• Journal of Periodontal and Implant Science
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• 제33권3호
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• pp.359-372
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• 2003

Among objectives of periodontal therapy. the principal one is the morphological and functional reconstruction of lost periodontal supporting tissues. This includes de novo formation of connective tissue attachment and the regrowth of alveolar bone. The use of enamel matrix derivative(EMD) may be a suitable means of regeneration new periodontal attachment in the infrabony defects. Implant used to replace lost tooth but, implantitis occurred after installation. The purpose of this study was to investigate the effects of EMD on differentiation and growth of osteoblast in titanium disc. Twentyfive millimeter diameter and 1mm thick Ti disc which was coated 25, 50, 100, 200${\mu}g$/ml of EMD(Emdogain(R)) used as experimental group, 25, 50, 100, 200ng/d of rhBMP-2 as positive control group, and no coat as negative control group. A human osteosarcoma cell line Saos-2 was cultured in Ti disc and cell proliferation and Alkaline phosphatase (ALP) activity were measured at 1 and 6 days. PCR was performed at 2 and 8 hours. Semi-quantitative RT-PCR for mRNA expressions of various osteoblastic differentiation markers -type I collagen, ALP, osteopontin, and bone sialoprotein - were performed at appropriate concentrations based upon the results of MTT and ALP assay. Cultured cell-disc complexes were prepared for scanning electron microscopy (SEM) at 2 hour. Data were analyzed using Mann-Whitney and repeated- measures 1-way analysis of variance(SPSS software version 10,SPSS. Chicago. IL). After culture, there was more osteoblast in EMD100${\mu}g$/ml than in EMD50, 200${\mu}g$/ml on day 6. There was significant difference in experimental and positive control group compared control group, as times go by(1 and 6 days). Alkaline phosphatase activity was different significantly in EMD100, 200${\mu}g$/ml and BMP100, 200${\mu}g$/ml on day 6. The results of reverse transcriptase-polymerase chain reaction (RT-PCR) showed that expression of mRNA for ALPase, collagen type I, osteopontin. hone sialoprotein and BMP-2 was detected at 2 hour and 8 hour in EMI 200${\mu}g$/ml subgroup and BMP100ng/ml subgroup. The results of this study suggest that application of enamel matrix derivative on osteoblast attached to titanium surface facilitate the expression of bone specific protein and the differentiation and growth of osteoblast.

• 대한치과보철학회지
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• 제46권6호
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• pp.620-627
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• 2008

STATEMENT OF PROBLEM: A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces. PURPOSE: The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/$1{\alpha}$,25-(OH)$_2D_3$ coating with reference to cellular proliferation and differentiation in vitro. MATERIAL AND METHODS: 96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/$1{\alpha}$,25-(OH)$_2D_3$ solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out. RESULTS: PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated $1{\alpha}$,25-(OH)$_2D_3$ (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05). CONCLUSION: Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface.

• 한국식품영양과학회지
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• 제37권4호
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• pp.459-464
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• 2008

본 연구는 고칼슘멸치가 흰쥐의 칼슘대사에 미치는 영향을 조사하기 위해 수행되었다. 실험처리는 시유와 칼슘강화우유, 칼슘강화소재(탄산칼슘, 젖산칼슘) 및 고칼슘대멸 등 5처리군으로 하였고, 대멸분말의 칼슘함량을 고려하여 최종식이의 칼슘함량은 1%가 되도록 semi-purified diet(AINdiet)에 혼합한 식이를 5주 동안 급여하였다. 대퇴골의 길이는 대조군인 우유군(M)와 칼슘강화우유군(M2)이 실험군 중 통계적으로 유의하게 길었고(p<0.05), BMD, BMC 및 칼슘함량은 고칼슘멸치군(MA)이 실험군 중 가장 높았다. 체외(in vitro) 칼슘흡수율은 고칼슘대멸군이 7.30%로 가장 높았고, 생체내(in vivo) 칼슘흡수율은 실험군간 차이는 없었지만 고칼슘대멸군이 27.50%로 가장 높았다. 혈청 중 총 콜레스테롤과 HDL-콜레스테롤 농도는 대조군과 고칼슘대멸군간에 통계적인 차이가 있었다(p<0.05). Creatinine 농도는 대조군, 고칼슘대멸군 및 탄산칼슘군이 칼슘강화우유군에 비해 통계적으로 높았다(p<0.05). 칼슘과 osteocalcin 농도 및 ALP활성은 실험군간에 차이가 없었지만 고칼슘대멸군이 높았다. SGOT활성은 칼슘강화우유군에 비해 대조군, 고칼슘대멸군 및 탄산칼슘군이 유의하게 높았다(p<0.05). 이상의 결과에서 고칼슘대멸군은 칼슘강화소재와 칼슘강화우유에 비해 대퇴골의 BMD, BMC 및 칼슘함량을 증가시켰으며, 생체내외(in vivo, in vitro) 칼슘흡수율에서도 다른 처리군보다 높았다. 본 결과는 대멸을 활용한 고칼슘식품 개발에 유용한 평가 자료로 활용될 것으로 사료된다.