• Title/Summary/Keyword: AGR

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Characterization of a Glycoside Hydrolase Family 50 Thermostable β-agarase AgrA from Marine Bacteria Agarivorans sp. AG17

  • Nikapitiya, Chamilani;Oh, Chul-Hong;Lee, Young-Deuk;Lee, Suk-Kyoung;Whang, Il-Son;Lee, Je-Hee
    • Fisheries and Aquatic Sciences
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    • v.13 no.1
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    • pp.36-48
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    • 2010
  • An agar-degrading Agarivorans sp. AG17 strain was isolated from the red seaweed Grateloupia filicina collected from Jeju Island. A beta-agarase gene from Agarivorans sp. AG17 was cloned and designated as agrA. agrA has a 2,985 bp coding region encoding 995 amino acids and was classified into the glycoside hydrolase family (GHF)-50. Predicted molecular mass of the mature protein was 105 kDa. His-tagged agrA was overexpressed in Escherichia coli and purified as a fusion protein. The enzyme showed 158.8 unit/mg specific activity (optimum temperature at $65^{\circ}C$ and pH 5.5 in acetate buffer) with unique biochemical properties (high thermal and pH stabilities). Enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose by degrading agar, and hydrolyzed neoagaro-oligosaccharides were biologically active. Hence the purified enzyme has potential for use in industrial applications such as the development of cosmetics and pharmaceuticals.

A Study on the Growth Characteristics and Productivity of Different Corn Cultivars at Different Environmental Locations in South Korea

  • Soundharrajan Ilavenil;Jeong Sung Jung;Ki Choon Choi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.3
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    • pp.183-189
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    • 2023
  • It is widely known that forage corn is one of the most important crops in the production of silage and green chops during the summer months. In this study, the characteristics and the productivity of Kwangpyeongok, AGR 41, and Nero IT corn cultivars were evaluated in the regions of Jeju and Cheonan, Korea between 2021 and 2022. In each year, different corn cultivars were sown in different parts of the country on April 7 in Jeju and May 3 in Cheonan. The yield of three cultivars, Kwangpyeongok, AGR 41, and Nero IT were measured at the yellow-ripening stage, including the leaves and stems (Stover) and corn grain. Kwangpyeongok stover and total dry matter (DM) yields were slightly higher in Cheonan than in Jeju over the past two years. The quantity of corn cultivated in all cultivated areas was similar. The average yield of AGR 41, corn quantity and total DM were slightly higher in Jeju than in Cheonan. In terms of stover yield, and total dry matter content of the Nero IT cultivar cultivated in Jeju had a slightly higher yield than the cultivar cultivated in Cheonan. Based on this study, all cultivars can show some variations but not too much in terms of growth productivity and distinct characteristics depending on their location. Overall, AGR 41, and Nero IT cultivars in Jeju exhibit slightly higher levels of productivity than the same cultivars in Cheonan.

Analysis of Geometrical and Physical PRoperties of Red Pepper by Machine Vision (기계시각을 이용한 홍고추의 기하학적 및 물리적 특성 분석)

  • 김영복;이승규;김성태;나우정;송대빈;이호준
    • Journal of Biosystems Engineering
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    • v.26 no.3
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    • pp.287-294
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    • 2001
  • The geometrical and physical properties of red peppers were studied for proper design of a red pepper processor. Mass, volume, roundness and compactness of red peppers were calculated from digital images. They were compared with real data and the relations of them were suggested. Roundness of red peppers was ranged from 0.2 to 0.5 and the average value was 0.349. Compactness of red peppers was ranged from 25 to 50 and the average value was 37.1. The regression equations to calculate the volume and mass of red pepper were obtained as y$\_$v/$\_$=/0.553$\varkappa$$_1$+1.441$\varkappa$$_2$-1.013$\varkappa$$_3$(R=0.95) and y$\_$m/=0.252$\varkappa$$_1$+0.938$\varkappa$$_2$-0.499$\varkappa$$_3$-1.5112 (R=0.93), y$\_$v/:volume(㎤), y$\_$m/:mass(g), $\varkappa$$_1$: perimeter(cm), $\varkappa$$_2$: area(㎠), $\varkappa$$_3$: length of major axis(cm), respectively. The direction for aligning the red pepper in a machine processing was easily and perfectly recognized. The response time for digital image processing has to be reduced for more efficient operation. HSI and YIQ values could be useful for recognizing the red pepper from background.

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The Structure and Processing of the Korean functional category (한국어 기능범주의 정보처리)

  • 황유미;문영선;박혜성;남기춘
    • Proceedings of the Korean Society for Cognitive Science Conference
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    • 2000.05a
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    • pp.273-278
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    • 2000
  • 본 연구는 한국어 기능 범주(functional category)의 처리를 살펴보기 위하여 실문법증 환자를 대상으로 실시되었다. 일반적으로 수형도에서 기능 범주는 보문소구(Complementizer Phrase), 시제구(Tenxe Phrase), 일치소구(Agreement Phrase)의 순으로 구성되어 있다. 본 실험은 Grodzinsky(1997)의 수형도 가지치기 가설과 실문법증의 엄밀성 척도를 바탕으로 한국어 기능 범주의 처리에 있어서도 두 가지 가설의 적용이 타당한지 여부를 검증하기 위해 실시되었다. 실험 1은 일치소(Agr)의 처리를 살펴보기 위하여 실시되었는데, 국어의 존칭 선어말 어미를 중심으로 살펴보았다. 국어의 존칭선어말 어미는 크게 '-시-'삽입에 의한 존칭과 다른 어휘로 대체하는 보충법에 의한 존칭으로 구분된다. '-시-'삽입에 의한 존칭은 AgrP의 범주로 보충법에 의한 존칭은 동사구(Verb Phrase)의 범주로 설정하여 문법에 어려움을 보이는 실문법증의 경우 어휘부에 혹하는 보충법에 의한 존칭을 문법범주에 속하는 '-시-'삽입의 존칭보다 더 잘 할 것으로 가정하였다. 실험 2는 AgrP의 범주인 '-시-'선택의 장애가 TPqja주인 시제 선택에 역시 영향을 미칠 것이라는 가정아래 설계되었다. 수형도 상에서 '-시-'는 일치소(Agr)로서 시제(TP)의 아래에 위치하므로 실험 3은 '-시-'삽입 존칭과 시제에서 어려움이 보문소(CP)의 선택에 역시 영향을 미칠 것이라는 가정하에서 실시되었다. 실험 1의 결과 '-시-'삽입에 의한 존칭을 처리하는데 보충법에 의한 존칭보다 어려움을 보였다. 실험 2의 결과 '-시-'삽입 존칭에 어려움을 보인 환자는 시제 선어말 어미를 선택하는데도 어려움을 보임이 확인되었다. 실험 3 역시 실험 1과 실험2에서와 동일하게 처리의 어려움을 보였다. 이러한 실험 결과들은 국어의 존칭과 시제 선어말 어미가 통사부에서 구(XP)와 결합하여 새로운 구를 형성하는 통사적 접사로 해석할 수 있으며 Grodzinsky의 가설을 지지하는 결과를 보여 줌으로서 국어에서도 AgrP, TP, CP 사이의 통사적 위계가 있음을 뒷받침하는 증거가 된다.

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The Study on the Effect of Density and Moisture Content on Shear Strength of Soils (흙의 밀도(密度)와 함수비(含水比)가 전단강도(剪斷强度)에 미치는 영향(影響))

  • Cho, Seung-Seup;Kang, Sin-Up;Kang, Yea-Mook;Kim, Seung-Wan;Kim, Soung-Rai
    • Korean Journal of Agricultural Science
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    • v.5 no.1
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    • pp.15-28
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    • 1978
  • It has been known that the shear strength of soil is an important design parameter for the foundation of structures, the retaining walls, the slope failures and so forth. In this study, the shear test was performed by using the direct shear apparatus under various degree of the moisture content and the density of the sample soils. The results of the study were summarized as follows; 1. The shear strength of soil increased with increase in the dry density of soil, and at the same level of density of the sample the shear strength of soil showed large values on a good grading of the sample. 2. The cohesion of the soil varied directly with the dry density of it, however the internal friction angle of soil was not affected by the dry density of tile sample. 3. The shear strength of sample varied inversly with the moisture content of it, and this phenomenon was apparent on a good grad ing of sample. 4. The cohesion of soil showed maximum value when the moisture content of the soil reached optimum level and the internal friction angle decreased with increase in the moisture content of it. These phenomena were very obvious on a good grading sample, SDC-1. 5. The cohesion of the soil decreased with increase in void ratio of the sample, but the internal friction angle of the sample didn't show such tendency.

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Sedative Effects of the Essential Oil from Acorus gramineus upon Inhalation

  • Koo, Byung-Soo;Lim, Jae-Chul;Park, Jong-Hee;Lee, Dong-Ung
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.273.2-274
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    • 2003
  • The present study was designed to evaluate central inhibitory effects of an essential oil from Acori graminei Rhizoma (AGR), the dry rhizomes of Acorus gramineus Solander (Araceae) upon fragrance inhalation (aroma therapy). Preinhalation of an essential oil of AGR markedly delayed the appearance of pentylenetetrazole-induced convulsion. Furthermore, the inhalation of an essential oil of AGR impressively inhibited the activity of ${\gamma}$-aminobutyric acid (GABA) transaminase, a degradating enzyme for GABA as inhalation period is lengthened. (omitted)

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Composting Greenhouse using The Forced Aeration Method

  • Hong, Ji-Hyung;Park, Keum-Joo;Sohn, Bo-Kyoon
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.685-691
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    • 1996
  • Recent research in composting greenhouse has focused on some of the fundamental properties during the process such as temperature , carbon dioxide content and odors which change as the composting progresses. The composting greenhouse of cattle manure with rice hulls by a forced aeration method without turning is available for the practical proposition. The control of a predetermined temperature range(45-65$^{\circ}C$) is possible if intermittent aeration is used. The carbon dioxide concentration was maintained in the range from 400 to 2650 ppm by the intermittent aeration. The ammonia emission rose rapidly leading to a temperature increase of composting material up to more than 60$^{\circ}C$ for six days. Ammonia emission declined quickly and could hardly be detected after 10 days of running period.

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Control of Overwintering Striped Rice Borer, Chilo suppressalis W. in Straw Handicrafts with Phostoxin Fumigation (Phostoxin 훈증 처리에 의한 볏짚 제품내의 월동 이화명충 방제)

  • Chang Y. D.;Kim H. S.;Yoo J. K.
    • Korean journal of applied entomology
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    • v.17 no.1 s.34
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    • pp.71-73
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    • 1978
  • Overwintering striped rice borer, Chilo suppressalis W. larvae, pupae and adults in rice straw handicrafts were exposed to different doses and periods of phostoxin in gas chambers(ave.temp. 27C). The following results were obtained; 1. For 100 percent mortality, larvae required 48 hours, pupae 24 hours and adults only 8 hours of exposure. 2. The insecticidal effect of tile fumigant was more dependent upon the length of exposure than the dosage of fumigant.

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Studies on the patho-physiological observation of lower urinary tract disease of cats (고양이 하부뇨로(下部尿路) 질환(疾患)의 병태생리(病態生理)에 관한 연구(硏究))

  • Shin, Sa-kyeng;Sato, Motoyoshi;Miyahara, Kazuro;Hirose, Tsuneo
    • Korean Journal of Veterinary Research
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    • v.36 no.4
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    • pp.967-972
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    • 1996
  • A study was carried on histopathology of LUTD. Based on this study, it was noted that a plural number of factors and some genetic mechanisms are participating in onset of this disease, which should have to be studied further in future in omnidirections.

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Effects of a Slow-Release Fertilizer on Onion (Allium cepa L.) Cultivation in Southwestern Area (서남부 지역 양파 재배 시 완효성 비료 시용효과)

  • Lee, Eul Tai;Cho, Sang Kyun;Song, Yeon Sang;Jang, Young Suk;Choi, In Hu;Oh, Yong Bee
    • Horticultural Science & Technology
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    • v.19 no.4
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    • pp.476-482
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    • 2001
  • This experiment was conducted to evaluate the effect of slow-release fertilizer application on quality elevation of onion (Allium cepa L.) in southwestern sea. The slow-release fertilizers used were NIAST (National Institute of Agricultural Science and Technology) I, II, CDU (Crotonylidene Diurea), UF (Urea-Formaldehydes), and IBDU (Isobutylidene Diurea) 30, 50. Growth of the onion plant treated with slow-release fertilizer showed better than the control. However, a rate of infected plant to downy mildew was low as compared with control. Chlorophyll and total nitrogen contents were high in control, whereas firmness, soluble solid content, number and thickness of scaly leaves were high in slow-release fertilizer plot. In early maturing onion, total marketable bulb yield was higher in slow-release fertilizer plot than all other fertilizer treatments. Total marketable bulb yield in late maturing onion treated with slow-release fertilizer was slightly decreased. Decaying loss and the rate of sprouting during storage period decreased in slow-release fertilizer application. In summary, application of slow-release fertilizer increased marketable yield, storability after harvest and also reduced labor needs for top dressing.

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