• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2009년도 추계학술대회 논문집
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• pp.393-394
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• 2009

• KSBB Journal
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• 제24권3호
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• pp.279-286
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• 2009

방선균은 다양한 생리활성 물질을 이차대사산물로 생산하는 산업적으로 매우 유용한 미생물이다. 이에 따라 많은 연구진들이 방선균에 대한 분자생물학적 연구와 산업적 이용에 대한 연구들을 수행하고 있다. 방선균 중에서도 S. avermitilis는 강력한 구충효과가 있는 avermectin을 생산하지만, 또한 포유동물 세포의 미토콘드리아에서 산화적 인산화반응을 억제하는 oligomycin도 함께 생성된다. 따라서 S. avermitilis에서 oligomycin의 생성을 제거시키기 위하여 oligomycin synthetase gene을 disruption 시키기 위한 연구를 수행하였다. 이를 위하여 S. avermitilis로부터 cloning 한 oligomycin synthetase gene (olmA5)의 중앙부분에 apramycin resistance gene을 끼워 넣어 integration vector로 구축한 후에 S. avermitilis의 chromosomal DNA와의 homologous recombination에 의하여 olmA5 gene의 disruption을 유도하였다. Disruption mutants (olmA5::apra)는 PCR을 통해 olmA5 gene의 위치에 apramycin resistance gene이 존재하는 것으로 확인하였고, 또한 HPLC 분석을 통해 oligomycin 생합성이 완전히 제거된 것임을 확인하였다. 그러나 disruption mutant (olmA5::apra)를 이용하여 avermectin 만을 생산할 수 있었으나, avermectin의 생산량에는 거의 변화가 없었다. 이러한 mutants는 산업적으로 avermectin을 생산하기 위한 균주 개량의 훌륭한 source가 될 수 있을 것이다.

• 한국기후변화학회지
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• 제9권4호
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• pp.377-384
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• 2018

The chamber method is widely used for measuring methane emission from paddy rice fields. The closed static chamber has advantages of easy installation and removal in the field and low manufacturing cost. However, the manual chamber method requires a lot of labor and has a limited sampling time and frequency. To overcome the disadvantages of the manual chamber, the auto-chamber system is used for measuring methane emission. We compared the differences in methane flux between the auto-chamber and manual chamber. To investigate methane emissions by the two methods, a chamber was installed for each of the following treatments : control without rice straw (NA), spring plowing after autumn rice straw application (SPRA) and autumn plowing after autumn rice straw application (APRA). The total methane emission was lowest in the control and highest in APRA with both methods. There was no significant difference in total methane emission between the methods, but dynamic fluctuation in methane with temperature change was accurately measured in the auto-chamber. Measuring methane emission with an auto-chamber system is expected to reduce uncertainty and increase accuracy, accompanied by labor reduction.

• 한국자동차공학회논문집
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• 제19권1호
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• pp.38-44
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• 2011

Remanufacturing is restoring or manufacturing the worn-out or discarded components of an end of life product in order to bring it to the "like new" condition. The aim is to reprocess used products in such a manner that the quality of the products is as good as or better than the new one, in terms of appearance, reliability and performance. This paper investigates the automotive remanufacturing industry in Europe. To further knowledge in this field, the paper focuses on the remanufacturing of the automotive components of end-of-life vehicles. The paper scope emphasizes key remanufacturing companies, which are identified and were surveyed in terms of their business structures. The research aims to address the potential for growth within the remanufacturing industry, with regard to various players. The state of the art in remanufacturing of automotive equipment will be identified.

• 한국정밀공학회지
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• 제27권3호
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• pp.58-65
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• 2010

Remanufacturing is a process that restores old products to perform like new, while saving energy, reducing consumption of natural resources, and lowering environmental emissions. By extending the product life cycle, remanufacturing approaches enable closed loop material cycles that are ultimately necessary for a sustainable society. This paper provides some description of the current automotive remanufacturing enterprise, with a particular emphasis on key vehicle components that are currently remanufactured. The analysis yields two major conclusions. First, volume of the USA automotive after sales and remanufacturing industry market is estimated. Second, market price of a remanufactured component in the automotive sector is surprisingly uncorrelated with the number of companies engaged in remanufacturing that component - at least for companies registered with the Automotive Parts Remanufacturing Association (ARPA).