• Proceedings of the Korean Society of Life Science Conference
• /
• 2000.06a
• /
• pp.11-14
• /
• 2000

• Journal of Plant Biotechnology
• /
• v.37 no.2
• /
• pp.125-132
• /
• 2010

Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.

• Journal of Plant Biotechnology
• /
• v.35 no.4
• /
• pp.307-316
• /
• 2008

Transposon-mediated insertional mutagenesis is one of powerful strategy for assessing functions of genes in higher plants. In this report, we have selected highly susceptible and tolerance plant by screening about high salt (3% NaCl) and cold stresses ($4^{\circ}C$) from F2 seeds of 30,000 Ac/Ds insertional mutagenesis lines in rice (Oryza sativa L. cv. Dongjin). In order to identify the gene tagging, insertion of Ds element was analyzed by Southern blot and these results revealed that 19 lines were matched genotype of selected lines with phenotype from the first selected 212 lines, and 13 lines have one copy of Ds elements. The Franking Sequence Tags (FSTs) of selected mutant lines showed high similarities with the following known function genes: signal transduction and regulation of gene expression (transpoter, protease family protein and apical meristem family protein), osmotic stress response (heat shock protein, O-methyltransferase, glyceraldehyde-3-phosphate dehydrogenase and drought stress induce protein), vesicle trafficking (SYP 5 family protein) and senescence associated protein. The expression pattern of 19 genes were analyzed using RT-PCR under the abiotic stresses of 9 class; 250mM NaCl, osmotic, drought, 3% $H_2O_2$, $100{\mu}M$ ABA, $100{\mu}M$ IAA, 0.1 ppm 2,4-D, $4^{\circ}C$ cold and $38^{\circ}C$ high temperature. Isolated knock-out genes showed the positive response about 250 mM NaCl, drought, $H_2O_2$, PEG, IAA, 2,4-D, ABA treatment and low ($4^{\circ}C$) and high temperature ($38^{\circ}C$). The results from this study indicate that function of selected knock-out genes could be useful in improving of tolerance to abiotic stresses as an important transcriptional activators in rice.

• Journal of Plant Biotechnology
• /
• v.35 no.3
• /
• pp.177-183
• /
• 2008

Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.

• Proceedings of the KIEE Conference
• /
• 1992.07b
• /
• pp.1211-1214
• /
• 1992

AC servo motor drives, Fara DS series, proposed in this paper can be effectively used in robots, CNC machine tools, and FA system with AC servo motors as actuators. The inverter of the AC servo drive consists of IGBT (Insulated Gate Bipolar Transistor) which have high switching frequency. Noises and vibrations generated in variable speed control of AC servo motors can be greatly reduced due to their high switching frequencies. In the developed servo drive, maximum torque is always generated in the whole speed range by compensating phase shift, which results from the nonlinearies of the AC servo motor during abrupt acceleration and deceleration. Abundant protection functions are provided to prevent abnormal state of the servo motor, and furthermore diverse user options are considered provided for the effective application. The proposed AC servo motor drive is designed to minimize velocity variation with respect to external load, supply voltage, environmental temperature, and humidity, so can be widely used in the fields of factory automation including robots and CNC msachine tools.

• Journal of Power Electronics
• /
• v.12 no.1
• /
• pp.88-97
• /
• 2012

This paper presents a method for the digital control of a high power factor AC/DC converter employing the power balance control technique to achieve a fast response of the output voltage control. To avoid the effects of an output voltage ripple in the voltage control loop, the average output voltage is sampled and used as a feedback signal for the output voltage controller. The proposed control technique was verified by simulations using MATLAB/Simulink and its implementation was realized by a dsPIC30F4011 digital signal processor to control a CUK topology AC/DC converter with a 48V output voltage and a 250 W output power. The experimental results agree with the simulation results. The proposed control technique achieves a fast transient response with a lower line current distortion than is achieved when using a conventional proportional-integral controller and the power balance control technique with the conventional sampling method.

• Journal of Power Electronics
• /
• v.8 no.2
• /
• pp.131-140
• /
• 2008

This paper presents a new control approach of DSTATCOM (distribution static compensator) for compensation of reactive power, unbalanced loading and harmonic currents under unbalanced non-sinusoidal ac mains. The control of DSTATCOM is achieved using Adaline based current estimator based on LMS algorithm to maintain source currents real and undistorted. The dc bus voltage of voltage source converter (VSC) working as DSTATCOM is maintained at constant voltage using a proportional-integral (PI) controller. The DSTATCOM system alongwith proposed control scheme is modeled in MATLAB to simulate the behavior of the system. The practical implementation of the DSTATCOM is carried out using dSPACE DS1104 R&D controller having TMS320F240 as a slave DSP. Simulated and implementation results are presented to demonstrate the effectiveness of the DSTATCOM with Adaline based control to meet the severe load perturbations with different types of loads (linear and non-linear) under distorted and unbalanced AC mains.

• Proceedings of the Korean Society of Life Science Conference
• /
• 2001.02a
• /
• pp.85-85
• /
• 2001

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2020.06a
• /
• pp.138-138
• /
• 2020

• Proceedings of the Botanical Society of Korea Conference
• /
• 1999.07a
• /
• pp.11-15
• /
• 1999

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1,700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.