• Tuberculosis and Respiratory Diseases
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• 제44권4호
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• pp.914-921
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• 1997

저자들은 최근 가족력은 없지만 폐동정맥루와 간동맥혈관 조영술 및 위점막에서 관찰된 혈관기형을 동반한 Osler-Rendu-Weber 증후군 1예에서 폐동정맥루에 대해 금속코일을 이용한 색전요법을 시행하여 특별한 합병증 없이 증상의 개선을 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.295-300
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• 2012

Manganese ($Mn^{2+}$) is a trace element that is essential for normal physiology, and is predominantly obtained from food. Several lines of evidence, however, demonstrated that overexposure to $MnCl_2$ exerts serious neurotoxicity, immunotoxicity and developmental toxicity, particularly in male. The present study aimed to evaluate the effect of 0, 1.0, 3.3, and 10 mg/kg/day doses of $MnCl_2$ on the reproductive organs in the immature female rats. Rats (PND 22; S.D. strain) were exposed to $MnCl_2$ ($MnCl_2{\cdot}4H_2O$) dissolved in drinking water for 2 weeks. The animals were sacrificed on PND 35, then the tissues were immediately removed and weighed. Histological studies were performed using the uteri tissue samples. Serum LH and FSH levels were measured with the specific ELISA kits. Body weights of the experimental group animals were not significantly different from those of control group animals. However, ovarian tissue weights in 1 mg and 3.3 mg $MnCl_2$ dose groups were significantly lower than those of control animals (p<0.05 and p<0.01, respectively). Uterine tissue weights of 3.3 mg dose $MnCl_2$ groups were significantly lower than those of control animals (p<0.01), while the 1 mg $MnCl_2$ dose and 10 mg $MnCl_2$ dose failed to induce any change in uterine weight. Similarly, only 3.3 mg $MnCl_2$ dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05). Non-reproductive tissues such as adrenal and kidney failed to respond to all doses of $MnCl_2$ exposure. The uterine histology revealed that the $MnCl_2$ exposure could affect the myometrial cell proliferation particularly in 3.3 mg dose and 10mg dose group. Serum FSH levels were significantly decreased in 1mg $MnCl_2$ dose and 10 $MnCl_2$ mg groups (p<0.05 and p<0.01, respectively). In contrast, treatment with 1 mg $MnCl_2$ dose induced a significant increment of serum LH level (p<0.05). The present study demonstrated that $MnCl_2$ exposure is capable of inducing abnormal development of reproductive tissues, at least to some extent, and altered gonadotropin secretions in immature female rats. Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the $Mn^{2+}$ might be a potential environmental mediator which is involved in the female pubertal process.

• 한국수정란이식학회지
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• 제26권1호
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• pp.79-84
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• 2011

This study was performed to identify the differentially methylated region (DMR) and to examine the mRNA expression of the imprinted H19 gene in day 35 of SCNT pig fetuses. The fetus and placenta at day 35 of gestation fetuses after natural mating (Control) or of cloned pig by somatic cell nuclear transfer (SCNT) were isolated from a uterus. To investigate the mRNA expression and methylation patterns of H19 gene, tissues from fetal liver and placenta including endometrial and extraembryonic tissues were collected. The mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. Bisulfite analyses demonstrated that the differentially methylated region (DMR) was located between -1694 bp to -1338 bp upstream from translation start site of the H19 gene. H19 DMR (-1694 bp to -1338 bp) exhibits a normal mono allelic methylation pattern, and heavily methylated in sperm, but not in oocyte. In contrast to these finding, the analysis of the endometrium and/or extraembryonic tissues from SCNT embryos revealed a complex methylation pattern. The DNA methylation status of DMR Region In porcine H19 gene upstream was hypo methylated in SCNT tissues but hypermethylated in control tissues. Furthermore, the mRNA expression of H19 gene in liver, endometrium, and extraembryonic tissues was significantly higher in SCNT than those of control (p<0.05). These results suggest that the aberrant mRNA expression and the abnormal methylation pattern of imprinted H19 gene might be closely related to the inadequate fetal development of a cloned fetus, contributing to the low efficiency of genomic reprogramming.

• Toxicological Research
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• 제23권1호
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• pp.79-86
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• 2007

The isolated plantamajoside from Plantago asiatica that is often used as a marker compound in chemotaxonomic studies has various bioactivites such as the inhibitions of cyclic AMP phosphodi-esterase and 5-lipoxygenase, microbial growth and inflammation, and currently demands the generation of toxicity data. The purpose of this study was to examine the toxicities of the single and 14 days repeated dose toxicity in Sprague-Dawley rats orally administrated with plantamajoside at dose levels of 0, 500, 1000, and 2000 mg of dried material/kg body weight/day. The results showed that there was no difference in body weight change, food intake, water consumption, or relative organ weight among different dose groups. Also we observed no death and abnormal clinical signs were observed during the experimental period. Between the groups orally administered Plantago asiatica and the control group, there was no statistical significance in hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by 14 days treatment with Plantago asiatica. These results suggest that no observed adverse effect level (NOAEL) of the oral application was considered to be more than 2000 mg/kg in rats under the conditions employed in this study. Another observation was performed to investigate the safety of Plantago asiatica in respect of genotoxicity. This substance was examined that Salmonella typhimurium reversion assay (Ames test) in strain TA98, TA100, TA1535. In the reverse mutation test, Plantago asiatica did not induce mutagenicity in Samonella typhimurium with and without metabolic activation. These results indicated that Plantago asiatica had no genotoxicity.

• 멤브레인
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• 제28권1호
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• pp.55-61
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• 2018

급진적인 산업발전에 따른 무분별한 이산화탄소 배출로 인하여 지구온난화, 이상 기후 등의 문제가 불거지고 있으며 이는 한 나라에만 국한되지 않은 국제적인 문제가 되었다. 위와 같은 문제를 해결하고자 본 연구에서는 이산화탄소에 대해 높은 친화도를 가지고 있는 PEGBEM-g-POEM 가지형 공중합체를 합성하였으며 $CaCO_3$염을 첨가하여 염의 중량에 따른 혼합매질분리막의 이산화탄소/질소 투과 성능을 관찰하였다. $CaCO_3$염이 고분자 중량 대비 50% 첨가되었을 때 최적의 이산화탄소/질소 분리 투과성능을 보였으며, 선택도는 염을 추가하기 전 44.7에서 45.42로 크게 변하지 않은 반면 이산화탄소 투과도는 염을 첨가하기 전 22.5 GPU에서 28.16 GPU로 약 25% 향상되었다. 이는 첨가한 염과 이산화탄소간의 상호작용으로 인하여 향상된 고분자 복합막의 이산화탄소에 대한 용해도에 기인하는 것으로 생각된다. 또한 염을 과량으로 첨가한 경우에는 고분자가 복합막의 표면을 충분히 메우지 못하여 계면결함이 생성되었으며 이로 인해 이산화탄소 기체 분리성능이 감소하였다. 기체분리성능 향상을 위해서는 적정량의 염 첨가가 필수적인 것으로 분석되었다.

• 한국해안·해양공학회논문집
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• 제23권1호
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• pp.34-42
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• 2011

지구온난화로 인한 태풍 강도 증가와 장기해수면 상승에 대처하기 위하여 호안 및 방파제의 마루높이를 고정식으로 증가시키는 것이 일반적이다. 그러나 이와 같은 대처방식은 월파저지에는 효과적이나 수변공간의 바다 조망권을 저하시키는 단점을 가지고 있다. 본 연구에서는 황천 시에 전개시키고 평상 시 격납하여 월파저지와 조망권 확보 기능을 동시에 달성할 수 있는 일종의 동적 가변형 구조물인 고무막체 파라펫의 개발을 시도하였다. 본 구조물은 순전히 공기의 주입 및 배출에 의하여 제어되는 무인 작동 구조물로서 해황에 따라 원격제어가 가능하다. 고무막체 파라펫의 여러 최적형상이 도출되었으며 비선형 유한요소 프로그램을 사용한 수치해석 및 수리모형실험을 통하여 이들 파라펫들의 전개 및 격납 과정, 그리고 월파발생시의 거동 및 구조적 안정성을 검토하였다.

• 항공우주정책ㆍ법학회지
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• 제25권2호
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• pp.201-230
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• 2010

자동차, 철도, 광산업이 시작되면서 등장한 새로운 위험에 대하여 사회적 유용성을 이유로 활성화된 세계를 유지하고자 출발한 허용된 법리에 대하여 우리나라의 학계에서는 다수가 인정하고 있는 분위기인데 반하여 판례에서는 아직까지 기존의 과실론 체계로 해석하고 있다. 그러나 첨단 과학기술영역의 하나인 항공 운항에 있어서 사건이나 사고의 원인이 되는 청천난류(CAT)나 요란(turbulence)을 갖고 있는 뇌우는 환자의 불안전한 예후처럼 실시간 완벽한 탐지가 곤란하고 위험 발현 여부와 크기를 확정할 수 없으며 수시로 변화하는 이상 기상 현상이다. 이러한 이유가 있음에도 청천난류(CAT)가 존재할 수 있는 jet 기류의 이용은 시간과 연료를 대폭 절감하고 치명적 위험으로 발현되지 않은 뇌우가 있는 공항으로의 접근 착륙은 승객들의 정시 출 도착을 보장하기에 사회적 유용성이 높은 운항 형태라고 할 수 있다. 위험이 예견되고 회피할 수 있는 방법이 있으나 사회적 유용성을 이유로 개연성이 낮은 청천난류(CAT)나 요란(turbulence) 위험 지역을 운항하다가 발생한 사건이나 사고에 대하여는 허용된 위험의 법리를 적용하여 조종사들의 일정한 주의의무에 대한 완화가 필요하다.

• Journal of Ginseng Research
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• 제14권2호
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• pp.291-296
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• 1990

Various rates of 2,4-D were sprayed on 2 and 3 year old ginseng plants as foliar spray to define the critical concentration. No apparent plant injury was noticable for those ginseng plants when application concentration of 2,4-D doubled the recommended dosage (70 ml/10a). Neither abnormal foliar change occurred nor any inhibition in leaf and stem growth was resulted for the plants treated with 2,4-D concentrated two times of the recommended dosage. When the rates of 2,4-D application were increased greater than this level, injury ratings increased linearly with the rates of 2,4-D application and plant you was inhibited. Ethylene gas was not produced from the ginseng plant treated with 2 times concentrated 2,4-D, however the ginseng plants produced 0.03 to 0.09 ppm ethylene gas when the rate of application were increased 3 and 4 times, respectively. On the other hand the soybean treated with the recommended amount of 21-D produced ethylene gas of 10-20 times higher compared with ginseng plants and died. Photosynthesis ability of the ginseng leaf was significantly decreased by 2,4-D foliar application but it was recovered 4 weeks after 2,4-D foliar treatment. The herbicide 2,4-D was applicated to 2,3 and 4 years old ginseng plants as foliar spray with the rates of 0.5, 1.0, 1.5 and 2.0 times of the recommended dosage to define the effects of 2,4-D on the plant growth and root yield of the ginseng. There were no significant differences in the leaf and stem growth between untreated and 2,4-D treated plant. Berry maturing of 3 and 4 year old ginseng was not influenced by 2,4-D. The root weight of 4 years old ginseng plant was not reduced by application of 2,4-D concenrated 2 times of the recommended dosage, Application time of the herbicide 2,4-D had no effects on the leaf or stem growth of 2,3 and 4 year old year old ginseng plants. When the ginseng seedling was treated with 2,4-D, detrimental phenomena as stem bending and docoration of seedling leaf margin occurred, but stem bending was recovered in a few day s. Keywords Panax ginseng C.A. Meyer, 2,4-D , herbicide.

• 한국농공학회지
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• 제34권4호
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• pp.59-68
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• 1992

A Study was made to improve soil and water temperature and mechanized farming for a wetted paddy under the influence of artesian groundwater located at Samcheon-Dong, Chuncheon City, Kangweon Province. Half perforated PVC drainage pipes were installed in the test paddy to observe temperature change of water and soil. The temperature of the water in the paddy and soil itself raised significantly after the installation of the half perforated PVC pipes. A subsequent improvement of growth and yield of rice on the paddy was achieved. Harvesting operation was also improved with firm ground condition so that cutting and threshing could be done simultaneously within the paddy plot. Following results were obtained from the study. 1.Temperature of the water in a paddy under the influence of artesian groundwater was not changed notably although air temperature was fluctuated during the crop period. Soil temperature was mostly affected by the artesian groundwater. However, the half perforated PVC pipe drainage system made it possible to raise temperature of water and soil remarkably up to the level of optimum farming. 2.Total precipitation was 534.Omm during the crop period of the paddy for 118 days from May 26 to September 20 in 1992. Due to heavy rainfalls of 105.6mm and 109.8mm occurred on August 7 and August 27.1992, respectively, the rate of the artesian groundwater increased to 35 litter per minute with two to three days of time lag. 3.Average rate of the artesian groundwater was 28 litter per minute from the one year of observation. The rate varied by 0.7 to 1.3 times of average during the observation period. Peak rate of the artesian groundwater decreased to 14.5 litter per minute when daily precipitation maintained at the amount of 20 to 30mm for a long time period. Contrarily, it showed a tendency to increase to 35 to 40 litter per minute when heavier precipitation of 50 to l00mm occurred in a short period of three to five days. 4.Growth and yield of Yemyung variety of rice planted on the paddy that was facilitated with a drainage system with half perforated PVC pipes were confirmed at a normal level, while paddy without this perporated drainage system showed abnormal growth with low yield.

• 유기물자원화
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• 제18권1호
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• pp.98-103
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• 2010

기후변화에 따른 집중호우와 장마로 댐 내에 가장 많이 유입되는 초목류와 2012년부터 해양투기가 전면 금지되는 하수슬러지의 자원재활용 방안으로 퇴비화 연구를 수행하였다. 처리구는 댐부유물 톱밥, 참나무수피, 하수슬러지, 계분을 30 : 20 : 40 : 10 (S-1)과, 댐부유물 톱밥, 참나무수피, 하수슬러지, 계분을 30 : 30 : 30 : 10(S-2)로 설정하여 퇴비화 과정 중 이화학적 특성 등을 분석하였다. S-1과 S-2 처리구 모두 퇴적더미 내부온도가 $65^{\circ}C$ 이상에서 10일 정도 유지되어 대부분의 병원균이 사멸되었고, 최종 90일째 pH는 S-1 처리구가 7.78로 S-2 처리구보다 약간 더 높았다. C/N율은 S-1 처리구가 15.3, S-2 처리구는 16.9 였다. 최종 제품의 품질은 부산물비료 퇴비공정규격에 적합하였다. 부숙도 평가에서 발아지수는 S-1이 91, S-2가 84로 S-1이 더 높은 부숙도를 보였다. 따라서 우수한 품질과 안전성, 그리고 댐부유물 톱밥과 하수슬러지의 이용량이 가장 많은 배합비율인 S-1(댐부유물 톱밥 : 참나무수피 : 하수슬러지 : 계분 = 30 : 20 : 40 : 10)으로 제품을 생산하는 것이 바람직하다.