• Title/Summary/Keyword: Abiotic factors.

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Splicing and alternative splicing in rice and humans

  • E, Zhiguo;Wang, Lei;Zhou, Jianhua
    • BMB Reports
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    • v.46 no.9
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    • pp.439-447
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    • 2013
  • Rice is a monocot gramineous crop, and one of the most important staple foods. Rice is considered a model species for most gramineous crops. Extensive research on rice has provided critical guidance for other crops, such as maize and wheat. In recent years, climate change and exacerbated soil degradation have resulted in a variety of abiotic stresses, such as greenhouse effects, lower temperatures, drought, floods, soil salinization and heavy metal pollution. As such, there is an extremely high demand for additional research, in order to address these negative factors. Studies have shown that the alternative splicing of many genes in rice is affected by stress conditions, suggesting that manipulation of the alternative splicing of specific genes may be an effective approach for rice to adapt to abiotic stress. With the advancement of microarrays, and more recently, next generation sequencing technology, several studies have shown that more than half of the genes in the rice genome undergo alternative splicing. This mini-review summarizes the latest progress in the research of splicing and alternative splicing in rice, compared to splicing in humans. Furthermore, we discuss how additional studies may change the landscape of investigation of rice functional genomics and genetically improved rice.

Identification of functional SNPs in genes and their effects on plant phenotypes

  • Huq, Md. Amdadul;Akter, Shahina;Nou, Ill Sup;Kim, Hoy Taek;Jung, Yu Jin;Kang, Kwon Kyoo
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.1-11
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    • 2016
  • Single nucleotide polymorphism (SNP) is an abundant form of genetic variation within individuals of species. DNA polymorphism can arise throughout the whole genome at different frequencies in different species. SNP may cause phenotypic diversity among individuals, such as individuals with different color of plants or fruits, fruit size, ripening, flowering time adaptation, quality of crops, grain yields, or tolerance to various abiotic and biotic factors. SNP may result in changes in amino acids in the exon of a gene (asynonymous). SNP can also be silent (present in coding region but synonymous). It may simply occur in the noncoding regions without having any effect. SNP may influence the promoter activity for gene expression and finally produce functional protein through transcription. Therefore, the identification of functional SNP in genes and analysis of their effects on phenotype may lead to better understanding of their impact on gene function for varietal improvement. In this mini-review, we focused on evidences revealing the role of functional SNPs in genes and their phenotypic effects for the purpose of crop improvements.

Molecular cloning and characterization of a soybean GmMBY184 induced by abiotic stresses

  • Chung, Eun-Sook;Kim, Koung-Mee;Lee, Jai-Heon
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.175-181
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    • 2012
  • Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184 (Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184 fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.

Long-term Environmental Changes and the Interpretations from a Marine Benthic Ecologist's Perspective (I) - Physical Environment

  • Yoo Jae-Won;Hong Jae-Sang;Lee Jae June
    • Fisheries and Aquatic Sciences
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    • v.2 no.2
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    • pp.199-209
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    • 1999
  • Before investigating the long-term variations in macrobenthic communities sampled in the Chokchon macrotidal flat in Inchon, Korea, from 1989 to 1996, we need to understand how environmental factors in the area vary. As potential governing agents of tidal flat communities, abiotic factors such as mean sea level, seawater, air temperature, and precipitation were considered. Data for these factors were collected at equal intervals from 1976 or 1980 to 1996, and were analyzed using a decomposition method. In this analysis, all the above variables showed strong seasonal nature, and yielded a significant trend and cyclical variation. Positive trends were seen in the seawater and air temperatures, and based upon this relationship, it was found that the biological sampling period of our program has been carried out during warmer periods in succession. This paper puts forth some hypotheses concerning the response of tidal flat macrobenthos communities to the changing environment including mild winters in succession.

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Environmental Factors and Bioremediation of Xenobiotics Using White Rot Fungi

  • Magan, Naresh;Fragoeiro, Silvia;Bastos, Catarina
    • Mycobiology
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    • v.38 no.4
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    • pp.238-248
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    • 2010
  • This review provides background information on the importance of bioremediation approaches. It describes the roles of fungi, specifically white rot fungi, and their extracellular enzymes, laccases, ligninases, and peroxidises, in the degradation of xenobiotic compounds such as single and mixtures of pesticides. We discuss the importance of abiotic factors such as water potential, temperature, and pH stress when considering an environmental screening approach, and examples are provided of the differential effect of white rot fungi on the degradation of single and mixtures of pesticides using fungi such as Trametes versicolor and Phanerochaete chrysosporium. We also explore the formulation and delivery of fungal bioremedial inoculants to terrestrial ecosystems as well as the use of spent mushroom compost as an approach. Future areas for research and potential exploitation of new techniques are also considered.

Abundance of Heterotrophic-and Photosynthetic Dinoflagellates and Factors Controlling Their Abundance and Distribution in Korean Coastal Waters During Summer, 1994

  • Lee, Won-Je;Park, Nam-Joo;Choi, Joong-Ki
    • Journal of the korean society of oceanography
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    • v.37 no.4
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    • pp.201-211
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    • 2002
  • We investigated the abundance and biomass of dinoflagellates and factors controlling their abundance in marine planktonic ecosystems in Korean coastal waters. The abundance of photosynthetic (PDNF) and heterotrophic dinoflagellates (HDNF) was in the range of 0.7${\times}$10$^2$ cells/1-14.0${\times}$10$^6$ cells/1 and in the range of 3.0${\times}$10$^2$ cells/1-6.47${\times}$10$^5$ cells/I, respectively. Their biomass was 0.5${\times}$10$^{-1}$-2.56${\times}$10$^4$ ${\mu}gC/I$ and 2.0${\times}$10$^{-1}$-1.5${\times}$10$^{2}$ ${\mu}gC/I$, respectively. In order to find factors controlling their abundance, stepwise regression and best subsets regression analyses were used. We found that during the summer the most important factors controlling PDNF abundance are DO, P, N and S (abiotic factors), and for HDNF, the abundance of zooplankton, ciliates and HF (biotic factors), and that high turbidity may effect the distribution of dinoflagellate species.

A Life Stage-based Model for Assessing the Walleye Pollock Gadus chalcogrammus Population in the East Sea (생활사 기반 모델을 이용한 동해 명태(Gadus chalcogrammus)의 개체군 평가)

  • Kim, Kyuhan;Sohn, Myoung Ho;Hyun, Saang-Yoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.1
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    • pp.65-76
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    • 2017
  • Since the late 1990s, walleye pollock Gadus chalcogrammus fisheries in Korean waters have been considered collapsed. Although many fisheries scientists suspect that the collapse might have been triggered by overexploitation of juvenile pollock or environmental changes, such conjectures have been neither tested nor investigated, partially because of limited data on the population. There has been no survey of the population, and the ages of fish in fishery catch have rarely been identified. Instead, fishery catch data from 1975-1997 included information about two life stages, 'juveniles and adults,' and data on catch-per-unit-effort (CPUE) during 1963-2007 and those on fish length and weight during 1965-2003 had been sporadically collected from commercial fisheries. To test hypotheses about the collapse of the pollock fisheries, we used a statistical linear model with juvenile CPUE as the response variable, and abiotic (e.g., water temperatures) and biotic factors [e.g., adult pollock, flatfishes (Pleuronectidae sp.), and sandfish (Arctoscopus japonicus) CPUEs] as the explanatory variables. The model results indicated that depletion of the pollock population was associated with both biotic (adult pollock and flatfishes abundance) and abiotic factors (mid-water temperatures in February and October). We further interpreted the results from ecological and biological perspectives, suggesting possible mechanisms.

Effect of Different Abiotic Factors on Chemotaxis of Bacteria Towards Fungal Propagules (종류가 다른 무생물적 요인이 진균류에 미치는 세균의 주화성)

  • Gupta, Sushma;Arora, Dilip K.;Pandey, Arun K.;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.20 no.2
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    • pp.109-117
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    • 1992
  • Chemotactic responses of five motile saprophytic and one phytopathogenic bacteria e.g. Agrobacterium radiobacter, Bacillus subtilis, B. polymyxa, Pseudomonas aeruginosa, P. fluorescens and Xanthomonas malvacearum towards exudate of Cochliobolus sativus conidia, Fusarium of oxysporum f. sp. ciceri chlamydospores, Macrophomina phaseolina sclerotia and Phytophthora drechsleri f. sp. cajani oospores were determined in vitro at different abiotic conditions. In general, a positive correlation (r=0.76 to 0.89; P=0.05) was observed between concentration of fungal exudates and attraction of bacterial cells. Similarly, a significant (P=0.05; r=+0.82 to 0.95) positive correlation was noticed between chemotactic response and incubation period. The chemotactic response of bacteria was greatly influenced by temperature and pH of the test fungal exudate. The optimum temperature for maximum chemotaxis was $25^{\circ}C$ for A. radiobacter, $30^{\circ}C$ for B. polymyxa, P. aerugionosa, P. fluorescens and X. malvacearum and $35^{\circ}C$ for B. subtilis. Fungal exudates maintained at pH 7 attracted maximum number of bacteria. The response of bacterial cells to exudates at pH 3 and 11 was not significantly (P=0.05) different than that to the buffer (control). Chemotaxis of bacteria was observed towards attractants (fungal propagules and their exudates) when they were kept apart and bridged with the capillaries filled with non-attractant (buffer) or attractant (exudate).

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Recovery Process of Forest Edge Formed by Clear-cutting Harvest in Korean Red Pine (Pinus densiflora) Forest in Gangwondo, South Korea (강원도 남부 지역에서 소나무림 벌채 후 형성된 숲 가장자리의 회복 과정)

  • Kim, Jun-Soo;Cho, Yong-Chan;Bae, Kwan-Ho
    • Journal of Korean Society of Forest Science
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    • v.106 no.1
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    • pp.1-9
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    • 2017
  • Forest harvest as large scale artificial disturbance makes edge environment in both clear-cutted and forested habitat. To clarify the development and recovery process of forest edge after disturbances is essential to understand vegetation responses and landscape level consequences such as edge-distance. In Korea, after clear-cutting, edge-related changes of environment and vegetation was not clarified yet. In Korean red pine (Pinus densiflora) forest, by applying space-for-time approach (sites with undisturbed and 1, 3, 10, 16 yr after cutting), the edge-related change of plant abundance and abiotic factors were determined for 20 line-transect (60 m) and 340 ($1m{\times}5m$) quadrats, and clarified depth and duration of the disturbance. Immediately after edge formation, within 15m form the edge, biotic and abiotic factors such as cover, richness, canopy openness, temperature and moisture content exhibited larger changes compared to forest interior. Plant abundance and abiotic variables were stabilized at the level of forest inside within 16 yr and 10 yr after edge creation, respectively. Woody (tree and shrub) species generally was showed larger increment with proximity to edge than did herb or graminoid species. In addition, negative interactions between woody and herbaceous species were detected during the period of forest edge closure. Our results suggested that depth of forest edge formed by clear-cutting of Korean red pine forest was at least from 15 m to 20 m and edge effect were likely sustained more than 16 years. As the first empirical study on edge-distance between two contrast habitats of clear-cutted and adjacent forest in South Korea, the analytical reality on landscape structure and habitat patches can be improved.