• 제목/요약/키워드: ASED

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DNA Barcoding of the Marine Proteced Species Pseudohelice subquadrata (Decapoda, Varunidae, Pseudohelice) from the Korean Waters

  • Kim, Ji Min;Kim, Jong-Gwan;Kim, So Yeon;Choi, Woo Yong;Kim, Hyung Seop;Kim, Min-Seop
    • Animal Systematics, Evolution and Diversity
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    • 제36권3호
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    • pp.228-231
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    • 2020
  • Pseudohelice subquadrata (Dana, 1851) is endangered due to its restricted habitat; hence, it has been designated as a marine protected species and endangered species by law in Korea. It has been recorded only Jeju-do and Geomun-do, Republic of Korea. The present study, is the first report on a cytochrome c oxidase subunit I DNA barcode for P. subquadrata. The maximum intra-specific genetic distance among all P. subquadrata individuals was found to be 0.5%, whereas inter-genetic distance within the same genus was 17.2-21.5% compared with Helice tientsinensis (Rathbun, 1931), H. tridens (De Haan, 1835), H. epicure (Ng et al., 2018), and Helicana wuana (Rathbun, 1931). Our barcoding data can thus be used as reference for restoration and conservation studies on P. subquadrata, which are designated as marine protected species.

First Report of Five Tobrilus Species (Nematoda: Triplonchida) from Korea

  • Kim, Jiyeon;Kim, Taeho;Yu, Jeong-Nam;Park, Joong-Ki
    • Animal Systematics, Evolution and Diversity
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    • 제36권3호
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    • pp.240-250
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    • 2020
  • Member of the genus Tobrilus Andrássy, 1959, which belongs to Tobrilidae Filipjev, 1918, are known as free-living nematodes in freshwater habitat. This genus was previously unknown from Korea. Five Tobrilus species are here reported for the first time from Korea: Tobrilus aberrans (Schneider, 1925), Tobrilus diversipapillatus (Daday, 1905), Tobrilus gracilis (Bastian, 1865), Tobrilus longus (Leidy, 1851), and Tobrilus wesenbergi (Micoletzky, 1925). Specimens were collected from sediments of the Nakdong River in Korea. Morphological characters and measurements of the specimens generally agree with the original descriptions of Tobrilus species, except for some differences that can be attributed to intraspecific variation among populations(e.g., nerve ring position [% pharynx] and reproductive length). Each species can be distinguished from other members of the genus by specific characters (e.g., cephalic setae length and position, buccal cavity and pocket shape, vulva position, degree of development of reproductive system, and tail length and shape). Here, five species in the genus Tobrilus are fully redescribed and illustrated using optical microscopy images. DNA barcode sequence information (the D2-D3 region of 28S rDNA) is also provided for molecular species identification.

First Report of Two Plectus Species (Nematoda: Plectida) from Korea

  • Kim, Jiyeon;Yu, Jeong-Nam;Kim, Taeho
    • Animal Systematics, Evolution and Diversity
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    • 제36권3호
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    • pp.251-257
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    • 2020
  • The genus Plectus Bastian, 1865 represents a group of free-living freshwater nematodes belonging to the family Plectidae Örley, 1880. However, only one species has been reported thus far from Korea. Plectus aquatilis Andrássy, 1985 and Plectus cirratus Bastian, 1865 are reported for the first time from Korea, from sediments collected from the Nakdong River. Plectus aquatilis is distinguished from other Plectus species by its three longitudinal alae in the lateral field, thin and directed cephalic setae, continuous lip region (head not set-off), and rectangular shaped tail. Plectus cirratus is distinguished from other Plectus species by its large body, two longitudinal alae in the lateral field, larger stoma, and longer tail. Morphological characters and measurements of the specimens generally agree with the original descriptions of Plectus species. Here, the morphological characters (detailed morphometrics) of two species in the genus Plectus are fully redescribed and illustrated using optical microscopy. DNA barcode sequence information from the 18S rDNA gene is also provided for molecular species identification and compared with sequences from other Plectus species available on GenBank.

Comparison of Adsorption Properties of Adsorbates on Pt(111) and Pt(111)/$\gamma-Al_2O_3$ Surface in the Ethylene Hydrogenation Reaction : MO-Theory

  • 조상준;박상문;박동호;허도성
    • Bulletin of the Korean Chemical Society
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    • 제19권7호
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    • pp.733-737
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    • 1998
  • Using an atom superposition and electron delocalization molecular orbital (ASED-MO) method, we have compared adsorption properties of adsorbates on the Pt(Ill) surface with the Pt(lll)/γ-Al203 surface in the ethylene hydrogenation reaction. In two-layer thick model systems, the calculated activation energy of the hydrogenation by the surface platinum hydride is equal to the energy by the hydride over supported platinum/γ-alumina. The transition structure on platinum is very close to the structure on the supported platinum/γ-alumina surface. Hydrogenation by the surface hydride on platinum can take place easily because the activation energy is about 0.5 eV less than hydrogenation by ethylidene. On supported platinum/,y-alumina the activation energy of the hydride mechanism is about 0.61 eV less than that of ethylidene mechanism. In one-layer thick model systems, the activation energy of hydrogenation by ethylidene is about 0.13 eV less than the activation energy of hydride reaction. The calculated activation energy by the hydride over the supported platinum y-alumina is 0. 24 eV higher than the platinum surface. We have found from this result that the catalytic properties of one-layer thick model systems have been influenced by the support but the two-layer thick model systems have not been influenced by the support.

Seven New Records of Geometrid Moths (Lepidoptera: Geometridae) from Korea

  • Choi, Sei-Woong;Kim, Sung-Soo;Heo, Un-Hong
    • Animal Systematics, Evolution and Diversity
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    • 제33권2호
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    • pp.123-130
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    • 2017
  • The family Geometridae, which is one of the most species-rich taxa of Lepidoptera, includes more than 23,000 species worldwide and over 800 species in South Korea. Herein, we record six species of Geometridae for the first time in Korea. Jodis urosticta and Jodis amamiensis can be characterized by their greenish wings and strongly dentate ante- and postmedial lines on the fore and hindwings. These species can also be separated by the shape of the valva of the male genitalia. Pelagodes antiguadraria can be characterized by their greenish wings, whitish medial bands on the forewing and whitish postmedial line on the hindwing. Brabira kasaii can be characterized by the thick grayish band-shaped central fascia of their fore- and hindwings. Sauris marginepunctata can be characterized by the greenish ground color of the forewing, the dark ochreous medial bands and subterminal line and the grayish hindwing. Philereme vetulata can be characterized by their dark brownish wing color and the band-shaped central fascia of the forewing, as well as the medially projected postmedial line of the hindwing. Echthrocollix minuta can be characterized by their pale grayish wing color, brownish postmedial and subterminal lines and large discal dot.

Development and Characterization of 10 Polymorphic Microsatellite Loci in the Korean Endemic Freshwater Fish Iksookimia koreensis, and Their Cross-species Amplification in the Endemic I. longicorpa

  • Kwan, Ye-Seul;Kim, Hyo-Jin;Lee, Bit-Na;Won, Yong-Jin
    • Animal Systematics, Evolution and Diversity
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    • 제33권2호
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    • pp.136-139
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    • 2017
  • The genus Iksookimia (Actinopterygii: Cypriniformes: Cobitidae) is a bottom-dwelling freshwater loaches, which are well-known as their endemism and high geographic variation. However, population genetic relationships among Iksookimia spp. have remained unclear due to a shortage of genetic markers that can be applied generally in the genus. Here, we developed high-resolving microsatellite markers using I. koreensis and I. longicorpa as representatives of Iksookimia species because of their wide distribution range and phylogenetic position. Ten of polymorphic microsatellite loci were isolated from Iksookimia koreensis and were successfully cross-amplified in I. longicorpa. The mean number of observed alleles per locus was about 10.4 (range, 2-17) for I. koreensis and about 13.2 (range, 2-24) for I. longicorpa. The loci, IK03 and IK08, deviated from the Hardy-Weinberg equilibrium in I. koreensis, after applying the Bonferroni correction. The microsatellite markers obtained in the present study will be useful to evaluate population genetic structure and to establish conservation strategies for I. koreensis and related Iksookimia species.

Three Records of the Genus Tubastraea (Anthozoa: Hexacorallia: Scleractinia: Dendrophylliidae) from Korea

  • Choi, Eunae;Song, Jun-Im
    • Animal Systematics, Evolution and Diversity
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    • 제33권2호
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    • pp.65-72
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    • 2017
  • This study reports three species in the genus Tubastraea from Korea: Tubastraea coccinea Lesson, 1829; Tubastraea faulkneri Wells, 1982; and Tubastraea micranthus (Ehrenberg, 1834). Tubastraea faulkneri and T. micranthus are newly recorded in Korea. The specimens of three species were collected in the subtidal zones off Jeju-do between 1991 and 2010. The two newly recorded species were described in detail based upon the morphological characters of skeletal structures. The previous records of T. coccinea in Korea were supplemented with additional data in the remarks. These three species have a straight septal arrangement or irregular septal fusion in common as a main character for the genus Tubastraea, but they differ with respect to the growth form, intercorallite distance, exsertness from common coenosteum, and the detailed characters of septal arrangement. Tubastraea faulkneri is similar to T. coccinea in its plocoid growth form, well developed common coenosteum, and corallite size. However, unlike the latter species, the former species is characterized by rare or absent budding adjacent to the corallite edges, wider intercorallite distance, and irregularly developed septal fusion near the columella. In particular, T. micranthus is distinguished by an axial dendroid growth form, and the smallest corallites in this genus.

Development and Characterization of 14 Microsatellite Markers for the Antarctic Midge Parochlus steinenii (Diptera, Chironomidae) in Maritime Antarctic

  • Kim, Hanna;Kang, Seunghyun;Kim, Hanul;Kim, Sanghee;Jung, Jongwoo
    • Animal Systematics, Evolution and Diversity
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    • 제33권2호
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    • pp.140-143
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    • 2017
  • A winged midge species, Parochlus steinenii is one of the most abundant species in Antarctica, which is distributed over a wide area from the South American continent to the South Shetland Islands in Antarctica. It was dispersed into islands in the South Shetland Islands from the South American continent, and it adapted to a variety of environments and settled. This species, therefore, is a good model organism to explain the evolutionary process of Antarctic terrestrial fauna. Nevertheless, there are few genetic studies on this species, which are necessary for understanding the genetic diversity, population structure, etc. Here, we developed and characterized 14 polymorphic microsatellite markers. The number of alleles per locus ranged from 2 to 5. The observed and expected heterozygosities were in the range of 0.024 to 0.561 and 0.024 to 0.535, respectively. Identifying genetic differences between populations, they are suitable markers for researches investigating genetic diversity and population structure of P. steinenii, which provide us with clues to dispersion, evolution and ecology of this species.

Additional Records of the Hydrothermal Vent Scale Worm Branchinotogluma segonzaci (Polynoidae: Lepidonotopodinae) from the North Fiji Basin and Tonga Arc

  • Lee, Won-Kyung;Lee, Geon Hyeok;Ju, Se-Jong;Kim, Se-Joo
    • Animal Systematics, Evolution and Diversity
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    • 제37권4호
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    • pp.273-279
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    • 2021
  • Branchinotogluma segonzaci (Miura and Desbruyères, 1995) occurs in hydrothermal vent fields of the southwestern Pacific Ocean. We morphologically compared B. segonzaci from the North Fiji Basin with the original description from the Lau Basin and a subsequent study of specimens from the Manus Basin. The main characteristics of all B. segonzaci populations were similar having 21 segments, 10 pairs of elytra, cylindrical-shaped anterior lobes, and ventral papillae on segment 12 and ventral lamellae on segments 13-17 in males. However, the specimens from the North Fiji Basin had rounded to sub-renifrom elytra rather than oval in the original description. Additionally, we newly obtained 11 cytochrome oxidase subunit I (COI) DNA barcodes from the North Fiji Basin and Tonga Arc populations and compared them with known COI DNA barcodes of Branchinotogluma species. Thirteen sequences of B. segonzaci showed 0.0-1.07% intraspecific variation and formed two clades in the COI neighbor-joining tree, whereas the interspecific variation among Branchinotogluma species was 8.19-22.4%. The results of this study contribute to biogeographic studies of B. segonzaci and the evolution of polynoid scale worms in chemosynthesis-based ecosystems.

First Record of the Interstitial Annelid Pharyngocirrus uchidai (Annelida: Saccocirridae) from Korea, Confirmed by Topotypic DNA Barcoding Data from Japan

  • Park, Jiseon;Kajihara, Hiroshi;Jung, Jongwoo
    • Animal Systematics, Evolution and Diversity
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    • 제35권1호
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    • pp.33-36
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    • 2019
  • The marine interstitial annelid Pharyngocirrus uchidai(Sasaki, 1981) has been only known from Japan. In this study, we report the occurrence of P. uchidai for the first time in four localities along the eastern coast of Korea: Bukmyeon, Gamchu, Gase, and Oeongchi. Species identification was confirmed by comparison of DNA barcoding sequences with morphological examination from the type locality, Oshoro, Japan. We generated a total of 25 sequences of a partial segment (580 bp) of the cytochrome c oxidase subunit I gene (COI), representing five specimens from each locality. Maximum intra-specific variation was 1.2% in terms of Kimura two-parameter (K2P) distance, observed between two individuals each from Gamchu (i.e., between two specimens from the single locality), Gamchu and Oeongchi, Gamchu and Oshoro, and Oeongchi and Oshoro. On the other hand, an identical haplotype was found in all the five localities, substantiating our species identification for the Korean populations. Inter-specific K2P distance between P. uchidai and an unidentified Saccocirrus sp. from Canada (based on public database entries) was 22.4-23.4%.