• Advances in concrete construction
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• v.16 no.5
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• pp.255-267
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• 2023

The production of ultra-early-strength concrete (UESC) traditionally involves complexity or necessitates high-temperature curing conditions. However, this study aimed to achieve ultra-early-strength performance solely through room-temperature curing. Experimental results demonstrate that under room-temperature (28℃) curing conditions, the concrete attained compressive strengths of 20 MPa at 4 hours and 69.6 MPa at 24 hours. Additionally, it exhibited a flexural strength of 7.5 MPa after 24 hours. In contrast, conventional concrete typically reaches around 20.6 MPa (3,000 psi) after approximately 28 days, highlighting the rapid strength development of the UESC. This swift attainment of compressive strength represents a significant advancement for engineering purposes. Small amounts of steel fibers (0.5% and 1% by volume, respectively) were added to address potential concrete cracking due to early hydration heat and enhance mechanical properties. This allowed observation of the effects of different volume contents on ultra-early-strength fiber-reinforced concrete (UESFRC). Furthermore, the compressive strength of 0.5% and 1% UESFRC increased by 16.3% and 31.3%, respectively, while the flexural strength increased by 37.1% and 47.9%. Moreover, toughness increased by 58.2 and 69.7 times, respectively. These findings offer an effective solution for future emergency applications in public works.

• Advances in concrete construction
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• v.16 no.2
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• pp.119-126
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• 2023

Steel fiber reinforced self-compacting concrete (SFRSCC) has good workability such as high flowability and good cohesiveness. The workability, compressive strength, splitting tensile strength, and anti-penetration characteristics of three kinds of SFRSCC were investigated in this paper. The fraction of steel fibers of the SFRSCC is 0.5%, 1.5% and 2.0% respectively. The results of the static tests show that the splitting tensile strength increases with the increase of fraction of steel fibers, while the compressive strength of 1.5% SFRSCC is lowest. It is demonstrated that the anti-penetration ability of 1.5% SFRSCC subjected to a velocity projectile (200-500 m/s) is better than 0.5% and 2.0% SFRSCC according to the experimental results. Considering the steel fiber effects, the existing formula is revised to predict penetration depth, and it is revealed that the revised predicted depth of penetration is in good agreement with the experimental results. The conclusion of this paper is helpful to the experimental investigations and engineering application.

• Advances in concrete construction
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• v.16 no.5
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• pp.269-276
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• 2023

In order to study the effect of rubber particle size and admixture on the frost resistance of self-compacting concrete, three self-compacting concrete specimens with equal volume replacement of fine aggregate by rubber particles of different particle sizes were prepared, while conventional self-compacting concrete was made as a comparison specimen. The degradation law of rubber aggregate self-compacted concrete under freeze-thaw cycles was investigated by fast-freezing method test. The results show that the rubber aggregate has some influence on the mechanical properties and freeze-thaw durability of the self-compacting concrete. With the increase of rubber aggregate, the compressive strength of self-compacting concrete gradually decreases, and the smaller the rubber aggregate particle size is, the smaller the effect on the compressive strength of the matrix; rubber aggregate can improve the frost resistance of self-compacting concrete, and the smaller the rubber particle size is, the more obvious the effect on the improvement of the frost resistance of the matrix under the same dosage. Through the research of this paper, it is recommended to use 60~80 purpose rubber aggregate and the substitution rate of 10% is chosen as the best effect.

• Advances in concrete construction
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• v.16 no.3
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• pp.141-153
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• 2023

In order to develop and take full advantage of pasture fiber and waste concrete, this article studied how different amounts of pasture fiber influenced the toughness and pore structure of concrete with different replacement rates of recycled fine aggregate. Pasture fiber recycled concrete constitutive equations were established under idealized stiffness and toughness damage rate, based on fracture energy and damage mechanics theories. The relationship between pore structure and toughness was studied utilizing nuclear magnetic resonance and fractal theory. The toughness of text groups (0% (JZ), 10% (ZS10), 20% (ZS20)) first increased and then decreased with increasing amounts of pasture fiber, based on the damage rate of toughness. The toughness of concrete samples with recycled fine aggregate and pasture fiber is negatively correlated to the fractal dimension of small and medium-sized pores with a pore size of 0-500 nm. At a replacement rate of 10% of the recycled fine aggregate, the fractal dimension of the air voids (r: 500-9000 nm, i.e., Lg(r) ∈ [2.7, 3.9]) shows a gradual decrease with the increase of grass fiber dosage, indicating that with such a replacement rate of the recycled fine aggregate, the increase of pasture fiber can reduce the complexity of the pore structure of the air voids (500-9000 nm).

• Advances in concrete construction
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• v.16 no.6
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• pp.317-325
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• 2023

Strength prediction and correlation of concrete is done using experimental and analytical methods. Main objective is to correlate the experimental and simulated values of compressive strength of concrete mix using Fly Ash (FA) and Silica Fume (SF) by partial replacement of cement in concrete. Mix proportion was determined using IS method for M40grade concrete. Hundred and forty-seven cubes were cast and tested using Universal Testing Machine (UTM). Genetic Algorithm (GA) model was developed using C++ program to simulate the compressive strength of concrete for various proportions of FA and SF replacements individually at 3% increments. Experiments reveal that 12 percent silica fume replacement produced maximum compressive strength of 35.5 N/mm², 44.5 N/mm² and 54.8 N/mm² moreover 9 percent fly ash replacement produced a maximum strength of 31.9 N/mm², 37.6 N/mm² and 51.8 N/mm² during individual material replacement of concrete mix. Correlation coefficient for each curing period of fly ash and silica fume replaced mix were acquired using trend lines. The correlation coefficient is found to be approximately 0.9 in FA and SF replaced mix irrespective of the mix proportion and age of concrete. A higher and positive correlation was found between the experimental and simulated values irrespective of the curing period in all the replacements.

• Advances in concrete construction
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• v.16 no.4
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• pp.217-230
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• 2023

Methods for designing the post-tensioned anchorage zones at ultimate limit state has been specified in current design codes based on strut-and-tie models (STM). However, it is still not clear how to estimate the serviceability behavior of the anchorage zones. The serviceability is just indirectly taken into account by means of the reasonable reinforcement detailing. To address this issue, this paper is devoted to developing a modified strut-and-tie model (MSTM) to predict the behavior of concentric anchorage zones throughout the loading process. The principle of stationary complementary energy is introduced into STM at each load step to satisfy the compatibility condition and generate the unique MSTM. The structural behavior of anchorage zones can be achieved based on MSTM from loading to failure. Simplified formulas have been proposed to estimate the first cracking load, bearing capacity and maximum crack width with the consideration of the details of reinforcement bursting bars. The proposed model provides a definite method to control the bursting crack width in concentric anchorage zones. Four specimens with different bearing plate ratios have been designed and tested to validate the proposed method.

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1082-1091
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• 2024

The antioxidant capacity and protective effect of peptides from protein hydrolysate of Cordyceps militaris cultivated with tussah pupa (ECPs) on H₂O₂-injured HepG2 cells were studied. Results indicated ECP1 (<3 kDa) presented the strongest antioxidant activity compared with other molecular weight peptides. Pretreated with ECPs observably enhanced survival rates and reduced apoptosis rates of HepG2 cells. ECPs treatment decreased the ROS level, MDA content and increased CAT and GSH-Px activities of HepG2 cells. Besides, the morphologies of natural peptides from C. militaris cultivated with tussah pupa (NCP1) and ECP1 were observed by scanning electron microscopy (SEM). Characterization results suggested the structure of NCP1 was changed by enzymatic hydrolysis treatment. Most of hydrophobic and acidic amino acids contents (ACC) in ECP1 were also observably improved by enzymatic hydrolysis. In conclusion, low molecular weight peptides had potential value in the development of cosmetics and health food.

• Tuberculosis and Respiratory Diseases
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• v.44 no.3
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• pp.534-546
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• 1997

Background : The p53 and retinoblastoma(Rb) tumor suppressor genes are associated with the pathogenesis of several types of human cancer. Substantial proportion of the primary lung cancers or cell lines have been reported to have the p53 and/or the Rb gene mutations. But, so far there is no report on the analysis of the Rb gene polymorphism as one of the genetic susceptibility marker. This study was undertaken to establish the gene frequencies of the polymorphic genotypes of the p53 and Rb genes in Koreans to evaluate the possible involvement of these genotypes as a risk factor of lung cancer. Methods : In this study 145 controls without previous and present tumor history and 128 lung cancer patients were subjected to analysis. The two intragenic polymorphisms of the p53 gene(exon 4/ AccII, intron 6/MspI) and one intron 17/XbaI polymorphism of the Rb gene were analysed by the method of polymersae chain reaction- restriction fragment length polymorphisms(PCR-RFLPs). The genotype of the intron 3/16 bp repeat polymorphism of p53 was determined by PCR and direct gel electrophoresis. Results : There were no significant differences in the genotype distributions of the p53 gene between lung cancer patients and controls. But heterozygotes(Arg/Pro) of the exon 4/AccII polymorphisms were slightly over-represented than controls, especially in the Kreyberg type I cancer, which was known to be associated with smoking. The intron 3/16 bp duplication and the intron 6/MspI polymorphisms were in complete linkage disequilibrium. About 95% of the individuals were homozygotes of the common alleles both in the 16 duplication and MspI polymorphisms, and no differences were deteced in the genotype distributions between lung cancer patients and controls. Overall genotype distributions of the Rb gene polymorphisms between lung cancer patients and controls were not significantly different However, the genotype distributions in the Kreyberg type I cancer were significantly different from those of controls(p = 0.0297) or adenocarcinomas(p = 0.0008). It was noticeable that 73.4% of the patients with adenocarcinomas were heterozygotes(r1/r2) whereas 39.2% of the Kreyberg type I cancer were heterozygous at this polymorphisms. In the lung cancer patients, significant differences were also noted between the high dose smokers and low dose smokers including non-smokers(p = 0.0258). The relative risk to Kreyberg type I cancer was significantly reduced in the individuals with the genotype of r1/r2(odds ratio = 0.46, 95% C.I. = 0.25-0.86, p = 0.0124). The combined genotype distribution of the exon 4 AccII of the p53 and the intron 17 Rb gene polymorphisms in Kreyberg type I cancers were significantly different from dose of controls or adenocarcinomas. The highest odds ratio were observed in the individuals with the genotypes of Arg/Pro and r2/r2(odds ratio = 1.97,95% C.I. = 0.84-4.59) and lowest one was in the patients with Arg/Arg, r1/r2 genotype(odds ratio = 0.54, 95% C.I. = 0.25-1.14). Conclusion : The p53 and the Rb gene polymorphisms modulate the risk of smoking induced lung cancer development in Koeans. However, the exact mechanism of risk modulation by these polymorphism remains to be determined. For more discrete clarification of associations between specific genotypes and lung cancer risk, the evaluations of these polymorphisms in other ethnics and more number of patients will be needed.

• Advances in concrete construction
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• v.2 no.1
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• pp.13-27
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• 2014

The principal objective of this study is to deepen the characterization studies already led on sand concretes in previous works. Indeed, it consists in studying the effect of the sand type on the main properties of sand concrete: fracture and mechanical properties. We particularly insist on the determination of the fracture characteristics of this material which apparently have not been studied. To carry out this study, four different types of sand have been used: dune sand (DS), river sand (RS), crushed sand (CS) and river-dune sand (RDS). These sands differ in mineralogical nature, grain shape, angularity, particle size, proportion of fine elements, etc. The obtained results show that the particle size distribution of sand has marked its influence in all the studied properties of sand concrete since the sand having the highest diameter and the best particle size distribution has given the best fracture and mechanical properties. The grain shape, the angularity and the nature of sand have also marked their influence: thanks to its angularity and its limestone nature, crushed sand yielded good results compared to river and dune sands which are characterized by rounded shape and siliceous nature. Finally, it should further be noted that the sand concrete presents values of fracture and mechanical properties slightly lower than those of ordinary concrete. Compared to mortar, although the mechanical strength is lower, the fracture parameters are almost comparable. In all cases, the sand grains are debonded from the paste cement during the fracture which means that the crack goes through the paste-aggregate interface.

• The Korean Journal of Mycology
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• v.23 no.3 s.74
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• pp.202-208
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• 1995

This study describes the effects of several components on PCR amplification used for RAPD. We used different concentrations of reaction components to obtaine discrete and reproducible PCR products from Pleurotus cornucopiae. The optimum concentrations of reaction components were found to be 80 ng of template DNA, 30 pmole of 10-mer primer, $200\;{\mu}M$ dNTP, 2mM $MgCl_2$, 50 mM KCl, 10 mM Tris-HCl(pH 9.0), 0.1% Triton X-100, 1.5 unit of Taq DNA polymerase (promega) in $50\;{\mu}l$ reaction volume. The optimum annealing temperature was $35^{\circ}C$. These results proved to be valuable for characterization of Pleurotus spp.