• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.668-680
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• 2020

Bacillus velezensis is an important plant growth-promoting rhizobacterium with immense potential in agriculture development. In the present study, Bacillus velezensis Lle-9 was isolated from the bulbs of Lilium leucanthum. The isolated strain showed antifungal activities against plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea and Fusarium fujikuroi. The highest percentage of growth inhibition i.e., 68.56±2.35% was observed against Fusarium oxysporum followed by 63.12 ± 2.83%, 61.67 ± 3.39% and 55.82 ± 2.76% against Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi, respectively. The ethyl acetate fraction revealed a number of bioactive compounds and several were identified as antimicrobial agents such as diketopiperazines, cyclo-peptides, linear peptides, latrunculin A, 5α-hydroxy-6-ketocholesterol, (R)-S-lactoylglutathione, triamterene, rubiadin, moxifloxacin, 9-hydroxy-5Z,7E,11Z,14Z-eicosatetraenoic acid, D-erythro-C18-Sphingosine, citrinin, and 2-arachidonoyllysophosphatidylcholine. The presence of these antimicrobial compounds in the bacterial culture might have contributed to the antifungal activities of the isolated B. velezensis Lle-9. The strain showed plant growth-promoting traits such as production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, and nitrogen fixation and phosphate solubilization. IAA production was accelerated with application of exogenous tryptophan concentrations in the medium. Further, the lily plants upon inoculation with Lle-9 exhibited improved vegetative growth, more flowering shoots and longer roots than control plants under greenhouse condition. The isolated B. velezensis strain Lle-9 possessed broad-spectrum antifungal activities and multiple plant growth-promoting traits and thus may play an important role in promoting sustainable agriculture. This strain could be developed and applied in field experiments in order to promote plant growth and control disease pathogens.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1484-1489
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• 2014

A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at $10^{\circ}C$ (43% activity remained), with the optimal temperature at $20^{\circ}C$, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.

• Journal of Microbiology and Biotechnology
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• v.29 no.9
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• pp.1470-1477
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• 2019

${\beta}_2$-adrenergic receptor (${\beta}_2-AR$) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of ${\beta}$-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and $59.57{\mu}g/l$, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was $3.2{\mu}g/l$ and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified ${\beta}_2-AR$ would have great application potential in detection of ${\beta}$-agonist residues.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2019.08a
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• pp.292-292
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• 2019

• Journal of Agricultural Extension & Community Development
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• v.13 no.2
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• pp.375-384
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• 2006

농업－공동체 임업은 토지－나무－인간 사이의 관계를 폭넓게 포함하고 있으나 이 논문에서는 농업－공동체 임업활동이 베트남의 산지의 지속가능한 이용에 초점을 두고 있다. 농업－공동체 임업(AF와 CF)은 베트남에서 시급한 국가우선사항이 된 개발과 보전을 함께 고려할 수 있는 기능을 지닌 것으로 받아드려지고 있다. 베트남에서 AF와 CF를 동시에 지원할 수 있는 자원은 한정되어 있으며, 제약 사항은 위협적이고 시급한 상황이고, 황폐화가 진행될수록 복원은 어려워지는데, 농업의 생산성 감소에 따른 산촌주민들의 가족 부양을 위한 생태계 파괴도 가속화되고 있는 것으로 조사되었다. 이 연구는 농업－공동체 임업활동이 베트남의 산지 자원의 지속가능한 이용에 있어서의 연관된 문제들, 발전에의 제한요인, 특히 정책 제한요인을 분석하고 베트남 농업－공동체 임업 개발의 방향을 제시하고자 하였다.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.4
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• pp.169-171
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• 2021

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.223-230
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• 2007

Vitellaria paradoxa commonly called shea is an important agro forestry and fruit-bearing species present in four agro-ecological zones of Cameroon. The goal of this work was the morphological characterization of certain populations of V. paradoxa which will serve as a necessary step for a subsequent genetic study of the species. Morphological observations related to 20 agronomic traits, studied on 8-13 trees selected from each of the eight shea populations across four different agro-ecological zones located in four provinces of Cameroon were studied. The study showed that there was a variation between the populations, related to the traits measured on the trunk, fruit, nut, and leaf. Three shapes of the tree(ball, broom, and trained), five shapes of the fruit(round, oblong, reversed pear, ovoid, and oblong), three colors of the nut(clear brown, dark brown, and blackish brown) were identified. The principal component analysis(PCA) carried out on the quantitative characters revealed 72% of the total variance expressed on the first and second main axis. This variation was essentially explained by the traits measured on the fruits and on the nuts. The analyses showed that only the traits of the fruits and the nuts were discriminative. The shea populations studied were structured into two distinct groups using these discriminative traits.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.4
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• pp.320-326
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• 2016

From the perspective of climate-smart agriculture, it is becoming more critical to accurately estimate the amount of greenhouse gas emissions in the agricultural sector. In order to accurately ascertain the methane emissions from rice paddies, which account for a significant portion of the emission from the agricultural sector, we used the data from the 2010 Agriculture, Forestry and Fisheries Census, the revised water management scaling factors and their calculation program. In order to facilitate the analyses and understanding, the results were mapped using the ArcGIS software. The fact that the validation of the mapped values against the actual field measurements at one site showed little difference encourages the necessity to further this study. The administrative districts-based map of methane emission can help clearly identify the regional differences. Furthermore, the analysis of their major controlling factors will provide important scientific basis for the practical policy makings for methane mitigation.

• Journal of Forest and Environmental Science
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• v.25 no.1
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• pp.43-48
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• 2009

Forests render both a home and a livelihood for people living in and around them. To reconcile the need of local communities with conservation, the Nishorgo Support Project (NSP or Nishorgo) is supporting co-management in five protected areas of Bangladesh, including Rema-Kalenga Wildlife Sanctuary. The main focus of this study is to assess the effectiveness of alternate income generating activities (AIGAs) which is provided by the Nishorgo Support Project. It is a tool for reducing dependence on forest resources by people living in and around Rema-Kalenga Wildlife Sanctuary and also play effective role in forest conservation. This study compares the socioeconomic condition (income, living style etc.) and forest dependency before and after implementation of co-management activities in Rema-Kalenga Wildlife Sanctuary. A total of 11 villages (sampling intensity was 31%) namely Kalibari, Mongoliabari, Chokidarbari, Chonbari, kalenga, Krishnochura, Hatimara, Himalia, Rashidpur, Goramchori and Horinmara were studied. We surveyed 272 households in these villages (Incase of households survey, sampling intensity was 100%). Data analysis shows that the major income generating livelihood activities were agro farming (30%), followed by fuelwood collection (22.50%), nursery raising (12.5%), cattle rearing (10%), fisheries (7.50%) and others. Study also reveals that after being implementation of the co-management activities the average income levels of the studied villages have rises on 578 Tk/households (1US$ = 70 Taka, Taka means Bangladeshi currency). Further more, many of the illegal loggers became forest protector, which make their life more secured. It was also found that peoples of the study villages are now actively engaging in forest management activities and it is the only hope for conservation and restoration of forest resources not only in Rema-Kalenga Wildlife Sanctuary but also in other protected area's of our country.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.12
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• pp.1681-1689
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• 2011

The beneficial effects of tea catechins (TCs) are related not only to their antioxidant potential but also to the improvement of animal meat quality. In this study, we assessed the effects of dietary TC supplementation on plasma biochemical parameters, hormone responses, and glutathione redox status in goats. Forty Liuyang goats were randomly divided into four equal groups (10 animals/group) that were assigned to four experimental diets with TC supplementation at 4 levels (0, 2,000, 3,000 or 4,000 mg TC/kg DM feed). After a 60-day feeding trial, all goats were slaughtered and sampled. Dietary TC treatment had no significant effect on blood biochemical parameters, however, low-density lipoprotein cholesterol (p<0.001), triglyceride (p<0.01), plasma urea nitrogen (p<0.01), and glucose (p<0.001) decreased and total protein (p<0.01) and albumin (p<0.05) increased with the feeding time extension, and day 20 was the turning point for most of changes. Interactions were found in glutathione (p<0.001) and the ratio of reduced and oxidized glutathione (p<0.05) in whole blood between treatment and feeding time. Oxidized glutathione in blood was reduced (p<0.05) by 2,000 mg TC/kg feed supplementation, and a similar result was observed in longissimus dorsi muscle. Though plasma glutathione peroxidase (p<0.01) and glutathione reductase (p<0.05) activities were affected by treatment and feeding time interactions, and glutathione S-transferases activity increased with feeding day extension, no changed values appeared in longissimus dorsi muscle. In conclusion, dietary TC supplementation affected the concentrations of some blood metabolites and accelerated GSH depletion in the blood of goats. In terms of less high-density lipoprotein cholesterol, the highest insulin and IGF-I concentrations, the highest ratio of reduced and oxidized glutathione in plasma, the dosage of 2,000 mg TC/kg feed might be desirable for growing goats to prevent glutathione depletion and keep normal physiological metabolism.