• 생명과학회지
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• 제19권6호
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• pp.756-764
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• 2009

Scutellariae baicalensis GEORGt Acorus gramineus SOLAND and Gastrodia elata BLUME are traditional medicines used in the treatment of incipient stoke. In this study we investigated their effects on various aspects of neuronal differentiation in single or composite forms. Water-extracts of these medicines showed neuroprotective effects on cultured rat cortical neurons in normoxia and hypoxia. To understand the mechanism for neuroprotection we carried out various cell biological assays. They stimulated initial differentiation of neuronal development (transition from stage 1 to 2), and increased the number of spines and the length and number of dendritic processes. These effects were best manifested in the experimental group, which were given a mixture of the three kinds of extracts (p<0.01). To assess improvement of brain functions we carried out Morris water-maze tests for the mice that were fed on these extracts instead of water for 4 weeks. The experimental groups, especially those which were given the mixture of the three kinds of extract, showed significant (p<0.01) enhancement in memory as early as one day after the learning trial. These results indicate that these three kinds of extracts have synergistic effects on neuronal protection and improvement of brain functions.

• 한국자원식물학회지
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• 제21권5호
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• pp.347-351
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• 2008

뿌리에 진통, 진정, 건위, 구충 효능이 있다고 알려진 석창포(Acorus gramineus Soland)의 확대재배를 위하여 현재 분근증식만으로 한정되어 있는 번식법을 개선하고자 종자번식과 기내배양에 의한 번식 방법을 검토하였다. 종자번식을 위한 수확적기는 종피가 연녹색을 띠었을 때가 적합하였으며, 종자의 보관은 $5^{\circ}C$에서 30일까지 저장하는 것이 발아율을 높게 유지할 수 있었다. 석창포의 기내발아에는 $2.0mg{\cdot}L^{-1}$ NAA와 $0.1mg{\cdot}L^{-1}$ BA를 MS 기본배지에 첨가하여 다수의 신초를 유도한 후, $0.1mg{\cdot}L^{-1}$ BA 단독 처리 및 $0.5mg{\cdot}L^{-1}$ NAA와 $1.0mg{\cdot}L^{-1}$ BA 혼합처리에서 발근을 유도하는 것이 효과적이었다. 석창포 육묘상토로는 vermiculite보다 원예상토가 생육이 우수하였다.