• Journal of Korean Society on Water Environment
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• v.33 no.3
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• pp.273-281
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• 2017

The objective of this study is to evaluate the performance of stream purification equipment system combined MBP (Micro Bubble Process) and AGBP (Aerobic Granular Biofilm Process). Based on results, the optimal HRT (Hydraulic Retention Time) of MBP and AGBP set as 0.72 and 2.4 h, respectively. In the results of continuous operation on combined MBP and AGBP, it is possible to achieve a BOD, TSS and T-P removal efficiency of 85, 90 and 94%, respectively. It also confirmed that the water quality of the stream can be purified with increasing circulation flow through water simulation evaluation applied the QUAL-NIER model. Consequently this purification system can resolve the stream purification and dry stream problem.

• Journal of Ginseng Research
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• v.48 no.5
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• pp.454-463
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• 2024

Background: Panax quinquefolius L, widely recognized for its valuable contributions to medicine, has aroused considerable attention globally. Different from the extensive research has been dedicated to the root of P. quinquefolius, its berry has received relatively scant focus. Given its promising medicinal properties, this study was focused on the structural characterizations and anti-inflammatory potential of acidic polysaccharides from the P. quinquefolius berry. Materials and methods: P. quinquefolius berry was extracted with hot water, precipitated by alcohol, separated by DEAE-52-cellulose column to give a series of fractions. One of these fractions was further purified via Sephadex G-200 column to give three fractions. Then, the main fraction named as AGBP-A3 was characterized by methylation analysis, NMR spectroscopy, etc. Its anti-inflammatory activity was assessed by RAW 264.7 cell model, zebrafish model and molecular docking. Results: The main chain comprised of α-L-Rhap, α-D-GalAp and β-D-Galp, while the branch consisted mainly of α-L-Araf, β-D-Glcp, α-D-GalAp, β-D-Galp. The RAW264.7 cell assay results showed that the inhibition rates against IL-6 and IL-1β secretion at the concentration of 625 ng/mL were 24.83 %, 11.84 %, while the inhibition rate against IL-10 secretion was 70.17 % at the concentration of 312 ng/mL. In the zebrafish assay, the migrating neutrophils were significantly reduced in number, and their migration to inflammatory tissues was inhibited. Molecular docking predictions correlated well with the results of the anti-inflammatory assay. Conclusion: The present study demonstrated the structure of acidic polysaccharides of P. quinquefolius berry and their effect on inflammation, providing a reference for screening anti-inflammatory drugs.