• 한국토양동물학회지
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• 제2권2호
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• pp.76-82
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• 1997

Mortality of some agro-forest insect pests, Agrotis segetum, Blitopertha orientalis, Agerastica coerulea, Glyphodes perspectalis and Acantholyda parki caused by entomopathogenic nematodes was investigated in laboratory and in pot. Steinernema carpocapsae All and Pocheon strain were effective against 2nd of 3rd instar larvae of A. segetum showing 100% mortalities at the concentration of 10Ijs/larva but not effective against 4th inster larvae. Mortality of B. orientalis was 100% after 26 days in H. bacteriopora NC 1, H. bacteriophora Hamyang and S. glageri NC strain treatment. S. monticulum also showed 100% mortalities against A. coerulea andd G. perspectalis at the concentration of 80 and 40 Ijs/larva. However, H. bacteriophora Cheju and S. glaseri Cheju strain were not effective against A. parki, i.e., showing 23.3 and 20.0% mortalities, respectively at the concentration of 160Ijs/larva. S. glaseri Hanrim strain was more effective than H. bacteriophora Cheju strain against Pryeria sinica.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.131-136
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• 2005

Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a pr1-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the pr1-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, pr1 genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111 bp including 52 bp intron. The deduced Pr1-like peptide showed a low identity with Pr1s of entomopathogenic fungi such as B. bassiana Pr1 (BbPr1) and M. anisopliae Pr1 (MaPr1) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity $(>65\%)$ with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Pr1-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.

• 한국농림기상학회지
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• 제16권1호
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• pp.29-38
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• 2014

임분 밀도가 410tree $ha^{-1}$이며 평균 흉고직경이 $29.1{\pm}5.2cm$인 경기도 태화산의 V영급 잣나무 조림지에서 흉고직경을 독립변수로 하고 부위별 바이오매스를 종속변수로 하는 상대생장식을 만들었다. 잣나무 넓적 잎벌 피해로 인해 잎의 바이오매스가 낮게 나타났으나, 총 바이오매스에 미치는 영향은 미미하였다. 그간의 연구를 종합한 결과, 개체목 지상부 상대생장식(logY=a+blogX)의 모수 a는 평균 흉고직경과 임분밀도에 가장 큰 영향을 받는 것으로 나타났으나 b는 영향을 미치는 인자를 찾을 수 없었다. 임분 단위에서는 부위별로 잎은 6.68Mg $ha^{-1}$, 가지는 18.82Mg $ha^{-1}$, 줄기는 101.02Mg $ha^{-1}$로 나타났으며 이를 합한 지상부 총 바이오매스는 126.53Mg $ha^{-1}$로 나타났다. 국내 잣나무 임분의 지상부 바이오매스는 임령보다는 평균 흉고직경과 임분 밀도에 더 큰 영향을 받는 것으로 나타났으며 두 인자를 사용하여 국내 잣나무 임분 바이오매스를 98% 이상 설명하는 간단한 회귀식을 도출할 수 있었다.