• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.388-393
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• 2010

Recently, a number of successful research reports are accumulated to increase the carotenoid level in potato tuber such as $\beta$-carotene, precursor of vitamin A and keto-carotenoid like astaxanthin in which is not synthesized in most plants tissue since it does not contain a specific enzyme to add keto-ring in carotenoid molecule. In particular, keto-carotenoids are more interested due to their strong antioxidant activity. Currently, the content of $\beta$-carotene was increased up to 3,600-fold ($47\;{\mu}g/g$ dry weight) when compared to the control potato tuber, parental cultivar for genetic modification. In addition, astaxanthin, one of the major keto-carotenoid was accumulated up to $14\;{\mu}g/g$ dry weight in potato tuber with red color by over expressing the gene encoding $\beta$-carotene ketolase isolated from marine microorganisms. In this article, we summarized carotenogenesis-related genes that have been used for metabolic engineering of carotenoid biosynthetic pathway in potato. Furthermore, strategies for the accumulation of carotenoids and ketocarotenoids in specific potato tuber, bottle necks, and future works are discussed.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.3
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• pp.196-200
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• 2004

The supra molecular weight poly(〔R〕-3-hydroxybutyrate) (PH B), having a molecular weight greater than 2 million Da, has recently been found to possess improved mechanical properties compared with the normal molecular weight PHB, which has a molecular weight of less than 1 million Da. However, applications for this PHB have been hampered due to the difficulty of its production. Reported here, is the development of a new metabolically engineered Escherichia coli strain and its fermentation for high level production of supra molecular weight PHB. Recombinant E. coli strains, harboring plasm ids of different copy numbers containing the Alcaligenes latus PHB biosynthesis genes, were cultured and the molecular weights of the accumulated PHB were compared. When the recombinant E. coli XL1-Blue, harboring a medium-copy-number pJC2 containing the A. latus PHB biosynthesis genes, was cultivated by fed-batch culture at pH 6.0, supra molecular weight PHB could be produced at up to 89.8 g/L with a productivity of 2.07 g PHB/L-h. The molecular weight of PHB obtained under these conditions was as high as 22 MDa, exceeding by an order of magnitude the molecular weight of PHB typically produced in Ralstonia eutropha or recombinant E. coli.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.10
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• pp.1865-1867
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• 2007

We propse a novel corner detection algorithm for locating 2D Data Matrix barcode in an image. The proposed method accumulates weight for each cross point defined by every combination of edge points in the image, and detects the corner point of the barcode L-pattern by determining the location of the highest accumulated weight. By designing the weight considering the connectivity of two lines around the cross point, we were able to detect the corner of L-pattern even for the cases that the lines of L-patterns are short. In the experiments, the proposed method showed improved performance compared with the conventional Hough transform based method in terms of detectability and computation time.

• Journal of Bio-Environment Control
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• v.13 no.2
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• pp.73-80
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• 2004

Two red pepper(Capsicum annuum L.) cultivars, 'Geumtap' and 'Bugang', were cultivated at main producing areas in Korea and harvested four times in 1998 and 1999. Length, weight, dry matter ratio and color of red peppers were measured at each harvest. The relations between external characteristics with regional climatic elements such as total accumulated air temperature, total amount of precipitation and duration of sunshine during the fruit growing periods were compared. Length and fruit weight decreased by delayed harvest. These characteristics were shown significant difference according to cultivars, but not to growing year. Dry matter ratio had a tendency to increase at the 3rd and 4th harvest. The red color as measured a${\times}$L values of pepper powder, increased with being later in harvesting, but the trend was uncertain. In fruit growth, fruit length showed a positive correlation with total accumulated air temperature in later growth period of fruits(from 1st to 20th day before harvesting), and fruit weight had relationships with accumulated air temperature and total amount of precipitation in later growth period of fruit. There was no relation between dry matter ratio and climate elements. The red color was shown high correlations with total amount of precipitation and duration of sunshine. There was highest coefficient of determination($r^2$) in all external characteristics.

• Microbiology and Biotechnology Letters
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• v.4 no.3
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• pp.111-115
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• 1976

A strain of Enterobacter cloacae isolated from soil showed the evidence of growth in medium containing 1,500 ppm of Cd$\^$＋＋/ though its growth was inhibited at high cadmium concentrations. This strain accumulated 59％ of cadmium into the cells during incubation in medium containing 0.5 ppm of cadmium and its uptake was nearly proportional to the dry weight of cells, the average being 7.8 mg cadmium per g dry cells. It was also found that 60-70％ of cadmium accumulated in cells were distributed in the cell wall fraction.

• Journal of Drive and Control
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• v.11 no.4
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• pp.39-45
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• 2014

With recent oil price increases, the fuel efficiency of hydraulic excavators has become a serious issue. Researchers have considered weight lightening by high pressurization in order to improve the efficiency of the excavator and pump controlled actuation (PCA) and to reduce pressure loss of hybrid and valves using mechanical inertia. However, high pressurization is not very effective because the excavator operates at a low speed; a hybrid is inefficient because little accumulated inertial energy is accumulated; and PCA is ineffective because control precision and responsibility are low. In this study, a method to minimize air and gas in hydraulic oil has been presented as a simple and new way to increase hydraulic efficiency.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.3
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• pp.405-414
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• 2008

The purpose of this study was to understand the relationship between a chef's physical pain complaints and occupational circumstances. Cause-and-effect analysis of employees whose work was related to cooking was analyzed in relation to, - factor of cooking circumstances (kitchen condition, cooking operation, cooking allocation and distance of movement, cooking facilities) in order to ascertain possible links to physical pain. Cooking operations appeared to causes pain, especially in neck, shoulders, and legs. Cooking allocation and distance of movement, affected backache, eye-tiredness, and change of body-weight. Cooking space and facilities was related to, pain in waist, neck, shoulders, legs, arms, wrists, and back. Because cooking operations require extended concentrations and intensive effort, inappropriate occupational circumstances can lead to employees who usually suffer from accumulated.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.239-243
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• 1990

In this study the authors investigated the distribution of cadmium accumulated in cadmium-iun tolerant Hansenula anomala B-7 cells and also the effect of cadmium on protein synthesis. 84.9% of the cadmium accumulated was distributed in the soluble fraction (cytosol, etc.). The intracellular protein content was decreased by cadmium (1,000 $\mu g$/ml), but the content of soluble protein preeipitated by ammonium sulfate (30-75% saturation) was increased compared with the content of it obtained from the cells grown without cadmium. Furthermore, in the cells grown with 1,000 $\mu g$/ml of cadmium t h higher molecular weight soluble protein was increased compared with the cells grown without caa, mium, but the lower molecular weight soluble protein was decreased. These results suggested that the protein synthesis was inhibited by cadmium, but synthesis of higher molecular weight soluble protein was remarkably stimulated by cadmium.

• Journal of Environmental Health Sciences
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• v.22 no.1
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• pp.51-56
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• 1996

In order to detect the T-2 toxin accumulation in the animal tissues, T-2 toxin, produced by Fusarium sporotrichioides M-1-1, was injected to mouse by 0, 1 and 2 mg per kilogram of body weight, respectively, and T-2 toxin extracted from serum and organs were analyzed by the indirected competitive ELISA. The indirect competitive ELISA established in the laboratory can be check less than 0.1 ppb level of T-2 toxin and average recovery of T-2 toxin spiked was 80~113% in animal samples such as serum, liver and kidney. After 6 weeks of treatment with 2 mg of T-2 toxin per kg body weight, T-2 toxin was accumulated in serum (133.0 ng/ml), liver(1.4 ng/g) and kidney(14.3 ng/g) of mouse injected with 2 mg of toxin per kg body weight.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.5
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• pp.129-137
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• 2002

Elastomers, which are used engine mounts and body mount rubbers, are traditionally designed for NVH use in vehicles, and for vibration isolation in specific frequency range. According to the measurement of the characteristics of the SUV's engine mounts, there are variability in same engine mount properties. Static and dynamic stiffness of the SUV's engine mounts are changed due to the driving miles accumulated. The pre-load of body mount rubbers are changed due to the empty vehicle weight, passenger's weight and gross vehicle weight. And the dynamic stiffness of body mount rubbers are changed very hard above 150Hz frequency range.