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Ingestion size of food microalgae of the Pacific oyster Crassostrea gigas larvae (굴, Crassostrea gigas 유생의 먹이생물 섭취 크기)

  • Hur, Young-Baek;Jeon, Chang-Young;Cho, Kee-Chae;Hur, Sung-Bum
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.307-315
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    • 2011
  • Digestibility index of 12 phytoplankton species were invested during the larval development sizes. Ingestible size of phytoplankton varied depending on larval sizes: Isochrysis galbana, I. aff. galbana, Pavlova lutheri, Chlorella ellipsoidea, Nannochloris oculata was ingested 94.2-99.7% all larval sizes. Cheatoceros calcitrans, C. gracilis and C. simplex could ingest over 90.0% after umbo stage (mean shell length $189.3{\pm}13.8{\mu}m$). Phaeodactylum triconutum, Dunaliella tertiolecta and Tetraselmis tetrathele could not ingested D-shaped larvae (shell length $65.0-100.0{\mu}m$) but ingested 97.3-99.7%, 43.3-99.3%, 48.5-99.3% after then larval stages, respectively. But Thalassiosira weissflogii was ingested 1.0-1.7% only at full grown stage. Over 50.0% ingestion cell size was D-shape stage larvae with smaller than mean $102.3{\mu}m$ in shell length could ingest phytoplankton with $4.6{\mu}m$ in both major and minor axis and up to $9.3{\mu}m$ in minor axis basis for larger than mean $158.3{\mu}m$ in shell length, respectively. At all larval stages, phytoplankton with larger than $10.0{\mu}m$ in both major and minor axis could not be ingested.

New Records of Creeping Ctenophores, Genus Coeloplana (Tentaculata: Platyctenida: Coeloplanidae), from Korea

  • Song, Jun-Im;Hwang, Sung-Jin;Lee, Sang-Hwa;Park, Joong-Ki
    • Animal Systematics, Evolution and Diversity
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    • v.27 no.1
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    • pp.47-52
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    • 2011
  • Creeping ctenophores, Coeloplana species, were collected by SCUBA divers throughout the year (November 2006 to June 2010) from the branches and polyp masses of encrusting dendronephthyas at a depth of 20-32m off Munseom Island (Seogwipo-si, Jeju-do, Korea). A single individual of a newly recorded species in Korea, Coeloplana bocki Komai, 1920, was collected together with C. anthostella from the same location on 16 August 2009. A large number of individuals of each species were subsequently collected from the host Dendronephthya aff. dendritica on 20 June 2010. C. bocki can be distinguished from C. anthostella Song and Hwang, 2010 and C. komaii Utinomi, 1963 by its unique blue and orange colored stripes, and/or the branching and anastomosing milky-white stripes encircling the aboral sense organ towards the margin. The detailed morphology and molecular sequence information (nuclear 18S rDNA, internal transcribed spacer 1, and mitochondrial cox1 gene sequences) for C. bocki is provided, and C. bocki and C. anthostella are compared.

A Dual-Channel CMOS Transimpedance Amplifier Array with Automatic Gain Control for Unmanned Vehicle LADARs (무인차량 라이다용 CMOS 듀얼채널 자동 이득조절 트랜스임피던스 증폭기 어레이)

  • Hong, Chaerin;Park, Sung Min
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.5
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    • pp.831-835
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    • 2016
  • In this paper, a dual-channel feed-forward transimpedance(TIA) array is realized in a standard $0.18-{\mu}m$ CMOS technology which exploits automatic gain control function to provide 40-dB input dynamic range for either detecting targets nearby or sensing imminent danger situations. Compared to the previously reported conventional feed-forward TIA, the proposed automatic-gain-control feed-forward TIA(AFF-TIA) extends the input dynamic range 25 dB wider by employing a 4-level automatic gain control circuit. Measured results demonstrate the linearly varying transimpedance gain of 47 to $72dB{\Omega}$, input dynamic range of 1:100, the bandwidth of $${\geq_-}670MHz$$, the equivalent input referred noise current spectral density of 6.9 pA/${\surd}$HZ, the maximum sensitivity of -26.8 dBm for $10^{-12}BER$, and the power consumption of 27.6 mW from a single 1.8-V supply. The dual-channel chip occupies the area of $1.0{\times}0.73mm^2$ including I/O pads.

A Study of Applicable Strategies on the Open Source Tool in Digital Forensics (디지털 포렌식 관점에서의 오픈소스 도구 적용 방안 연구)

  • Yoon, Su-jin;Kim, Jong-bae;Shin, Yong-tae
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2014.05a
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    • pp.271-272
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    • 2014
  • As E-discovery in criminal investigation is increasing, the importance of Forensic Tools which can legally extract data with high effectiveness is getting higher. Digital products are growing fast. Therefore, Forensic Tools should be implemented readily to suit users and events well. Although forensic industry and governments use expensive forensic tools, some have suggested limitations to its use, such as memory limitations and the limits of post-audit. We need to develop open source forensic tools that can implement a variety of forensic tool fast. This research studies digital forensics technical skills which are commercialized currently and suggests applicable strategies of the open digital forensics to help overcome these limitations.

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Description of eight new mitochondrial genomes for the genus Neoarius and phylogenetic considerations for the family Ariidae (Siluriformes)

  • Luiz Guilherme Pereira Pimentel;Iuri Batista da Silva;Igor Henrique Rodrigues-Oliveira;Rubens Pasa;Fabiano Bezerra Menegidio;Karine Frehner Kavalco
    • Genomics & Informatics
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    • v.21 no.4
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    • pp.51.1-51.5
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    • 2023
  • The genus Neoarius, known as marine catfish, is a group of the family Ariidae, composed of 10 species found in Oceania. None of the species in this genus have their mitochondrial genome described, which is highly valuable in phylogenetic and molecular evolution studies. For the present work, eight species from the Neoarius genus were selected: Neoarius utarus, Neoarius midgleyi, Neoarius graeffei, Neoarius leptaspis, Neoarius berenyi, Neoarius paucus, Neoarius pectoralis, and Neoarius aff. graeffei. DNA sequences of the eight species were obtained through the NCBI Sequence Read Archive (SRA) database, and the mitochondrial genomes were assembled using the NOVOplasty tool on the Galaxy platform, subsequently annotated with the MitoAnnotator tool. We then utilized the protein-coding genes from the mitogenomes to estimate the phylogenetic relationships within the group, including seven additional mitogenomes available in the NCBI. In all species, the mitochondrial genomes presented 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 D-loop.

High-level Production of Recombinant Human IFN-$\alpha2a$ with Co-expression of $tRNA^{Arg(AFF/AGA)}$ in High-cell-density Cultures of Escherichia coli

  • Shin, Chul-Soo;Hong, Min-Seon;Shin, Hang-Chel;Lee, Jeewon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.4
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    • pp.301-305
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    • 2001
  • The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

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Effects of Perilla and Corn Oil Diets on the Hepatic Microsomal Fatty Acid Composition, Cytochrome P-450 Contents, and Glutathione S-transferase Activities in 2-Acctylaminofluorcne Treated Rats (들깨유 옥수수유의 섭취가 2-Acetylaminofluorene을 투여한 쥐 간에서 소포체막의 지방산 조성과 Cytochrome P-450 함량, Glutathione S-transferase 활성도에 미치는 영향)

  • 김경민
    • Journal of Nutrition and Health
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    • v.25 no.1
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    • pp.3-11
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    • 1992
  • This paper examines the effects of dietary fats on the fatty acid composition and market enzyme activites during liver damage in 2-acetylaminofluorene treated rats. Weaning Sprague-Dawley male rats were fed the diet of beef tallow(BT source of sturated fatty acid) corn oil(CO source of n-6 fatty acid) and perilla oil(PO source of n-3 fatty acid) at the level of 15% fat. Ten days after feeding 2-acetylaminofluorene(2-AAF) was injected intraperitoneally twice every week at the level of 50mg/kg body weight for 7 weeks. Liver microsomal and cytosolic fractions were collected to determine the microsomal fatty acid composition lipid peroxide(malondialdehyde MDA) contents glucose 6-phosphatase(G6 Pase) activity cytochrome(Cyt) P-450 contents and cytosolic glutathione S-transferase(G6 Pase) activity cytochrome(Cyt) P-450 contents and cytosolic glutathione S-transferase(GST) activity. The fatty acid composition in microsomal fraction was reflected by different dietary fats. By 2-AAF treatment linoleic acids were increased regardless of the diet MDA contents were higher in CO group than it was in BT group. However 2-AAF treatment decreased MDA contents in all dietary groups. G6Pase activity of BT group was higher than those of the other gropus. CO group had the highest Cyt P-450 contents and 2-AAF treatment lowered Cyt P-450 contents only in CO gropu GST activites were higher in CO than in BT group whereas the enzyme activites were increased by 20AAF treatment in all dietary groups. These results suggest that dietary fats and 2-AAF treatment in all dietary groups,. These results suggest that dietary fats and 2-AAF treatment affect microsomal fatty acid composition The enzyme activities concerned with liver damage were influenced differently by dietary fats and 2-AFF treatment Although PO diet contains much more polyunsaturated fatty acids than CO diet PO diet doesn't cause more oxidant stress compared with CO diet in these data.

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A Study on the Recognition & Experience of Institution Employee to the Monetary Circumstances (금융사고에 대한 금융기관 종사원의 인지 및 경험에 관한 연구)

  • Hong, Hyo-Seog;Lee, Chun-Hwa;Cho, Kook-Yeon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.2 no.2
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    • pp.23-47
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    • 2007
  • Since 1998, Korean economy has damaged financial structural coordination from business firms to financial institutions. The monetary circumstances, the faithless management in financial institution, caused an usiness shrinkage. Specially, the monetary circumstances come by every year from large to small. This dissertation examines the recognition & experience of institution employee to the monetary circumstances that underlie the announcement effects. The sample of this study consists of 201 survey which have 1st and 2nd financial institution from Gyeungnam to Seoul. To examine and identify factors that the Recognition & Experience of the Monetary Circumstances this study uses t-test, ANOVA, multiple regression. The variables SEX, AFF, FRA show expected signs and are statistically significant. The variables MAR, SCH, PER, POS, LEN and REC were turned out to be insignificant. The above results suggest that institution employee, which have male, take part lending in affairs and uneasy home status, have more recognize to the financial circumstances.

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Digestion indices of 12 species of microalgae by the oyster Crassostrea gigas larval development stages (굴, Crassostrea gigas 유생 성장단계별 미세조류 12의 소화도)

  • Hur, Young-Baek;Jeon, Chang-Young;Cho, Kee-Chae;Hur, Sung-Bum
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.359-369
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    • 2011
  • Twelve species of food microalgae were investigated to clarify the digestion index of Crassostrea gigas larvae using epifluorescence microscopy to choose an appropriate diet for artificial seed production in hatchery. An experiment was conducted using 1 (D shaped stage), 4 (Early umbo stage), 8 (umbo stage) and 12 (Full grown stage) days old larvae. larvae were stocked in 1 L flasks at 5 individuals/mL and fed $10{\times}10^4$ algal cells/mL of each species individually. Prior to larvae were fed for 3 h and then were observed under the microscope to detect ingestion; larvae were then sieved and replaced in 1 L flasks containing filtered seawater and were observed after 3, 5 and 8 h to analyse the digestion index. Values of digestion indices were specific for each alga. No evidence for the ingestion of Thalassiosira weissflogii was evident at all larval development stages tested. Digestion indices of others microalgae were 0.8-99.7% at 4 stage of larval development stages: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) and Dunaliella tertiolecta (0.0-99.6%), respectively. Therefore, it is assumed that food microalgae showing the high digestion such as I. galbana should be supplied to the early umbo stage larvae, and then after the umbo larval stage, the mixed microalgae with diatoms and light green algae should be supplied to the full grown stage larvae to increase the digestion of their larvae.

Selection of Isochrysis and Pavlova Species for Mass Culture in High Temperature Season (고온기 배양에 적합한 Isochrysis와 Pavlova 종의 선정)

  • Yang, Sung-Jin;Hur, Sung-Bum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.4
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    • pp.343-350
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    • 2012
  • Even though the microalgal species of Isochrysis and Pavlova are widely used as live food in bivalve hatcheries, they are difficult to culture in mass during the summer season. Therefore, the present study was conducted to determine the optimum species or strains of Isochrysis and Pavlova to produce good growth and high contents of fatty acids at temperatures over $30^{\circ}C$. Four species of Isochrysis (I. galbana KMMCC12, I. galbana KMMCC214, I. aff. galbana, and Isochrysis sp.) and four of Pavlova (P. lutheri, P. gyrans, P. viridis, and Pavlova sp.) were cultured at $25^{\circ}C$, $29^{\circ}C$, and $33^{\circ}C$, and then analyzed for specific growth rate and fatty acid composition. Microalgae were cultured in f/2 medium at 23 psu and continuous light of $80{\mu}mol$ photons $m^{-2}s^{-1}$. For the I. galbana, growth rates were highest at $29^{\circ}C$ and decreased at $33^{\circ}C$ to the level observed at $25^{\circ}C$. I. galbana (KMMCC12) and Isochrysis sp. cultured at $29^{\circ}C$ and $33^{\circ}C$, respectively, exhibited the highest growth rates of all Isochrysis species. In terms of fatty acids, I. galbana (KMMCC12) contained higher contents of PUFA and n-3 HUFA at $33^{\circ}C$ than did Isochrysis sp. For species of Pavlova, growth rates of P. gyrans and P. viridis at $29^{\circ}C$ and $33^{\circ}C$, respectively, were higher than those of the other Pavlova species. In particular, P. viridis grew as well at $33^{\circ}C$ as it did at $29^{\circ}C$. However, P. lutheri and Pavlova sp. did not grow at $33^{\circ}C$. In terms of fatty acids, P. viridis cultured at $33^{\circ}C$ also exhibited higher contents of PUFA and n-3 HUFA, as compared to P. gyrans. Based on these results, we suggest that I. galbana (KMMCC12) and P. viridis are suitable species for mass culture during the high temperature season.