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http://dx.doi.org/10.5657/fas.2010.13.1.036

Characterization of a Glycoside Hydrolase Family 50 Thermostable β-agarase AgrA from Marine Bacteria Agarivorans sp. AG17  

Nikapitiya, Chamilani (Department of Marine Life Sciences, Jeju National University)
Oh, Chul-Hong (Korea Ocean Research & Development Institute)
Lee, Young-Deuk (Department of Marine Life Sciences, Jeju National University)
Lee, Suk-Kyoung (Department of Marine Life Sciences, Jeju National University)
Whang, Il-Son (Department of Life Science, Jeju National University)
Lee, Je-Hee (Department of Marine Life Sciences, Jeju National University)
Publication Information
Fisheries and Aquatic Sciences / v.13, no.1, 2010 , pp. 36-48 More about this Journal
Abstract
An agar-degrading Agarivorans sp. AG17 strain was isolated from the red seaweed Grateloupia filicina collected from Jeju Island. A beta-agarase gene from Agarivorans sp. AG17 was cloned and designated as agrA. agrA has a 2,985 bp coding region encoding 995 amino acids and was classified into the glycoside hydrolase family (GHF)-50. Predicted molecular mass of the mature protein was 105 kDa. His-tagged agrA was overexpressed in Escherichia coli and purified as a fusion protein. The enzyme showed 158.8 unit/mg specific activity (optimum temperature at $65^{\circ}C$ and pH 5.5 in acetate buffer) with unique biochemical properties (high thermal and pH stabilities). Enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose by degrading agar, and hydrolyzed neoagaro-oligosaccharides were biologically active. Hence the purified enzyme has potential for use in industrial applications such as the development of cosmetics and pharmaceuticals.
Keywords
Agarivorans sp.; Beta agarase; GHF-50; Neoagaro-oligosaccharides; Thermostable;
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