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경안천 수질에 대한 환경기초시설 방류부하량의 영향 (The Effect of Discharge Loading at Sewage Treatment Plants on Water Quality in Kyoungan Stream)

  • 김호섭;이수웅;류덕희;공동수
    • 한국물환경학회지
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    • 제25권3호
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    • pp.452-458
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    • 2009
  • This study was carried out to assess the effect of discharge loading at sewage treatment plants (STP) on water quality in Kyoungan stream. The seasonal variations of water quality (BOD, COD, SS, TN, and chl.a) at site 1(Mesanribo) were similar with those at site 2(Sehabo). BOD, COD, SS, and chl.a concentration were also similar except for TN and TP concentration which were lower than those at site 1. The BOD, COD, TN, TP and chl.a concentration at site 2 in dry period were higher than that of wet period. The ratios of BOD, TP, COD, SS discharge loading at all STP in Kyoungan stream watershed to delivery loading at site 2 were 0.69, 0.54, 0.48, 0.31, and 0.26 on average, respectively. Both site 1 and site 2, the seasonal variations of discharge loading at STP did not showed any relationship with those of water quality. The mean ratios of inflow loading at site 1 to delivery loading at site 2 were relatively high that ranged from 0.43 to 0.61. In dry period, the ratios of discharge loading at STP in Sehabo watershed to delivery loading at site 2 were below 0.2 except for BOD (0.24), and decreased <0.1 in wet period. While, SS loading from watershed between site 1 and site 2 to delivery loading at site 2 ratios increased in wet period.

Genetic Screening for Plant Cell Death Suppressors and Their Functional Analysis in Plants

  • Yun, Dae-Jin
    • 한국생명과학회:학술대회논문집
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    • 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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    • pp.23-36
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    • 2005
  • Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces cell death when expressed In yeast. To investigate whether .Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various orgarusms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs In detail. PBIl is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorhodamine123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Bax-induced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower lovels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. H$_{2O2}$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of H2O2 treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased In the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MBP phosphorylation activity of AtMPK3 i'n vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation In situ. Thus, AtNDPK2 appears to play a novel regulatory role in H2O2-mediated MAPK signaling in plants.

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중력탐사(重力探査)에 의(依)한 경상층군내(慶尙層群內) 왜관(倭館)-포항간(浦項間)의 지하구조(地下構造) 연구(硏究) (Gravity Survey on the Subsurface Structure between Waekwan-Pohang in Kyoungsang Basin)

  • 민경덕;정종대
    • 자원환경지질
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    • 제18권4호
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    • pp.321-329
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    • 1985
  • The gravity measurement has been conducted at 113 stations with an interval of about 1km along the national road of about 120km running from Busangdong to Pohang through Waekwan, Daegu, Youngchun and Aankang. The subsurface geology and structure along the survey line is interpreted from Bouguer anomaly by applying Fourier method and Talwani method for two dimensional body. The mean depth of Moho discontinuity is 31.4km, and the depth decreases very slowly from inner continent toward east coast. The depth of Conrad discontinuity increases from 11km at the east coastal area to 17km at the inner continental area, and especially increases rapidly in the area between Waekwan to Busangdong. The depth of basement of Kyoungsang Basin inereases from near Waekwan toward Daegu upto about 4. 8km, and increases rapidly to reach the maximum depth of about 8.5km at 8km east of Daegu. But it starts to decrease from the place of 10km west of Youngchun, and is about 7.2km at Youngchun and about 6km at 6km east of Youngchun. The depth starts to increase smoothly beyond this point, and is 7km at 15km east of Youngchun. From this point, the depth starts to decrease again, and is about 3.8km at Ankang. The depth of basement of Pohang Basin is 500m at Pohang and about 650m at 5km west of Pohang. A massive granite body which is considered to be a part of Palgongsan Granite exposed at the depth of 1. 5km at 9km west of Youngchun. Another massive granite body is situated underneath the Pohang Basin at depth of 1.5 to 2km, and sedimentary rocks of Kyoungsang Group and volcanic rocks are distributed between Pohang Basin and this granite body. Finally, Yangsan Fault is identified at about 2.5km east of Ankang.

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Competitive Growth and Attachment of Listeria monocytogenes and Lactococcus lactis ssp. lactis ATCC 11454

  • Lee, Shin-Ho;Frank, Joseph-F.
    • Journal of Microbiology and Biotechnology
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    • 제2권2호
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    • pp.73-77
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    • 1992
  • The effect of a nisin-producing Lactococcus lactis spp. lactis (L. lactis) on the growth and attachment of Listeria monocytogenes Scott A and Brie 1 on stainless steel and their growth in Brain Heart Infusion broth was determined. Viable cells of Listeria decreased rapidly after 9~12 hr of incubation at $21^{\circ}C$ and after 6~9 hr of incubation at $32^{\circ}C$ in the presence of L. lactis. The number of L. monocytogenes Scott A attached to stainless steel in pure culture was $2.5{\times}10^3/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}2.3{\times}10^3/\textrm{cm}^2{\;}at{\;}32^{\circ}C$ after 48 hr of incubation, but was only $10/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}1.1{\times}10/\textrm{cm}^2{\;}at{\;}32^{\circ}C$ in the presence of L. lactis. Results from L. monocytogenes strain Brie 1 were similar to those from strain Scott A. The population of L. monocytogenes Scott A which attached to stainless steel with previously adherent L. lactis was $1.8{\times}10^2/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}8.2{\times}10^2/\textrm{cm}^2{\;}at{\;}32^{\circ}C$, whereas the population attached to sterile stainless steel was $1.2{\times}10^3/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}2.1{\times}10^2/\textrm{cm}^2{\;}at{\;}32^{\circ}C$. For L. monocytogenes Brie 1, the attached population of the control was $1.6{\times}10^4/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}3.2{\times}10^2/\textrm{cm}^2{\;}at{\;}32^{\circ}C$, and on stainless steel with adherent L. lactis, it was $1.1{\times}10/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}6.9{\times}10/\textrm{cm}^2{\;}at{\;}32^{\circ}C$. Surface adherent L. lactis was less inhibitory to attachment of L. monocytogenes on stainless steel than a liquid culture inoculum. Listeria attached to stainless steel survived dry storage for 20 days both in the presence and absence of adherent lactococci.

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함치성낭종과 단방성 법랑모세포종의 감별에 관한 방사선학적 연구 (A RADIOGRAPHIC STUDY OF DIFFERENTIAL DIAGNOSIS BETWEEN DENTIGEROUS CYSTS AND UNICYSTIC AMELOBLASTOMAS)

  • 나채영;최갑식
    • 치과방사선
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    • 제23권2호
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    • pp.255-264
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    • 1993
  • The purpose of this study was to obtain some informations for the radiographic differential diagnosis between dentigerous cysts and unicystic ameloblastomas in the mandible. The authors observed and compared the clinico-radiographic features of 38 cases of dentigerous cyst and 32 cases of unicystic ameloblastoma associated with impacted mandibular molar. The obtained results were as follows: Dentigerous cysts occurred the most frequently in the 3rd decade, but unicystic ameloblastomas in the 2nd decade, and both lesions occurred with slight predilection in males. Average of lesional size of unicystic ameloblastomas was larger than that of dentigerous cysts, and lesions of over 25㎠ were only in unicystic ameloblastomas. Cortical thinning and expansion were more frequently observed in unicystic ameloblastomas at 72.9% than in dentigerous cysts at 15.8%. Dentigerous cysts showed smooth border at 89.5%, but unicystic ameloblastomas showed smooth border at 53.1% and scalloped border at 46.9%. Dentigerous cysts showed well-defined outline at 81.6%, but unicystic ameloblastomas showed well-defined outline at 53.1% and moderate-defined outline at 46.9%. In both lesions, the mandibular 3rd molar was the most frequent causative tooth. Average of distance between the cemento-enamel junction and lesional wall attachment of the causative tooth was longer in unicystic ameloblastomas than in dentigerous cysts. Severe displacement of causative tooth was more frequent in unicystic ameloblastomas at 62.5% than in dentigerous cysts at 23.7%. Dentigerous cysts showed homogeneous lesional radiolucency at 89.5%, but unicystic ameloblastomas showed inhomogeneous lesional radiolucency at 53.1%. Root resorption of adjacent tooth and displacement of mandibular canal were more frequent in unicystic ameloblastomas at 65.2% and 61.5% than in dentigerous cysts at 15.8% and 38.1% respectively.

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Cone beam형 전산화 단층영상을 이용한 하악전치부 영양관의 연구 (Nutrient canals on mandibular anterior region in cone beam computed tomography)

  • 강정호;김규태;최용석;황의환
    • Imaging Science in Dentistry
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    • 제36권3호
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    • pp.137-143
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    • 2006
  • Purpose : To evaluate location, distribution, diameter, and length of the nutrient canals on mandibular anterior region using a cone beam computed tomography (CBCT). Materials and Methods : Mandibular CBCT was performed on 33 adults (18 males and 15 females) with no history of systemic disease, and any other dental surgery history Location, distribution, diameter, and length of the nutrient canals on mandibular anterior region were radiographically evaluated. A statistical comparison was done by SPSS. Results : In the location and distribution of nutrient canals, they were found in 6.8% at labial portion above root apex, in 93.28% at lingual portion above root apex in 46.2% at labial portion below root apex, and in 53.6% at lingual portion below root apex. Nutrient canals at lingual portion above root apex were most frequently observed between central and lateral incisors, and those at labial and lingual portion below root apex were most frequently observed between central incisors. The mean diameters of nutrient canals were 0.54 mm at labial portion above root apex, 0.61 mm at lingual portion above root apex, 0.66 mm at labial portion below root apex, and 0.76 mm at lingual portion below root apex. The mean lengths of nutrient canals were 2.63 mm at labial portion above root apex, 3.74 mm at lingual portion above root apex, 4.51 mm at labial portion below root apex, and 6.77 mm at lingual portion below root apex. Conclusion : CBCT is useful device to evaluate the anatomical structure of nutrient canals on mandibular anterior region.

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$CO_2$ 농도의 상승과 온난화환경이 수도의 생장, 물질생산 및 그 분배에 미치는 영향 (Effects of Elevated $CO_2$ and Global Warming on Growth Parameters, Biomass Production and Its Partitioning of Rice)

  • 김한용
    • 한국자원식물학회지
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    • 제11권1호
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    • pp.80-85
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    • 1998
  • The influence of elevated CO2 and temperature on growth parameters, biomass production and its partitioning of rice (Oryza sativa L.cv. Chukwangbyeo) were investigated in the three experiments (1991-1993). Rice plants were grown from transplanting to harvest at either ambient(350ppm) or elevated CO2 concentrations (690 or 650ppm) in combination with either four or seven temperature regimes ranging form ambient temperature (AT) to AT plus 3$^{\circ}C$.From transplanting to panicle initiation, crop growth rate (CGR) was enhanced by up to 27% with elevated CO2 , primarily due to an an increase in leaf area index. although net assimilatiion rate was also greater at elevated CO2. The effect of elevated CO2 varied with temperature. During the reproductive phase, CGR declined linearly with increased temperature, and was greater at elevated CO2 . Elevated CO2 increased final crop biomass and panicle weight 30% respectively at AT(27.6$^{\circ}C$ : 1991) . However, there was no significant effect of elevated CO2 on panicle weight at AT plus 3$^{\circ}C$, where severe spikelet sterility occurred. There was no significant effect of elevated CO2 on panicle weight at AT plus 3$^{\circ}C$, where severe spikelet sterility occurred. There was also no effect of CO2 on biomass pratitioning into vegetative and reproductive organs (harvest index)) at AT, although higher temperature could affect that by inducing spikelet sterility. These results suggest that elevated CO2 could enhance rice producivity througth promoted growth and biomass production , but its positive effects may be less at higher temperatures.

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애기장대 AtPGR 단백질의 병 저항성에 관한 생리적 특성 분석 (Physiological Characterization of an AtPGR from Arabidopsis Involved in Pathogen Resistance)

  • 정문수;김철수
    • 생명과학회지
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    • 제21권9호
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    • pp.1295-1300
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    • 2011
  • 식물체에서 당 조절 인자의 병 저항성 생리적 특성을 살펴보길 위하여, 원형질막에 존재하는 glucose 조절인자인 애기장대 AtPGR 유전자의 과발현 및 RNAi 형질전환체를 사용하였다[3]. AtPGR 유전자는 병원균 처리에 의하여 전사 발현양이 증가하였을 뿐만 아니라, JA와 SA 처리 시에도 AtPGR 전사 발현양이 증가함을 확인하였다. 과발현 형질전환체를 이용하여 병 저항성을 살펴본 결과, AtPGR 유전자는 병원균에 대해 저항성을 유도함을 알수 있었다. 또한 병원균 유도 증가 유전자로 알려진 PDF1.2 및 PR1 유전자 발현 양상을 qPCR을 통해 살펴본 결과, AtPGR 유전자는 PDF1.2 유전자를 SA 경로 하에서는 증가시키는 반면, JA 경로 하에서는 발현 증가량을 감소시키는 경향이 있음을 나타내었고, PR1 유전자의 발현은 JA 경로를 통해 조절할 것으로 생각되어진다. 이러한 결과를 바탕으로, AtPGR 유전자는 glucose 뿐만 아니라 병원균 반응에도 관련되어져 있음을 알 수 있다.

임의의 경계조건을 갖는 철근 콘크리트 사판의 해석 (An Analysis of the R/C Skew-Plates With Arbitrary Boundary Conditions)

  • 조현영;조진구
    • 한국농공학회지
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    • 제28권4호
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    • pp.49-56
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    • 1986
  • This study was carried out to investigate mechanical characteristics of the uniformly loaded skew-plate at 4 kinds of boundary condition : i) all edges are clamped (BC-1) , ii) all edges are simply supported (BC- 2), iii) two opposite edges are clamped and the other two edges are free (BC-3), and iv )two opposite edges are simply supported and the other two edges are free (BC-4). Various skew angles, 0$^{\circ}$, 10$^{\circ}$, 15$^{\circ}$, 30$^{\circ}$, 40: 45: and 60, of the plate were tested for the above boundary conditions. Resutts obtained from the study are summarized as follows ; 1.The lateral displacement at the center of a skew- plate was decreased as the skewangle increased at all of the boundary conditions. The decrements of the conditions of BC-3 and BC-4 were considerable. And, difference of the displacement between the boundary conditions was decreased as the skew-angle was increased. 2. X-moments (to the Y-axis) at the center of a skew- plate and the minimum principal moments were shown as a similar pattern of change with respect to the skew-angle variation between BC-i and BC-2 and between BC-3 and BC-4, and the pattern of change at the conditions of BC-3 and BC-4 were shown higher rates than those for the conditions of BC-i and BC-2 3.Y-moments (to the X- axis) at the center of a skew-plate and the maximum principal moment were decreased as the skew-angle increased in a similar pattern at all of the boundary conditions. 4.X-moments at the obtuse angle side of a skew-plate were shown as a parabolic pattern of change (frist increased after then decreased) as the skew-angle increased, and a skew-angle resulting the maximum absolute moment was depended on the boundary conditions. 5.Y-moments at the obtuse angle side of a skew-plate were affected by the skewangle much more at the boundary condtions of BC-2 and BC-4 than at the conditions of BC-i and BC-3. 6.Maximum principal moments at the obtuse angle side of a skew-plate at the skew angle of 40$^{\circ}$- 45$^{\circ}$ were resulted almost the same value at all of the boundary conditions .

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양식산 참조기(Larimichthys polyactis) (민어과) 자치어의 골격발달 (Osteological Development of Larvae and Juveniles of the Cultured Small Yellow Croaker Larimichthys polyactis (Sciaenidae))

  • 이강현;조정현;김진구
    • 한국수산과학회지
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    • 제56권2호
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    • pp.228-242
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    • 2023
  • We examined the osteological development of the cranium, visceral skeleton, pectoral girdle, vertebral column, fins, and pterygiophore of small yellow croaker Larimichthys polyactis cultured at the Jeju Fisheries Research Institute. At 5.89 mm NL (notochord length), the larvae already had ossified frontal and basioccipital bones, parasphenoids, and vomer in the cranium. All elements of the visceral skeleton except the metapterygoid were ossified at 5.89 mm NL. Preorbital bone was ossified at 8.11 mm SL (standard length), and suborbital bones were ossified at 12.90 mm SL. In the vertebral column, ossification proceeded from the first to last centrum. The urostyle ossified at 7.41 mm SL. The caudal skeleton ossified at 6.78 mm NL, along with three hypural bones. In the pectoral girdle, the cleithrum, supracleithrum, postcleithrum, and post-temporal bones ossified at 5.89 mm NL. Caudal fin ossification began at 6.19 mm SL and was complete at 7.16 mm SL. Dorsal and anal fin ossification began at 7.16 mm SL and was complete at 8.62 mm and 8.11 mm SL, respectively. Anal and dorsal pterygiophore bone ossification was complete at 9.86 mm and 11.54 mm SL, respectively, and that of the supraneural bones was complete at 9.60 mm SL.