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A study of image evaluation and exposure dose with the application of Tube Voltage and ASIR of Low dose CT Using Chest Phantom (흉부 Phantom을 이용한 Low Dose CT의 관전압과 ASIR(Adaptive Statistical Iterative Reconstruction)적용에 따른 영상평가 및 피폭선량에 관한 연구)

  • Hwang, Hyeseong;Kim, Nuri;Jeong, Yoonji;Goo, Eunhoe;Kim, Kijeong
    • Korean Journal of Digital Imaging in Medicine
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    • v.16 no.2
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    • pp.9-14
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    • 2014
  • Purpose: The purpose of this study has attempted to evaluate and compare the image evaluation and exposure dose by respectively applying Filtered Back Projection(FBP), the existing test method, and Adaptive Statistical Iterative Reconstruction(ASIR) with different values of tube voltage during the Low Dose Computed Tomography(LDCT). Materials and Methods: With the image reconstruction method as basis, Chest Phantom was utilized with the FBP and ASIR set at 10%, 20% respectively, and the change of Tube Voltage (100kVp, 120kVp). For image evaluation, Back ground noise, Signal to Noise ratio(SNR) and Contrast to Noise ratio(CNR) were measured, and, for dose evaluation, CTDIvol and DLP were measured respectively. The statistical analysis was tested with SPSS(ver. 22.0), followed by ANOVA Test conducted after normality test and homogeneity test. (p<0.05). Results: In terms of image evaluation, there was no outstanding difference in Ascending Aorta(AA) SNR and Infraspinatus Muscle(IM) SNR with the different values of ASIR application(p<0.05), but a significant difference with the different amount of tube voltage(p>0.05). Also, there wasn't noticeable change in CNR with ASIR and different amount of Tube Voltage (p<0.05). However, in terms of dose evaluation, CTDIvol and DLP showed contrasting results(p<0.05). In terms of CTDIvol, the measured values with the same tube voltage of 120kVp were 2.6mGy with No-ASIR and 2.17mGy with 20%-ASIR respectively, decreased by 0.43mGy, and the values with 100kVp were 1.61mGy with No-ASIR and 1.34mGy with 20%-ASIR, decreased by 0.27mGy. In terms of DLP, the measured values with 120kVp were $103.21mGy{\cdot}cm$ with No-ASIR and $85.94mGy{\cdot}cm$ with 20%-ASIR, decreased by $17.27mGy{\cdot}cm$(about 16.7%), and the values with 100kVp were $63.84mGy{\cdot}cm$ with No-ASIR and $53.25mGy{\cdot}cm$ with 20%-ASIR, a decrease by $10.62mGy{\cdot}cm$(about 16.7%). Conclusion: At lower tube voltage, the rate of dose significantly decreased, but the negative effects on image evaluation was shown due to the increase of noise. For the future, through the result of the experiment, it is considered that the method above would be recommended for follow-up patients or those who get health checkup as long as there is no interference on the process of diagnosis due to the characteristics of Low Dose examination.

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An Ultrastructural Study on the Effect of Exogenous $Ca^{2+}$ Stimulation to Ischemic Myocardium during Post-ischemic Reperfusion (재관류시 외인성 칼슘 자극이 허혈 심근에 미치는 영향에 대한 전자현미경적 연구)

  • Kim, Ho-Dirk;Chun, Sang-Bae;Rah, Bong-Jin
    • Applied Microscopy
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    • v.21 no.1
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    • pp.1-20
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    • 1991
  • The effects of exogenous $Ca^{2+}$ stimulation on the post-ischemic myocardial cells were studied using isolated Langendorff-perfused guinea pig hearts. At the starting point of reperfusion, Tyrode solutions, each containing 2.0mM, 4.0mM and 8.0mM $CaCl_2$ respectively, were administered for 2 minutes apart by descending, ascending, or by combined sequences followed by standard Tyrode solution containing 1.0mM $CaCl_2$. The minutes of global ischemia produced reversible but moderate to severe degree of myocardial ultrastructrual changes including focal destruction of sarcolemma, loss of nuclear matrix, clumping and margination of chromatins, mitochondrial swelling, destruction of mitochondrial cristae, shortening of sarcomeres, focal loss of myofibrils, and separation of cell junctions. In spite of reperfusion, the ultrastructure was more severely damaged and irreversible changes such as intracellular fluid accumulation, contracted sarcomeres, mitochondrial destruction, disruption of sarcolemma, loss of nuclear matrix, and separation of cell junction were observed in a large number of cells. In contrast, Tyrode-perfused $Ca^{2+}$-stimulated myocardial cells showed relatively well preserved ultrastucture, except slight changes including focal mitochondrial swelling, widening of T-tubule, and widening of cell junctions, especially at fasciae adherentes. The post-ischemic $Ca^{2+}$-stimulated reperfused myocardial cells produced focal changes such as mitochondrial destruction, disintegration of sarcolemma, widening of T-tubule, and intracellular fluid accumulation with slight variation in degree of changes by the method of $Ca^{2+}$ administration sequence. However, in a large number of the myocardial cells, chromatins were redistributed relatively evenly in the nuclear matrix, mitochondrial cristae were tightly packed, and a considerable number of intramitochondrial granules and glycogen granules reap-pealed. These results indicate that exogenous $Ca^{2+}$ stimulation in the initial period of reperfusion may be beneficial to salvage or to reduce the post-ischemic myocardium from further deleterious changes, and that the beneficial effects may be derived from the reserves of the function of the intracellular $Ca^{2+}$ regulating organelles and/or from the responsiveness of contractile apparatus to $Ca^{2+}$ stimulation.

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Isolation and Purification of Berberine in Cortex Phellodendri by Centrifugal Partition Chromatography (Centrifugal Partition Chromatography에 의한 황백으로부터 Berberine의 분리 및 정제)

  • Kim, Jung-Bae;Bang, Byung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.27 no.3
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    • pp.532-537
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    • 2014
  • Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections, and infections of the body surface. Many analytical methods have been used to study oriental herbal medicines, such as thin-layer chromatography, column liquid chromatography, and high performance liquid chromatography (HPLC). In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out in order to separate pure compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 mL/min in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. The $^1H$ NMR spectrum revealed that the resonances at ${\delta}$ 4.10 and 4.20 ppm corresponded to three protons ($-OCH_3$), whereas those at ${\delta}$ 6.10 ppm corresponded to two protons ($-OCH_2O-$). Further, two aromatic protons (H-11 and H-12) conveys a doublet-doublet pattern. The H-11 doublet and H-12 doublet appear at ${\delta}$ 7.98 and 8.11, respectively. The $^{13}C$ NMR. spectrum showed a tetrasubstituted with a methylenedioxy group at C2 and C3, and two methoxy groups at C9 and C10. The chemical structure of the berberine was identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.

The Neuroanatomy and Psychophysiology of Attention (집중의 신경해부와 정신생리)

  • Lee, Sung-Hoon;Park, Yun-Jo
    • Sleep Medicine and Psychophysiology
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    • v.5 no.2
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    • pp.119-133
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    • 1998
  • Attentional processes facilitate cognitive and behavioral performance in several ways. Attention serves to reduce the amount of information to receive. Attention enables humans to direct themselves to appropriate aspects of external environmental events and internal operations. Attention facilitates the selection of salient information and the allocation of cognitive processing appropriate to that information. Attention is not a unitary process that can be localized to a single neuroanatomical region. Before the cortical registration of sensory information, activation of important subcortical structures occurs, which is called as an orienting response. Once sensory information reaches the sensory cortex, a large number of perceptual processes occur, which provide various levels of perceptual resolution of the critical features of the stimuli. After this preattentional processing, information is integrated within higher cortical(heteromodal) systems in inferior parietal and temporal lobes. At this stage, the processing characteristics can be modified, and the biases of the system have a direct impact on attentional selection. Information flow has been traced through sensory analysis to a processing stage that enables the new information to be focused and modified in relation to preexisting biases. The limbic and paralimbic system play significant roles in modulating attentional response. It is labeled with affective salience and is integrated according to ongoing pressures from the motivational drive system of the hypothalamus. The salience of information greatly influences the allocation of attention. The frontal lobe operate response selection system with a reciprocal interaction with both the attention system of the parietal lobe and the limbic system. In this attentional process, the search with the spatial field is organized and a sequence of attentional responses is generated. Affective, motivational and appectitive impulses from limbic system and hypothalamus trigger response intention, preparation, planning, initiation and control of frontal lobe on this process. The reticular system, which produces ascending activation, catalyzes the overall system and increases attentional capacity. Also additional energetic pressures are created by the hypothalamus. As psychophysiological measurement, skin conductance, pupil diameter, muscle tension, heart rate, alpha wave of EEG can be used. Event related potentials also provide physiological evidence of attention during information process. NI component appears to be an electrophysiological index of selective attention. P3 response is developed during the attention related to stimulus discrimination, evaluation and response.

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Electron-microscopic studies on fine structure and enzyme activity in the axenic and conventional strains of Entamoeba histolytica (이질아메바(Entamoeba histolytica)의 미세구조 및 효소활성에 관한 전자현미경적 연구)

  • Yong, Tae-Sun;Jeong, Pyeong-Rim;Lee, Geun-Tae
    • Parasites, Hosts and Diseases
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    • v.23 no.2
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    • pp.269-284
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    • 1985
  • The metabolism of Entamoeba histolytica would be affected by various environmental factors, and alteration of the environment was known to afEect the fine structure of 5. histolytica. The present study was designed electronmicroscopically to investigate the ultrastructure and enzyme activities in the aEonic and conventional strains of 5. histolytica. The trophozoites of axenically cultivated HK-9 strain and conventional YS-27 and YS-49 strains of 5. histolytica were collected and liKed with 4% paraformaldehyde/0.1M cacodylate buffier(pH 74), After washing them by centrifugation, 1% warm agar was added in the sediment. Solidified agar with the trophozoites was cut into $lmm^3$ cubes, and incubated in the various substrates to observe enzyme activities. Then, the specimen was post-fixed with 3% glutaraldehyde/0.1M cacodylate buffer (PH 7.4) and 1% osmium tetroBide/0.1M cacodylate buffier (pH 7.4) , dehydrated in ascending ethanol series and embedded in epoxy resin. These were sectioned on an ultramicrotome and observed with a transmission electronmicroscope. The procedures for the observation of the fine structure were same as the above, except for the incubation in the substrate. The sections were stained with uranyl acetate and lead citrate. For the observation of the surface of the amoebae, scanning-electronmicroscopy was carried out. The results obtained in the present study are summarized as follows: 1. The fuzzy coat around double-layered plasma membrane of 5. histolytica was more irregularly and densely distributed in the conventional strains (YS-27, YS-49 strains) than in the axonic strain (HK-9 strain). 2. The endosomes, button bodies and chromatin material were surrounded by a double-layered nuclear membrane having scattered nuclear fores. The paranuclear body, mono- or double-layered vacuoles, vacuolar membrane whorls, rosette-like cylindrical bodies, aggregation of cylindrical bodies and helical bodies were found in the cytoplasm of the amoebae. Helical bodies and glycogen granules were generally abundant, while a few smooth endoplasmic reticula were observed in the cytoplasm. 3. Alkaline phosphatase activity was mainly demonstrated in the plasma membrane, limiting membranes of vacuoles and smooth endoplasmic reticula. ATPase activity was observed in the nucleus, limiting membranes of vacuoles and vacuolar membrane whorls. 4. Acid phosphatase activity was commonly demonstrated in the limiting membranes an contents of vacuoles, Iysosome-like organelles, plasma membrane and the button bodies in the nucleus. The activity was more weakly demonstrated in the HK-9 strain than in the other conventional strains of 5. histolytica. No peroBidase activity was observed in the amoeba strains employed in the present study. 5. With a scanning electron-microscope, no distinct structural differences were observed between the amoeba strains. All the trophozoite forms of the amoebae showed crater-like depressions and rugged features on the outer surface.

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Two Newly Naturalized Species in Korea: Lolium rigidum Gaudin (Poaceae) and Oenothera rosea L'Hér. ex Aiton (Onagraceae) (한반도 미기록 귀화식물: 댕돌보리와 애기분홍낮달맞이꽃)

  • Kim, Jung-Hyun;Kim, Jin-Seok;Nam, Gi-Heum;Yoon, Chang-Young;Kim, Sun-Yu
    • Korean Journal of Plant Resources
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    • v.27 no.4
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    • pp.326-332
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    • 2014
  • Two unrecorded naturalized species, Lolium rigidum Gaudin (Poaceae) and Oenothera rosea L'H$\acute{e}$r. ex Aiton (Onagraceae) were newly reported in Korea. L. rigidum, 'Daeng-Dol-Bo-Ri', was found at roadside of Yesong beach, Yesong-ri, Bogil-myeon, and roadside of Myeongsasimni beach, Sin-ri, Sinji-myeon, in Wando-gun, Jeollanam-do. It was distinguished from L. mutiflorum Lam by spikelet 10-20 mm long, florets 2-10(-13), and obtuse lemma, awn absent or up to 3-8(-10) mm. O. rosea, 'Ae-Ki-Bun-Hong-Nat-Dal-Mat-I-Kkot', was found in side of Wondong wetland, Yongdang-ri, Wondong-myeon, Yangsan-si, Gyeongsangnam-do. This taxon was distinguishable from other Oenothera in Korea by following combination of characters, stem erect to ascending, petal 5-12 mm long, pink to bright purple. Descriptions and illustrations of the two taxa and their photographs in the habitat are provided along with a key to the species of Lolium and Oenothera from Korea.

The Clinical Results of Open Heart Surgery with Coronary Artery Bypass Grafting (관상동맥 우회로 조성수술을 병행한 개심수술의 임상성적)

  • 유경종;강면식
    • Journal of Chest Surgery
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    • v.29 no.2
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    • pp.171-176
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    • 1996
  • From March, 1983 to June, 1994, twenty-two patients underwent coronary artery and combined operations. The ages of the patients ranged from 42 years to 72 years (mean 60.4$\pm$8.2 years). There were 17 male and 5 female patients. The left ventricular (LV) ejection fraction ranged from 25% to 65% (mean 46.9$\pm$14.2%). Nine patients had mechanical complication of myocardial infarction (MI), of which 5 were LV aneurysm, 3 ventricular septal defect and 1 mitral regurgitation. Nine patients had rheumatic valvular heart disease of whom 7 with aortic valve disease and 2 with mitral valve disease. Two other patients had left atrial thrombi, only one with atrial septal defect a d another with aneurysm of ascending aorta. An average of 2.1$\pm$1.0 bypasses was done, ranging from one to four. There were 3 postoperative complications; 2 perioperative MI and 1 leg wound infection. Among complicated patients, mortality was 1 patient (4.5%) due to low cardiac output syndrome after perioperative MI. With 3 to 136 months follow-up (mean 41.1$\pm$40.2 months), late mortality was 1 patient due to cerebral vascular accident. Among long-term survivors, all patients are in New York Heart Association functional class I or II. Although the number of patients was small, our surgical results were favorable. Therefore we think that coronary revascularization combined with heart operation does not increase the operative risk when associated coronary artery disease is present, and it reduces the occurrence of late death.

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The genesis of Ulsan carbonate rocks: a possibility of carbonatite\ulcorner (울산 광산에 분포하는 탄산염암체의 성인에 관한 연구: 카보내타이트의 가능성)

  • 양경희;황진연;옥수석
    • The Journal of the Petrological Society of Korea
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    • v.10 no.1
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    • pp.1-12
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    • 2001
  • A small of carbonate rocks and spatially-associated ultramafic rocks uniquely occur in the ulsan iron-serpentine mine of the sourtheastern Kyungsang basin. The study of field geology, core drilling data and stable isotope analysis suggest that the carbonate rocks are carbonatite formed from the melt reflecting intrusive natures. Based on this study, the geology of the Ulsan iron-serpentinite mining area consists of Cretaceous sedimentary, volcanic, granitic ultramafic and carbonate rocks in ascending order. The carbonate and ultramafic rocks show concentric and ellipsoidal shapes at the outcrop and a funnel shape in the cross sectional view. Carbon and oxygen stable isotope analysis show a bimodal pattern rather than a typical mantle pattern, which may indicate that the melt was a secondary melt generated within the crus not in the mantle directly. The uprising of ultramafic melts would have melted lime-contained rocks forming a secondary carbonate melt in the upper crus. Then, the intrusion of the ultramafic melts would have melted lime-contained rocks forming a secondary carbonate melt in the upper crust. Then, the intrusion of the ultramafic melt was followed by the intrusion of the carbonate melt along deep-seated fractures. Well-developed major fractures in this area, fluid inclusion characteristics of the carbonate rocks, the spatial relation between the ultramafic and carbonate rocks and stable isotope data support interpreting the Ulsan carbonate rocks as carbonatite.

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Type and Evolution of the Myeonbongsan Caldera in Southern Cheongsong, Korea (청송남부 면봉산 칼데라의 유형과 진화)

  • 황상구;김성규
    • The Journal of the Petrological Society of Korea
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    • v.8 no.3
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    • pp.171-182
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    • 1999
  • The Myeonbongsan caldera, 10.2X8.0 km, developed within older sequences of sedimentary formations and intermediate composition volcanis in the southern Cheongsong area. Volcanic rocks in the caldera block include lower intermediate volcanics, middle tuffaceous sequences and upper silicic ones. The silicic volcanics, which is named Myeonbongsan Tuff, are composed of crystal-rich ash-flow tuff(300 m) , bedded tuff(30 m) and pumice-rich ash-flow tuff(700 m) in ascending order. Several intrusions dominate the early sequences within the caldera. The caldera collapsed in a trapdoor type when silicic ash-flow tuffs erupted fro major vent area in the caldera. Normal faulting along a ring fault system except the southwestern part dropped the tuffs down to the northrase with a maximum displacement of about 820 m. The Myeonbongsan Tuff is just about 1,030 m thick inside the northeastern caldera, with its base not exposed, and southwestward thinning down. Rhyolitic plug and ring dikes are emplaced along the central vent and the caldera margins, and the ring dikes are cut by plutonic stocks in the southeastern and northwestern parts. The caldera volcanism eviscerated the magma chamber by a series of explosive eruptions during which silicic magma was erupted to form the Myeonbongsan Tuff. Following the last ash-flow eruption, collapse of the chamber roof resulted in the formation of the Myeonbongsan caldera, a subcircular trapdoor-type depression subsiding about 820 m deep. After the collapse, stony to flow-banded rhyolites were emplaced as circular plugs and ring dikes along the central vent and the caldera margins respectively. Finally after the intrusions, another plutons were emplaced as stocks outside the caldera.

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Kinematic Analysis of Deff Motion in High Bars (철봉운동 Deff 동작의 운동학적 분석)

  • Back, Jin-Ho
    • Korean Journal of Applied Biomechanics
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    • v.16 no.1
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    • pp.55-63
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    • 2006
  • The purpose of this study is to prove the kinematical characteristics of Deff motion, the high bar performance, in terms of flying phases so that we can provide basic sources for improving gymnastic performance. To do this, we selected and analyzed the performance of two athletes who did Deff motion in the high bar competition of male artistic gymnastic in the 22nd Universiade 2003 Daegu. We drew the conclusions from the kinematical factors that were came out through analyzing three-dimensional cinematography of the athletes' movements, by using a high speed video camera. To make a successful performance, a performer releases the bar at a height of a high bar vertically and at a height of 82cm horizontally, and the flying performance should be made without moving forward, as maintaining the proper balance, in order to rise over 118cm high during the flying phase. When the performer is releasing the bar, an increase of the vertical speed in the center of the body and extension of a knee joint and a hip joint contribute to increasing a flying height. And when the moving body is twisted, leaning to left side is caused by the winding movement of a knee joint, which causes an unstable bar grasp. To grasp the bar stably, just before releasing the performer should gain propulsive force from twisting rotation through increasing the speed of shoulder rotation. And before the peak point, the performer should make sure of a body rotation distance over $164^{\circ}$ so that he or she can do an aerial rotary performance smoothly. When grasping the high bar, the center of the body should be above the bar and the angle of shoulder rotation should be maintained close to $540^{\circ}$ simultaneously. he high point performance(S1) has more speed on an ascending phase and less speed on a descending phase than the low point performance (S2). At the peak point, both the rotation angle of the body and that of the shoulder in high point performance are big as well. In conclusion, it is shown that a performer can make a jump toward the high bar easily with the body straight because the performer can hold the upper part of the body erect early in a descending phase.