• 대한치과기공학회지
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• 제37권1호
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• pp.9-15
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• 2015

Purpose: The purpose of this study was to compare the accuracy of the maxillary three-unit fixed dental prosthesis (FDPs) made using two CAD/CAM milling machines : DCM Group(Dentaim CAD/CAM milling machine), WCM Group(Wieland CAD/CAM milling machine). Methods: Each of 10 duplicate models was scanned by blue light scanner(Identica, Medit, Korea), and the three-unit FDPs (STL file) was designed using DelcamCAD. A total of 20 three-unit FDPs was fabricated, comprising 2 groups of 10 specimens each (shrinkage ratio is 1:1). The first three-unit FDPs STL file was used as a CAD reference model (CRM). Obtained STL files by scanning the inner surface of three-unit FDPs were convened into the point clouds-ASC II files. Discrepancies between the point clouds and CRM were measured by superimposition software. Statistical methods to analyze the data were used non-parametric method. The mean (SD) values were compared by a Mann-Whitney U-test. Type one error rate was set at 0.05. Results: WCM group had small discrepancies with $2.17{\mu}m$ of mean value compared to $4.44{\mu}m$ in DCM group. The accuracy values between the two groups showed a sratistically significant difference (Table 2, p<.05). Conclusion: The accuracy of the three-unit fixed dental prosthesis(FDPs) made of two CAD/CAM milling machines were statistically different. Accuracy with which the prosthesis made of WCM group was superior.

• 한국산림과학회지
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• 제95권6호
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• pp.768-777
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• 2006

이 연구는 지리산 국립공원 방문자를 대상으로 경관선호 모델을 형성하기 위하여, 지리산 경관과 영국의 케이른고럼스 경관의 선택적 상황에서 경관선호를 결정하게 하는 요소로서 시각적 경관 요소와 더불어 경관자체의 효과를 검증하는 조건부 로짓모델을 제시하는데 그 목적이 있다. 선택적 상황에 따른 경관선호를 측정하기 위하여 지리산 국립공원을 방문한 탐방객에 대하여 영국의 케이른고럼스 국립공원의 경관사진과 지리산 경관사진을 쌍체로 배열한 사진설문조사를 실시하였다. 시각적 경관의 구성요소는 디지털사진측정의 과정을 거쳐 상호 수직적인 주요인들로 축약되어, 모델의 설명변수로 사용되었다. 연구의 결과, 시각적 경관의 구성요소만으로 형성되는 일반조건부로짓모델은 비유관대안도립성(IIA)의 가정을 충족하지 못하고, 모델의 설명력이 낮게 나타났다(${\rho}^2=0.06$). 반면, 대안특성상수(ASC)로서 표현되는 경관자체의 효과를 포함하는 혼합조건부로짓모델은 비유관대안독립성(IIA)의 가정을 충족하였고, 모델의 적합성 또한 양호하게 나타났다(${\rho}^2=0.25$). 이는 경관선호모델에 있어서 선호의 요인으로써 시각적 경관의 구성요소 뿐만 아니라, 경관자체의 효과를 포함하는 혼합조건부로짓모델이 적합한 것으로 해석된다.

• 생명과학회지
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• 제33권11호
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• pp.887-896
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• 2023

염증반응(inflammation)은 발병, 진행, 악성 전이를 포함한 암의 진행과정(tumorigenesis)에서 중요한 역할을 수행하기 때문에 암 치료를 위한 전략으로 고려되고 있다. 감태(Ecklonia cava) 열수추출물(AEC)의 항암활성 동안 나타나는 항염증 반응을 연구하기 위하여, 비만세포(mast cells)의 분포, inducible nitric oxide synthase (iNOS)단백질, cyclooxygenase-2 (COX-2)단백질, nuclear factor (NF)-κB단백질, inflammasome 구성 단백질, inflammatory cytokines 발현의 변화는 AEC를 5주간 경구투여한 CT26 대장암을 내포하는 BALB/cKorl syngeneic 마우스에서 분석하였다. AEC를 처리한 후, 고형암의 무게와 조직 절편의 괴사 부위가 vehicle처리그룹에 비하여 감소하였다. 비만세포의 수는 vehicle처리그룹에 비하여 AEC처리그룹에서 증가했지만 COX-2와 iNOS의 발현은 AEC처리그룹에서 감소하였다. 또한, NF-κB, NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)과 Caspase-1 (Cas-1)단백질의 발현도 유사한 감소가 관찰되었다. 더불어, tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α)와 interleukin-6 (IL-6)의 mRNA 발현이 vehicle처리그룹에 비하여 AEC처리그룹에서 감소하였다. 이러한 결과는 AEC가 CT26 고형암을 내포하는 BALB/cKorl syngeneic 마우스에서 항암활성은 염증반응과 밀접한 관련이 있음을 제시하고 있다.

• 한국축산식품학회지
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• 제38권5호
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• pp.851-865
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• 2018

This study investigated the prevalence of Listeria monocytogenes in slaughterhouses, and determined serovars and genotypes, and antibiotic resistance of the isolates obtained from slaughterhouses and humans in Korea. Two hundred ninety samples were collected from feces (n=136), carcasses [n=140 (cattle: n=61, swine: n=79)], and washing water (n=14) in nine slaughterhouses. Eleven human isolates were obtained from hospitals and the Korea Center for Disease Control and Prevention. Listeria monocytogenes was enriched and identified, using polymerase chain reaction (PCR) and 16S rRNA sequencing. Serovars and presence of virulence genes were determined, and genetic correlations among the isolates were evaluated by the restriction digest patterns of AscI. Antibiotic resistance of L. monocytogenes isolates were examined against 12 different antibiotics. Of 290 slaughterhouse samples, 15 (5.17%) carcass samples were L. monocytogenes positive. Most L. monocytogenes isolates possessed all the virulence genes, while polymorphisms in the actA gene were found between carcass and human isolates. Serovars 1/2a (33.3%) and 1/2b (46.7%) were the most frequent in carcass isolates. Genetic correlations among the isolates from carcass and clinical isolates were grouped within serotypes, but there were low geographical correlations. Most L. monocytogenes isolates were antibiotic resistant, and some strains showed resistance to more than four antibiotics. These results indicate that L. monocytogenes are isolated from carcass and human in Korea, and they showed high risk serotypes and antibiotic resistance. Therefore, intensive attentions are necessary to be aware for the risk of L. monocytogenes in Korea.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.711-717
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• 2016

Toxoplasma gondii is an obligate intracellular parasite that stimulates production of high levels of proinflammatory cytokines, which are important for innate immunity. NLRs, i.e., nucleotide-binding oligomerization domain (NOD)-like receptors, play a crucial role as innate immune sensors and form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of $pro-IL-1{\beta}$. To elucidate the role of inflammasome components in T. gondiiinfected THP-1 macrophages, we examined inflammasome-related gene expression and mechanisms of inflammasome-regulated cytokine $IL-1{\beta}$ secretion. The results revealed a significant upregulation of $IL-1{\beta}$ after T. gondii infection. T. gondii infection also upregulated the expression of inflammasome sensors, including NLRP1, NLRP3, NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and NAIP, in a time-dependent manner. The infection also upregulated inflammasome adaptor protein ASC and caspase-1 mRNA levels. From this study, we newly found that T. gondii infection regulates NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and neuronal apoptosis inhibitor protein (NAIP) gene expressions in THP-1 macrophages and that the role of the inflammasome-related genes may be critical for mediating the innate immune responses to T. gondii infection.

• 한국공간정보시스템학회 논문지
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• 제3권1호
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• pp.75-87
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• 2001

컴포넌트 소프트웨어의 가장 큰 장점은 응용 소프트웨어사이의 컴포넌트 재사용이다. 본 논문에서는 컴포넌트 재사용의 장점을 지리정보시스템 분야에 접목하여 지방자치단체에서 지리정보시스템을 빠르고 효과적으로 구축하기 위한 컴포넌트의 설계와 구현 방법을 설명하였다. 구현된 컴포넌트는 응용 서비스 컴포넌트로 부산시에서 1999년도에 수행한 사업과 1999년과 2000년에 개방형 GIS 컴포넌트 S/W 개발 과제의 결과물을 기반으로 지방자치단체에서 수행하는 서로 다른 업무 사이에 공통적으로 적용 가능한 기능을 도출하여 구현하였다. 분석 대상이 된 업무는 지방자치단체에서 지리정보시스템으로 구축하는 빈도가 높은 상수도관리 업무, 하수도 관리 업무, 도로 관리 업무, 도시 계획 업무, 지적 관리 업무이다. 분석 과정을 통하여 5개의 업무들 사이에 11개의 공통 기능을 추출하였으며 이 중 7개의 기능을 5개의 응용 서비스 컴포넌트로 구현하였다. 구현된 컴포넌트는 Authority, GISReport, MapService, SymbolManager, AttributeDisplay등 5개로써 사용자 관리, 측정, 검색, 출력, 인덱스 창, 심볼 관리, 속성 정보 표현, 출력 등의 기능을 수행한다.

• IMMUNE NETWORK
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• 제20권5호
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• pp.39.1-39.13
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• 2020

Sjögren's syndrome (SS) is a chronic and systemic autoimmune disease characterized by lymphocytic infiltration in the exocrine glands. In SS, type I IFN has a pathogenic role, and recently, inflammasome activation has been observed in both immune and non-immune cells. However, the relationship between type I IFN and inflammasome-associated pyroptosis in SS has not been studied. We measured IL-18, caspase-1, and IFN-stimulated gene 15 (ISG15) in saliva and serum, and compared whether the expression levels of inflammasome and pyroptosis components, including absent in melanoma 2 (AIM2), NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein (ASC), caspase-1, gasdermin D (GSDMD), and gasdermin E (GSDME), in minor salivary gland (MSG) are related to the expression levels of type I IFN signature genes. Expression of type I IFN signature genes was correlated with mRNA levels of caspase-1 and GSDMD in MSG. In confocal analysis, the expression of caspase-1 and GSDMD was higher in salivary gland epithelial cells (SGECs) from SS patients. In the type I IFN-treated human salivary gland epithelial cell line, the expression of caspase-1 and GSDMD was increased, and pyroptosis was accelerated in a caspase-dependent manner upon inflammasome activation. In conclusion, we demonstrate that type I IFN may contribute to inflammasome-associated pyroptosis of the SGECs of SS patients, suggesting another pathogenic role of type I IFN in SS in terms of target tissue -SGECs destruction.

• 한국안전학회지
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• 제32권6호
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• pp.61-67
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• 2017

Suspended sediment transport plays principal roles in morphological process of natural coastals. It is needed to understand the reason why interaction characteristics of solitary wave and suspended sediment. The present study shows that suspended sediment pickup derived on solitary wave celerity. The 2D prismatic open channel length is 12 m, width is 0.8 m, height is 0.75 m and slope is 1/6. Generation of solitary wave is used by rapidly opening the sluice gate. Bottom surface sediments are laid movable slope section by 0.03 m thickness and experimental sediments are used anathracite and jumoonjin sand. Techniques of suspended sediment pickup rate are designed equipment ASC(Absorptive Suspended sediment Collector). It could directly absorb 5 points suspended sediment by channel water depth. Solitary wave celerity is measued by ADV(Acoustic Doppler Velocimeter). Mounted two video cameras(Model No. : Sony, HDR-XR550) are used to image processing of suspended sediment concentration and turbidity. Suspended sediment pikcup rate(Einstein, 1950) is analyzed to nondimensionalization based on solitary wave celerity. The suspended sediment pickup rate is suggested that more effective plunging breaking type than spilling. The results indicates fundamental suspended sediment transport mechanism between solitary wave celerity and suspended sediment pickup based on laboratory experiments. Finally, the present study suggests that suspended sediment pickup rate by solitary wave is used only characteristics of sediment and solitary wave celerity.

• The Korean Journal of Physiology and Pharmacology
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• 제1권2호
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• pp.185-193
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• 1997

The characteristics of $Na^+$-dependent cycloleucine uptake was investigated in OK cells with regard to substrate specificity and regulation by protein kinase C (PKC). Inhibition studies with different synthetic and natural amino acids showed a broad spectrum affinity to neutral amino acids regardless of their different side chains including branched or aromatic, indicating that the $Na^+$-dependent cycloleucine uptake in OK cells is mediated by System $B^o$ or System $B^o$-like transporter rather than the classical System A or ASC. Phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate, but not $4{\alpha}-PMA$ elicited a time-dependent biphasic stimulation of $Na^+$-dependent cycloleucine uptake, which produced early transient peak at 30 min and late sustained peak at 180min. Both the early and late stimulations by PMA were due to an increase in Vmax and not due to a change in Km. PKC inhibitors blocked both the early and late stimulation by PMA, while protein synthesis inhibitors blocked the late stimulation only. These results suggest the existence and regulation by PKC of System $B^o$ or System $B^o$-like broad spectrum transport system for neutral amino acids in OK cells.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.1289-1293
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• 2015

Background: Anal intraepithelial lesions (AIL) are likely to represent a precursor for anal cancer. Women infected with human immunodeficiency virus (HIV) may be at higher risk of anal cancer but a screening program for AIL still is not routinely recommended. We here studied the relationship of dysplastic cells from cervical and anal cytology in HIV-infected women. Materials and Methods: This prospective study was conducted in Prapokklao Hospital, Thailand during 2013-2014. Five hundred and ninety nine HIV-infected women were recruited. Participants who had cytological reports of equally or over "abnormal squamous/glandular cells of undetermined significance" (ASC-US) were classified as abnormal cervical or anal cytology. Descriptive statistics and logistic regression analysis were used to evaluate correlations between groups. Results: HIV-infected women with abnormal cervical cytology had 3.8 times more risk (adjusted odd ratio 3.846, 95% confidence interval 1.247-11.862, p-value. 019) for abnormal anal cytology. The major problem of the anal Pap test in this study was the inadequacy of the collected specimens for evaluation (34.4%, 206/599). Sensitivity, specificity, positive predictive value, negative predictive value and accuracy of cervical and anal Pap tests were 93.9/12.0, 87.3/96.9, 39.7/21.4, 99.4/94.1 and 88.1/91.4 percent, respectively. Conclusions: Abnormal cervical cytology in HIV-infected women indicates elevated risk for abnormal anal cytology. The sensitivity of the anal Pap test for detection of AIL 2/3 in HIV-infected women was quite low while specificity was excellent. Inadequacy of specimen collection for evaluation was a major limitation. Improvement of sample collection is recommended for future investigations.