Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Structural analysis, anti-inflammatory activity of the main water-soluble acidic polysaccharides (AGBP-A3) from Panax quinquefolius L berry

  • Zhihao Zhang (Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences)) ;
  • Huijiao Yan (Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences)) ;
  • Hidayat Hussain (Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry) ;
  • Xiangfeng Chen (Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences)) ;
  • Jeong Hill Park (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Sung Won Kwon (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Lei Xie (Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences)) ;
  • Bowen Zheng (Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences)) ;
  • Xiaohui Xu (Department of Genetics and Cell Biology, Basic Medical College, Qingdao University) ;
  • Daijie Wang (Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences)) ;
  • Jinao Duan (Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine)
  • Received : 2024.01.13
  • Accepted : 2024.05.04
  • Published : 2024.09.01
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Abstract

Background: Panax quinquefolius L, widely recognized for its valuable contributions to medicine, has aroused considerable attention globally. Different from the extensive research has been dedicated to the root of P. quinquefolius, its berry has received relatively scant focus. Given its promising medicinal properties, this study was focused on the structural characterizations and anti-inflammatory potential of acidic polysaccharides from the P. quinquefolius berry. Materials and methods: P. quinquefolius berry was extracted with hot water, precipitated by alcohol, separated by DEAE-52-cellulose column to give a series of fractions. One of these fractions was further purified via Sephadex G-200 column to give three fractions. Then, the main fraction named as AGBP-A3 was characterized by methylation analysis, NMR spectroscopy, etc. Its anti-inflammatory activity was assessed by RAW 264.7 cell model, zebrafish model and molecular docking. Results: The main chain comprised of α-L-Rhap, α-D-GalAp and β-D-Galp, while the branch consisted mainly of α-L-Araf, β-D-Glcp, α-D-GalAp, β-D-Galp. The RAW264.7 cell assay results showed that the inhibition rates against IL-6 and IL-1β secretion at the concentration of 625 ng/mL were 24.83 %, 11.84 %, while the inhibition rate against IL-10 secretion was 70.17 % at the concentration of 312 ng/mL. In the zebrafish assay, the migrating neutrophils were significantly reduced in number, and their migration to inflammatory tissues was inhibited. Molecular docking predictions correlated well with the results of the anti-inflammatory assay. Conclusion: The present study demonstrated the structure of acidic polysaccharides of P. quinquefolius berry and their effect on inflammation, providing a reference for screening anti-inflammatory drugs.

Keywords

Acknowledgement

We gratefully acknowledge the Science, Education and Industry Integration Innovation Pilot Project from Qilu University of Technology (Shandong Academy of Sciences) (2022JBZ02-06), Key R&D Program of Shandong Province (2023TZXD068, 2023TZXD083), the Science and Technology smes Innovation Ability Improvement Project of Shandong Province (2022TSGC2439), the Key R&D and transformation plan of Qinghai Province (2023-SF-112), and the assistance of Yangzhou BoRui Saccharide Biotech Co. Ltd. (www.polyscilife.com) in experiments design and data analysis.

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