• 한국식품과학회지
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• 제27권6호
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• pp.958-963
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• 1995

키조개의 부산물을 향미제로 개발하기 위하여 반응표면분석법으로 가수분해조건을 디자인하여 키조개 부산물의 가수분해물 제조를 시도한 결과, 상업용 단백질 분해효소 11종 가운데 효소활성을 판매가격에 대한 비율로 환산했을 때 APL 440이 가장 경제성이 있었다. 그리고 키조개 부산물 가수분해과정 중 자가소화 효소에 의한 영향은 무시할 정도로 적었다. 반응표면분석결과 얻어진 가수분해율(%DH)은 $%DH=51.126+2.419pH+2.415T-2.426S-2.846pH^2-4.211T^2-3.014t^2+2.419S2$였다. 그러나 정상점이 안장점을 나타내am로 능선분석(반경 0.5) 결과 최대점은 pH 10.2, 온도 $61.4^{\circ}C$, 기질농도 30.9%, 기질에 대한 효소농도 0.32%에서 2.58시간 가수분해할 때이며, 실제 이 조건에서 61.80%의 가수분해율을 보였다. 분말화한 가수분해물은 아미노질소 및 염도가생시료에 비하여 각각 3.5배 및 7.7배 증가하였다.

• 한국경제지리학회지
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• 제15권1호
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• pp.77-94
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• 2012

본 연구의 목적은 우리나라 지역 산업의 수직적 생산 분화를 분석하는 데 있다. 본 연구에서는 2005년 16개 지역 간 산업연관표를 대상으로 현시비교우위지수를 적용하여 지역 산업의 이출경쟁 및 이입대체 능력을 분석하였고 평균전파길이를 추정하여 산업의 분화 정도를 평가하였다. 그 결과로 우선, 경남지역에서는 전기가스수도업에 대해, 인천, 광주 지역에서는 소비제조업에 대해 이출 경쟁력 및 이입 대체 경쟁우위가 나타났다. 또한 평균전파길이는 해당 지역이 공간적, 사회경제적으로 인접할수록, 간접 생산유발효과의 비중이 클수록 높게 나타났다. 산업기반이 발달한 지역일수록, 타 산업에 중간재를 공급하는 기능이 강할수록 생산 사슬 내에서 전방에 위치하였다. 마지막으로 대규모 공단 입지 지역의 제조업 및 서울 소재 서비스업은 전, 후방 평균전파길이가 모두 길게 나타나 이들 산업의 분화 정도가 높으며 산업군의 동반성장을 유도하는 선도 산업의 성격을 갖는다고 볼 수 있다.

• Journal of Ginseng Research
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• 제45권2호
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• pp.295-304
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• 2021

Background: Acute promyelocytic leukemia (APL) is a hematopoietic malignancy driven by promyelocytic leukemia-retinoic acid receptor A (PML-RARA) fusion gene. The therapeutic drugs currently used to treat APL have adverse effects. 20(S)-ginsenoside Rh2 (GRh2) is an anticancer medicine with high effectiveness and low toxicity. However, the underlying anticancer mechanisms of GRh2-induced PML-RARA degradation and apoptosis in human APL cell line (NB4 cells) remain unclear. Methods: Apoptosis-related indicators and PML-RARA expression were determined to investigate the effect of GRh2 on NB4 cells. Z-VAD-FMK, LY294002, and C 87, as inhibitors of caspase, and the phosphatidylinositol 3-kinase (PI3K) and tumor necrosis factor-α (TNF-α) pathways were used to clarify the relationship between GRh2-induced apoptosis and PML-RARA degradation. Results: GRh2 dose- and time-dependently decreased NB4 cell viability. GRh2-induced apoptosis, cell cycle arrest, and caspase3, caspase8, and caspase9 activation in NB4 cells after a 12-hour treatment. GRh2-induced apoptosis in NB4 cells was accompanied by massive production of reactive oxygen species, mitochondrial damage and upregulated Bax/Bcl-2 expression. GRh2 also induced PML/PML-RARA degradation, PML nuclear bodies formation, and activation of the downstream p53 pathway in NB4 cells. Z-VAD-FMK inhibited caspase activation and significantly reversed GRh2-induced apoptosis and PML-RARA degradation. GRh2 also upregulated TNF-α expression and inhibited Akt phosphorylation. LY294002, an inhibitor of the PI3K pathway, enhanced the antitumor effects of GRh2, and C 87, an inhibitor of the TNF-α pathway, reversed NB4 cell viability, and GRh2-mediated apoptosis in a caspase-8-dependent manner. Conclusion: GRh2 induced caspase-dependent PML-RARA degradation and apoptosis in NB4 cells via the Akt/Bax/caspase9 and TNF-α/caspase8 pathways.

• 대한산업공학회지
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• 제14권1호
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• pp.51-56
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• 1988

A spectrum analysis method is presented with an example as an aid to Box and Jerkins' model identification procedure, where the theoretical spectrum of ARMA model and its confidence intervals derived by chi-square distribution are compared. An APL (A Programming Language) program for the method is developed for the 16-bit personal computer.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2002년도 춘계학술대회 논문집
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• pp.352-356
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• 2002

In this paper, an automated design system of suspension and steering parts is developed. The system automates the processes of 3-D modeling and 2-D drafting of the parts. In addition, the BOM and dimension data of the designed part is also automatically transferred to the database of ERP system. The system is developed by using the functions of parametric design and API(application Programming Interface) of the a commercial solid modeler.

• 한국국방경영분석학회지
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• 제13권1호
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• pp.65-90
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• 1987

This thesis describes the curve fitting algorithm that the Portable Wear Metal Analyzer used for calculating concentrations in ppm and compares this with some alternative algorithms. Each algorithm considered fits a curve to the three standards used, which were $20\%,\;50\%,\;and\;100\%$ of the full scale for all nine wear metals. APL was used for all programs.

• 산업경영시스템학회지
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• 제21권45호
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• pp.93-100
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• 1998

The researches of Linear Programming are Khachiyan Method, which uses Ellipsoid Method, and Karmarkar, Affine, Path-Following and Interior Point Method which have Polynomial-Time complexity. In this study, Karmarkar Method is more quickly solved as 50 times then Simplex Method for optimal solution. but some special problem is not solved by Karmarkar Method. As a result, the algorithm by APL Language is proved time efficiency and optimal solution in the Primal-Dual interior point algorithm. Furthermore Karmarkar Method and Primal-Dual interior point Method is compared in some examples.

• 대한약침학회지
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• 제5권1호
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• pp.171-180
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• 2002

Objective: The purpose of this study is to develop and activate the methods of using the arsenic compounds as the anti-cancer medicine. Methods: We investigated some literatures on the using methods, the effects for anti-cancer, and the toxicity of the arsenic compounds. Results: The results are summarized as follows. 1. As is the one of the nitrogen familIy(5A familly). 2. The Arsenic compounds which have been used as the one of the oriental medicine are the Arsenicum Sulbimatum($As_2O_3$) and the Realgar(AsS). 3. As+ 3 is more toxic than the other arsenic compounds. The fatal amount is 100-300 mg. So, it is used 1-5 mg/day as a medicine. 4. The Arsenicwn Sulbimatum($As_2O_3$) and the Realgar(AsS) are used after the heat treatment or the boiling with the acetic acid. 5. The gastrointestinal tract, vessel, and respiration are affected by the acute toxicity of the arsenic compounds. 6. The arsenic compounds are good for the dermatosis and the malignant cancer, especially the acute promyelocytic leukcrnia(APL). we should study the reason of these and the different effect in concentration, also develop new methods of using the the arsenic compounds as getting rid of their toxicity.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.119-124
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• 2003

This study was conducted to evaluate the practical usefulness of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PACE) fingerprinting of whole cell proteins far the identification of lactic acid bacteria in Kimchi. SDS- PACE of whole cell proteins of the reference strains and lactic acid bacteria isolated from Kimchi yielded differential banding patterns that were highly specific fingerprints, thus making it possible to identify. Identification of the isolates from Kimchi was achieved by comparing the SDS-PAGE fingerprints of isolates to those of reference strains. In addition, the reliability of SDS-PAGE was examined by comparing the results with those of the APL 50 CHL system assay and 16S rRNA gene sequence. SDS-PACE assay showed a different identity to reference strains, while the APL 50 CHL system and 16S rRNA gene sequence could not distinguish a few strains. Therefore, SDS-PAGE of the whole cell proteins is a specific and a reliable method that will be useful for the identification of lactic acid bacteria in Kimchi to the species level, and can be used as an alternative or complementary identification method.

• 한국콘텐츠학회논문지
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• 제14권6호
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• pp.241-246
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• 2014

급성전골수구성 백혈병(Acute promyelocytic leukemia, APL)은 혈액암으로 치료가 쉽지 않을 뿐만 아니라 항암치료에 가장 효과가 좋다는 방사선 치료를 해도 오히려 정상세포에도 작용하여 부작용을 초래하게 된다. 본 연구에서는 전자선(EB)을 TNF-${\alpha}$와 같이 처리하였을 경우 정상세포와 암세포의 세포 죽음에 어떠한 영향을 미치는지 확인하였다. HL-60세포는 APL세포주로서 사용하였고 DMSO를 처리하여 분화시킨 HL-60세포는 정상과립구의 성질을 나타내어 정상대조군으로 이용하였다. 그 결과 TNF-${\alpha}$를 전자선을 처리한 HL-60세포에서만 세포독성효과를 나타내었고 이 과정에서 TNF-${\alpha}$는 caspase3를 활성화 시켜서 세포자멸사를 유도하여 세포가 죽음에 이르게 하였다. 결론적으로 TNF-${\alpha}$는 항암치료의 부작용을 없애기 위해 저농도의 전자선 치료 시 함께 사용하여 암 세포의 제거를 증가시켜 암의 치료효율을 높일 수 있는 유효물질로 사료된다.