• Korean Journal of Veterinary Research
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• v.62 no.1
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• pp.5.1-5.5
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• 2022

Many countries have imposed regulations relating to concerns that hide contamination will affect the cleanliness of abattoirs. However, South Korea has not indicated any clear criteria. The purpose of this study is to use surrogate bacteria to measure the contamination in abattoirs caused by contaminated cattle hides. The swab contact method and plate count method are used. Surrogate bacteria are found in most internal environments after the final process. These surrogates remained on the carcass even after the final washing process. This paper is the first study in South Korea that use surrogate bacteria to analyze contamination levels in abattoirs.

• Korean Journal of Veterinary Service
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• v.44 no.1
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• pp.51-59
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• 2021

Various infectious diseases have threatened swine industries in Korea. One of the major risk factors of disease spread is livestock transport vehicle. For this reason, all abattoirs are required by law to have vehicle washing and disinfection facilities. However, detailed guidelines for cleaning and disinfection procedures were not provided. So in this study, assessment by visual observation was conducted to evaluate how well the cleaning and disinfection procedures of livestock transport vehicles have been performed in four different abattoirs in Jeonbuk, Korea. The observation included length of car washing time, the number of washing bay, type of washperson, type of disinfectant application, length of disinfection time, and compliance of driver's hygienic procedure. Every vehicle in all abattoirs conducted washing and disinfection procedure. However, there were procedural differences for each abattoirs and defects in the washing and disinfection procedures. In addition to that, the compliance of driver's hygienic procedure was not sufficient. Hence, detailed manuals on washing and disinfection procedures for involved people are required and it is necessary to establish an inspection and evaluation system.

• Korean Journal of Veterinary Research
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• v.60 no.3
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• pp.105-108
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• 2020

These days abattoirs' hygiene of Korea is regulated by Hazzard Analysis Critical Control Point (HACCP). Although 20 years have been left since first HACCP was adjusted in Korea, 12% of abattoirs got inconsistence on evaluations. Food poisoning caused by bacteria feces like pathogenic Escherichia coli and Salmonella has not decreased. These bacteria on meat cross-contaminate at the abattoir. Therefore, field verification of abattoir's critical control point (CCP) and experiments to find alternative ways of the CCP were conducted. The aerobic bacteria were measured before and after high-pressure water based washing process set as CCP in most abattoirs. Four parts of cattle carcasses were selected to apply sponge-swab method. The effects were < 1 log reduction which is not significant. Lactic acid (LC), chlorine dioxide (ClO₂) and slightly acidic electrolyzed water (SAEW) were used to measure the effect of reducing bacteria on beef by the different time. LC has 1.24-2.02 log reduction for aerobic bacteria. ClO₂ has 1.44-1.96 log reduction for aerobic bacteria. SAEW has 1.1-1.91 log reduction for aerobic bacteria. There was significant difference according to concentrations (p < 0.05). This study presents legitimacy for hygiene improvement of CCP by field verification. In addition, chemical disinfectants that can be mechanically applied have better reduction effects of high-pressure washing.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.390-402
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• 2023

Salmonella are Gram-negative pathogenic bacteria commonly found in food animals such as poultry and swine and potentially constitute risks and threats to food safety and public health through transmissible virulence and antimicrobial resistance (AMR) genes. Although there are previous studies in the Philippines regarding genotypic and phenotypic AMR in Salmonella, there are very few on virulence and their associations. Hence, this study collected 700 Salmonella isolates from swine samples in abattoirs and wet markets among four districts in Metro Manila and characterized their genotypic virulence and β-lactam AMR profiles. Gene frequency patterns and statistical associations between virulence and bla genes and comparisons based on location types (abattoirs and wet markets) and districts were also determined. High prevalence (>50%) of virulence genes was detected encompassing Salmonella pathogenicity islands (SPIs) 1-5 suggesting their pathogenic potential, but none possessed plasmid-borne virulence genes spvR and spvC. For bla, bla_TEM was detected with high prevalence (>45%) and revealed significant associations to four SPI genes, namely, avrA, hilA, mgtC, and spi4R, which suggest high resistance potential particularly to β-lactam antibiotics and relationships with pathogenicity that remain mechanistically unestablished until now. Lastly, comparisons of location types and districts showed variations in gene prevalence suggesting effects from environmental factors throughout the swine production chain. This study provides vital data on the genotypic virulence and AMR of Salmonella from swine in abattoirs and wet markets that suggest their pathogenicity and resistance potential for policymakers to implement enforced surveillance and regulations for the improvement of the Philippine swine industry.

• 축산식품과학과 산업
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• v.4 no.1
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• pp.7-19
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• 2015

• Journal of Animal Science and Technology
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• v.64 no.1
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• pp.155-165
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• 2022

As pork consumption increases, rapid and accurate determination of porcine carcass grades at abattoirs has become important. Non-destructive, automated inspection methods have improved slaughter efficiency in abattoirs. Furthermore, the development of a calibration equation suitable for non-destructive inspection of domestic pig breeds may lead to rapid determination of pig carcass and more objective pork grading judgement. In order to increase the efficiency of pig slaughter, the correct estimation of the automated-method that can accommodate the existing pig carcass judgement should be made. In this study, the previously developed calibration equation was verified to confirm whether the estimated traits accord with the actual measured traits of pig carcass. A total of 1,069,019 pigs, to which the developed calibration equation, was applied were used in the study and the optimal estimated regression equation for actual measured two traits (backfat thickness and hot carcass weight) was proposed using the estimated traits. The accuracy of backfat thickness and hot carcass weight traits in the estimated regression models through stepwise regression analysis was 0.840 (R²) and 0.980 (R²), respectively. By comparing the actually measured traits with the estimated traits, we proposed optimal estimated regression equation for the two measured traits, which we expect will be a cornerstone for the Korean porcine carcass grading system.

• Parasites, Hosts and Diseases
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• v.52 no.3
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• pp.331-334
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• 2014

Hydatid worms, hosted by humans and animals, impose serious human health risk and cause significant livestock production loss. To better understand the disease infection status in Xinjiang, China, we investigated the disease epidemics in 4 livestock animals, i.e., cattle, sheep (both sheep and goat), camels, and horses, slaughtered at the abattoirs in Urumqi, Yining, Tacheng, and Altay areas. The results showed that the animals were infected at different rates, in the order of sheep (9.8%), cattle (8.4%), camels (6.8%), and horses (4.3%). The infection rates were found to be different between the abattoirs in various regions even for the same animals. For sheep, the rates increased significantly as the animals grew older. It was 1.9% before 1 year of age and increased to 8.2% in the age of 1-2 years, and further increased to 12.3% when the animals were 3-4 years old, and reached 17.2% when they were 5-6 year old. Sheep older than 6 years had an infection rate of 19.5%. This study demonstrates that the 4 livestock animals in the pastoral areas in Xinjiang were infected by the parasites to various extend. This study is the first systematic investigation of the hydatid worms in various livestock animals in Xinjiang, China, which provides epidemiological information about the infection of hydatid worms in livestock, and is valuable in developing strategies for prevention and control of the hydatid disease.

• Korean Journal of Veterinary Research
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• v.7 no.2
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• pp.25-30
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• 1967

Pathological studies, on the lung of 27 cases of bovine parasitic bronchitis which were secured from abattoirs in Korea, were performed and discussed. The chief pathological findings were as follows. Grossly, the lungs were shown various degree of lobular consolidation, collapse and alveolar emphysema around the bronchi in which nematodes of Dictyocaulus viviparus were present. Emphysema and edema were observed in the interlobular septa. The lesions were encountered mostly in the caudal and ventral borders of the diaphragmatic lobes. In all the cased of parasitic bronchitis in which thickening of the visceral pleura and fibrinous deposition on the pleural surfaces were not seemed to be entirely due to the lungworm infection. Microscopically, bronchopneumonia, peribronchial and peribronchiolar lymphoid hyperplasia, lympho-reticular broncho-occlusive legion, and bleakthrough lesion were examined in the affected lungs.

• Korean Journal of Veterinary Service
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• v.16 no.2
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• pp.144-149
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• 1993

This study was conducted to investigate the infectious arthritis on pigs slaughter in eastern Kangweon. During the period of between Feb. and Nov. 1992, 459 pigs were sampled at six abattoirs. Bacterial isolation and identification were perfomed from the arthritic lesions. In addition, drug susceptibility for the major isolated microoganisms were examined. The results obtained were summarized as follows : 1. From arthritic lesions bacteria were most frequently isolated, isolation rate being 21.1%. 2. The bacteria isolated from arthritic lesion, Staphylococcus spp. (33.9%), Coli form(39.3%) were most frequently isolated, while the genera of Mycoplasma were detected less frequently. 3. Antimicrobial drug susceptibility of the major organisms showed that all the isolated bacteria were susceptible to SXT(91.0%), cephalothin(82.5%), but resisted to penicillin and streptomycin.

• Journal of Embryo Transfer
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• v.11 no.3
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• pp.241-248
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• 1996

This study was conducted to improve the production efficiency of in vitro produced (IVP) embryos in Korean Native cows. The optimal conditions and procedures for in vitro maturation(IVM), in vitro fertilization(IVF) and in vitro culture(IVC) of bovine follicular oocytes and IVP embryos were evaluated. Immature follicular oocytes were collected fiom the follicles of bovine ovaries obtained from abattoirs. The oocytes of Grade I and II for IVM were cocultured with monolayered bovine oviductal epithelial cells(BOEG) or granulosa cells in TCM-199 solution supplemented with follicle stimulating hormone, lutenizing hormone, estradiol-17$\beta$ and heat inactivated fetal calf serum at 39$^{\circ}C$ under 5% $CO_2$ in air for 14 to 24 hours. Most of the oocytes(93%) matured to metaphase II in 24 hours. The cocultured IVM oocytes were fertilized in vitro at significantly(P<0.05) higher rate with BOEC(83.8%) and with granulosa cells(84.6%) than the non-cocultured IVM oocytes(73.6%). The IVM-IVF embryos developed to morula and blastocyst at significantly(P<0.05) higher rate in coculture with BOEC(41.2%) than with granulosa cells(23.1%) or conditioned medium(23.4%).