• Title/Summary/Keyword: Accumulation layer

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The Production of Egg Yolk Immurnoglobulin (IgY) Raised against 3T3L-1 Cell Membrane Protein and the Control of Adipocytes Differentiation (3T3L-1세포의 막단백질에 대한 난황면역글로뷸린 (IgY)의 생산과 지방세포의 분화조절작용)

  • 김상윤;황성구;구의섭;고태송
    • Korean Journal of Poultry Science
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    • v.26 no.3
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    • pp.179-188
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    • 1999
  • The present was undertaken to establish a model for the control of adipocytes differentiation by using antibody from egg yolk. The emulsion of membrane protein of 3T3L-1 cell membrane protein with the complete Freund's adjuvant was firstly immunized in layer. Second and third boosting were undertaken with two weeks intervals by injection of the emulsion of the same antigen with the incomplete Freund's adjuvant. After 4 week of the first immunization, eggs were collected and antibody (IgY) was purified from egg yolk. The purity of IgY was 60-98% determined by single radial immunodiffusion (SRID) methods. Titer value of the antibody showed high reactiviy for the preadipocytes membrane protein measured by ELISA. When the IgY was added in the test media containing either 2.5% porcine serum or 10% FBS(control), the differentiation of 3T3L-1 cells and Glycerol-3-phosphate dehydrogenase(GPDH) activities was significantly decreased compared to the control cells(p〈0.05). When mice were subcutaneously injected with IgY raised against membrane protein of 3T3L-1 cells for 3 weeks, adipose tissue mass around ovary was tended to be decreased in female mice compared to those of control mice. It is suggested that a potential for manipulating of lipid accumulation through decrease in 3T3L-1 cell differentiation and fat accumulation in female mice by IgY treatment.

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Slope Stability and Development of Debris Flow Deposit in the Ulleung Basin, East Sea (동해 울릉분지의 사면안정성 및 쇄설류 퇴적체의 발달)

  • Lee, Sun-Jong;Lee, Jeong-Min;Yoo, Dong-Geun;Lee, Go-Eun;Park, Soo-Chul
    • Economic and Environmental Geology
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    • v.50 no.2
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    • pp.129-143
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    • 2017
  • The shallow sediments in the southwestern Ulleung Basin consist of mass flow deposits such as slide/slump and debris flow deposits (DFD), caused by slope failure. These sediments are proven to be important in studying geological disaster and stability of the seafloor. In this paper, we analysised the flow accumulation and slope failure susceptibility of the Ulleung Basin on the basis of multi-beam data, collected in this area. We also studied the distribution pattern and the seismic characteristics of the DFD in the uppermost layer of the Ulleung Basin on the basis of seismic data. The slope susceptibility was calculated as the frequency ratio of each factors including slope, aspect, curvature and stream power index (SPI), which causes the slope failure. These results indicate that the slope failure is frequently to occur in the southern and western continental slope of the Ulleung Basin. The sediment flow (mass flow) caused by the slope failure converges to the north and northwest of the Ulleung Basin. According to the seismic characteristics, the uppermost layer in study area can be divided into four sedimentary unit. These sedimentary units develop from the south and southwest to the north and northwest in association with slope susceptibility and flow accumulation.

The Lipid Accumulation Suppressive Effect of Monostroma nitidum In kidney of Murine with hyperlipidemia induced by Triton WR-1339 (Triton WR-1339 주사에 의한 고지혈증 유발 생쥐 신장에 미치는 참홑파래 추출물의 지방축적 억제 영향)

  • Park, In-Sick;Ahn, Sang-Hyun;Choi, Nan-Hee;Kim, Jin-Tack
    • The Journal of Dong Guk Oriental Medicine
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    • v.6 no.2
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    • pp.87-98
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    • 1998
  • Renal tissues of ICR mouse were intraperitoneally injeced with Monostroma nitidum extract(ME) after Triton WR-1339(TX) injection were observed to investigate the lipid accumulation suppressive effect of ME. The renal tissues were obtained at hour 24, 48, and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swatted as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group and these appeared in convoluted tubule, collecting tubule, and Helen's loop. But the parietal layer of Bowman's capsule was shaped as squamous type that was looked like as normal morphology in ME treated group after TX injection and the lipid blot and cholesterol particle were considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of ME.

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Longitudinal Distribution of Zooplankton at 10.5°N in the Northeastern Pacific (북동태평양 북위 10.5°에서 동물플랑크톤의 경도별 분포 특성)

  • Kang, Jung-Hoon;Cho, Kyu-Hee;Son, Ju-Won;Kim, Woong-Seo
    • Ocean and Polar Research
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    • v.29 no.4
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    • pp.283-295
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    • 2007
  • We investigated the longitudinal variations in zooplankton abundances and their related physicochemical properties at nine stations located between $136^{\circ}W$ and $128^{\circ}W$ at $10.5^{\circ}N$ in the northeastern Pacific in summer 2004. Temperature, salinity, inorganic nutrients, chlorophyll-a (hereafter chl-a) and zooplankton ($>200\;{\mu}m$) were sampled within the depth from the surface to 200 m depth at $1^{\circ}$ longitude intervals. Zooplankton($>200\;{\mu}m$) samples were vertically collected at two depth intervals from surface to 200 m, consisting of surface mixed and lower layers (thermocline$\sim$200 m). Longitudinal distributional pattern of hydrological parameters (especially salinity) was physically influenced by the intensity of westward geostrophic current passage relating to the NEC (North Equatorial Current). Data from the longitudinal survey showed clear zonal distributions in the hydrological parameters(temperature, salinity and nutrients). However, spatial patterns of the chl-a concentrations and zooplankton abundances were mostly independent of the zonal distributions of hydrological parameters. The two peaks of zooplankton abundance in the surface mixed layer were characterized by different controlling factors such as bottom-up control from nutrients to zooplankton ($129^{\circ}W$) and accumulation by increment of friction force and taxonomic interrelationship ($133^{\circ}$ and $134^{\circ}W$). Divergence-related upwelling caused introduction of nutrients into surface waters leading to the increment of chl-a concentration and zooplankton abundances ($129^{\circ}W$). Increased friction force in relation to reduced flow rates of geostrophic currents caused accumulation of zooplankton drifting from eastern stations of study area($133^{\circ}$ and $134^{\circ}W$). Besides, high correlation between immature copepods and carnivorous groups such as chaetognaths and cyclopoids also possibly contributed to the enhanced total abundance of zooplankton in the surface mixed layer (p<0.05). Zooplankton community was divided into three groups (A, B, C) which consecutively included the eastern peak of zooplankton($129^{\circ}W$), the western peak($133^{\circ}$ and $134^{\circ}W$) and high nutrient but low chl-a concentration and zooplankton abundance ($136^{\circ}W$). Moreover, Group B corresponded to the westward movement of low saline waters(<33.6 psu) from 128 to $132^{\circ}W$. In summary, longitudinal distributions of zooplankton community was characterized by three different controlling factors: bottom-up control ($129^{\circ}W$), accumulation by increased friction force and relationships among zooplankton groups ($133^{\circ}$ and $134^{\circ}W$), and mismatch between hydrological parameters and zooplankton in the high nutrient low chlorophyll area ($136^{\circ}W$) during the study period.

Habitat Characteristics and Vegetation Structure of the Evergreen Fern in Jejudo, Korea (제주도의 상록양치식물 자생지 환경특성 및 식생구조에 관한 연구)

  • Bang, Kwang Ja;Kim, Kwang-Du;Kang, Hyun-Kyung;Ju, Jin Hee
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.3
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    • pp.64-72
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    • 2004
  • The research was carried out to define the environmental characteristics and vegetation structure of the evergreen fern habitat in Jejudo. The growth conditions of evergreen fern in various habitats was surveyed, including topographical features, vegetation structure, air temperature, humidity, intensity of light, water content and organic matter content in the soil. In the direction of the native habitat, 70% of research area was located in the southeast-facing slopes, 30% was located in southern slopes. The gradient ranged from $0^{\circ}$ to $30^{\circ}$. Temperature ranged from $16^{\circ}C$ to $28^{\circ}C$, and $22.3^{\circ}C$ was the average. Humidity ranged from 20 to 68%, and 36% was the average. In the native habitat, the highest light intensities reached 60,000 to 80,0001ux, but in general ranged from 300 to 40001ux. Water content in the soil ranged from 32% to 59%, organic matter content ranged from 8 to 13%. Within a unit of 25$m^2$, there were tall-tree layer such as Quercus galuca and Castanopsis cuspidata with a covering of rate 40~80%, a sub-tall-tree layer such as Camellia japonica, Staphylea bumalda and Sambucus williamsii with the covering rate of 3~5%, a shrub layer with the covering rate of 5~20%, and a grass layer with the covering rate of 40~95%. This research provides the basic data about the native habitat environment of the evergreen fern plant. Continuous monitoring and accumulation of data is necessary for the use of evergreen fern as vegetation materials.

Changes of Biomarker in Manila clam, Ruditapes philippinarum Exposed to Lead (납(Pb)에 노출된 바지락, Ruditapes philippinarum의 생물지표 변화)

  • Shin, Yun Kyung;Park, Jung Jun;Lim, Hyun Sig;Lee, Jung Sick
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.7-13
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    • 2013
  • This study was conducted to find out biological response of Manila clam, Ruditapes philippinarum exposed to lead (Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and three lead exposure conditions (0.25, 0.50 and 1.00 mg/l). The results of the study confirmed that lead induces reduction of survival rate and oxygen consumption rate and histopathology of organ structure of the bivalve. Oxygen consumption rate was observed exposure groups lower than control decline by 25%-72%. Histological analysis of organ system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration of connective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestive diverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules, and as the concentration of lead increased the accumulation of lipofuscin increased.

Water Layer in Course of Corrosion of Copper in Humid Air Containing $SO_2$

  • Sasaki, Takeshi;Itoh, Jun;Ohtsuka, Toshiaki
    • Corrosion Science and Technology
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    • v.2 no.2
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    • pp.88-92
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    • 2003
  • The technique for in situ simultaneous measurements of IR-RAS and QCM, which has been developed for investigation of corrosion in gaseous environments, was employed to study the effects of an extremely thin water layer on the corrosion rate. An evaporated copper film on a QCM element was exposed to air containing water vapor and $SO_2$, and time-resolved IR-RAS spectra were measured and mass gains were simultaneously followed with QCM. The tested ranges of relative humidity (RH) and concentration of $SO_2$ were 60% - 90% and 1 - 20 ppm, respectively. On the basis of 2D-IR analysis, the corrosion products were determined to be Chevreul's salt ($CuSO_3Cu_2SO_3{\cdot}2H_2O$) and $CuSO_4{\cdot}5H_2O$. By constructing curves of the relations between band intensities of IR spectra and mass gains of QCM for the corrosion products, the time variations in each product were determined from spectral experiments on copper plates. The thicknesses of physically adsorbed water layers in course of the corrosion process were also determined from water band intensities. The results showed that the thickness of the physically adsorbed water layer increased with increase in RH, and it also increased with increase in accumulation of corrosion products. The latter is probably due to the capillary effect of the corrosion products.

Effect of Channel and Gate Structures on Electrical Characteristics of Oxide Thin-Film Transistors (Channel과 gate 구조에 따른 산화물 박막트랜지스터의 전기적 특성 연구)

  • Kong, Heesung;Cho, Kyoungah;Kim, Jaybum;Lim, Junhyung;Kim, Sangsig
    • Journal of IKEEE
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    • v.26 no.3
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    • pp.500-505
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    • 2022
  • In this study, we designed oxide thin-film transistors (TFTs) with dual gate and tri layered split channels, and investigated the structural effect of the TFTs on the electrical characteristics. The dual gates played a key role in increasing the driving current, and the channel structure of tri layers and split form contributed to the increase in the carrier mobility. The tri layered channels consisting of the a-ITGZO and two ITO layers inserted between the gate dielectric and a-ITGZO led to the increase in the on-current by using ITO layers with high conductivity, and the split channels lowered series resistance of the channels. Compared with the mobility (15 cm2/V·s) of the single gate a-ITGZO TFT, the mobility (134 cm2/V·s) of the dual gate tri-layer split channel TFT was remarkably enhanced by the structural effect.

Wear Progress Model by Impact Fretting in Steam Generator Tube (충격 프레팅에 의한 증기발생기 세관 마모손상 진행모델)

  • Lee, Jeong-Kun;Park, Chi-Yong;Kim, Tae-Ryong;Cho, Sun-Young
    • Proceedings of the KSME Conference
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    • 2007.05a
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    • pp.1684-1689
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    • 2007
  • Fretting wear is one of the important degradation mechanisms of steam generator tubes in the nuclear power plants. Especially, impact fretting wear occurred between steam generator tubes and tube support plates or anti-vibration bar. Various tests have been carried out to investigate the wear mechanisms and to report the wear coefficients. Those are fruitful to get insight for the wear damage of steam generator tubes; however, most wear researches have concentrated on sliding wear of the steam generator tubes, which may not represent the wear loading modes in real plants. In the present work, impact fretting tests of steam generator tube were carried out. A wear progression model for impact-fretting wear has been investigated and proposed. The proposed wear progression model of impact-fretting wear is as follows; oxide film breaking step at the initial stage, and layer formation step, energy accumulation step and finally particle torn out step which is followed by layer formation in the stable impact-fretting progress. The wear coefficient according to the work-rate model has been also compared with one between tube and support.

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An Analysis of Riparian Vegetation Distribution Based on Physical Soil Characteristics and Soil Moisture Content -Focused on the Relationship between Soil Characteristics and Vegetation- (토양의 물리적 특성 및 수분조건에 다른 하반식물의 분포 -토양환경과 식생과의 관계를 중심으로-)

  • 안홍규
    • Journal of the Korean Institute of Landscape Architecture
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    • v.28 no.5
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    • pp.39-47
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    • 2000
  • This study is to investigate the conditions closely related to the establishment of vegetation in the riparian zone: the soil condition, an important factor along with climate and light. Especially, the soil structure of the microtopographical formations in the specific area known as the riparian microtopographical zone investigated. In addition, the effect of the riparian microtopographical features on the ground water level, soil moisture content, and vegetation was studied. The results of this study are as follows; 1) At all sample sites, below the sand layer, a gravel layer is always present. This is the result of past floods. 2) Although Salix koreensis experiences frequent disturbances such as increase in river level and floods, this vegetation establishes itself in the most secure are in the microtopographical zone. 3) The growth of Phragmites japonica is closely related to the underground water level. 4) It is clear that Miscanthus sacchariflorus grows concentrated in dry areas. 5) The soil accumulation conditions differ according to the soil moisture content of each microtopgraphical feature. Accordingly, the moisture content of the soil is clearly different within the microtopographical zone. The continuous and long-term investigation and research on the relation of riparian reproduction and the relevance with location surrounding factors are necessary in the future.

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