• Journal of Acupuncture Research
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• v.20 no.1
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• pp.61-73
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• 2003

Objective: The purpose of this study is to compare Sa-Am's Ohaeng-acupuncture(舍巖五行鍼法) with Linqi-aupuncture(六氣鍼法)-the transforms of Sa-Am's Ohaeng-acupuncture. Methods : Zheng Ge(正格), Sheng(勝格), Han Ge(寒格), Re Ge(熱格) of Sa-Am's Ohaeng-acupuncture compared with Liuqi-acupuncture(六氣鍼法)-therapy form invigoration and purgation of five zang-fu's wind. heat, dapness, dryness and cold(風熱濕燥寒). Results: 1. Liuqi-acupuncture used five-su points(五兪穴) and Zi-Ta Jing Bu Xie(自他經補瀉). 2. Liuqi-acupuncture is reinforced or reduced itself-point of itself-meridian(自經自穴) in therapy for invigoration and purgation. 3. Liuqi-acupuncture is therapy for invigoration and purgation of five zang-fu's wind, hear, dampness, dryness and cold(風熱濕燥寒). 4. Zheng Ge is similar to Bu-fa, Sheng Ge is similar to Xie-fa in Qu-xue of Ta-jing. The Qu-xue of interrestraining relations is the same, but that of interdependent relation is the difference in Qu-xure of Zi-jing. 5. Han Ge and Re Ge is similar to Re Bu Xie fa in Qu-xue fo Zi-jing but is different to in Ta-zing. For example, Han Ge is Shaofu(Bu), Yingu(Xie) but Re Ge is Shaofu(Xie), Yingu(Bu).

• Journal of Pharmaceutical Investigation
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• v.36 no.5
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• pp.309-314
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• 2006

The multicellular layers(MCL) of human cancer cells is a three dimensional(3D) in vitro model for human solid tumors which has been used primarily for the assessment of avascular penetration of anti-cancer drugs. For anti-cancer drugs with penetration problem, MCL represents a good experimental model that can provide clinically relevant data. Calcein-AM is a fluorescent dye that demonstrates the cellular vitality in a graded manner in cancer cell culture system. In the present study, we evaluated the use of calcein-AM for determination of anti-proliferative activity of anti-cancer agents in MCL model of DLD-1 human colorectal cancer cells. Optical sectioning of confocal imaging was compromised with photonic attenuation and penetration barrier in the deep layers of MCL. By contrast, fluorescent measurement on the cryo-sections provided a feasible alternative. Cold pre-incubation did not enhance the calcein-AM distribution to a significant degree in MCL of DLD-1 cells. However, the simultaneous determination of drug penetration and cellular vitality appeared to be possible in drug treated MCL. In conclusion, these data suggest that calcein-AM can be used for the simultaneous determination of drug-induced anti-proliferative effect and drug penetration in MCL model.

• Korean Journal of Construction Engineering and Management
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• v.23 no.1
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• pp.106-112
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• 2022

Additive manufacturing (AM, also known as 3D printing) technology gets attention for various effects in the construction industry. It reveals abilities of process automation, high traceability of resource management, construction period precision improvement, and worker safety. However, unlike the existing construction technology, the development of AM construction process causes trial errors and unpredictable accidents. In the present study, the construction AM process is designed for on-site construction, and it performs with empirical tests. Also, we analyzed the causes of qualitative experimental results.

• Journal of Acupuncture Research
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• v.17 no.1
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• pp.129-142
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• 2000

Gamdutang aqua-acupuncture solution(GAS), Gamdutang water-extracted solution(GWS) and Degamdutang aqua-acupuncture solution(DGAS) were prepared and tested for potential antitumor activities. It was used three biomarkers (quinone reductase, omithine decarboxylase, glutathione) to test chemopreventive potentials of GAS, GWS, DGAS. GAS was potent inducer of quinone reductase activity in Hepalclc7 murine hepatoma cells in culture, whereas GWS is less potent. GAS, GWS and DGAS were significantly induced quinone reductase activity in cultured rat normal liver cell, Ac2F. Glutathione levels were increased about 1.8-fold with GAS, 1.0-1.1 fold with GWS, DGAS in cultured murine hepatoma hepaiclc7 cells. In addition glutathione s-transferase levels were increased with GAS, GWS and DGAS. The effects of GAS, GWS and DGAS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba castellanii was inhibited by GAS, GWS and DGAS at concentradons of $1{\times}$ and $5{\times}$. These results suggest that GAS has chemopreventive potential by inducing quinone reductase and quinone reductase activities, inhibition of ornithine decarboxylase activity, and increasing glutathione levels.

• Journal of Korean Neurosurgical Society
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• v.30 no.9
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• pp.1103-1107
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• 2001

Objective : Atypical and malignant meningiomas(AM, MM) are known to be rare and show aggressive behavior. Limited data are available concerning the clinical features, effectiveness of surgical removal and role of radiation therapy with AM, MM. The authors report our experience of AM, MM, with respect to clinical features. Methods : Twenty-four cases of AM and 28 cases of MM, who were operated between 1988 and 1999 were retro-spectively studied review of medical records and radiological findings. These were compared with control group of 24 cases of menigiomas manifestating usual clinical course, which are selected arbitrarily among operative cases between Apr. 1999 and Dec. 1999. Mean follow-up periods were 26(1-91) months for AM and 23(1-62) for MM. Authors analyzed the prognostic factors for survival, and statistical analysis were accomplished by Kaplan-Meier method and log-rank test. Results : Differences of clinical features between control groups and these atypical and malignant meningioma group were not significant. However, the location of MM was frequent in non-basal area(p<0.01). In AM, there were 4 patients of recurrence, and 3 patients of mortality. Among mortality cases, only one patient died of tumor progression, the other patients died of other causes. The survival at 2 year and 5 year in this group were 88% and 74% respectively, and in MM, 11 patients died due to tumor progression and 2 had spinal metastasis. The survival at 2 year and 5 year were 72% and 20%, respectively. For extent of resection, total removal(Simpson grade 1 or 2) was less often achieved in MM compared with AM(50% vs. 83%). Extent of resection of tumor and postoperative radiation therapy did not affect survival in both AM, MM. Conclusions : Clinical behavior of AM showed more benign than that of MM. Prognostic factor for survival is not related extent of resection of tumor and postoperative radiation therapy. However, further investigation with long-term follow-up and additional cases is mandatory.

• Safety and Health at Work
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• v.10 no.2
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• pp.229-236
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• 2019

Background: Emerging reports suggest the potential for adverse health effects from exposure to emissions from some additive manufacturing (AM) processes. There is a paucity of real-world data on emissions from AM machines in industrial workplaces and personal exposures among AM operators. Methods: Airborne particle and organic chemical emissions and personal exposures were characterized using real-time and time-integrated sampling techniques in four manufacturing facilities using industrial-scale material extrusion and material jetting AM processes. Results: Using a condensation nuclei counter, number-based particle emission rates (ERs) (number/min) from material extrusion AM machines ranged from $4.1{\times}10^{10}$ (Ultem filament) to $2.2{\times}10^{11}$ [acrylonitrile butadiene styrene and polycarbonate filaments). For these same machines, total volatile organic compound ERs (${\mu}g/min$) ranged from $1.9{\times}10^4$ (acrylonitrile butadiene styrene and polycarbonate) to $9.4{\times}10^4$ (Ultem). For the material jetting machines, the number-based particle ER was higher when the lid was open ($2.3{\times}10^{10}number/min$) than when the lid was closed ($1.5-5.5{\times}10^9number/min$); total volatile organic compound ERs were similar regardless of the lid position. Low levels of acetone, benzene, toluene, and m,p-xylene were common to both AM processes. Carbonyl compounds were detected; however, none were specifically attributed to the AM processes. Personal exposures to metals (aluminum and iron) and eight volatile organic compounds were all below National Institute for Occupational Safety and Health (NIOSH)-recommended exposure levels. Conclusion: Industrial-scale AM machines using thermoplastics and resins released particles and organic vapors into workplace air. More research is needed to understand factors influencing real-world industrial-scale AM process emissions and exposures.

• Journal of Korea Foundry Society
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• v.29 no.3
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• pp.144-149
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• 2009

In this study, we investigated optimum condition of cooling plate method to obtain semi-solid AM100A Mg alloy with fine and globular morphology. AM100A Mg alloy were hot extruded at $380^{\circ}C$ extrusion temperature under extrusion ratio of 25 : 1 and ram speed of 2.4 mm/sec. Vickers hardness test, optical microscopy, scanning electron microscopy, and image analyzer were performed to identify the optimum conditions of cooling plate method. Optimum conditions of cooling plate method to fabricate semi-solid AM100A Mg alloy with fine and globular microstructures were achieved at a pouring temperature of $602^{\circ}C$ and the angle of cooling plate of 60 degree.

• The Korean Journal of Food And Nutrition
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• v.18 no.1
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• pp.72-80
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• 2005

To prevent and treat the osteoporosis, diverse therapies have been applied, which is still need to solve negative side effects. We investigated the effects of the extract from alternative medicine(AM) on the progress of bone loss in ovariectomized-rats fed with Ca-deficient diet for 7 or 14 weeks. Ovariectomy(OVX) concomitant with Ca-deficiency caused bone loss evidently decreased in bone mineral density and bone strength of femoral epiphysis and vertebrae, which were ameliorated with administration of AM extract. Also, the effect of AM extract on the biochemical markers were measured. The increased serum alkaline phosphatase caused by OVX and Ca-deficiency were observed, which were not affected by administration of AM extract. Administration of AM extract may have preventive effect on the elevated serum acid phosphatase concentrations caused by OVX and Ca-deficiency at 14 wks, implicating that AM extract possibly acts toward reducing born resorption, even though the results were not statistically significant. Serum osteocalcin and urinary deoxypyridinoline, the markers of bone turn over, were not changed by estrogen deficiency or AM extract. We concluded that the AM extract treatment had potently preventive effects on the decreased bone density and bone strength induced by OVX and Ca-deficiency. The changes of biochemical markers related to the effect of AM extract were not manifested but it still suggest that AM extract may inhibit the bone resorption derived from OVX and Ca-deficiency.

• YAKHAK HOEJI
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• v.52 no.6
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• pp.419-425
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• 2008

A qualitative and quantitative analytical method was developed for detection of methamphetamine (MA) and its main metabolite amphetamine (AM) in oral fluid. Oral fluids of eleven drug abusers were provided by Police, specimens were collected by stimulation with a cotton swab treated with 20 mg of citric acid ($Salivette^{(R)}$; Sarstedt, USA). As the preliminary test, oral fluid samples were screened for amphetamines by Fluorescence Polarization Immunoassay (TDxFLx, Abbott Co.). Extraction for MA was performed using solid-phase extraction (SPE) by $RapidTrace^{TM}$ (Zymark, USA) with mixed mode cation exchange cartridge, CLEAN $SCREEN^{(R)}$ (130 mg/3 ml, UCT) after dilution with phosphate buffer. Samples were evaporated and derivatized by pentafluoropropionic acid anhydride (PFPA). Quantitation of MA and AM was performed by gas chromatography-mass spectrometry (GC-MS) using selective ion monitoring (SIM), the quantitation ions were m/z 204 (MA), 208 (MA-$D_5$), 190 (AM) and 194 (AM-$D_5$). The selectivity, linearity of calibration, limit of detection (LOD) and quantification (LOQ) within- and between day precision, accuracy and recoveries were examined as parts of the method validation. All oral fluid samples gave positive results to immunoassay for MA (cut-off level, 50 ng/ml as d-amphetamine). Concentrations of MA and AM by GC-MS in eleven samples were ranged 104.2${\sim}$4603.3 ng/ml and 32.4${\sim}$268.6 ng/ml, respectively. Extracted calibration curves of MA and AM were linear over the two concentration range of 1${\sim}$100 and 50${\sim}$1000 ng/ml with correlation coefficient of above 0.999. LOQ of MA and AM was 1 and 3 ng/ml, respectively. The intraand inter-day run precisions (CV) for MA and AM were less than 10%, and the accuracies (bias) for MA and AM were also less than 10% at the two different concentrations 5 and 100 ng/ml at low calibration range, 50 and 1000 ng/ml at high calibration range. The absolute recoveries of MA and AM at low and high calibration ranges were more than 82% and 75%, respectively. In this study the qualitative and quantitative analytical method of MA in oral fluid was established. Oral fluid testing may detect drug use in past hours because of its shorter detection window than urine, and be useful in post-accident situations. So oral fluids will be most useful for testing drug abuse in the driving under the influence of drug (DUID) as the alternative specimens of urine.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.6 no.1
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• pp.25-33
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• 2008

A selective separation of Actirlide(III) by a nPr-BTP/nitrobezene extraction system was studied. The nPr-BTP (2.6-Bis-(5.6-n-propyl-1.2.4-triazin-3-yl)-pyridine) of a environmentally -friendly CHN type was self-synthesized and its compatability with diluent and stability with nitric acid were investigated. At the 0.1M nPr-BTP/nitrobenzene-1M $HNO_3$ and O/A=2, extraction yields of Am used as a representative of Actinide(III) and Eu were about 85% and 8%, respectively, and the other RE elements such as Nd, Ce and Y were extracted less than 3% (separation factor of Am and Eu was about 60). Thus, there was no problems in the selective extraction of Actinide(III) from RE. The stripping yield of Am with 0.05M $HNO_3$ at O/A= 1, however, was about 43% and the maximum stripping yield was 65% at O/A=0.3. It is necessary to develop the stripping system including the stripping agent instead of nitric acid solution.