;Koop DR;Coon MJ.
  34. Mol Pharmacol. v.33 Diethyl ether as a substrate for acetone/ethanol-inducible cytochrome P-450 and as an inducer for cytochrome(s) P-450. Brady JF;Lee MJ;Li M;Ishizaki H;Yang CS.
  35. Biochemistry. v.26 Regulation of cytochrome P-450j, a high-aftinity N-njtrosodimethylamine demethylase. in rat hepatic microsomes. Thomas PE;Bandiera S;Mainesl LL;Ryan DE;Levin W.
  36. Cancer Res. v.45 Metabolism of nitrosamines by purified rabbit liver cytochrome P-450 isozymes. Yang CS;Tu YY;Koop DR;Coon MJ.
  37. FEBS Lett v.183 Carbontetrachloride-induced lipid peroxidation dependent on an ethanol-inducible form of rabbit liver microsomal cytochrome P-450. Johansson I;Ingelman-Sundberg M.
  38. Cancer Res. v.48 Benzene metabolism by ethanol-,acetone-,and benzene-inducible cytochrome P-450 (IIE1) in rat and rabbit liver microsomes. Johansson I;Ingelman-Sundberg M.
  39. Drug Metab Rev v.22 Cytochrome P45O IIE1:roles in nitrosamine metabolism and mechanism of regulation. Yang CS;Yoo JSH;Ishizaki H;Hong JY.