• Natural Product Sciences
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• v.26 no.1
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• pp.83-89
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• 2020

Osteoporosis is a worldwide disease leading to significant economic and societal burdens globally. Osteoporosis is caused by unbalanced bone remodeling between the rate of osteoclast bone resorption and osteoblast bone formation. Acer tegmentosum Maxim (AT) is a traditional herbal medicine containing multiple biological activities such as anti-oxidant and anti-inflammatory purposes. However, its role in osteoporosis has not been fully studied. Therefore, we investigated whether AT has a potent inhibitory effect on osteoporosis and its mechanism through a systemic evaluation in ovariectomized (OVX) mice. OVX mice were orally administrated with the AT at doses of 50, 100, and 200 mg/kg for 10 weeks. Histological images and histomorphometry analyses were performed by H&E and Toluidine blue satin, and the expression levels of receptor activator for nuclear factor-kB ligand (RANKL), nuclear factor of activated T cells cytoplasm 1 (NFATc1), c-Fos, and matrix metalloproteinase 9 (MMP9) related to the osteoclast differentiation were investigated using immunohistochemical analysis. Administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Further, administration of AT increased periosteal bone formation in a dose-dependent manner. Meanwhile, AT inhibited not only the expression of NFATc1 and c-Fos, which are two major regulators of osteoclastogenesis but also reduced bone resorbed encoding expression of MMP9 and RANKL. Our results indicated that administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Also AT has the bone protective effect through the suppression of osteoclast and promotion of osteoblast, suggesting that it could be a preventive and therapeutic candidate for anti-osteoporosis.

• Korean Journal of Food Science and Technology
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• v.43 no.6
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• pp.787-790
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• 2011

The objective of this study was to investigate the anticancer effects of Acer tegmentosum M. extracts. HepG2 hepatocarcinoma cells were treated with ethanol, chloroform, ethylacetate, butanol, aqueous fraction and hot water extract. The antiproliferative effect was evaluated by trypan blue exclusion, MTT-based viability assay and morphology. The trypan blue test showed that anticancer effect of the A. tegmentosum M. extracts on HepG2 cells increased gradually in proportion to the increasing concentration of the fractions. The butanol fraction showed the highest anticancer activity against HepG2 cells (p<0.05). The MTT assay indicated that the growth inhibition by the butanol fraction was dose-dependent. These results suggest that A. tegmentosum M. has the potential to inhibit the growth of hepatocarcinoma cells.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.125-132
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• 1999

This study has investigated the growing features and vegetation of Acanthopanax chiisanensis and Acanthopanax koreanum in Mt. Jiri and Halla from Sep. 1997 to Aug. 1998. As to Acanthopanax chiisanensis, it occupied surrounding areas of Nogodan in Mt. Jiri of the height of 1,507m, ImgeolyoungJae of 1,300m and the Mt. Halla. of 1,100m of There were rather high constancy degree with Quercus mongolica, Schizandra chinensis, Hydrangen serrate for. acuminata, Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Actaea asiatica, Thalictrum filamentosum, Abies koreana, Arundinella hirta from the sorts. The common species in Mt. Jiri and Halla are Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Callicarpa japonica, Parthenocissus tricuspidata, Acer mono, Ligularia fischeri, Disporum smilacinum, Viola acuminata Aconitum kusnezofii. On the other hand, concerning of Acanthopanax koreanum community, it possessed the small areas of wedo weoltae village in Chejudo. In this area, a liana e.g. Dioscorea tenuipes, Hedera rhombea, Lygodium japonicum, Akebia quinata, Vitis thunbergii, Dunbaria villosa, Trachelospermum asiaticum var. intermedium, Vitis amurensis, Paederia scandens, lives with Pinux thunbergii, Celtis aurantiaca, Mallotus japonicus and so forth in tree layer and subtree layer. There is artificial change no move in the researching sites, Acanthopanax chiixanensis will be transformed into Abies koreana - Acanthopanax chiisanensis community. Soil characteristics showed the high value such as pH 6.56 and conductivity 0.258mmho/cm in Acanthopanax koreanum community of Mt. Jiri, they also indicated digit as follows: the content of organic matter and total nitrogen are from 25.16 through 25.35% and 7.58 through 9.30mg/g respectably. The soil characteristics at Acanthopanax chiisanensis community in Mt. Jiri and Halla, was high as like pH 5.76, conductivity 0.238mmho/cm, total nitrogen 9.30mg/g, total phosphorus 0.126mg/g in Mt. Halla.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.5
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• pp.753-758
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• 2011

We investigated the effects of ultra filtration (UF) of Goroshoe (Acer mono Max.) sap collected in March and April. The total bacteria and turbidity of the sap collected in April were higher than in March. However, after UF, the turbidity decreased and bacteria were not detected. The total acidity and total sugar of the sap collected in April were lower than that collected in March. After UF, total acidity and total sugar decreased slightly in sap from both of the collection times. The crude ash and mineral content of the sap collected in April were higher than in March. In particular, the calcium and potassium content of the sap collected in April were 166.38 and 29.47 mg/L, respectively, which was much higher than in March. Again, after UF, the crude ash and mineral content were decreased in the sap from both collection times. We concluded that UF of Goroshoe sap increased its quality regardless of collection time.

• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.200-207
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• 2006

The objective of this study was to investigate the site and growth characteristics of Acer pictum var. mono growing in the deciduous stands at Mt. Joongwang area located in Pyungchanggun, Kangwon-do. A. pictum var. mono was mainly distributed at the rather steep slope and aspect facing from northeast to northwest, and altitudes ranging from 1,000 to 1,200m. It grew at relatively deep a soil layer with high moisture and nutrients. Major competing species in the upper stories were Betula costata, Acer mandshuricum, Quercus mongolica, Cornus controversa, and herb plants such as Meehania urtidfolia, Isodon excisus, Astilbe chinensis var. davidii, Dryopteris crassirhizoma and Ainsliaea acerifolia in the floor. The height growth was 0.25m in early time, decreased gradually and sustained growth of lower than 10cm. The diameter growth of A. pictum was under 1mm in early time, but increased to 0.3mm/yr and showed continuous growth of 2mm/yr recently. Annual volume growth showed rapid increased about age 120 and then showed stable growth, so the rotation period of A. pictum is expected more than 200 years without decay of heartwood.

• Korean Journal of Environment and Ecology
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• v.21 no.5
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• pp.390-399
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• 2007

We analyzed the vegetation environment at the stream section of habitat in which Cottus koreanus lives. The plant communities were classified into Fraxinus rhynchophylla community, Acer ginnala-Salix koreensis community, Phragmites japonica-Salix gracilistyla community, Salix gracilistyla community, Phragmites japonica community, Robinia pseudo-acacia community, and Oenanthe javanica-Persicaria thunbergii community. The frequency of Phragmites japonica-Salix gracilistyla community was the most. And also the frequencies of Fraxinus rhynchophylla community and Salix gracilistyla community were high. In stream bank zone and terrace zone, the typically represented vegetation was the Fraxinus rhynchophylla community and Acer ginnala-Salix koreensis community and Phragmites japonica-Salix gracilistyla community, respectively. The rNCD of the top-priority plants applied to restoration of the vegetation in stream bank zone came out by the order of Ulmus davidiana var. japonica, Salix koreensis, Acer ginnala, Salix gracilistyla, and Fraxinus rhynchophylla. The rNCD in terrace zone was arranged by the order of Salix gracilistyla, Phragmites japonica, and Artemisia princeps. The spatial distribution of vegetation was the most greatly influenced by cross-sectional position of the surveyed stream, and this aspect is believed to be due to the habitat's physical stability. Species diversity also obviously makes a great difference depending on their surrounding physical stability.

• Korean Journal of Environment and Ecology
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• v.22 no.2
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• pp.106-112
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• 2008

To investigate the vegetation structure of the mountain ridges ranging from Songchu to Dobong, this research set up 22 plots$(400m^2)$ as survey target areas. As a result of the analysis of woody plant cluster, it was classified as two groups-Quercus mongolica community and Quercus mongolica-Pinus densiflora community. Quercus mongolica was found as a mostly dominant woody plant species in the ridge areas from Songchu to Dobong, while Pinus densiflora, Quercus serrata and Quercus acutissima were mixed up partly in low altitudes. High negative correlations were shown between Quercus mongolica and Quercus serrata, Quercus serrata and Acer pseudosieboldianum, Pinus densiflora and Acer pseudosieboldianum, and relatively high positive correlations were found to exist between Quercus acutissima and Quercus serrata; Quercus mongolica and Rhododendron mucronulatum; Pinus rigid a and Rhododendron schlippenbachii; Sorbus alnifolia and Rhododendron mucronulatum; Pinus rigida and Pinus densiflora; Pinus densiflora and Robinia pseudoacacia; Acer pseudosieboldianum and Styrax obassia; Magnolia sieboldii and Symplocos chinensis. Species diversity index(H') by community at the surveyed areas stayed in the scope of $0.997\sim1.160$, which indexes showed nothing different from the vegetation structure of other national parks.

• Korean Journal of Environment and Ecology
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• v.22 no.2
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• pp.98-105
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• 2008

To investigate the vegetation structure of the region from Bukhansanseong to Insubong in Bukhansan National park, 16 plost$(2500m^2)$ set up with random sampling method were surveyed. Three groups Quercus mongolica-Mixed Broad leaved community, Pinus densiflora-Quercus mongolica community, Quercus mongolica community was classified (or communities were classified) by cluster analysis. Quercus mongolica were found as a major woody plant species in Bukhansan National park region. High positive correlation were proved between Quercus mongolica and Rhododendron; Quercus mongolica and Rhus tricocarpa; Rhododendron mucronulatum and Rhododendron schlippenbachi; Symplocos chinensis and Rhododendron schlippenbachii; Acer pseudosieboldianum and Magnolia sieboldii, and relatively high negative correlation was proved between Fraxinus rhynchophylla and Rhododendron mucronulatum; Juniperus rigid a and Acer pseudosieboldianum; Styrax obassia and Acer pseudosieboldianum. Species diversity(H') of investigated groups were ranged from $1.236\sim1.319$ and it was relatively high compared to those of the ridge area of other national parks.

• Korean Journal of Clinical Pharmacy
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• v.12 no.1
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• pp.22-28
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• 2002

Aceclofenac, 2-[(2',6'-dichlorphenyl)amino]phenylacetoxiacetic acid, is a new nonsteroidal anti-inflammatory drug that belongs to the family of phenylacetic acids. It shows good tolerance and potent analgesic/antiinflammatory properties, and acts on cartilaginous chondriocytes, stimulating their repair mechanism. The purpose of the present study was to evaluate the bioequivalence of two aceclofenac products, $Airtal^{TM}$ tablet (Daewoong Pharmaceutical Co.) and $Acer^{TM}$ capsule (Kyungdong Pharmaceutical Co.), according to the guideliner of Korea Food and Drug Administration (KFDA). The aceclofenac release from the two aceclofenac products in vitro was tested using KP VII Apparatus II method at pH 7.8 dissolution media. Sixteen normal male volunteers, $23.13\pm2.03$ years in age and $66.33\pm7.08$ kg in body weight, were divided into two groups and a randomized $2\times2$ cross-over study was employed. After one tablet or capsule containing 100 mg of aceclofenac was orally administered, blood was taken at predetermined time intervals and the concentrations of aceclofenac in serum were determined using HPLC with UV detector. The dissolution profiles of the two aceclofenac products were very similar at pH 7.8 dissolution media. The pharmacokinetic parameters such as $AUC_t,\;C_{max}\;and\;T_max$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters. The results showed that the differences in $AUC_t,\;C_{max}\;and\;T_{max}$ between two products were $6.50\%,\;-1.06\%\;and\;11.96\%$ respectively, when calculated against the $Airtal^{TM}$ tablet. The powers $(1-\beta)\;for\;AUC_t,\;C_{max}\;were\;89.82\%\;and\;82.84\%$, respectively. Minimum detectable differences $(\Delta)\;at\;\alpha=0.05\;and\;1-\beta=0.8$ were less than $20\%\;(e.g.,\;17.51\%\;and\;19.30\%\;for\;AUC_t,\;C_{max}$, ). The $90\%$ confidence intervals were within $\pm20\%\;(e.g.,\;-3.73\%\sim16.73\%\;and\;-12.34\%\sim10.22\%\;for\;AUC_t,\;C_{max},\;respectively)$. Two parameters met the criteria of KFDA for bioequivalence, indicating that $Acer^{TM}$ capsule is bioequivalent to $Airtal^{TM}$ tablet.

• Journal of Korean Society of Forest Science
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• v.96 no.2
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• pp.227-234
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• 2007

The forest canopy gap has been well known as a substantial process of forest cyclic regeneration and important role in stand structure, dynamics, and biodiversity of the forest ecosystem. Based on 3,600 $5m{\times}5m$ square grids in a 9ha permanent experimental plot, the study was conducted to evaluate the regeneration pattern of woody species by developmental stage {seedlings (<1 m of height), saplingI (>1 m of height, <2 cm of DBH), and saplingII (2 cm$<200m^2$), $201-400m^2$, $400-600m^2$, $601-800m^2$, and $>800m^2$) in the mixed broadleaved-Korean pine forest. The results indicated that the regenerating trees of Populus ussuriensis occurred only in the canopy gap area, considered to be a typical gap-dependent species. The regeneration of Ulmus japonica, Ulmus laciniata, and Maackia amurensis could be generally satisfied with the gap size of $201-600m^2$, Betula costata and Prunus padus with gap size of $401-800m^2$, Picea koraiensis with gap size of $201-800m^2$, Fraxinus mandshurica and Syringa reticulata var. mandshurica with smaller than $800m^2$, respectively. Acer ukurunduense and Acer tegmentosum were likely to have no problem with the gap size to make gap regeneration. Acer mono and Tilia amurensis looked more capable of regenerating in the closed canopy disregarding the upper crown condition. The regeneration of Pinus koraiensis and Abies nephrolepis had no trouble under the canopy condition in less than $800m^2$of gap size. The density of regenerating shrubs was rather high, especially under the closed canopy, considered to be associated with great amount of regeneration production in such shade tolerant species as Lonicera maackii, Corylus mandshurica, Euonymus pauciflorus, and Philadelphus schrenkii under the closed canopy. Pearson correlation coefficient was computed to compare the similarity among non-gap area and five gap size classes by developmental stages for trees and shrubs. The similarity coefficients among closed canopy and the gap size classes were mostly significantly correlated to each other with a few exceptions.