• Korean Journal of Microbiology
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• v.38 no.2
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• pp.119-126
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• 2002

ATP concentrations far estimating total microbial biomass in the sediment were measured in three different marine sedimentary environments. ATP concentrations were highest in the surface sediment and decreased with increasing sediment depth and distance from the land. The results indicated that the benthic microbial biomass is primarily controlled by nutrient inputs from the overlying water column. Because of the longer residence time and adsorption to the sediment, the variations in organic carbon (OC) contents with sites and depths were not as distinct as that of ATP, and the correlation between OC and ATP was not significant in the coastal sediments. No significant correlation between OC and ATP in the coastal sediments also suggested that microbial biomass in the labile organic-enriched coastal sediment is suppressed by the grazing of higher trophic level such as meiofauna. Overall regional and vertical distribution of ATP indicated that h\`w can be a relevant tool for measuring total microbial biomass in various marine sedimentary environments.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.6
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• pp.817-824
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• 1997

Extracellular ATP elicits various biological responses and plays a significant role in physiological regulation. Recently, ATP-induced growth inhibitions were reported in some tumor cell lines, but these effects and mechanisms are not well hewn. This study was conducted to investigate ATP-induced growth inhibition in mouse $leukemic(P388D_1)$ cells. ATP inhibited cell growth in a dose-dependent manner as analyzed by MTS assay$(IC_{50}: 33.1\;{\mu}M)$. Nucleotides other than ATP, such as ADP$(37.5;{\mu}M)$ and AMP$(33.2;{\mu}M)$ had the same effects as ATP but adenosine$(57.8;{\mu}M)$ showed less effect than ATP. ATP attenuated the cells in $G_0/G_l\;and\;G_2/M$ phases but increased those in S phase in flow cytometric analysis. Hypodiploid cells$(A_0)$, the presumptive findings of apoptosis, were found among the ATP-treated cells. ATP induced DNA fragmentation into $180{\mu}200\;bps $as measured by electrophoresis. some apoptotic cells were stained by TUNEL method. ATP increased the intracellular free $Ca^{++}$concentration$([Ca^{++}]_i)$ and the increment of $([Ca^{++}]_i)$ was caused by influx from the extracellular space. These results suggest that extracellular ATP induces growth inhibition through apoptosis.

• Journal of Food Hygiene and Safety
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• v.15 no.3
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• pp.252-255
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• 2000

This study was conducted to investigate the application of ATP bioluminescence to measure the degree of microbial contamination from raw meat, meat processing and milk processing lines. Samples collected from slaughter house, meat and milk processing plants were tested for estimation of bacterial number by using ATP bioluminescence and conventional method. The former result was transffered to R-mATP value(log RLU/ml), and the latter transffered to CFU(log/ml). Correlation coefficient(r) between aerobic counts(CFU, log/ml) and R-mATP(log RLU/ml) value was 0.93(n=408). R-mATP of aerobic counts from beef, pork, chicken was 0.93(n=220), and that was 0.93(n=187) between meat processing and dairy processing plants. In addition, Correlation coefficient(r) between aerobic counts and R-mATP was 0.87(n=252) under 1$\times$10${^5}$/ml of bacterial count and 0.74(n=152) over 10${^5}$ respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.7
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• pp.2534-2540
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• 2010

In this paper we propose contents and results on installation and commissioning test for ATP on-board unit, which has propelled as one of the research projects, so called, "Reliability assessment and operation technology development for Korean-type tilting trains" in order to ensure the safety and operation efficiency of tilting trains. In KRRI, we have developed tilting trains for the speed-up of existing lines and for the passenger service improvement where the KTX is not available. And we also make progress the operation trial test at the speed of 10km with the use of ATS units, used in existing lines, for the purpose of the reliability assessment of the developed tilting trains. In MLTM, they have decided to accelerate the speed at more than 200km/h for the 7 existing lines including Jungang-line and Chungbuk-line where KTX has not operated. According to this decision, Train control system is to be changed from ATS to ATP. We should install an ATP on-board unit in tilting trains and verify the operational suitability. Therefore, we have installed and tested the same ATP on-board unit on tilting trains that used in the ATP construction project on Gyeongbu-line and Honam-line.

• Korean Journal of Food Science and Technology
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• v.31 no.5
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• pp.1345-1348
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• 1999

The utility of a bioluminescence adenosine triphosphate(ATP) assay method for estimating bacterial levels in mackerel(Scomber japonicus) was investigated. Mackerel was stored at $1^{\circ}C$ throughout 10 days and its RLU(relative light unit) and APC(aerobic plate count) was determined. The ATP bioluminescence assay was validated during the storage of 32 samples, resulting in an agreement between the ATP assay and standard plate count methods of over 90% credibility. Therefore, ATP bioluminescence assay was considered as a rapid and near real-time means in estimating the microbial load on mackerel skin.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.4
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• pp.419-425
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• 1998

To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ($[Ca^{2+}]_i$) mobilization and cell proliferation were investigated. ATP-induced $[Ca^{2+}]_i$ increased in a dose-dependent manner $(10^{-7}\;M{\sim}10^{-3}\;M)$. ATP-induced $[Ca^{2+}]_i$ increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced $[Ca^{2+}]_i$ increment was also inhibited by the pretreatment of U73122, a phospholipase C (PLC) inhibitor, in a time-dependent manner. Suramin, a putative $P_{2Y}$ receptor antagonist, dose-dependently weakened ATP-induced $[Ca^{2+}]_i$ mobilization. Significant increases in cell proliferation were observed at 2, 3, and 4 days after ATP was added. Stimulated cell proliferation was also observed with adenosine at days 2 and 3. This cell proliferation was significantly inhibited by the treatment with suramin. Ionomycin also stimulated cell proliferation in a concentration-dependent manner. In conclusion, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.

• Proceedings of the KSR Conference
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• 2009.05a
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• pp.476-482
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• 2009

A signal equipment improvement project for the existing facility ATS to ATP is under construction in Kyung-Bu and Ho-Nam commercial line including 400 rolling stocks. But there was a concern for the interface between KTX OBCS and ATP from the beginning of the project. At present, this is the front burner event. This paper introduces interface checking and predicted failure point between KTX OBCS and ATP improvement system. Through this measure the optimal installation of the ATP will be possible. The study was made under maintenance efficiency including reliability because the KTX signal equipment must qualify the above condition.

• Proceedings of the KSR Conference
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• 2008.11b
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• pp.1163-1168
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• 2008

Automatic Train Protection(ATP) System is developed by ETCS(European Train Control System) CONSORTIUM for unify Europe railway system. ATP system is composed of Eurobalise and EuroATC. Nowadays, Korea construct ATP system in conventional line and new electrical locomotives also install ATP system. It is important to verify quality which form checklist during Factory Acceptance Test at beyond the seas. In this paper, review the process, test item, norm and checklist for the Balise Transmission Module, Compact Antenna Unit, Vehicle Control Unit and Communication Controller at the Plymouth Bombardier and Vasteras enics factory.

• Archives of Pharmacal Research
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• v.16 no.1
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• pp.71-74
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• 1993

The protective effect of adenosine triphosphate (ATP) on gastic ulcer induced in rats has been studied. Gastic ulceration was induced by hypothemic restraint stress or dicolofenac sodium. Gastic acid secretion and mucosal injury produced by the hypothemic restraint stress was greater as compared with those produced by diclofenac sodifum. ATP significantly reduced area of injury, however, increased cyclic adenosine monophosphate (cATP) content. Administration of dipyridamole along with ATP did not change the total lesion area in both models when compared to ATP alone. Aminophyline antagonized antagonized the protective effect of ATP on the injured area. Famotidine was found to be effective in reducing gastric acid output as well as the total injured area without any change in cAMP content when given along with ATP.

• Journal of Ginseng Research
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• v.25 no.3
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• pp.127-129
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• 2001

Bioluminescence technique was applied to ginseng powders. ATP bioluminescence can be used as a rapid method that can be implemented for microbiological monitoring of contaminated ginseng powders. The RLU (relative light units) of ATP was proportion to bacterial CFU (colony forming units) when in high contaminated ginseng powders ($\geq$ about 1.0$\times$10$^4$CFU/g). However, when in low contaminated ginseng powders ($\times$10$^4$CFU/g), the RLU was not proportion to CFU, respectively.