• Environmental Engineering Research
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• v.20 no.2
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• pp.141-148
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• 2015

The presence of phenols in treated palm oil mill effluent (POME) is an environmental concern due to their phytotoxicity and antimicrobial activity. In this study, phenol-degrading bacteria, Methylobacterium sp. NP3 and Acinetobacter sp. PK1 were immobilized on oil palm empty fruit bunches (EFBs) for removal of phenols in the treated POME. The bacterial exopolysaccharides (EPS) were responsible for cell adhesion to the EFBs during the immobilization process. These immobilized bacteria could effectively remove up to 5,000 mg/L phenol in a carbon free mineral medium (CFMM) with a greater degradation efficiency and rate than that with suspended bacteria. To increase the efficiency of the immobilized bacteria, three approaches, namely activation, acclimation, and combined activation and acclimation were applied. The most convenient and efficient strategy was found when the immobilized bacteria were activated in a CFMM containing phenol for 24 h before biotreatment of the treated POME. These activated immobilized bacteria were able to remove about 63.4% of 33 mg/L phenols in the treated POME, while non-activated and/or acclimated immobilized bacteria could degrade only 35.0%. The activated immobilized bacteria could be effectively reused for at least ten application cycles and stored for 4 weeks at $4^{\circ}C$ with the similar activities. In addition, the utilization of the abundant EFBs gives value-added to the palm oil mill wastes and is environmentally friendly thus making it is attractive for practical application.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.241-244
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• 1999

The effect of Milliwrap and Uniwrap attached to the lid on the growth and acclimation of lily growing in vitro was investigated. Milliwrap treatment increased the plant height 1.6 and 1.2 times higher than control in 'Dame Blanche' and '94-36', respectively, plant height of 'Dame Blanche' was 6.1㎝ and '94-36' line was 13.4 ㎝. Two treatments slightly inhibited the fresh weight in 'Dame Blanche', whereas Milliwrap and Uniwrap enhenced the fresh weight more than 30% in '94-36'. Leaf width (1.0 ㎝) of Milliwrap treatment was most wide, which was 2.5 times higher than that of control. In the fresh weight of bulblets, the fresh weight per bulblet of treatment was higher than control in '94-36', but the number of bulblets per scale were few in the treatment to control. In the air composition of culture vessel, ethylene content (0.03 ppm) was low in the Milliwrap treatment compared with the control. CO₂ content of control was higher than treatments as 0.11% and it was about 3 times to air condition of out side. The acclimation ratio of Milliwrap treatment was higher as 85.1% and control was similar with Uniwrap treatment.

• Korean Journal of Environmental Agriculture
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• v.36 no.2
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• pp.106-112
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• 2017

BACKGROUND: Kale (Brassica oleracea) biosynthesizes various phytochemicals including glucosinolates, flavonoids, and carotenoids. Phytochemicals of plants are influenced by light, temperature, carbon dioxide, and growing conditions. Specifically, carotenoids are affected by temperature, light, and oxygen. The aim of this study was to investigate the effect of cold stress (day/night: $25^{\circ}C/20^{\circ}C$, $20^{\circ}C/15^{\circ}C$, $15^{\circ}C/10^{\circ}C$) on carotenoids in kale leaves. METHODS AND RESULTS: Kale was grown in pots for up to 50 days after sowing (DAS) in a greenhouse. For cold acclimation experiments, kale grown in growth chambers for 3 days and was subjected to low temperature for 4 days. The conditions maintained in the growth chambers were as follows: photoperiod, 12/12 h (day/night); light, fluorescent; and relative humidity, 60%. Carotenoid (lutein, ${\alpha}-carotene$, zeaxanthin, ${\beta}-carotene$) contents were analyzed by high-performance liquid chromatography (HPLC). The total carotenoid content gradually increased during cold acclimation for 3 days. When kale was subjected to cold stress, the total carotenoid content was high at $25^{\circ}C/20^{\circ}C$ treatment, but low at $15^{\circ}C/10^{\circ}C$ treatment. The total carotenoid content of kale leaves continuously grown in greenhouse decreased from 50 to 57 DAS (1,418 and 1,160 mgkg-1 dry wt., respectively). The lutein, ${\alpha}-carotene$, and ${\beta}-carotene$ contents were very low and the zeaxanthin contents were very high at $15^{\circ}C/10^{\circ}C$ treatment. When kale was subjected to cold stress, the ratio of individual to the total carotenoid contents of kale leaves was 4553% for -carotene and 210% for zeaxanthin. CONCLUSION: The ${\beta}-carotene$ and zeaxanthin contents in kale leaves indicate their sensitiveness toward cold stress.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.394-402
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• 2009

Adventitious shoots were induced from pinnae, petiole and rhizome in Pteris cretica 'Wilsonii' in order to develop the efficient mass propagation method, using in vitro culture. Only homogenized rhizome segments could regenerate young sporophytes. Efficient regeneration of multiple shoots was obtained on the one-eighth strength MS medium containing 1% sucrose, and $50mg{\cdot}L^{-1}$ $NaH_2PO_4$. To achieve higher rate of regeneration from rhizome segments, rhizome segments were exposed to growth regulators for 2 months and then subcultured on hormone-free medium. The greatest shoot regeneration was obtained by $1{\mu}M$ kinetin with $5{\mu}M$ NAA. BA was effective in formation of GGB (kind of meristems), but they showed low shoot regeneration rate. Plants obtained from present experiments were transplanted to examine good environmental conditions for acclimation. Juvenile plants obtained by the one-eighth strength MS medium showed highest survival rate and vigorous growth at the seedling stage.

• Environmental Engineering Research
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• v.11 no.3
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• pp.156-163
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• 2006

In this study, substrate removal characteristics were analyzed to reduce the cost of external carbon dosage at Sudokwon Landfill Site Management Corporation in Korea by utilizing oxygen uptake rate (OUR) and nitrate uptake rate (NUR) tests. To estimate and evaluate the substrate removal characteristics obtained by the batch tests, the lab-scale MLE process was operated. By-products of J Co. (sugar manufactory) and S Co. (fine chemical industry) were selected as the concerned carbon sources through a comparison of carbon and nitrogen contents. MeOH was tested as a control experiment. Until the steady state, the fraction of $RBDCOD_{OUR}$ concentration to COD concentration of J Co., S Co. by-products and MeOH increased and reached levels of 98%, 82%, and 100%, respectively. During the 20th operating day, the fraction of $RBDCOD_{NUR}$ concentration to COD concentration was 95%, 81%, and 83%, respectively. These fractions of $RBDCOD_{NUR}$ concentration to $RBDCOD_{OUR}$ concentration increased according to acclimation periods and reached levels of 99%, 97%, and 81%, respectively, on the 20th day. The results obtained from the lab-scale MLE process operation using the concerned carbon sources as external carbon were similar to that observed by OUR and NUR tests.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.37-71
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• 2021

Rapeseed is a typical winter crop, and its freezing stress tolerance is a major feature for winter survival. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of rapeseed under freezing stress conditions. This study investigates the physical and transcriptome changes of two rapeseed lines, 'J8634-B-30' and 'EMS26', under cold acclimation and freezing temperature treatments. The proline content of 'J8634-B-30' at 5 ℃ increased 8.7-fold compared to that before treatment, and there was no significant change in that of 'EMS26' RNA-sequencing analysis revealed 5,083 differentially expressed genes (DEGs) of 'J8634-B-30' under cold acclimation condition. Among the genes, 2,784 (54.8%) were up-regulated and 2,299 (45.2%) were down-regulated. The DEGs of 'EMS26' under cold acclimation condition were 5,831 genes, and contained 2,199 up-regulated genes (37.7%) and 3,632 down-regulated genes (62.3%). Among them, only DEGs annotated in the cold response-related signaling pathways were selected, and their expression in the two rapeseed lines was compared. Comparative DEGs analysis indicated that cold response related signaling pathways are proline metabolism and ABA (Abscisic acid) signaling. And ICE (Inducer of CBF expression) - CBF (C-repeat-binding factor) - COR (Cold-regulated) signaling were the significantly differentially expressed transcripts in the two rapeseed lines. The major induced transcripts of 'J8634-B-30' induced P5CS (Δ'-pyrroline-5-carboxylate synthetase), which is related to proline biosynthesis, PYL (pyrabactin resistance-like protein, ABA receptor) and COR413 (cold-regulated 413 plasma membrane 1). In conclusion, these result provide a foundation for understanding the mechanisms of freezing stress tolerance in rapeseeds. Further functional studies should be performed on the freezing stress-related genes identified in this study, which can contribute to the transgenic and molecular breeding for freezing stress tolerance in rapeseed.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.E2
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• pp.74-81
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• 2007

Feasibility test for a biofilter was performed to treat VOCs. The applied loading rate to the biofilter was calculated between 60 to $3,700\;kg\;COD/m^3$. Trimethyl-pentene and trimethyl-hexene were the two most dominant compounds and they occupy about 85 percent. During the acclimation period, it is desirable for a biofilter to receive relatively lower VOCs concentration and flow rate, until it can adjust to new substrate and operational environment. Temperature at various points inside the biofilter reactor was observed with more than 23 temperature sensors. With steam heating, temperatures of the top sections of the media were greater than those of bottom sections. Without steam heating, intermediate stages generally had higher temperature measurement than those of bottom and top stages. Because the pH values for different biofilter materials vary significantly, measurement of the pH for the mixture of different combinations of biofilter materials is necessary. Based on the types and brands of media, the measured pH ranged from 5.38 to 9.10. The range of measured pH of different mixtures with perlite, compost, saw dust, peat moss, limestone, vermiculite was 7.05 to 8.62.

• Animal cells and systems
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• v.14 no.1
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• pp.17-23
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• 2010

We isolated warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of olive flounder and investigated the mRNA expression of Wap65 and HSP70 in olive flounder exposed to osmotic (17.5, 8.75, and 4 psu) and thermal stress (25 and $30^{\circ}C$). The mRNA expression of Wap65 and HSP70 was increased by thermal stress. The mRNA expression of HSP70 was also increased by osmotic stress, whereas no significant change in Wap65 expression was detected. These results indicate that Wap65 mRNA expression occurs specifically in response to increases in water temperature, but not in response to osmotic stress. Plasma cortisol levels were also increased by osmotic and thermal stress. We also utilized the stress hormone cortisol to examine whether Wap65 expression is thermal-stress-specific. Cortisol treatment increased HSP70 mRNA expression in vitro, but had no significant effect on Wap65 mRNA expression. Thus, thermal stress, but not osmotic stress, induces Wap65 expression.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.1
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• pp.96-101
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• 1998

Substrate inhibition of 2,4-dinitrophenol (DNP) degradation was investigated using activated sludge which had been adapted to mineralize DNP. DNP is a metabolic uncoupler, preventing cells from making energy for growth and it has been suggested that pH may be important in mitigating effects of uncouplers. After acclimation of the activated sludge, the effect of pH on toxicity of DNP at high concentration (75 mg/L) was investigated, over a pH range of 5 to 9. DNP inhibition was found to be strongly dependent on mixed liquor pH. The DNP degradation rate was highest in the pH range of 6.95 to 7.84; at pH 5.94 degradation of 75 mg/L DNP was significantly inhibited; at pH < 5.77, DNP degradation was completely inhibited after approximately 30% of the DNP was degraded. By comparison, no significant effect of pH variation in the same range was seen on glucose uptake by the activated sludge culture.

• Journal of Plant Biology
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• v.26 no.1
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• pp.33-39
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• 1983

Twigs bearing floral buds of Rhododendron yedoense var. poukhanense(Ryp) and R. sinmsii(Rs), hardy species in Korea, were used to investigate the cold hardiness in relation to the developmental and anatomical characteristics, and the changes of water content. In floral buds of both species, the reproductive organs, pistils and stamens, matured within the bud scales in early ctober to prepare for a cold acclimation. The ray parenchymatous areas occupied in xylem were 41% in Ryp and 38% Rs. As the storage temperature is raised from 2 to 17$^{\circ}C$, water content increased more highly in Rs than in Ryp. Exotherm temperature of floral bud in Ryp was generally lower than that in Rs. The result supports that the higher cold-hardiness is achieved owing to the earlier maturation of floral buds, to the larger area of ray parenchyma in xylem, and to the lesser increase of water content as the storage temperature is raised.