• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.4
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• pp.427-434
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• 2014

Although ex situ target strength (TS) measurements using dual- and split-beam systems have become the primary approach of estimating fish abundance, theoretical model estimation is a powerful tool for verifying the measurements, as well as for providing values when making direct measurements is difficult. TS values for 20 samples of live bambooleaf wrasse (Pseudolabrus japonicus) whose target length (TL) ranged between 13.7 and 21.3 cm were estimated theoretically using the Kirchhoff-ray mode model, and the TS values for 18 live fish samples were additionally measured at ${\sim}0^{\circ}$ tilt angle to the swimming aspect using a tethered method at a frequency of 120 kHz to verify the theoretical values. The digitizing intervals used to extract the fish body and swim bladder morphology in the X-ray photographs significantly affected the calculated TS patterns, but variations based on the speed of sound and density ratio values for the general range of fish flesh were relatively small (within 1 dB). Close agreement was observed between the measured and theoretical TS values, and the correlation between the average TS and body length of the fish could be calculated accurately as <$TS_{120kHz}$>= 20logTL (cm) -71.6 using the theoretical method.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.380-385
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• 2010

Several theoretical acoustic scattering models were applied to estimate the target strength (TS) for assessing the biomass of zooplankton, to overcome the difficulty of direct measurements. Acoustical scattering characteristics of copepods were estimated using three theoretical models, and the application of the models was evaluated for four frequencies of a scientific echo sounder. The scattering directivity by the body shapes of copepods at 200 kHz and 420 kHz was significantly affected by TS patterns. Averaged TS, however, were similar at higher frequencies. Consequently, a low frequency model, such as a truncated fluid sphere model, provides a good acoustical biomass estimation. The regressions of TS and 30 logL were < $TS_{200\;kHz}$ >= 30logL-118.4 ($R^2=0.716$) and < $TS_{420 kHz}$ > =30 logL-108.8 ($R^2=0.758$), respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.360-372
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• 2020

Objective: Specific genomic sites can be recognized and permanently modified by genome editing. The discovery of endonucleases has advanced genome editing in pigs, attenuating xenograft rejection and cross-species disease transmission. However, off-target mutagenesis caused by these nucleases is a major barrier to putative clinical applications. Furthermore, off-target mutagenesis by genome editing has not yet been addressed in pigs. Methods: Here, we generated genetically inheritable α-1,3-galactosyltransferase (GGTA1) knockout Yucatan miniature pigs by combining transcription activator-like effector nuclease (TALEN) and nuclear transfer. For precise estimation of genomic mutations induced by TALEN in GGTA1 knockout pigs, we obtained the whole-genome sequence of the donor cells for use as an internal control genome. Results: In-depth whole-genome sequencing analysis demonstrated that TALEN-mediated GGTA1 knockout pigs had a comparable mutation rate to homologous recombination-treated pigs and wild-type strain controls. RNA sequencing analysis associated with genomic mutations revealed that TALEN-induced off-target mutations had no discernable effect on RNA transcript abundance. Conclusion: Therefore, TALEN appears to be a precise and safe tool for generating genomeedited pigs, and the TALEN-mediated GGTA1 knockout Yucatan miniature pigs produced in this study can serve as a safe and effective organ and tissue resource for clinical applications.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.305-312
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• 2018

This study was carried out to investigate euphausiid abundance in an acoustic survey from March 2010 to July 2010 to suggest reasonable usage and management of the euphausiid Euphausia pacifica in the coastal area of Guryongpo Pohang. The acoustic data were analyzed by the 2-frequency difference method and the distorted wave born approximation acoustical theoretical model, which is used for organisms of weak target strength with small scatter, such as euphausiid scatter, among other marine organisms. The distribution and monthly density of euphausiids were estimated in the survey area. The results show that the volume back scattering strength frequency difference for euphausiid was 13.91-7.6 dB, and their monthly averag density was $28.2g/m^2$.

• Korean Journal of Ecology and Environment
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• v.44 no.4
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• pp.337-346
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• 2011

We investigated nutrient loading and trophic states in a coastal estuarine system in the Asan estuary by assessing phytoplankton biomass and using the trophic index (TRIX). The monthly and yearly nutrient loading (TN, TP) from freshwater discharge from the Asan and Sapgyo reservoirs into the estuary were estimated and analyzed with related factors. Monitoring data (physio-chemical and biological variables) collected at five estuary stations were used to assess trophic states. Descriptive statistics of total phytoplankton cells, chl a concentrations and primary productivity were also used to assess seasonal trophic status. N loading from freshwater ranged $1.0{\sim}1.3{\times}10^4$ ton yearly. The yearly P loading ranged between 350 and 400 ton during 2004~2006, increasing to 570 ton in 2007. Regression results suggest that DIN and DSi were correlated with freshwater discharge at the upper region. Based on phytoplankton biomass and total cell abundance, the trophic state of the estuary was found to be eutrophic during spring due to phytoplankton bloom. Primary productivity level was remarkably high, especially in summer coinciding with high nutrient loading. Pheopigments increased during warm seasons, i.e. summer and fall. Trophic index results indicate that the trophic state varied between mesotrophic and eutrophic in the estuary water body, especially in the upper region. The results suggest that phytoplankton production was regulated by nutrient loading from freshwater whereas biomass was affected by other properties than nutrient loading in the Asan Estuary ecosystem.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.19 no.2
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• pp.93-98
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• 1983

When detecting underwater targets using echo sounder, the echo signals varies with the angular displacement of the transducer due to ship's motion. Then, the effect of the angular which effects the capability of the echo sounder must be investigated in relation to the detection of fish concentration, particularly, the abundance estimation of fish. In this pater, as the basic research to investigate the effect of the ship's motion when estimating the fish stocks by acoustic methods, the authors was theoritically considered the variations in the values of the two way directivity function of transducer in the direction of the targets which caused by the angular motion of transducer. Here, the effects of the movement of target and ship's running are neglected. At the same time, the data was applied to estimate the angular shift of the detectable area of echo sounder due to transducer displacement. From the results analyzed, we found that the angular shift of the detectable area due to ship's motion increases both as the roll angle increases and as the beamwidth becomes narrower, varies with the depth.

• Animal cells and systems
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• v.14 no.4
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• pp.291-296
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• 2010

Population ecological characteristics of growth and mortality play an important role in understanding the population dynamics of marine mammals. The instantaneous coefficients of natural and bycatch mortality were estimated for minke whales (Balaenoptera acutorostrata) in Korean waters using a population assessment model composed of bycatch and abundance data. The survivorship curve of this population was fitted to the data, and then the curve was revised using age-specific relative bycatchability coefficients ($q_t$). Instantaneous coefficients of natural and bycatch mortality of minke whales were estimated as 0.024/year and 0.076/year, respectively, and from this the survival rate was estimated as 0.905. This estimated survival rate was comparable to other cetaceans in other regions. The $q_t$ for this population ranged from 0.020 to 0.193. The revised survival rates were higher when the $q_t$ was taken into account. The mortality coefficient, survival rate, $q_t$ and survivorship curves had not previously been determined for minke whale in this area. This estimate could serve as fundamental information to assess the status of this population and for conservation and rational management.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.3
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• pp.202-209
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• 2000

In order to estimate demersal fishes using acoustic echo sounders and echo integrators, we consider several problems that are accurate bottom detection, optimum bottom offset and dead zone. The dead zone where no fish detection are summed distance resolution by the half pulse length of transmitted pulse and beam angle above the seabed. This paper has considered the dead-zone correction method to be technically correct for survey of demersal fishes. A comparison between near-bottom SV profiles acquired in Funka Bay, Hokkaido, of Japan, the East China Sea and the Yellow Sea, of Korea, with before and after the bottom correction, shows that the SV obtained with after the bottom correction is 2∼3dB higher than before the bottom correction in Funka Bay, and 17dB higher in East China Sea, too.

• Journal of Ecology and Environment
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• v.39 no.1
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• pp.43-49
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• 2016

Fishways are constructed to provide longitudinal connectivity of streams or rivers where their flow has been altered by in-stream structures such as dams or weirs. Nature-like fishways have an additional function of providing fish habitats. In the study, we estimated the role of a nature-like fishway (length: 700 m, slope: 1/100) for fish habitat by using two dominant species in the Sangju Weir, Nakdong River, to calculate the optimal ecological flow rate using Physical HABitat SIMulation (PHABSIM). To identify the dominant species that used the fishway, we conducted trap monitoring from August to November 2012 at the fishway exit. The dominant species were Zacco platypus and Opsariichthys uncirostric amurensis with a relative abundance of 62.1% and 35.9%, respectively. Optimal habitat suitability indices (HSIs) for Z. platypus and O. u. amurensis were calculated as 0.6-0.8 m/s (water velocity) and 0.2-0.4 m (water depth), and 0.5-0.7 m/s (water velocity) and 0.1-0.3 m (water depth), respectively. The optimal ecological flow rates (OEFs) for Z. platypus and O. u. amurensis were 1.6 and 1.7 cubic meter per second (CMS), respectively. The results of the study can be used in a management plan to increase the habitat function of nature-like fishways in the Sangju Weir. This methodology can be utilized as an appropriate tool that can determine the habitat function of all nature-like fishways.

• Journal of The Korean Astronomical Society
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• v.48 no.1
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• pp.21-55
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• 2015

If the Universe is dominated by cold dark matter and dark energy as in the currently popular ${\Lambda}CDM$ cosmology, it is expected that large scale structures form gradually, with galaxy clusters of mass $M{\geq}10^{14}M_{\odot}$ appearing at around 6 Gyrs after the Big Bang (z ~ 1). Here, we report the discovery of 59 massive structures of galaxies with masses greater than a few times $10^{13}M_{\odot}$ at redshifts between z = 0.6 and 4.5 in the Great Observatories Origins Deep Survey fields. The massive structures are identified by running top-hat filters on the two dimensional spatial distribution of magnitude-limited samples of galaxies using a combination of spectroscopic and photometric redshifts. We analyze the Millennium simulation data in a similar way to the analysis of the observational data in order to test the ${\Lambda}CDM$ cosmology. We find that there are too many massive structures (M > $7{\times}10^{13}M_{\odot}$) observed at z > 2 in comparison with the simulation predictions by a factor of a few, giving a probability of < 1/2500 of the observed data being consistent with the simulation. Our result suggests that massive structures have emerged early, but the reason for the discrepancy with the simulation is unclear. It could be due to the limitation of the simulation such as the lack of key, unrecognized ingredients (strong non-Gaussianity or other baryonic physics), or simply a difficulty in the halo mass estimation from observation, or a fundamental problem of the ${\Lambda}CDM$ cosmology. On the other hand, the over-abundance of massive structures at high redshifts does not favor heavy neutrino mass of ~ 0.3 eV or larger, as heavy neutrinos make the discrepancy between the observation and the simulation more pronounced by a factor of 3 or more.