• The Korean Journal of Pharmacology
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• v.19 no.2
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• pp.25-34
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• 1983

In this isolated study, it was aimed to elucidate the pharmacological properties of non-cholinergic, non-adrenergic inhibitory responses in the longitudinal strips of rabbit portal vein. 1) The portal vein responded inhibitory to electrical field stimulation in a frequency - and calcium-dependent manner after pretreatment with atropine, guanethidine and ergotamine, simultaneously. 2) When exogenous ATP, ADP, adenosine and cyclic AMP were added, respectively, they only showed the relaxations in the higher concentration without mimicing or affecting the inhibitory response induced by the electrical stimulation. The antagonist of purine substances, neither quinine nor isobutyl-methyl xanthine did influence on the relaxation. 3) The inhibitory response was significantly increased in the presence of $1{\mu}g/ml 4-amino-pyrineine (4-AP) which is $K^+-conduction$ blockade, but higher concentration of 4-AP directly decreased the vascular tone. 4) Though repeated application of ATP revealed the inhibitory effect on the relaxation, however, that of adenosine resulted rather increase of the amplitude. 5) After pretreatment with $^3H-adenosine$, $^3H-efflux$ induced by ATP or adenosine was markedly enhanced, but the electrical stimulation caused less $^3H-efflux$. 6) ^3H-efflux by electrical stimulation was not affected by the administration 4-AP, tetrodotoxin and adenosine.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.305-309
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• 2016

Recently, there are many efforts focused on development of more economical non-fluorinated membranes for PEMFCs (Proton Exchange Membrane Fuel Cells). In this study, to test the durability of sPEEK MEA (Membrane and Electrode Assembly), ADT (Accelerated Degradation Test) of MEA degradation was done at the condition that membrane and electrode were degraded simultaneously. Before and after degradation, I-V polarization curve, hydrogen crossover, electrochemical surface area, membrane resistance and charge transfer resistance were measured. Although the permeability of hydrogen through sPEEK membrane was low, sPEEK membrane was weaker to radical evolved at low humidity and OCV condition than fluorinated membrane such as Nafion. Performance after MEA degradation for 144 hours and 271 hours were reduced by 15% and 65%, respectively. It was showed that the main cause of rapid decrease of performance after 144 hours was shorting due to Pt/C particles in the pinholes.

• Journal of Pharmacopuncture
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• v.18 no.2
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• pp.7-18
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• 2015

Objectives: Lawsone (1,4 naphthoquinone) is a non redox cycling compound that can be catalyzed by DT diaphorase (DTD) into 1,2,4-trihydroxynaphthalene (THN), which can generate reactive oxygen species by auto oxidation. The purpose of this study was to evaluate the toxicity of the phytomarker 1,4 naphthoquinone and its metabolite THN by using the molecular docking program AutoDock 4. Methods: The 3D structure of ligands such as hydrogen peroxide ($H_2O_2$), nitric oxide synthase (NOS), catalase (CAT), glutathione (GSH), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) were drawn using hyperchem drawing tools and minimizing the energy of all pdb files with the help of hyperchem by $MM^+$ followed by a semi-empirical (PM3) method. The docking process was studied with ligand molecules to identify suitable dockings at protein binding sites through annealing and genetic simulation algorithms. The program auto dock tools (ADT) was released as an extension suite to the python molecular viewer used to prepare proteins and ligands. Grids centered on active sites were obtained with spacings of $54{\times}55{\times}56$, and a grid spacing of 0.503 was calculated. Comparisons of Global and Local Search Methods in Drug Docking were adopted to determine parameters; a maximum number of 250,000 energy evaluations, a maximum number of generations of 27,000, and mutation and crossover rates of 0.02 and 0.8 were used. The number of docking runs was set to 10. Results: Lawsone and THN can be considered to efficiently bind with NOS, CAT, GSH, GR, G6PDH and NADPH, which has been confirmed through hydrogen bond affinity with the respective amino acids. Conclusion: Naphthoquinone derivatives of lawsone, which can be metabolized into THN by a catalyst DTD, were examined. Lawsone and THN were found to be identically potent molecules for their affinities for selected proteins.

• Journal of Korean Society of Transportation
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• v.19 no.6
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• pp.207-218
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• 2001

As a city and industries are developed rapidly, a traffic accident and congestion take places on the road link become serious and it can be a large problem of the society in the future. Especially, most of the traffic accidents on the signalized intersection are caused by the human factor, vehicle and environmental factor mutually. The relation of the traffic accident and volume is acting on the outbreak of the traffic accident and the mistake of driver altogether as a major cause. The purpose of this paper is to develop a model for the forecasting of the traffic accident and to use research data gained to predict many traffic accidents. The data of this study were used with real one of the 73 areas of the four-legged signalized intersection in Kwang-ju city from 1996 to 1998 for three years to develop a model for the forecasting of the traffic accident. The statistical methods used in this paper are the principal component, regression and correlation analysis. We studied accident models to find out useful data from the statistics method and applied the data to the different area of the Choun-La province for the verification of the model. So, the result of this paper showed a reasonable model for the forecasting or the traffic accident and possibility of the model for simulating on real case. Finally, This study would be made of a study continually for the safe design and plan for the four-legged signalized intersection.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.20 no.6
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• pp.203-213
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• 2021

A vehicle crash occurs due to various factors such as the geometry of the road section, traffic, and driver characteristics. A safety performance function has been used in many studies to estimate the relationship between vehicle crash and road factors statistically. And depends on the purpose of the analysis, various characteristic variables have been used. And various characteristic variables have been used in the studies depending on the purpose of analysis. The existing domestic studies generally reflect the average characteristics of the sections by quantifying the traffic volume in macro aggregate units such as the ADT, but this has a limitation that it cannot reflect the real-time changing traffic characteristics. Therefore, the need for research on effective aggregation units that can flexibly reflect the characteristics of the traffic environment arises. In this paper, we develop a safety performance function that can reflect the traffic characteristics in detail with an aggregate unit for one hour in addition to the daily model used in the previous studies. As part of the present study, we also perform a comparison and evaluation between models. The safety performance function for daily and hourly units is developed using a negative binomial regression model with the number of accidents as a dependent variable. In addition, the optimal negative binomial regression model for each of the hourly and daily models was selected, and their prediction performances were compared. The model and evaluation results presented in this paper can be used to determine the risk factors for accidents in the highway section considering the dynamic characteristics. In addition, the model and evaluation results can also be used as the basis for evaluating the availability and transferability of the hourly model.